Browse > Article
http://dx.doi.org/10.5657/KFAS.2018.0023

Evaluation of a Non-destructive Diagnostic Test for Kudoa septempunctata in Farmed Olive Flounder Paralichthys olivaceus  

Song, Jun-Young (Southest Sea Fisheries Research Institute)
Jung, Sung Hee (Pathology Division, National Institute of Fisheries Science)
Choi, Hye-Sung (Aquaculture Industry Division, South Sea Fisheries Research Institute)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.51, no.1, 2018 , pp. 23-30 More about this Journal
Abstract
Kudoa septempunctata, a myxosporean parasite that infects olive flounder Paralichthys olivaceus is known to cause Kudoa food poisoning. Entire trunk muscle (ETM) is used for diagnosis of the parasite in fish and this method demands sacrifice of the host, causing a loss of commercial value. We developed a non-destructive method that uses a plastic syringe-style implanter to draw the sample, called the part-point muscle (PPM) sampling technique. We validated the PPM method in fish infected with K. septempunctata at the level detectable by the ETM method. We confirmed that the PPM method is equally sensitive in comparison to the ETM method for diagnosing K. septempunctata spores in olive flounder muscle. Our study also confirmed that the parasite is uniformly distributed in the dorsal muscle of infected fish. Over a period of 1 month, we observed no mortality of the host fish used for sampling by the PPM method. Thus, our studies demonstrate that the PPM sampling technique is an efficient, non-destructive method for diagnosing K. septempunctata in olive flounder.
Keywords
Kudoa septempunctata; Olive flounder; Paralichthys olivaceus; Diagnostic test;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Iijim Y, Nakanishi N, Furusawa H, Ohnishi T and Sugita-Konishi Y. 2012. Inter-laboratory validation and applications of quantitative real-time PCR for the detection of Kudoa septempunctata in olive flounder (Paralichthys olivaceus). Jpn J Infect Dis 65, 436-438. https://doi.org/10.7883/yoken.65.436.   DOI
2 Harada T, Kawai T, Jinnai M, Ohnishi T, Sugita-Konishi Y and Kumeda Y. 2012a. Detection of Kudoa septempunctata 18S ribosomal DNA in patient fecal samples from novel foodborne outbreaks caused by consumption of raw olive flounder (Paralichthys olivaceus). J Clin Microbiol 50, 2964-2968. https://doi.org/10.1128/jcm.01218-12.   DOI
3 Harada T, Kawai T, Sato H, Yokoyama H and Kumeda Y. 2012b. Development of a quantitative polymerase chain reaction assay for detection of Kudoa septempunctata in olive flounder (Paralichthys olivaceus). Int J Food Microbiol 156, 161-167. https://doi.org/10.1016/j.ijfoodmicro.2012.03.018.   DOI
4 Kudo G, Barnett H and Nelson R. 1987. Factors affecting cooked texture quality of Pacific whiting, Merluccius productus, fillets with particular emphasis on the effects of infection by the myxosporeans Kudoa paniformis and K. thyrsitis. Fish Bull 85, 745-756. https://spo.nmfs.noaa.gov/sites/default/files/pdf-content/1987/854/kudo.pdf.
5 MAFF (Ministry of Agriculture, Forestry and Fisheries). 2012. Retrieved from http://www.jfa.maff.go.jp/test/saibai/pdf/kudoa_notice_03.pdf on Sep 17, 2017.
6 Matsukane Y, Sato H, Tanaka S, Kamata Y and Sugita-Konishi Y. 2010. Kudoa septempunctata n. sp. (Myxosporea: Multivalvulida) from an aquacultured olive flounder (Paralichthys olivaceus) imported from Korea. Parasitol Res 107, 865-872. https://doi.org/10.1007/s00436-010-1941-8.   DOI
7 Song JY, Choi JH, Choi HS, Jung SH and Park MA. 2013. Monitoring of Kudoa septempunctata in cultured olive flounder during 2012. J Fish Pathol 26, 129-137. https://doi.org/10.7847/jfp.2013.26.3.129.   DOI
8 Kawai T, Sekizuka T, Yahata Y, Kuroda M, Kumeda Y, Iijima Y, Kamata Y, Sugita-Konishi Y and Ohnishi T. 2012. Identification of Kudoa septempunctata as the causative agent of novel food poisoning outbreaks in Japan by consumption of Paralichthys olivaceus in raw fish. Clin Infect Dis 54, 1046-1052. https://doi.org/10.1093/cid/cir1040.   DOI
9 MHLW (Ministry of Health, Labour and Welfare). 2011. Retrieved from http://www.mhlw.go.jp/file/06-Seisakujouhou-11130500-Shokuhinanzenbu/0000124372.pdf on Sep 17, 2017
10 Moran JDW, Whitaker DJ and Kent ML. 1999. A review of the myxosporean genus Kudoa Meglitsch, 1947, and its impact on the international aquaculture industry and commercial fisheries. Aquaculture 172, 163-196. https://doi.org/10.1016/s0044-8486(98)00437-2.   DOI
11 Song JY, Kim MJ, Choi HS and Jung SH. 2014. Monitoring Kudoa septempunctata in Cultured Olive Flounder Paralichthys olivaceus in Different Regions of Korea in 2013. Korean J Fish Aquat Sci 47, 611-621. https://doi.org/10.5657/kfas.2014.0611.   DOI
12 St-Hilaire S, Ribble C, Whitaker DJ and Kent ML. 1997. Evaluation of a nondestructive diagnostic test for Kudoa thyrsites in farmed Atlantic salmon (Salmo salar). Aquaculture 156, 139-144. https://doi.org/10.1016/s0044-8486(97)00081-1.   DOI
13 Yokoyama H, Whipps CM, Kent ML, Mizuno K and Kawakami H. 2004. Kudoa thyrsites from Japanese flounder and Kudoa lateolabracis n. sp. from Chinese sea bass: causative myxozoans of post-mortem myoliquefaction. Fish Pathol 39, 79-86. https://doi.org/10.3147/jsfp.39.79.   DOI
14 MOF (Ministry of Oceans and Fisheries) 2015. Hygienic management guidelines for domestic flounder farm. MOF, Sejong, Korea.