• Title/Summary/Keyword: Hybrid breeding

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Genome-wide analysis of heterosis-related genes in non-heading Chinese cabbage

  • Yi, Hankuil;Lee, Jeongyeo;Song, Hayong;Dong, Xiangshu;Hur, Yoonkang
    • Journal of Plant Biotechnology
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    • v.44 no.3
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    • pp.208-219
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    • 2017
  • Heterosis or hybrid vigor describes a phenomenon that superior phenotypes compared to the two parents are observed in the heterozygous $F_1$-hybrid plants. Identification and characterization of heterosis-related genes (HRGs) will facilitate hybrid breeding in crops. To identify HRGs in Brassica rapa, we analyzed transcriptome profiling using a Br300K microarray in non-heading Chinese cabbage at three developmental stages. A large number of genes were differentially expressed in $F_1$ hybrids and non-additive expression was prominent. Genes that are expressed specifically for $F_1$ hybrid at all three stages were Brassica-specific uncharacterized genes and several defense-related genes. Expression of several photosynthesis- and stress-related genes were also $F_1$ hybrid-specific. Thirteen NBS-LRR class genes showed high and specific expression in $F_1$ hybrid Shulu: some of them were characterized as defense genes in Arabidopsis, but most have not been. Further characterization of these defense-related genes in Brassica species and its application will be helpful for understanding the role of defense responses in heterosis. In addition, results obtained in this study will be valuable to develop molecular markers for heterosis and disease resistance in B. rapa.

Breeding of Purple Flower-colored Dwarf 'Jiknyeo' from Hybridization of Campanula punctata Lam. × Campanula punctata Lam. var. rubriflora Mak. (애기초롱꽃(Campanula punctata Lam.)과 자주초롱꽃(C. punctata Lam. var. rubriflora Mak.)의 교잡을 통한 자주색 꽃을 가진 왜성 초롱꽃 '직녀' 육성)

  • Choi, Nam-Hee;Kim, Gyeong-Hee;Lim, Mi-Young;Kim, Zhoo-Hyeon;Jeong, Byoung-Ryong
    • Horticultural Science & Technology
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    • v.30 no.3
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    • pp.338-341
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    • 2012
  • Campanula, native to Korea, is potentially an attractive potted or bedding plant. Experiments were conducted to produce a $F_1$ hybrid plant for a reduced plant height, 'Jiknyeo', by a conventional cross breeding between C. punctata Lam. and C. punctata Lam. var. rubriflora Mak. The crossing was carried out in July 2001 and the seeds were harvested in October 2001. Seeds were stored for two months at $5^{\circ}C$ for vernalization, and then were sown in March 2002. The $F_1$ hybrid have a purple flower color, and decreased plant height, leaf length, leaf width, and peduncle length. Each flower maintained freshness for a period of 20 days on the average. The whole flowering period of a plant was four months. The plant has flowering characteristics of an indeterminate inflorescence, prefers low humidity during the whole cultivation period, and requires low temperature in winter for flower bud differentiation.

Single Cross Maize Hybrid 'Hwangdaok' for High Grain Yield (다수성 종실용 옥수수 단교잡 신품종 '황다옥')

  • Son, Beom-Young;Baek, Seong-Bum;Kim, Jung-Tae;Lee, Jin-Seok;Bae, Hwan-Hee;Go, Young-Sam;Kim, Sun-Lim
    • Korean Journal of Breeding Science
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    • v.51 no.2
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    • pp.105-109
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    • 2019
  • 'Hwangdaok', a new maize F1 hybrid (Zea mays L.), was developed by the maize breeding team at the National Institute of Crop Science (NICS), RDA in 2016. The high-yielding yellow dent hybrid named 'Hwangdaok' was obtained by crossing between two inbred lines, 'KS203' and 'KS190'. After advanced yield trial in Suwon in 2012, regional yield trial was subsequently carried out to evaluate the growth and yield of 'Hwangdaok' at three different locations from 2014 to 2016. The number of days to silking of 'Hwangdaok' is 76. The plant height is 262 cm and ear height ratio is 51%, which are similar to those of 'Jangdaok'. It has resistance to lodging. The number of ears per 100 plants is 95. The ear length is 21.3 cm and weight of 100 seeds is 34.7 g, similar to those of 'Jangdaok'. It has moderate resistance to southern leaf blight (Bipolaris maydis) and European corn borer (Ostrinia nubilalis). The grain yield (10 ton/ha) of 'Hwangdaok' is 6% higher than that of 'Jangdaok'. The seed production rate of 'Hwangdaok' is acceptable due to good synchronization of flowering period during crossing between the seed parent, KS203, and the pollen parent, KS190, in Yeongwol. F1 seed yield was 1.68 ton/ha. 'Hwangdaok' can be a suitable cultivar to plain areas in Korea. (Variety registration No. 7502).

