• Korean Journal of Fisheries and Aquatic Sciences
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• v.44 no.5
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• pp.456-463
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• 2011

We attempted to isolate a collagenase-1 inhibitory peptide from skate Dipturus chilensis skin protein. The protein from skate skin was digested by various enzymes (alcalase, ${\alpha}$-chymotrypsin, neutrase, papain, pepsin, and trypsin) to produce a collagenase-1 inhibitory peptide. The collagenase-1 inhibitory activity of the peptides obtained was measured by gelatin digestion assay. Among the six hydrolysates, pepsin hydrolysate exhibited the highest collagenase-1 inhibitory activity. The peptide showing strong collagenase-1 inhibitory activity was purified by Sephadex G-25 gel chromatography and HPLC using an octadecylsilyls (ODS) column. The amino acid sequence of purified collagenase-1 inhibitory peptide was identified to be Asn-Leu-Asp-Val -Leu-Glu-Val-Phe (961 Da) by quadrupole time of flight (Q-TOF) and electrospray ionization mass spectrometry (ESI-MS) mass spectroscopy. The $IC_{50}$ value of purified peptide was 87.0 ${\mu}M$. Moreover, the peptide did not exhibit cytotoxic effects on human dermal fibroblast cell lines.

• International Journal of Oral Biology
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• v.41 no.1
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• pp.33-38
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• 2016

One of functions of Galla Rhois (GR) is reportedly an anti-inflammatory effect on the several inflammatory diseases. However, an effect of GR related to periodontitis has not been investigated. In the present study, we examined the effect of the hexane extract of Galla Rhois (GR-H) on periodontitis. Cytotoxicity was assessed by MTS analysis using human gingival fibroblast (hGF) cells. Experimental periodontitis was induced by injecting E.coli LPS into the palatal gingiva maxillary molar thrice weekly for 3 weeks (LPS group). GR-H diluted in 1xPBS was orally administrated using a syringe at 30 mg/kg body weight and 100 mg/kg body weight once a day (GR-H group). GR-H effect on the alveolar bone loss (ABL) was digitized with a micro-CT. GR-H treatment at concentrations exceeding 0.5 mg/ml showed cytotoxic effect in hGF cells. The micro-CT among groups were presented for the different distances from cemento-enamel junction (CEJ) to alveolar bone crest (ABC). The results indicated an inhibitory effect on alveolar bone loss for orally administered GR-H in a model of LPS-induced periodontitis.

• Korean Journal of Medicinal Crop Science
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• v.21 no.1
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• pp.54-60
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• 2013

According to previous reports, antioxidant activities of Codonopsis lanceolata could be increased by a steaming process. This study was performed to improve its antioxidant activity and skin whitening activities of C. lanceolata by high pressure and stepwise steaming complex process. The complex processed C. lanceolata showed highest free radical scavenging acitivity as 45.21%, and for phenol and flavonoid contents, complex processed C. lanceolata contained higher than those from conventional extraction process or steaming process alone. The Cytotoxicity of all C. lanceolata extracts also showed low cytotoxicity against human fibroblast cell (CCD-986sk) as 4.49 ~ 10.40%. In whitening activity, high inhibition of tyrosinase activity was estimated as 25.08% by adding the extracts from complex process. We found that whitening and antioxidant activity of complex processed C. lancolata extract was higher than those obtained from conventional extraction and a steaming process because various kinds of antioxidant compounds could be easily released by combined process, compared to one of each process.

• Restorative Dentistry and Endodontics
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• v.14 no.1
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• pp.25-40
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• 1989

This study was conducted to evaluate the influence of 5 microfilled composite resin to fibroblast cultivated from human pulp (age 13). Each composite resin was manually mixed and filled in cylinder. Resin filled cylinders were placed in dishes (35mm in diameter) containing 3 ml of ${\alpha}$-MEM. Filters (pore size 0.22 ${\mu}m$) to simulate dentin were also placed between the bottom of cylinder and the dish floor. Then stored in 5% $CO_2$ containing incubator for 1 and 2 weeks at the temperature 36.6 C. The results analysed after 1 and 2 weeks were as follows: 1. Experimental group except group 2, 2 weeks incubation cases showed the cytotoxicity compared to the control group in cell count. 2. After 2 week-incubation of group 1 and group 4, cell count was more decreased than 1 week cases and cytotoxicity seemed to be constantly influenced to the cell multiplication. 3. The cell growth rate of 1 week incubation in group 3 and group 5 was similar to the control group and recognized the cytotoxicities of these groups were mild. 4. The cell multiplication rate of 2 week incubation cases in group 2 was greater than control group.

• Proceedings of the KOSOMBE Conference
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• v.1989 no.05
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• pp.7-8
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• 1989

The graft copolymer of chitosan with amino acid, L-lysine was synthesized by heterogeneous copolymerization and was evaluated as an artificial skin. The mechanical properties under dry and wet state, water content, water vapor transmittance rate and biodegradability were measured. The tensile strength and elongation under wet state ranged $0.3-0.5\;kg/mm^2$, 10-13%, respectively. Water vapor transmittance rate ranged $450-500\;g/m^2{\cdot}day$ like that of the normal skin. The weight loss of prepared membrane by protease IV was measured for the degree of biodegradation. The degree of biodegradation was around 15% and after 4 days it was slow. Biocompatibility was evaluated by studying the attachment of human fibroblast on the prepared membrane surface.

