• Archives of Plastic Surgery
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• 제35권3호
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• pp.229-234
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• 2008

Purpose: There are some reports presenting that peripheral blood contain circulating hematopoietic cells as well as, in significantly smaller quantities, mesenchymal stem cells. The purposes of this study is to isolate and characterize circulating mesenchymal progenitor cells with osteogenic potential from human peripheral blood. Methods: Human buffycoat containing mononuclear cells was harvested from peripheral blood of normal persons and isolated using a density gradient centrifugation and serially subcultured in osteogenic media for 1-4 weeks. The proliferation capability, phase-contrast microscopy, transmission electron microscopy, immunophenotype FACS analysis, Alizarin red staining and RT-PCR assays for osteogenic differentiation potential were performed. Results: The phenotype of cultured cells changed from small round or cuboidal cells at passage 1 into large spindle-shaped fibroblastic morphology cells at passage 4. Surface marker expressed CD14, but did not express CD34, CD80, CD83. Strong positive staining was observed for Alizarin reds in osteogenic medium on day 14, Using RT-PCR, the mRNA levels of bone- specific genes, such as ALP, c-bfa-1 and osteocalcin were detected. Conclusion: A new subset of peripheral blood derived progenitor cells described here has the ability to proliferate and differentiate into osteogenic cell lineages in vitro, and to be candidate for regenerative therapy.

• 약학회지
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• 제49권4호
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• pp.340-346
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• 2005

To investigate the effect of Feelch on alcohol metabolism, we measured both blood alcohol concentration in human and hepatic alcohol metabolizing enzyme activity in rats. The blood alcohol concentration in Feelch-ingested group was significantly lower than that in water-ingested group at 0, 40, and 80 minute after alcohol intake. The blood alcohol concentration between male and female taken 300ml of $21\%$ alcohol showed the significant differences; the peak value of blood alcohol concentration in male and female were $0.083\pm0.014\%\;and\;0.108\pm0.018\%$, respectively. In alcohol-fed rats, aldehyde dehydrogenase (ALDH) activity was significantly increased, whereas alcohol dehydrogenase (ADH) activity was not changed. In both Feelch-fed group and Feelch plus alcohol-fed group, ADH and ALDH activity were significantly increased as compared with each control group. Feelch decreased phospholipase $A_2$ activity and lipid peroxidation in hepatic tissue and activities of serum aminotransferases as compared with control. These results suggest that Feelch may have a hepatoprotective effects and this is likely due to lower blood alcohol concentration via the increment of hepatic ADH and ALDH activity.

• 대한수의학회지
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• 제6권1호
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• pp.57-75
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• 1966

This study was conducted to observe the condition of the ramifications of the bronchus and pulmonary blood vascular system by injecting the vinylite into the bronchial tree and pulmonary blood vessels in 100 normal adult dogs. The results obtained were summarized as follows: 1. Lungs of dog were composed of the same pulmonary territories as in lungs of human. 2. Cardiac lobe corresponding to R.medio-bassalis of human lungs was well developed and situated as a independent cardiac lobe, in ventral side of right lung. 3. Bronchial tree were in the patterns of axial divergency and blood vascular systems were (in general) branched along the bronchial tree, arteries lying near the bronchial tree but veins apart from it. 4. Among the branching patterns of bronchus pulmonary artery and pulmonary vein in each lobe, the type presented most frequently were noted, which were designated basic type by the author. 5. Pulmonary blood vessels were not always branched in accordance with bronchial tree, diverged inmore complex patterns, especially in venous vascular system. 6. Ramus anterior (lobe apicalis) was always observed in all casting specimen. 7. There was a case of peculiar variation patterns of the ramification in the bronchi directing into the left apical and cardiac lobe, arose respectivelly, at independent origin of bifurcation in the left bronchial stem, and a case of peculiar variation pattern of the artery entering left apical lobe and cardiac lobe, had a same origin of the bifurcation at rami pulmonary artery, and then divided respectivelly into the rami medi artery and rami left apical lobe artery. 8. In the classification based on the patterns of bronchial and blood vasculor divergencys, there were a lot of significant combination cases in their patterns.

• Asian Pacific Journal of Cancer Prevention
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• 제17권1호
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• pp.235-238
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• 2016

Hepatitis C is an ailment of liver caused by hepatitis C virus (HCV) infection. About 3% of the world population is infected by this virus. HCV infection is a leading reason for liver cirrhosis and therefore a major source of hepatocellular carcinoma. The study focused on the incidence of active HCV infection in blood donors of Mardan district of KPK, Pakistan. A total of 5318 blood donors were inspected for the presence of anti-HCV antibodies and HCV-RNA using ICT (immune-chromatographic test), ELISA and RT-PCR at Mardan Medical Complex (MMC), Mardan. Out of these, 157 (2.95%) were positive by ICT, 60 (1.12%) by ELISA and 56 (1.05%) for HCV-RNA. The frequency of active HCV infectivity amongst the blood donors from district Mardan, KPK Pakistan was 1.05 %. Application of strict measures during blood donor selection and use of proper screening assays such as ELISA in place of ICT devices can give a more accurate picture so that the incidence of this viral infection in HCV negative blood recipients can be reduced.

