• 한국농공학회논문집
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• 제63권1호
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• pp.75-87
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• 2021

Air quality related to particulate matters and ammonia is being come to the fore as the national concern in Korea. CAPSS (Clean Air Policy Support System) provides emission coefficients of these kinds of particulate and gaseous matters in the fields of livestock; however reliability issues are consistently mentioned. Evaluation of emission rates of PM2.5 and NH3 of the country is very important, but only few studies are available as the background related to observation of the concentration of the particulate matter and ammonia, especially within livestock house in Korea. In this paper, long-term measurement of PM10, PM2.5, and ammonia within the mechanically ventilated broiler house were carried out to introduce backgrounds of generation and emission of the particulate matters and ammonia. Measurement results were analyzed according to seasonal changes, age of broilers(weeks) and measurement locations. Concentration of inhalable and respirable dust were also evaluated in terms of occupational respiratory health according to increase in broiler's activity. From the results of this study, identification of the generation mechanisms of the particulate and gaseous matters, and evaluation of the emission rate of these in the broiler house will be carried out.

• 한국안전학회지
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• 제12권1호
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• pp.108-112
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• 1997

Recently, the use of Dwelling House has been expanded due to the rapid urbainzation and congestion of population in City. Indoor Air Quality in apartment houses was investigated by measuring pollutants such as $CO_2$ gas, dust concentration. In this study, the mesurements of indoor air environment were performed in Dwelling house in Seoul area to investigate indoor air environment and the relation of air contaminative and the response level. Indoor air contamination and living environment of the occupants have been compared & analyzed with practical investigation of IAQ in Seoul, Korea and Tokyo, Japan.

• 한국컴퓨터정보학회논문지
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• 제24권8호
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• pp.95-103
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• 2019

According to the Statistics Korea in 2017, the 10 leading causes of death contain a cardiac disorder disease, self-injury. In terms of these diseases, urgent assistance is highly required when people do not move for certain period of time. We propose an unusual event detection algorithm to identify abnormal user behaviors using dust, vision and activity sensors in their houses. Vision sensors can detect personalized activity behaviors within the CCTV range in the house in their lives. The pattern algorithm using the dust sensors classifies user movements or dust-generated daily behaviors in indoor areas. The accelerometer sensor in the smartphone is suitable to identify activity behaviors of the mobile users. We evaluated the proposed pattern algorithms and the fusion method in the scenarios.

• 한국농공학회논문집
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• 제59권4호
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• pp.75-83
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• 2017

Many parts of problems in livestock industry today are associated with organic dust. Endotoxin and toxic gasses on the surface of dust and dust itself can cause aesthetic displeasure and respiratory disease. It also reduces livestock productivity by suppressing immunity of animals and carrying microbes causing animal disease. However, dust level of cattle farm was rarely reported in Korea, and regulation for cattle farm worker does not exist. In this paper, dust concentration and environmental condition were regularly monitored in a commercial Korean native cattle farm. The measurement was conducted according to location and working activities. From the measurement, distribution of dust concentration was affected by wind environment, as the result of natural ventilation. TMR mixer was a major source of dust in target cattle house. The maximum inhalable dust concentration was 637.8 times higher than exposure limit as feed dropped into the TMR mixer. It was expected that dust generation could be affected by particle size and drop height of feed. This study suggests potential risk of dust in cattle farm, and necessity for latter study. Effect of aerodynamic condition and TMR processing should be investigated for dust reduction study.

