Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
권호 표지
DOI QR코드

DOI QR Code

Characterization of a Collagenase-1 Inhibitory Peptide Purified from Skate Dipturus chilensis Skin

홍어류(Dipturus chilensis) 껍질로부터 분리 정제된 collagenase-1 저해 펩타이드의 특성

  • Park, Sung-Ha (Department of Marine Biotechnology, Gangneung-Wonju National University) ;
  • Lee, Jung-Kwon (Department of Marine Biotechnology, Gangneung-Wonju National University) ;
  • Jeon, Joong-Kyun (Department of Marine Biotechnology, Gangneung-Wonju National University) ;
  • Byun, Hee-Guk (Department of Marine Biotechnology, Gangneung-Wonju National University)
  • 박성하 (강릉원주대학교 해양생물공학과) ;
  • 이정권 (강릉원주대학교 해양생물공학과) ;
  • 전중균 (강릉원주대학교 해양생물공학과) ;
  • 변희국 (강릉원주대학교 해양생물공학과)
  • Received : 2011.06.08
  • Accepted : 2011.10.07
  • Published : 2011.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

We attempted to isolate a collagenase-1 inhibitory peptide from skate Dipturus chilensis skin protein. The protein from skate skin was digested by various enzymes (alcalase, ${\alpha}$-chymotrypsin, neutrase, papain, pepsin, and trypsin) to produce a collagenase-1 inhibitory peptide. The collagenase-1 inhibitory activity of the peptides obtained was measured by gelatin digestion assay. Among the six hydrolysates, pepsin hydrolysate exhibited the highest collagenase-1 inhibitory activity. The peptide showing strong collagenase-1 inhibitory activity was purified by Sephadex G-25 gel chromatography and HPLC using an octadecylsilyls (ODS) column. The amino acid sequence of purified collagenase-1 inhibitory peptide was identified to be Asn-Leu-Asp-Val -Leu-Glu-Val-Phe (961 Da) by quadrupole time of flight (Q-TOF) and electrospray ionization mass spectrometry (ESI-MS) mass spectroscopy. The $IC_{50}$ value of purified peptide was 87.0 ${\mu}M$. Moreover, the peptide did not exhibit cytotoxic effects on human dermal fibroblast cell lines.

