• Journal of Microbiology and Biotechnology
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• 제18권10호
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• pp.1695-1700
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• 2008

An immunosensor based on surface plasmon resonance (SPR) with enhanced performance was developed through a mixed self-assembled monolayer. A mixture of 16-mercaptohexadecanic acid (16-MHA) and 1-undecanethiol with various molar ratios was self-assembled on gold (Au) surface and the carboxylic acid groups of 16-MHA were then coordinated to Zn ions by exposing the substrate to an ethanolic solution of $Zn(NO_3)_2\cdot 6H_2O$. The antibody was immobilized on the SPR surface by exposing the functionalized substrate to the desired solution of antibody in phosphate-buffered saline (PBS) molecules. The film formation in series was confirmed by SPR and atomic force microscopy (AFM). The functionalized surface was applied to develop an SPR immunosensor for detecting human serum albumin (HSA) and the estimated detection limit (DL) was 4.27 nM. The limit value concentration can be well measured between ill and healthy conditions.

• Journal of Microbiology and Biotechnology
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• 제27권12호
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• pp.2228-2236
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• 2017

During menopausal transition, the imbalance of estrogen causes body weight gain. Although gut microbiome dysbiosis has been reported in postmenopausal obesity, it is not clear whether there is any difference in the microbiome profile between dietary-induced obesity and postmenopausal obesity. Therefore, in this study, we analyzed intestinal samples from ovariectomized mice and compared them with those of mice with high-fat diet-induced obesity. To further evaluate the presence of menopause-specific bacteria-gene interactions, we also analyzed the liver transcriptome. Investigation of the 16S rRNA V3-V4 region amplicon sequence profile revealed that menopausal obesity and dietary obesity resulted in similar gut microbiome structures. However, Bifidobacterium animalis was exclusively observed in the ovariectomized mice, which indicated that menopausal obesity resulted in a different intestinal microbiome than dietary obesity. Additionally, several bacterial taxa (Dorea species, Akkermansia muciniphila, and Desulfovibrio species) were found when the ovariectomized mice were treated with a high-fat diet. A significant correlation between the above-mentioned menopause-specific bacteria and the genes for female hormone metabolism was also observed, suggesting the possibility of bacteria-gene interactions in menopausal obesity. Our findings revealed the characteristics of the intestinal microbiome in menopausal obesity in the mouse model, which is very similar to the dietary obesity microbiome but having its own diagnostic bacteria.

• 한국정밀공학회지
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• 제24권1호
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• pp.118-125
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• 2007

In this study, we analyzed the plantar shear stress and normal pressure in lateral heel diabetic foot patients during walking by using in-shoe local shear stress and plantar pressure measurement systems. The shear force transducer based on the magnetic-resistive principle, was a rigid 3-layer circular disc. Shear transducers were mounted on the locations of four metatarsal heads and heel in the insole. Twelve normal subjects and three diabetic foot patients with diabetic neuropathy in the lateral heel participated in this study. The center of pressure in lateral heel diabetic foot patients moved more medially and directed toward the first, medial to the second metatarsal heads, and the hallux during late stance, making pressure at the medial heel and the second metatarsal head significantly larger than in the normal. Shear stress at the heel changed significantly in early stance and the magnitude of shear stresses in each metatarsal head also changed. Further studies would be very helpful to design foot orthoses in patients with diabetic neuropathy or other diseases.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2011년도 제42회 하계학술대회
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• pp.143-144
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• 2011

Due to enhanced demands on quality, security and reliability of the electric power energy system, a microgrid has become a subject of special interest. In this paper, output characteristics of energy storage system (ESS) with an electric double layer capacitor (EDLC) and battery energy storage system (BESS) of a renewable energy based microgrid were analyzed under grid-connected and islanded operation modes. The microgrid which consists of photovoltaic and wind power turbine generators, diesel generator, ESS with an EDLC, BESS and loads was modeled using real time digital simulator. The results present the effective control patterns of the microgrid system.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2008년도 춘계 학술발표회 초청강연 및 논문집
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• pp.734-741
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• 2008

At-rest and active earth pressure in plane strain condition have been applied to the design of cylindrical retaining walls. But many researchers have indicated that the earth pressure on the cylindrical retaining walls would be smaller than in plane strain condition due to wall deformation and stress relief. In this paper, the distribution of earth pressure acting on diaphragm wall of a shaft in dry sand was predicted by using the convergence confinement method and model test was performed to verify the estimated values. Test results showed that the earth pressure acting on the diaphragm wall of a shaft was expected to be 1.1~1.5 times larger than active earth pressure of plane strain condition and 0.7~0.9 times less than at-rest earth pressure.

