• Journal of Microbiology and Biotechnology
• /
• v.25 no.8
• /
• pp.1349-1360
• /
• 2015

The rhizospheric zone abutting plant roots usually clutches a wealth of microbes. In the recent past, enormous genetic resources have been excavated with potential applications in host plant interaction and ancillary aspects. Two Pseudomonas strains were isolated and identified through 16S rRNA and rpoD sequence analyses as P. fluorescens QAU67 and P. putida QAU90. Initial biochemical characterization and their root-colonizing traits indicated their potential role in plant growth promotion. Such aerobic systems, involved in gluconic acid production and phosphate solubilization, essentially require the pyrroloquinoline quinine (PQQ)-dependent glucose dehydrogenase (GDH) in the genome. The PCR screening and amplification of GDH and PQQ and subsequent induction of mutagenesis characterized their possible role as antioxidants as well as in growth promotion, as probed in vitro in lettuce and in vivo in rice, bean, and tomato plants. The results showed significant differences (p ≤ 0.05) in parameters of plant height, fresh weight, and dry weight, etc., deciphering a clear and in fact complementary role of GDH and PQQ in plant growth promotion. Our study not only provides direct evidence of the in vivo role of GDH and PQQ in host plants but also reveals their functional inadequacy in the event of mutation at either of these loci.

• Pediatric Gastroenterology, Hepatology & Nutrition
• /
• v.19 no.1
• /
• pp.29-37
• /
• 2016

Purpose: The aim of our study was to establish a safe and convenient diagnostic method for acute gastrointestinal (GI) graft-versus-host disease (GVHD) in children by determining the sensitivity and negative predictive values of upper and lower endoscopic biopsies for children suspected of GI GVHD. Methods: Patients suspected of GI GVHD who received endoscopic evaluation within 100 days after stem cell transplantation and endoscopies between January 2012 and March 2014 in Seoul National University Children's Hospital were included in our study. Results: Fifteen patients with a total of 20 endoscopic procedures were included in our study. Sensitivity at the esophagus, stomach, and duodenum were 22.2%, 30.0%, and 80.0%, respectively. Negative predictive values at the esophagus, stomach, and duodenum were 22.2%, 30.0%, and 60.0%, respectively. Overall sensitivity and negative predictive values of upper endoscopic biopsy for GVHD were 77.8% and 50.0%, respectively. Overall sensitivity and negative predictive values of lower endoscopic biopsy for GVHD were 88.9% and 66.7%, respectively. Conclusion: We recommend flexible sigmoidoscopy as a safe and accurate diagnostic tool for GVHD, similar to other studies reported previously. However, if there is no evidence of GVHD on sigmoidoscopy with high index of suspicion of GI bleeding, full colonoscopy and upper endoscopy should be considered.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.5 no.5
• /
• pp.447-453
• /
• 2004

In this paper, we propose a new system to block harmful Internet information based on IP spoofing. The proposed system is located on a organization's internal network and monitors all outgoing traffic and lets all this traffic go outside. Once the proposed system detects a host's access to a harmful site, it sends the host a pseudo RST packet that pretends to be the response from the harmful site, and prevents the connection between the host and the harmful site. The proposed software system is installed on only a server, and need not be installed on user hosts at all. Thus we can maintain and upgrade the blocking system easily. The performance evaluation of the proposed system shows that it effectively blocks the access to the harmful sites. Since the proposed system is based on IP spoofing, it can be used badly as a hacking tool. Finally we propose some methods to eliminate this possibility.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.22 no.12
• /
• pp.1667-1674
• /
• 2018

Recently, speeding up real time calculation using the specialized hardware accelerator is often used in the various engineering and science area, and the accelerators are required to include PCI express interconnection between FPGA and a host computer. The implementation of the high speed PCIe for the multi-giga bytes per second transmission is one of the most difficult issue in the development of the accelerators. There are several commercialized IP solutions and research results in the literature, but these solutions are required extra cost and design period to analyze the detailed implementation method. For the hardware accelerator on Xilinx FPGA, utilizing Xilinx's XDMA PCIe IP, which is provided without extra charge, can be the best solution in terms of the development period and cost. Consequently, this paper presents the evaluation system on Zynq-7000 FPGA and Windows 10 host computer, and analyze the performance of the PCIe IP with various configuration parameters.

• Journal of Food Hygiene and Safety
• /
• v.37 no.5
• /
• pp.306-309
• /
• 2022

Probiotics are live microorganisms that confer health benefits onto the host when administered at adequate doses. Most widely used probiotics, such as lactobacilli and bifidobacteria, are known to be elements of healthy gut microflora and hence are not considered a threat to the host. However, probiotics may pose a risk in certain populations with compromised immune systems or defects in gut barrier functions. Herein, we evaluated the safety of Bifidobacterium breve BB077, according to the safety evaluation guidelines for probiotics produced by the National Institute of Food and Drug Safety Evaluation (NIFDS). The results show that B. breve BB077 is both non-hemolytic and non-cytolytic. In contrast, B. breve BB077 exhibited higher streptomycin and tetracycline resistance than the suggested NIFDS standard cut-off values. Hence, a genetic analysis of the streptomycin and tetracycline resistance genes was performed to determine the origin of antimicrobial resistance. Streptomycin and tetracycline resistance was shown have arisen from chromosomal mutations and considered intrinsic to the taxonomic group. In conclusion, the B. breve BB077 strain might be safe for human consumption.