• BMB Reports
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• 제50권10호
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• pp.504-509
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• 2017

Ischemia is a serious disease, characterized by an inadequate blood supply to an organ or part of the body. In the present study, we evaluated the effects of the anti-microbial peptide SR-0379 on the stem cell-mediated therapy of ischemic diseases. The migratory and tube-forming abilities of human endothelial progenitor cells (EPCs) were enhanced by treatment with SR-0379 in vitro. Intramuscular administration of SR-0379 into a murine ischemic hindlimb significantly enhanced blood perfusion, decreased tissue necrosis, and increased the number of blood vessels in the ischemic muscle. Moreover, co-administration of SR-0379 with EPCs stimulated blood perfusion in an ischemic hindlimb more than intramuscular injection with either SR-0379 or EPCs alone. This enhanced blood perfusion was accompanied by a significant increase in the number of CD31- and ${\alpha}$-SMA-positive blood vessels in ischemic hindlimb. These results suggest that SR-0379 is a potential drug candidate for potentiating EPC-mediated therapy of ischemic diseases.

• 한국가정과학회지
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• 제7권2호
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• pp.1-11
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• 2004

The purpose of this study was designed to observe the effects of the feeding Cynanchum wilfordii extract on the improvement of the blood glucose, lipid components in the serum of dietary hyperlipidemic and streptozotocin(STZ) -induced diabetic rats(S.D. strain, ♂) fed the experimental diets for 5 weeks. Concentrations of total cholesterol, atherosclerotic index, LDL, LDL-Cholesterol, free-cholesterol. cholesteryl ester, TG, PL and blood glucose in serum were significantly higher in the cholesterol administration groups((group 2(cholesterol+water), 4(cholesterol+Cynanchum WIlfordii 3.5g% extract)) than those in the control group (group1 , basal diet+water). But the concentrations of total cholesterol. atherosclerotic index, LDL, LDL- cholesterol. free-cholesterol, cholesteryl ester, TG, PL and blood glucose in serum were remakably lower in the group 4 than those in the group 2. In the STZ(55mg/kg B.W.)-induced diabetic groups((group 3(STZ, IP.)+water), 5(STZ(IP.)+Cynanchum WIlfordii 3.5g% extract? the serum total cholesterol, atherosclerotic index, LDL, LDL-cholesterol, free-cholesterol. cholesteryl ester, TG, PL and blood glucose concentrations actions were rather lower in the group 5 than those in the group 3. In the ratio of HDL -cholesterol concentration to total cholesterol and HDL-cholesterol concentration, Cynanchum wilfordii extract administration groups were higher percentage than III the groups 2 and 3. The activities of aspartate aminotransferase(AST), alanine aminotransferase(ALT), alkaline phosphatase (ALP) and lactate dehydrogenase(LDH) in serum were rather lower in the Cynanchum wllfordii extract administration groups (group 4,5) than in the cholesterol diet group(group 2) and STZ-induced diabetic group (group 3). From the above research, the physiological activity substances in Cynanchum wllfordii were effective on the improvement of the blood glucose, lipid compositions in serum of dietary hyperlipidemic and STZ-induced diabetic rats. And particularly, physiological activity substance in Cynanchum wilfordii was more effective therapeutic regimen for the control of metabolic derangements in adult disease.

• 한국환경성돌연변이발암원학회지
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• 제25권1호
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• pp.13-18
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• 2005

In the present study, we have measured 8-hydroxy-2'-deoxyguanosine in DNA of stomach cancers, adjacent stomach cancer tissues, normal stomach tissues and peripheral blood leukocytes of the same stomach cancer patients (n = 48) to investigate their etiological association with gastric cancer and possibility whether peripheral blood leukocytes can use surrogate marker for early stomach cancer diagnosis by HPLC/ECD system. In normal stomach tissues, we found that 8-hydroxy-2'-deoxyguanosine levels in tissues infected with Helicobacter pylori were 1.4 fold higher than those in tissues without infected with Helicobacter pylori. However, in adjacent stomach cancer tissues, we found that 8-hydroxy-2'-deoxyguanosine levels in tissues infected with Helicobacter pylori were 1.5 fold lower than those in tissues without infected with Helicobacter pylori. In stomach cancer tissues, 8-hydroxy-2'-deoxyguanosine levels in tissues infected with Helicobacter pylori were not significantly different from those in tissues without infected with Helicobacter pylori. In Helicobacter pylori-negative specimens, 8­hydroxy-2'-deoxyguanosine levels of adjacent stomach cancer tissues were found to be significantly higher than those of normal stomach and cancer tissues. The 8-hydroxy-2'-deoxyguanosine levels of female were 1.7 fold higher than those of male in peripheral blood leukocytes of the same stomach cancer patients. The 8-hydroxy-2'­deoxyguanosine levels in Helicobacter pylori-negative specimens among adjacent stomach cancer tissues were found to be reversely correlated with those in peripheral blood leukocytes, suggesting that 8-hydroxy-2'-deox­yguanosine in peripheral blood leukocytes may not use as surrogate marker for the early diagnosis of human stomach cancer.