Variation of Nuclear DNA Content in Interspecific Allium cepa L.×A. fistulosum L. hybrids and Their Successive Backcross Lines (양파와 파간의 종간잡종 F1과 여교잡계통의 핵 DNA 함량)

  • Kim, Cheol-Woo;Kim, Hwa-Young;Lee, Eul-Tai;Choi, In-Hu;Bang, Jin-Ki
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.463-467
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    • 2009
  • Interspecific hybrid plants between Allium cepa L. (2n=2X=16) and A. fistulosum L. (2n=2X=16)and their backcross lines were developed by artificial pollination in order to introduce new desirable characters of A, cepa to A. fistulosum. The 2C nuclear DNA content has been estimated by flow cytometry in 5 Allium fistulosum inbreed lines, 2 interspecific hybrid lines of A. cepa${\times}$A. fistulosum and 34 their backcross lines $BC_1F_1$ to $BC_2F_2$, using propidium iodide (PI) as a fluorescence dye. Estimated 2C DNA values ranged from 22.2 pg to 23.7 pg in 5 A. fistulosum inbreed lines, 37.9 pg in F1 hybrid between A. cepa and A. fistulosum, 24.3 pg to 27.3 pg in 7 backcross lines in $BC_1F_1$, 21.9 pg to 24.4 pg in 9 $BC_1F_2$, 22.9 pg to 25.1 pg in 14 $BC_2F_1$, 22.6 pg to 23.4 pg in 4 $BC_2F_2$. This study showed mean 2C nuclear DNA content of $F_1$ hybrid was higher than their backcross progeny lines, while it was lower than female parental line, A. cepa (2C DNA=33.2 pg). Mean 2C DNA content of backcross lines, $BC_1F_1$ to $BC_2F_2$ was not significantly different but their 2C DNA contents in the more progress generation from $BC_1F_1$ to $BC_2F_2$ were reduced.

Breeding of Small Type and Yellow Colored Phalaenopsis "Yellow Dream" (황색계 소형 팔레놉시스 "Yellow Dream" 육성)

  • Kim, Mi-Seon;Lee, Young-Ran;Cho, Hae-Ryong;Rhee, Hae-Kyung;Lim, Jin-Hee;Park, Sang-Kun;Shin, Hak-Ki;Choi, I-Jin
    • Korean Journal of Breeding Science
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    • v.41 no.2
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    • pp.145-148
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    • 2009
  • Phalaenopsis (Phal.) "Yellow Dream" was developed by the National Institute of Horticultural & Herbal Science (NIHHS), Rural Development Administration (RDA) in 2005. This hybrid came from the progenies of a cross between Phal. "3031" which has yellow colored petals and small plant size, and Phal. "Golden Emperor" which has yellow colored petals with red spots and small plant size in 1995. "Yellow Dream" was finally selected in 2005 after the investigation of the growth and flower characteristics from 2000 to 2004. "Yellow Dream" had a yellow (Y4D) base color and light orange stripe petals (ORN34A) with a red lip (R42D). The average leaf length and flower width of "Yellow Dream" were 21cm and 6.8cm, respectively. It has a half erect leaf form and is a fast growing cultivar. This hybrid is relatively easy to make clones.

Nutrition, Utilization and Productivity of Pearl Millet Hybrids Developed in Korea (진주조의 영양과 이용 및 생산성)

  • Keun-Yong Park;Rae-Kyung Park;Byeong-Han Choi
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.1
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    • pp.103-114
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    • 1994
  • Pearl millet is a $C_4$ plant and summer crop originated from west Africa, and the sixth most important cereal in the world and the most widely cultivated millet in the semi-arid tropics as a major staple food crop. Its grain of higher quality protein is used to make unleavened bread chapatis and prepared as gruel, dumplings, couscous and beer. It is also used as animal feed and forage in both temperate and tropical regions because it has a capability to grow well not only in the fertile soil, but also in the poor and dry soil. Most of the current breeding procedures used in pearl millet are aimed at maximum exploitation of hybrid vigor for both grain and forage yields in Korea. Pearl millet is ideally suited for exploitation of heterosis using cytoplasmic male sterile lines as seed parent, and fertile inbred lines and open-pollinated cultivars as pollen parent. Pearl millet hybrids developed in Korea produced 3 to 7 tons of grain and 100 to 150 tons of green fodder per hectare.