• 한국농공학회논문집
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• 제59권3호
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• pp.71-81
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• 2017

Organic dust generated inside livestock facilities includes toxic organic matters such as bacteria and endotoxin. Dust can cause respiratory disease for worker and livestock, and consequently, degradation of welfare and productivity. Influence of dust on livestock workers has been studied since the 1970s. However, exposure limit for cattle farmer has not been established, unlike exposure limit for pig and poultry farmer. Furthermore, study on air quality inside livestock facility, especially inside dairy farm has been rarely conducted in Korea. In this study, dust concentration of TSP, PM10, inhalable and respirable dust has been monitored in the commercial dairy house according to location and working activities. Bedding material inside the stall was one of the major sources of dust. The amount of dust was related to water content level of the bedding material. Dust concentration was relatively high in leeward location, and the highest concentration was measured during TMR mixing process. The maximum value of inhalable dust concentration was 29.1 times higher than the reference value as fine particles drop to the TMR mixer. Dust generated by TMR mixing was presumed to decrease by adjusting moisture and drop height of feed.

• 대한의생명과학회지
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• 제21권2호
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• pp.122-125
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• 2015

House dust mite (HDM) as a major allergen and damage-associated molecular pattern (DAMP) such as S100A8 and S100A9 trigger the pathogenesis and severity of allergic disease such as asthma. Regulation of neutrophil apoptosis is an important immune response and its dysregulation is involved in pathogenesis of allergic diseases. In this study, we examined the effects of HDM, S100A8 and S100A9 on spontaneous apoptosis of normal neutrophils. We considered the importance of the difference between in vitro and in vivo results and developed a new in vitro system consisting of a combination of immune cells and T helper (Th) cytokines. Extract of Dermatophagoides pteronyssinus (DP), S100A8, and S100A9 inhibited neutrophil apoptosis in culture of neutrophils alone without other leukocytes. DP and S100A8 more strongly suppressed neutrophil apoptosis in combinations of neutrophils, eosinophils, lymphocytes or monocytes than in a culture of neutrophils alone. Anti-apoptotic effect of S100A9 in the mixture of immune cells was similar to that in neutrophils. DP, S100A8, and S100A9 blocked neutrophil apoptosis, regardless of pretreatment with a T helper (Th) 1 cytokine (IFN-$\gamma$), Th2 cytokines (IL-4 and IL-10), a Th9 cytokine (IL-9), a Th17 cytokine (IL-17), a Treg-producing cytokine (TGF-$\beta$). These findings may enable elucidation of allergy pathogenesis due to HDM and DAMP.

• International Journal of Oral Biology
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• 제40권1호
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• pp.11-17
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• 2015

The gingival epithelium of the oral cavity is constantly exposed to exogenous stimuli such as bacterial toxins, allergens, and thermal changes. These exogenous stimuli are resisted by innate host defense in gingival epithelial cells. However, it is unclear exactly how the exogenous stimuli affect detrimentally on the human gingival epithelial cells. Here, we investigated whether the allergen, such as house dust mite (HDM) extract, is linked to $Ca^{2+}$ signaling and proinflammatory cytokine expression in primary cultured human gingival epithelial cells. HDM extract induced an increase in intracellular $Ca^{2+}$ concentration ($[Ca^{2+}]_i$) in a dose-dependent manner. Extracellular $Ca^{2+}$ depletion did not affected on the HDM extract-induced increase in $[Ca^{2+}]_i$. The HDM extract-induced increase in $[Ca^{2+}]_i$ was abolished by the treatment with U73122 and 2-APB, which are inhibitors of phospholipase C (PLC) and inositol 1,4,5-trisphosphate ($IP_3$) receptor. Moreover, HDM extract induced the mRNA expression of pro-inflammatory cytokine, interleukin (IL)-8. These results suggest that HDM extract triggers $PLC/IP_3$-dependent $Ca^{2+}$ signaling and IL-8 mRNA expression in primary cultured human gingival epithelial cells.