• 한국균학회소식:학술대회논문집
• /
• 2015.05a
• /
• pp.20-20
• /
• 2015

Endophytic fungi are microorganisms inhabiting living plant tissues without causing apparent harm to the host. They are drawing increasing attention due to their ability to produce various bioactive compounds as well as their effects on host growth and resistance to biotic and abiotic stresses. As a first step to assess biodiversity of plant associated fungi in Korea and the following evaluation on diverse biological activities, we are collecting endophytic fungi from plant in wild followed by systematic long-term storage in liquid nitrogen. Molecular identification using ITS sequences was also incorporated for pure culture by hyphal tip isolation. As of April 2015, about 1,400 fungal strains had been isolated from about 170 plant taxa. Fungal isolates belonging to Pleosporales, Diaporthales, Glomerellales, Hypocreales, and Xylariales were the most abundant. These collections are being used for several complementary researches, including screening of isolates with novel bioactive compounds or conferring drought stress resistance, phylogenetic and genomic study. Genome sequencing was performed for 3 isolates, one Xylaria sp. strain JS573 producing griseofulvin, an antifungal compound, and two Fusarium spp. strains JS626 and JS1030, which are assumed to be new species found in Korea. More detailed analysis on these genomes will be presented. These collections and genome informations will serve as invaluable resources for identifying novel bioactive materials in addition to expand our knowledge on fungal biodiversity.

• Proceedings of the IEEK Conference
• /
• 2005.11a
• /
• pp.1035-1038
• /
• 2005

This paper describes the designs and the implementations of two H/W IPSec Systems, look-aside and inline, on TOE (Transport Offloading Engine). These systems aim for guaranteeing the security of datagram networks while preserving the bandwidth of gigabit networks. The TOE offloads a host CPU from network burdens, so that it makes the gigabit wire speed possible, and then deeper level security architecture of the IPSec guarantees the security of gigabit service network dominated by datagram packets. The focus of this paper is to minimize the TOE's performance degradation caused by the computation-oriented IPSec. The look-aside IPSec system provides a significant improvement in the CPU offload of the IPSec cryptography loads. However, the inline system completely offloads the host CPU from whole IPSec loads, providing significant additional cost saving compared to the look-aside system. In this paper, the implementations of TOE cards including commercial IPSec processors are presented. As the result of performance evaluation with the protocol analyzer, we can get the fact that the inline IPSec system is 8 times faster than the S/W system and 2 times faster than the look-aside system.

• Journal of the Korean Society of Clothing and Textiles
• /
• v.31 no.9_10
• /
• pp.1333-1341
• /
• 2007

The purposes of the present study were to investigate the impact of a apparel brand and an ingredient brand on evaluation(perceived quality, favorable attitude, purchase intention) of apparel products, and to examine the differences in evaluations toward apparel products allied with ingredient brands between groups divided by product knowledge and leisure involvement. The subjects were 235 men and women aged 20 to 40. Two-by-two factorial design were employed. A series of ANOVA were used to determine if significant differences existed in evaluations of apparel products. There was a significant interaction effect of an apparel brand and an ingredient brand on evaluations of apparel products. Empirical results showed that ingredient brands would improve the consumers' perceived quality, favorable attitude and purchase intention toward apparel products. In addition, the results indicated that the respondents that had high leisure involvement and high product knowledge were more likely to evaluate apparel products based on the ingredient brand of the products. However, the respondents that had low leisure involvement and product knowledge were more likely to evaluate apparel products based on the host brand of the products.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.14 no.6
• /
• pp.1382-1390
• /
• 2010

A multicast session protocol in wireless networks reduces the session delay of multicast delivery caused by moving of mobile host. A hand-off scheme, called MSDR (multicast session delay reduction), in mobile networks is proposed. MSDR protocol that minimizes the delay of a session re-establishment uses the basic unicast routing function of the IETF mobile IP and the DFA (designated foreign agent) to provide multicast services for mobile hosts. Proposed MSDR protocol allows the mobile hosts to continuously receive packets when they move across the basic sets during hand-off. Discrete-event simulation carried out for performance evaluation of MSDR protocol, and simulation results indicated that our scheme can offer a better performance of multicast session delay reduction in terms of signalling cost than that of IETF.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.51 no.1
• /
• pp.23-30
• /
• 2018

Kudoa septempunctata, a myxosporean parasite that infects olive flounder Paralichthys olivaceus is known to cause Kudoa food poisoning. Entire trunk muscle (ETM) is used for diagnosis of the parasite in fish and this method demands sacrifice of the host, causing a loss of commercial value. We developed a non-destructive method that uses a plastic syringe-style implanter to draw the sample, called the part-point muscle (PPM) sampling technique. We validated the PPM method in fish infected with K. septempunctata at the level detectable by the ETM method. We confirmed that the PPM method is equally sensitive in comparison to the ETM method for diagnosing K. septempunctata spores in olive flounder muscle. Our study also confirmed that the parasite is uniformly distributed in the dorsal muscle of infected fish. Over a period of 1 month, we observed no mortality of the host fish used for sampling by the PPM method. Thus, our studies demonstrate that the PPM sampling technique is an efficient, non-destructive method for diagnosing K. septempunctata in olive flounder.