• 환경위생공학
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• 제21권2호
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• pp.69-83
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• 2006

This research aims to improve the environmental education for elementary student's education program. That let environmental education be connected to other curriculum with a compunction enhancement plan to the internal volume side of environmental education, and grope for activation of compunction environmental education with curriculum necessary paradigm conversion of education for continuance possibility in green education. In case of legal and system, improving of legal and system by establishment of the environmental education act for system repair and source of revenue preparation of environmental education is needed. Based on importance of teaching-education and evaluation, it must be developed various teaching-education ways of environmental education in case of curriculum and an improvement of teaching-education ways and evaluation must be performed. After teaching-education about environmental education has been executed, a teaching-education improvement must be performed by evaluation.

• 한국직업건강간호학회지
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• 제21권1호
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• pp.10-17
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• 2012

Purpose: The study was done to identify relations among sense of humor, self-esteem, and performance in nurses. Methods: Data were collected by questionnaires from 198 nurses in Seoul and Gyeonggi province. Measures were sense of humor, self-esteem, and performance. Data were analyzed using Pearson correlation coefficients, t-test, ANOVA, and stepwise multiple regression with SPSS/WIN 13.0 version. Results: This study shows that performance of nurses has significant difference according to age, marriage, education level, income and clinical experience. The performance of nurses has positive correlation with their sense of humor and self-esteem. The higher clinical experience, sense of humor, and self-esteem they have, the better performance they make. The factors that affect performance of nurses are clinical experience, sense of humor and self-esteem. The degree of explanation is 36.5%. Conclusion: Knowing that sense of humor and self-esteem of nurse are significant factors which can cause nurses' better performance, we suggest an education program that can help nurses to develop and manage their sense of humor.

• 한국수산과학회지
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• 제47권2호
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• pp.174-178
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• 2014

In this study, 6-10 clinically healthy farmed abalone, Haliotis discus hannai were obtained from Jeollanam-Do Wando Soan-myeon, Bogil-myeon, and Nohwa-eup monthly for 1 year 7 months and were examined histopathologically. As a result, the adductor muscle had severe cellular swelling and myonecrosis. These lesions were stained with hematoxylin and eosin and Giemsa. The lesions were not caused by infection of bacteria or parasites. We investigated the relationship between lesion frequency and water temperature and discovered that increased water temperature was associated with increasing lesion frequency. As water temperature is related to growth rate, increased growth rate was closely related to increased lesion frequency. We considered that these lesions could be a useful factor in measuring shellfish health.

• Journal of Korean Neurosurgical Society
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• 제50권5호
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• pp.403-408
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• 2011

Objective : Contrary to some clinical belief, there were quite a few studies regarding animal models of intracerebral hemorrhage (ICH) $in$ $vivo$ suggesting that prior use of statins may improve outcome after ICH. This study reports the effect of 3-hydroxy-3-methyl-glutaryl-coenzyme A (HMG CoA) reductase inhibitor, simvastatin given before experimental ICH. Methods : Fifty-one rats were subjected to collagenase-induced ICH, subdivided in 3 groups according to simvastatin treatment modality, and behavioral tests were done. Hematoma volume, brain water content and hemispheric atrophy were analyzed. Immunohistochemical staining for microglia (OX-42) and endothelial nitric oxide synthase (eNOS) was performed and caspase-3 activity was also measured. Results : Pre-simvastatin therapy decreased inflammatory reaction and perihematomal cell death, but resulted in no significant reduction of brain edema and no eNOS expression in the perihematomal region. Finally, prior use of simvastatin showed less significant improvement of neurological outcome after experimental ICH when compared to post-simvastatin therapy. Conclusion : The present study suggests that statins therapy after ICH improves neurological outcome, but prior use of statins before ICH might provide only histological improvement, providing no significant impact on neurological outcome against ICH.

• 제어로봇시스템학회논문지
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• 제12권10호
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• pp.955-961
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• 2006

The global ultrasonic system for the self-localization of a mobile robot consists of several ultrasonic transmitters fixed at some reference positions in the global coordinates of robot environment. By activating the ultrasonic transmitters, the mobile robot is able to get the distance to the ultrasonic transmitters and compute its own position in the global coordinate. Due to the limitation on the ultrasonic signal strength and beam width as well as the environmental obstacles however, the ultrasonic signals from some generator may not be transmitted to the robot. Thus, instead of activating the all ultrasonic transmitters, it is necessary to select some ultrasonic generators to activate based on the current robot position. In this paper, we propose a selective activation algorithm for self-localization with the global ultrasonic system. The selective activation algorithm gets the meaningful ultrasonic data at every sampling instants, which results in the faster and more accurate response of the self-localization than the conventional sequential activation. Through the self-localization and path following control, we verify the effectiveness of the proposed selective activation algorithm.