• Toxicological Research
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• v.17 no.4
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• pp.297-301
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• 2001

The acute toxicity of CJ-50005, an inactivated whole virus vaccine derived from hepatitis A virus (HM175) grown in human MRC-5 diploid fibroblast culture, was tested in Sprague Dawley (SD) rats and beagle dogs. CJ-50005 was orally and intramuscularly administered up to the maximum dose of 81$\mu\textrm{g}$/kg. as much as 3,000 times of the expected clinical dose, in SD rats and was intramuscularly administered up to 27 $\mu\textrm{g}$/kg, as much as 1,000 times of the expected clinical dose, in beagle dogs. In these experiments, there were no death and clinical changes which were related to CJ-50005 administration. In addition, there were no significant changes between control and treated groups in body weights and autopsy findings. In conclusion, the administration of CJ-50005 over 81$\mu\textrm{g}$/kg in SD rats and over 27$\mu\textrm{g}$/kg in beagle dogs was proved to be safe, and it is thought that CJ-50005 may not show any toxicity in its clinical use.

• Journal of the Korean Ceramic Society
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• v.54 no.2
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• pp.158-166
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• 2017

For the better success of biomedical implant surgery, we used a modified solution combustion method to synthesize Hydroxyapatite (HA) and Chromium ($Cr^{3+}$) modified Cr-HA with different concentrations of 0.5, 1.0, 1.5, 2.0 and 2.5. The Cr-HA nanopowder was characterized by TGA, XRD, SEM-EDS and TEM. The HA and Cr-HA powders were subjected to in vitro biological studies to determine their biocompatibility and hemocompatibility. The cytotoxicity of HA and Cr-HA were evaluated on Hela (Cervical cancer) cells and L929 (mouse fibroblast) cells by using MTT assay. Hemocompatibility studies demonstrated a noticeable haemolytic ratio below 5%, which confirms that these materials are compatible in nature with human blood. The results of the present work confirm that the synthesised HA and Cr-HA are biocompatible and can be extensively used in the biomedical field to improve overall material biological properties.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.22 no.2
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• pp.70-75
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• 1996

DMHF (2.5-dimethyl-4-hydroxy-3[2H]-furanone), an antioxidative compound from the reaction of L-cysteine/D-glucose scavenged efficiently 1,1-diphenyl-2-picryl hydrazyl free radicals. It exhibited an inhibitory effect on the autoxidation of linolenic acid, and the protective effect against UV cytotoxicity in cultured human fibroblast. In addition, DMHF appeared to prevent the cellular melanogenesis in the cultured murine melanoma cells more effectively than kojic acid, a well known inhibitor of melanogenesis, while the former was not so effective as the latter for the inhibition of the tyrosinase. Considering that cellular melanogenesis is a metabolic process triggered by oxidative stress, it ovas tentatively deduced that the antioxidative property of DMHF might afford the effect against cellular pigmentation by alleviating the causative stress. In toxicological tests such as irritation and sensitization, this compound turned out to be safe. The results of this study suggest that DMHF may be a novel inhibitor of melanogenesis, and that night be useful for application in cosmetics.

• Journal of Haehwa Medicine
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• v.17 no.2
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• pp.101-116
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• 2008

In order to investigate the effect of HGCS on Hypercholesterolemia in rats diagnosed with hypertension, changes of related factors in blood and tissues of animal models were studied. The results are as following: At 250 ${\mu}g/m{\ell}$ or lower doses of HGCS, human fibroblast cells showed a survival rate of more than 90%. HGCS significantly reduced the total cholesterol and LDL cholesterol levels in blood observed in hypercholesterolemia rats. On the other hand, HGCS signicantly increased the blood HDL cholesterol level, compared to that of the control group. HGCS significantly reduced triglycerides caused by hypercholesterolemia. However, glucose level was decreased insignificantly. Significant decrease of hyperoxidative lipid level in tissues due to hypercholesterolemia was observed in HGCS treated group. Significant increase of SOD and catalase activities in tissues due to hypercholesterolemia were observed in HGCS treated group. From the results above, the anti-hypercholesterolemic effect of clinically used HGCS has been experimentally proved. More active prescription of HGCS to hypercholesterolemia and related patients should be available in the future.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2013.10a
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• pp.946-948
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• 2013

Several baculovirus vector systems recombined with coding genes of polyhedron promoter, vesicular stomatitis virus G (VSVG), polyA, cytomegalovirus (CMV) promoter, enhanced green fluorescent protein (EGFP), and protein transduction domain (PTD) were constructed. These recombinant baculovirus vector systems were applied into human foreskin fibroblast cells and compared the effects of gene transfer and gene expression of these recombinant baculovirus vector systems with control vector system. From this study, it showed that these novel recombinant baculovirus vector systems were superior efficacy to control vector system in view of gene transfer and gene expression.