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Development of Surface Plasmon Resonance Immunosensor through Metal Ion Affinity and Mixed Self-Assembled Monolayer  

Lee, Si-Ra (Department of Chemical and Biomolecular Engineering, Sogang University)
Sim, Sang-Jun (Department of Chemical Engineering, Kwangwoon University)
Park, Chul-Hwan (College of Life Sciences and Biotechnology, Korea University)
Gu, Man-Bock (Department of Bio-Chemical Engineering, Dongyang University)
Hwang, Un-Yeon (School of Chemical and Biological Engineering, Seoul National University)
Yi, Jong-Heop (Interdisciplinary Program of Integrated Biotechnology, Sogang University)
Oh, Byung-Keun (Department of Chemical and Biomolecular Engineering, Sogang University)
Lee, Jin-Won (Department of Chemical and Biomolecular Engineering, Sogang University)
Publication Information
Journal of Microbiology and Biotechnology / v.18, no.10, 2008 , pp. 1695-1700 More about this Journal
Abstract
An immunosensor based on surface plasmon resonance (SPR) with enhanced performance was developed through a mixed self-assembled monolayer. A mixture of 16-mercaptohexadecanic acid (16-MHA) and 1-undecanethiol with various molar ratios was self-assembled on gold (Au) surface and the carboxylic acid groups of 16-MHA were then coordinated to Zn ions by exposing the substrate to an ethanolic solution of $Zn(NO_3)_2\cdot 6H_2O$. The antibody was immobilized on the SPR surface by exposing the functionalized substrate to the desired solution of antibody in phosphate-buffered saline (PBS) molecules. The film formation in series was confirmed by SPR and atomic force microscopy (AFM). The functionalized surface was applied to develop an SPR immunosensor for detecting human serum albumin (HSA) and the estimated detection limit (DL) was 4.27 nM. The limit value concentration can be well measured between ill and healthy conditions.
Keywords
Mixed self-assembled monolayer; surface plasmon resonance; immunosensor; metal ion; human serum albumin;
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Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 3  (Related Records In Web of Science)
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