Keywords

References

  1. AOAC. 1995. Official methods of analysis. 16th ed. Association of Official Analytical Chemists. Washington, DC, U.S.A., 69-74.
  2. Ahn YS, Lee WW, Lee SH, Ahn G, Ko CI, Oh CK, Oh MC, Kim DW, Jeon YJ and Kim SH. 2009. Processing and Biological Activity of Gelatin Hydrolysate from Branchiostegus japonicus scales. Korean J Fish Aquat. Sci 42, 417-425. https://doi.org/10.5657/kfas.2009.42.5.417
  3. Borkakoti N. 2004. Matrix metalloprotease inhibitors: design from structure. Biochemical Society Transactions 32. part 1.
  4. Cho SY, Kim JS and Kim JG. 1997. Screening for the raw material of gelatin from the skins of some pelagic fishes and squid. J Korean Fish Soc 30, 55-61.
  5. Choi JH. 2003. Isolation and Purification of chondroitin sulfate from skate cartilage. Master. Thesis, Pukyong National University, Busan, Korea, 1-65.
  6. Choi YR., Kim SK, Park PJ, Choi JH and Moon SH. 2000. Purification and characterization of antioxidative peptides from enzymatic hydrolysate of Cod teiset protein. J Korean Fish Soc 33, 198-204.
  7. Do JR., Heo IS, Jo JH, Kim DS, Kim HK, Kim SS and Han CK. 2006. Effect of antihyperteinsive peptides originated from various marine proteins on ACE inhibitory activity and systolic blood pressure in spontaneously hypertensive Rats. Korean J Food Sci Technol 38, 567-570.
  8. Eun JB, Cho SH, Jahncke ML and Chin GB. 2006. The of processing conditions on the properties of gelatin from skate(Raja kenojei ) skin. Food Hydrocolloids 20, 810-816. https://doi.org/10.1016/j.foodhyd.2005.08.002
  9. Gomez-Guillen MC, Yurnay J, Fernandez-Diaz MD, Ulmo N, Lizarbe MA and Montero P. 2002. Structural and physical properties of gelatin extracted from different marine species: a comparative study. Food Hydrocolloid 16, 25-34. https://doi.org/10.1016/S0268-005X(01)00035-2
  10. Hideaki N, Robert V and Gullian M. 2006. Structure and function of matrix metalloprotease and TIMPs. Cardiovasc Res 69, 562-573. https://doi.org/10.1016/j.cardiores.2005.12.002
  11. Hwang JH, Mizuta S, Yokoyama Y and Yoshinaka R. 2007. Purification and characterization of molecular species of collagen in the skin of skate (Raja kenojei ). Food Chem 100, 921-925. https://doi.org/10.1016/j.foodchem.2005.10.046
  12. Jia J, Zhou Y, Lu J, Chen A, Li Y and Zheng G. 2010. Enzymatic hydrolysis of Alaska pollack (Theragra chalcogramma ) skin and antioxidant activity of the resulting hydrolysate. J Sci Food Agric 90, 635-640.
  13. Je JY, Qian ZJ, Byun HG and Kim SK. 2007. Purification and characterization of an antioxidant peptide obtained from tuna backbone protein by enzymatic hydrolysis. Process Biochem 42, 840-846. https://doi.org/10.1016/j.procbio.2007.02.006
  14. Jeong BY, Choi BD and Lee JS. 1998. Proximate Composition, Cholesterol and ${alpha}$-Tocopherol Content in 72 Species of Korean Fish. J Korean Fish Soc 31, 160-167.
  15. Kim JM, Jeon SW, Lee WG, Nam HJ, and Kim YB. 2010. Study of preventing methods for skin aging and wrinkles. Korean J. Oriental Physiology & Pathology, 24, 533-542.
  16. Kim MM, Ta QV, Mendis E, Rajpakse N, Jung WK, Byun HG, Jeon YJ and Kim SK. 2006. Phlorot annins in Ecklonia cava extract inhibit matrix metalloproteinase activity. Life Sciences 79, 1436-1443. https://doi.org/10.1016/j.lfs.2006.04.022
  17. Kim SK, Yang HP and Lee EH. 1991. The development of a natural seasoning using the enzymatic hydrolysate of fish skin. Korean J Biotechnol Bioeng 6, 327-366
  18. Kong SC, Kim JA, Ahn B, Byun HG and Kim SK. 2010. Carboxymethylations of chitosan and chitin inhibit MMP expression and ROS scavenging in human fibrosarcoma cells. Process Biochem 45, 179-186. https://doi.org/10.1016/j.procbio.2009.09.004
  19. Kwon MC, Kim HC, Kim SH, Syed AQ, Hwang BY and Lee HY. 2007. Anti-wrinkle activity of low molecular weight peptides derived from the collagen isolated from asterias amurensis. Korean J Food Sci Technol 39, 625-629.
  20. Lee MJ and Jeong NH. 2009. Preparation and availability analysis of collagen peptides obtained in fish scale. J Korean Oil Chemists Soc 26, 457-466.
  21. Lodemel JB, Egge-Jacobsen W, and Olsen RL. 2004. Detection of TIMP-2-like protein in Atlantic cod (Gadus morhua ) muscle using two-dimensional real-time reverse zymography. Comp Biochem Physiol 139, 253-259. https://doi.org/10.1016/j.cbpc.2004.08.004
  22. Mosmann T. 1983. Rapid colorimetric assay for the cellualr growth and survival : application to proliferation and cytotoxic assay. J Immunol Methods 65, 55-59. https://doi.org/10.1016/0022-1759(83)90303-4
  23. MOMAF. 2010. Statistic Database for Fisheries Production. Korea National Statistical Office, Daejeon, Korea
  24. Tamsyn SAT, Pauline H and Declan PN. 2009. Anticollagenase, anti-elastase and anti-oxidant activities of extracts from 21 plants. BMC Complementary and Alternative Medicine 9:27. https://doi.org/10.1186/1472-6882-9-27

Cited by

  1. A Study on Development of Protein Materials using Dead Flatfish from Fish Farms(1) -Antioxidant Functional Properties- vol.22, pp.11, 2013, https://doi.org/10.5322/JESI.2013.22.11.1465
  2. Extraction Characteristics, Antioxidative Effect and Preparation of Collagen Gel of Skate Skin Extracts vol.13, pp.11, 2012, https://doi.org/10.5762/KAIS.2012.13.11.5637
  3. Development of Bioactive Substances from Fishery Processing by-products in Jeju vol.6, pp.2, 2014, https://doi.org/10.15433/ksmb.2014.6.2.062
  4. Convergence Study on Preparation of Anti-aging Peptides from Fish Collagen Hydrolysates vol.11, pp.5, 2020, https://doi.org/10.15207/jkcs.2020.11.5.061
  5. Peptides and Peptidomimetics as Inhibitors of Enzymes Involved in Fibrillar Collagen Degradation vol.14, pp.12, 2011, https://doi.org/10.3390/ma14123217