• 대한의생명과학회지
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• 제20권1호
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• pp.39-42
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• 2014

Asthma is an allergic inflammation and house dust mite (HDM) is a major allergen to induce asthma pathogenesis. Regulation of eosinophil apoptosis is an essential immune process and its dysregulation is implicated in asthma. In the present study, we examined the effects of HDM on spontaneous apoptosis of asthmatic eosinophils and on cytokine secretion in eosinophils of normal subjects including non-atopic and atopic normal. Extract of Dermatophagoides pteronissinus (DP) inhibited eosinophil apoptosis in a time-dependent manner. DP increased the secretion of G-CSF, GM-SCF, and IL-4, which is involved in suppression of eosinophil apoptosis, but IL-5 expression was not altered after DP stimulation. DP also elevated the release of IL-6, IL-8, tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) and CCL2, which are anti-apoptotic or survival factors. The secretion of G-CSF, GM-CSF, IL-6, IL-8, and TNF-${\alpha}$ due to DP is higher in atopic normal than that in non-atopic normal. In conclusion, DP increases the survival of eosinophils and its mechanism may be associated with cytokine release. These findings may enable elucidation of asthma pathogenesis induced by HDM.

• 대한한방소아과학회지
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• 제30권4호
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• pp.77-86
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• 2016

Objectives Hataedock (HTD) is an oral Korean herbal medical oral treatment that removes fetal toxic heat and meconium from new born babies. The purpose of this study is to evaluate whether Hataedock treatment of Duchi extracts has anti-inflammation effects in atopic dermatitis-like skin lesions in House Dust Mite-Induced NC/Nga Mice. Methods The mice were divided into 3 groups (n=10 per group) as follows: the control group (Ctrl group), AD-induced group (AE group), AD-induced with HTD treatment group (DT group). 3-week-old NC/Nga mice were introduced to Hataedock treatment, made of Duchi extract. After 4 weeks, House Dust Mite-Induced application was used six times per week for 3 weeks to induce the first atopic dermatitis, and second AD in 7 weeks after. To examine skin injuries and anti-inflammatory effect, PKC, MMP-9, iNOS immunohistochemistry were used. Results The alleviate effect of the skin damage and angiogenesis was observed in DT group. The damage of stratum corneum, hyperplasia, edema, infiltration of lymphocytes and distribution of capillary were decreased in DT group. Also, the study results suggested that Hataedock treatment made of Duchi extracts in DT group remarkably decreased skin damages by 51% (p < 0.001), as well as PKC by 91%, MMP-9 by 48% (p < 0.001), iNOS by 51% (p < 0.001). Conclusions Based on the study results, we observed that Hataedock treatment of Duchi extracts alleviates AD by diminishing various inflammatory cytokines, initial steps of AD development, in the skin lesions. Potential applications for prevention and treatment of atopic dermatitis are expected.

• Parasites, Hosts and Diseases
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• 제35권3호
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• pp.149-154
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• 1997

저장 곡류 해충으로 알려져 있는 긴털가루진드기(rophnwpkescentios)가 우리 나라 가옥 내 집먼지 서식 진드기류 중 제 3의 우점종으로 밝혀짐에 따라 알레르기성 질환을 야기하는 원인 항원으로 작용할 가능성이 높아져 연구의 대상이 되고 있다. 본 연구에서는 진단, 치료 및 면역학 적 연구에 필요한 다량의 항원을 공급하기 위하여 대량 사육방법의 개발을 시도하여 다음과 같은 결과를 얻었다. 긴털가루진드기를 대량 사육하는데 가장 적합한 사육배지로는 실험동물사료 분말 로 사육 10주 후 1,251.5배의 개체 증가를 보였고 효소분말과 실험동물 사료분말의 혼합배지 (1 : 1)에서도 사육 10주 후 1.203.1배의 개체 증가를 보여 거의 같은 성적을 보였다. 사육조건으 로 가장 적합한 온도와 상대습도는 $25^{\circ}C$. 64% RH로서 9주 후 960배로 가장 높은 증식결과를 보였고, $^25{\circ}C$와 75% RH에서도 935.4배의 개체 증가로 거의 동일한 결과를 나타냈다 사육용기 표면적을 $79{\;}cm^2,{\;}154{\;}cm^2{\;}및{\;}227{\;}cm^2$로 사육 비교한 결과 각각 860.8배. 924.6배 및 1,250.8 배의 개체 증가를 보여 현저한 차이는 보이지 않았다.