Browse > Article
http://dx.doi.org/10.5657/kfas.2011.0456

Characterization of a Collagenase-1 Inhibitory Peptide Purified from Skate Dipturus chilensis Skin  

Park, Sung-Ha (Department of Marine Biotechnology, Gangneung-Wonju National University)
Lee, Jung-Kwon (Department of Marine Biotechnology, Gangneung-Wonju National University)
Jeon, Joong-Kyun (Department of Marine Biotechnology, Gangneung-Wonju National University)
Byun, Hee-Guk (Department of Marine Biotechnology, Gangneung-Wonju National University)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.44, no.5, 2011 , pp. 456-463 More about this Journal
Abstract
We attempted to isolate a collagenase-1 inhibitory peptide from skate Dipturus chilensis skin protein. The protein from skate skin was digested by various enzymes (alcalase, ${\alpha}$-chymotrypsin, neutrase, papain, pepsin, and trypsin) to produce a collagenase-1 inhibitory peptide. The collagenase-1 inhibitory activity of the peptides obtained was measured by gelatin digestion assay. Among the six hydrolysates, pepsin hydrolysate exhibited the highest collagenase-1 inhibitory activity. The peptide showing strong collagenase-1 inhibitory activity was purified by Sephadex G-25 gel chromatography and HPLC using an octadecylsilyls (ODS) column. The amino acid sequence of purified collagenase-1 inhibitory peptide was identified to be Asn-Leu-Asp-Val -Leu-Glu-Val-Phe (961 Da) by quadrupole time of flight (Q-TOF) and electrospray ionization mass spectrometry (ESI-MS) mass spectroscopy. The $IC_{50}$ value of purified peptide was 87.0 ${\mu}M$. Moreover, the peptide did not exhibit cytotoxic effects on human dermal fibroblast cell lines.
Keywords
Skate skin; Collagenase-1 inhibitory activity; Peptide; Purification;
Citations & Related Records
Times Cited By KSCI : 8  (Citation Analysis)
연도 인용수 순위
1 Lodemel JB, Egge-Jacobsen W, and Olsen RL. 2004. Detection of TIMP-2-like protein in Atlantic cod (Gadus morhua ) muscle using two-dimensional real-time reverse zymography. Comp Biochem Physiol 139, 253-259.   DOI
2 Mosmann T. 1983. Rapid colorimetric assay for the cellualr growth and survival : application to proliferation and cytotoxic assay. J Immunol Methods 65, 55-59.   DOI
3 MOMAF. 2010. Statistic Database for Fisheries Production. Korea National Statistical Office, Daejeon, Korea
4 Tamsyn SAT, Pauline H and Declan PN. 2009. Anticollagenase, anti-elastase and anti-oxidant activities of extracts from 21 plants. BMC Complementary and Alternative Medicine 9:27.   DOI
5 Je JY, Qian ZJ, Byun HG and Kim SK. 2007. Purification and characterization of an antioxidant peptide obtained from tuna backbone protein by enzymatic hydrolysis. Process Biochem 42, 840-846.   DOI
6 Jeong BY, Choi BD and Lee JS. 1998. Proximate Composition, Cholesterol and ${alpha}$-Tocopherol Content in 72 Species of Korean Fish. J Korean Fish Soc 31, 160-167.   과학기술학회마을
7 Kim JM, Jeon SW, Lee WG, Nam HJ, and Kim YB. 2010. Study of preventing methods for skin aging and wrinkles. Korean J. Oriental Physiology & Pathology, 24, 533-542.   과학기술학회마을
8 Kim MM, Ta QV, Mendis E, Rajpakse N, Jung WK, Byun HG, Jeon YJ and Kim SK. 2006. Phlorot annins in Ecklonia cava extract inhibit matrix metalloproteinase activity. Life Sciences 79, 1436-1443.   DOI
9 Kim SK, Yang HP and Lee EH. 1991. The development of a natural seasoning using the enzymatic hydrolysate of fish skin. Korean J Biotechnol Bioeng 6, 327-366   과학기술학회마을
10 Kong SC, Kim JA, Ahn B, Byun HG and Kim SK. 2010. Carboxymethylations of chitosan and chitin inhibit MMP expression and ROS scavenging in human fibrosarcoma cells. Process Biochem 45, 179-186.   DOI
11 Kwon MC, Kim HC, Kim SH, Syed AQ, Hwang BY and Lee HY. 2007. Anti-wrinkle activity of low molecular weight peptides derived from the collagen isolated from asterias amurensis. Korean J Food Sci Technol 39, 625-629.   과학기술학회마을
12 Choi YR., Kim SK, Park PJ, Choi JH and Moon SH. 2000. Purification and characterization of antioxidative peptides from enzymatic hydrolysate of Cod teiset protein. J Korean Fish Soc 33, 198-204.   과학기술학회마을
13 Lee MJ and Jeong NH. 2009. Preparation and availability analysis of collagen peptides obtained in fish scale. J Korean Oil Chemists Soc 26, 457-466.   과학기술학회마을
14 Cho SY, Kim JS and Kim JG. 1997. Screening for the raw material of gelatin from the skins of some pelagic fishes and squid. J Korean Fish Soc 30, 55-61.   과학기술학회마을
15 Choi JH. 2003. Isolation and Purification of chondroitin sulfate from skate cartilage. Master. Thesis, Pukyong National University, Busan, Korea, 1-65.
16 Do JR., Heo IS, Jo JH, Kim DS, Kim HK, Kim SS and Han CK. 2006. Effect of antihyperteinsive peptides originated from various marine proteins on ACE inhibitory activity and systolic blood pressure in spontaneously hypertensive Rats. Korean J Food Sci Technol 38, 567-570.
17 Eun JB, Cho SH, Jahncke ML and Chin GB. 2006. The of processing conditions on the properties of gelatin from skate(Raja kenojei ) skin. Food Hydrocolloids 20, 810-816.   DOI
18 Gomez-Guillen MC, Yurnay J, Fernandez-Diaz MD, Ulmo N, Lizarbe MA and Montero P. 2002. Structural and physical properties of gelatin extracted from different marine species: a comparative study. Food Hydrocolloid 16, 25-34.   DOI
19 Hideaki N, Robert V and Gullian M. 2006. Structure and function of matrix metalloprotease and TIMPs. Cardiovasc Res 69, 562-573.   DOI
20 Hwang JH, Mizuta S, Yokoyama Y and Yoshinaka R. 2007. Purification and characterization of molecular species of collagen in the skin of skate (Raja kenojei ). Food Chem 100, 921-925.   DOI
21 Jia J, Zhou Y, Lu J, Chen A, Li Y and Zheng G. 2010. Enzymatic hydrolysis of Alaska pollack (Theragra chalcogramma ) skin and antioxidant activity of the resulting hydrolysate. J Sci Food Agric 90, 635-640.
22 Borkakoti N. 2004. Matrix metalloprotease inhibitors: design from structure. Biochemical Society Transactions 32. part 1.
23 AOAC. 1995. Official methods of analysis. 16th ed. Association of Official Analytical Chemists. Washington, DC, U.S.A., 69-74.
24 Ahn YS, Lee WW, Lee SH, Ahn G, Ko CI, Oh CK, Oh MC, Kim DW, Jeon YJ and Kim SH. 2009. Processing and Biological Activity of Gelatin Hydrolysate from Branchiostegus japonicus scales. Korean J Fish Aquat. Sci 42, 417-425.   과학기술학회마을   DOI