• Annals of Laboratory Medicine
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• 제38권6호
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• pp.599-603
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• 2018

Frequencies of red blood cell (RBC) blood group antigens differ by ethnicity. Since the number of immigrants is increasing in Korea, RBC antigens should be assessed in children/youths with parents of different ethnicities to ensure safe transfusions. We investigated the frequency of RBC antigens, except for ABO and RhD, in 382 children and youths with parents having Korean and non-Korean ethnicities. Subjects were divided into those with ethnically Korean parents (Korean group; N=252) and those with at least one parent of non-Korean ethnicity (non-Korean group; N=130). The 37 RBC antigens were genotyped using the ID CORE XT system (Progenika Biopharma-Grifols, Bizkaia, Spain). The frequencies of the Rh (E, C, e, $hr^S$, and $hr^B$), Duffy ($Fy^a$), MNS ($Mi^a$), and Cartwright ($Yt^b$) antigens differed significantly between the two groups. Eight and 11 subjects in the Korean and non-Korean groups, respectively, exhibited negative expression of high-frequency antigens, whereas 14 subjects in the non-Korean group showed positive expression of low-frequency antigens. The frequency of RBC antigens has altered alongside demographic changes in Korea and might lead to changes in distribution of RBC antibodies that cause acute or delayed hemolytic transfusion reaction.

• Current Optics and Photonics
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• 제3권1호
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• pp.46-53
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• 2019

Monte Carlo simulations were performed for a three-dimensional tissue model with and without an embedded large vessel, to understand how varying vessel geometry affects surface light distribution. Vessel radius was varied from 1 to 5 mm, and vessel depth from 2 to 10 mm. A larger difference in surface fluence rate was observed when the vessel's radius increased. For vessel depth, the largest difference was seen at a depth of approximately 4 mm, corresponding to human wrist region. When the vessel was placed at depths greater than 8 mm, very little difference was observed. We also tested the feasibility of using two source-detector pairs, comprising two detectors distinctly spaced from a common source, to noninvasively measure blood-scattering changes in a large vessel. High sensitivity to blood-scattering changes was achieved by placing the near detector closer to the source and moving the far detector away from the source. However, at longer distances, increasing noise levels limited the sensitivity of the two-detector approach. Our results indicate that the approach using two source-detector pairs may have potential for quantitative measurement of scattering changes in the blood while targeting large vessels near the human wrist region.

• 한국식생활문화학회지
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• 제21권2호
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• pp.216-224
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• 2006

This study was conducted to examine the correlation among meals variety, obesity index, blood pressure, and lipid profiles of Korean adults. A total of 308 adults(men 124, women 184) measured the anthropometric characteristics, blood pressure, dietary intake using 24-hour recall method, and serum lipid profile. The average ages of the study subjects were 50.9 yrs in men and 51.4 yrs in women. The average height, weight and BMI were 166.3 cm, 68.4 kg, $24.7\;kg/cm^2$ in men and 154.3 cm, 59.8 kg, $25.0\;kg/cm^2$ in women, respectively. The numbers of food items in meal of men and women were 14.1, 15.9 for breakfast, 15.7, 15.9 for lunch, 14.7, 14.1 for dinner, respectively. The numbers of dish items in meal of men and women were 4.0, 4.3 for breakfast, 4.3, 4.3 for lunch, 4.2, 3.9 for dinner, respectively. The average blood pressure, total cholesterol, triglyceride, HDL-cholesterol, LDL-cholesterol and atherogenic index were 126.6/76.0 mmHg, 181.0 mg/dL, 157.7 mg/dL, 40.9 mg/dL, 108.6 mg/dL, 3.5 in men and 123.2/73.6 mmHg, 185.0 mg/dL, 137.3 mg/dL, 44.8 mg/dL, 112.7 mg/dL, 3.2 in women, respectively. The total cholesterol was negatively correlated to the number of food item for lunch(p<0.05). Therefore, more systematic studies to investigate the meal variety roles of blood lipids in meals of adults were required.