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Estimation of Hybrid Vigor of Some Egyptian Single Local Hybrids of Mulberry Silkworm, Bombyx mori L.

  • Ghazy, Usama Mohamed Mohamed
    • International Journal of Industrial Entomology and Biomaterials
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    • v.25 no.2
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    • pp.147-151
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    • 2012
  • Fifteen races resulted from silkworm breeding program at Sericulture Research Department (SRD) were used for hybridization. Fourteen hybrids were obtained and coded as; Giza C, D, R, S, T, U, A, V, W, P, H, L and Qanater 1, 2. The traits of cocoon weight, cocoon shell weight, pupal weight, cocoon shell ratio, silk productivity, fifth instar duration, total larval duration, number of cocoon per liter and pupation ratio were evaluated. Data were analyzed by using three formulae of heterosis over better, mid and check parent values. Hybrids of Giza V, C, N, Qanater 1 and 2 are promising and could be used in commercial cocoon production. Generally, there are some new hybrids can be exploited in commercial scale. Also, the local races can be evolved using the hybridization, inbreeding and selection program.

Characteristics and Yield of Recommended Cultivars by Imported Forage Crop Regional Yield Trials in 2002 I. Early Maturing, Good Qualify, and High Yield of Forage Corn Hybrid, “DK 537 (2002년 사료작물 수입적응성 인증품종의 생육기성 및 수동성 1. 조숙 양질 다수성 사료작물 옥수수 교잡종 “DK 537”)

  • Sung, B.R.;Choi, G.J.;Lim, Y.W.;Lim, Y.C.;Park, K.J.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.22 no.4
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    • pp.247-252
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    • 2002
  • “DK 537”has been selected by forage crop breeding team at the National Livestock Research Institute(NLRI), RDA and has been determined by the Deliberative Council of the National Agricultural Cooperative Federation(NACF) in 2002, as a new recommended hybrid which is early maturing, good quality and hi호 yield of corn for silage. The characteristics of this hybrid are as follows ; 1. The seed coat is yellow and the mean of tasseling date is 5th of July, included in early maturing hybrid which is one day delayed than check cultivar, DK 501. The culm length is 246cm. It is resistant to lodging because of its low height from surface to ear. 2. DK 537 shows resistance to H. maydis and Maize Black-Streaked Dwarf Virus(MBSDV). It also shows strength to corn borer as much as DK 501 does. 3. Fresh yield. dry matter yield. and TDN per a ha are 50 tons, 16.9 tons, and 11.4 tons respectively. which are almost same yielding level of DK 501. Its percent ear to total dry matter is 50.8% at the same time. Through all these tests, we could make sure at DK 537 hybrid will be recommended as good forage crop.

Changes of Morphological and Growth Characteristics Collected Miscanthus Germplasm in Korea (국내 억새 유전자원 수집 후 형태 및 생육 특성 변화)

  • Song, Yeon-Sang;Lee, Ji-Eun;Moon, Youn-Ho;Yu, Gyeong-Dan;Choi, In-Seong;Cha, Young-Lok;Kim, Kwang-Soo
    • Weed & Turfgrass Science
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    • v.7 no.1
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    • pp.22-34
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    • 2018
  • Miscanthus has been considered as the most promising bioenergy crop for lignocellulosic biomass production. In Korea, M. sacchariflorus and M. sinensis can be found easily in all regions. It is a great advantage to utilize as important species with respect to genetic and cross-breeding programs materials for creation of novel hybrids. For successful breeding programs, it is important to precisely understand the variability of morphological and growth characteristics among Miscanthus species as breeding parent materials. In this study, morphological and growth characteristics were observed in 960 germplasms of two Miscanthus species (M. sacchariflorus and M. sinensis) for growing seasons over three years. Due to the inherent characteristics of these species, the germplasm of M. sacchariflorus among the collected germplasm were reduced in plant height than in the collection area. In M. sinensis, the plant height of germplasm collected mainly from Jeju-do increased more than those collected from collection area. Sixty-one of the collected 960 germplasms were selected and investigated to the morphological characteristics. Based on the investigated morphological data, the phylogenic tree was developed. As the results, it was confirmed that there exist germplasm in which the characteristics of M. sacchariflorus and M. sinensis are mixed. This study of Miscanthus may provide an important information in order to expedite the introduction as breeding materials for creation of new hybrid.