• Korean Journal of Agricultural Science
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• v.43 no.1
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• pp.100-108
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• 2016

An auxin-producing bacteria (strain 5-1) was isolated from button mushroom compost in Boryeong-Si, Chungcheongnam-Do. The 5-1 strain was classified as a novel strain of Enterobacter aerogenes based on chemotaxonomic and phylogenetic analyses. The isolated E. aerogenes 5-1 was confirmed to produce indole-3-acetic acid (IAA), one of the auxin hormones, using TLC and HPLC analyses. When the concentration of IAA was assessed by performing HPLC quantitative analysis, a maximum concentration of IAA of $109.9mgL^{-1}$ was detected in the culture broth incubated in R2A medium containing 0.1% L-tryptophan for 24 h at $35^{\circ}C$. Acidification of the culture was deemed caused by an increase of IAA because a negative relationship between IAA production and pH was observed. Supplementation with a known precursor of IAA production, L-tryptophan, appeared to induce maximal production at 0.1% concentration, but it reduced production at concentrations above 0.2%. To investigate the growth-promoting effects to crops, the culture broth of E. aerogenes 5-1 was used to inoculate water cultures and seed pots of mung bean and lettuce. In consequence, adventitious root induction and root growth of mung bean and lettuce were two times higher than those of the control.

• The Plant Pathology Journal
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• v.23 no.2
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• pp.37-44
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• 2007

Colletotrichum contains many important pathogens which cause economically significant diseases of crops like pepper, strawberry, tomato and apple. Forty four isolates were collected to characterize the diversity of Colletotrichum causing apple anthracnose in various regions of Korea. They were analyzed by random amplified polymorphic DNA (RAPD), internal transcribed spacer (ITS) of rDNA and partial $\beta$-tubulin gene DNA sequence, and culture characteristics on PDA and PDA-Benomyl. From the results of molecular analyses, 31 strains belonged to Colletotrichum gloeosporioides, ribosomal DNA group (RG) 4 of Moriwaki et al. (2002), 8 strains belonged to C. acutatum, A2 group of Talhinhas et al. (2005) and 5 strains to C. acutatum, A3 group of Talhinhas et al. (2005). Most isolates of C. gloeosporioides RG4 grew faster on PDA than strains of C. acutatum, A2 and A3 groups and most RG4 strains were sensitive to Benomyl. However, a few strains of RG4 grew slower and were resistant to Benomyl. On the basis of molecular characteristics, apple isolates of C. acutatum were clearly differentiated from red pepper isolates of the species, but apple isolates of C. gloeosporioides were not.

• Journal of Microbiology and Biotechnology
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• v.23 no.8
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• pp.1147-1153
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• 2013

Pectobacterium carotovorum subsp. carotovorum (formerly Erwinia carotovora subsp. carotovora) is a plant pathogen that causes soft rot and stem rot diseases in several crops, including Chinese cabbage, potato, and tomato. To control this bacterium, we isolated a bacteriophage, PP1, with lytic activity against P. carotovorum subsp. carotovorum. Transmission electron microscopy revealed that the PP1 phage belongs to the Podoviridae family of the order Caudovirales, which exhibit icosahedral heads and short non-contractile tails. PP1 phage showed high specificity for P. carotovorum subsp. carotovorum, and several bacteria belonging to different species and phyla were resistant to PP1. This phage showed rapid and strong lytic activity against its host bacteria in liquid medium and was stable over a broad range of pH values. Disease caused by P. carotovorum subsp. carotovorum was significantly reduced by PP1 treatment. Overall, PP1 bacteriophage effectively controls P. carotovorum subsp. carotovorum.

• Korean journal of applied entomology
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• v.42 no.4
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• pp.301-305
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• 2003

This study investigated the ecological characteristics of Frankliniella occidentalis on four horticultural crops. On red pepper leaves, larval developmental period and survival rate of F. occidentalis were 6.4 days and 67%, respectively. While adult female lived longer on cucumber leaves as long as 16 days, on Petal lived 8.1 days. F. occidentalis adult female preferred tomato to eggplant, cucumber and red pepper, and higher number of hatched larva were from leaves of middle part of egg-plant compared with those from other parts. F. occidentalis adult female fed preferably on petal compared with leaves and Petals of eggplant, and among petals of four tested plants, the most preference was those of red pepper.

• Journal of Plant Biotechnology
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• v.45 no.3
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• pp.228-235
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• 2018

In this study, two pepper varieties, PRH1 (powdery mildew resistance line) and Saengryeg (powdery mildew resistance line), were resequenced using next generation sequencing technology in order to develop InDel markers. The genome-wide discovery of InDel variation was performed by comparing the whole-genome resequencing data of two pepper varieties to the Capsicum annuum cv. CM334 reference genome. A total of 334,236 and 318,256 InDels were identified in PRH1 and Saengryeg, respectively. The greatest number of homozygous InDels were discovered on chromosome 1 in PRH1 (24,954) and on chromosome 10 (29,552) in Saengryeg. Among these homozygous InDels, 19,094 and 4,885 InDels were distributed in the genic regions of PRH1 and Saengryeg, respectively, and 198,570 and 183,468 InDels were distributed in the intergenic regions. We have identified 197,821 polymorphic InDels between PRH1 and Saengryeg. A total of 11,697 primers sets were generated, resulting in the discovery of four polymorphic InDel markers. These new markers will be utilized in order to identify disease resistance genotypes in breeding populations. Therefore, our results will make a one-step advancement in whole genome resequencing and add genetic resource datasets in pepper breeding research.

• Journal of Forest and Environmental Science
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• v.31 no.4
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• pp.272-279
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• 2015

Xanthoceras sorbifolium is considered as bio-energy crops owing to the high oil content from kernel. This study was performed to analyze calorific value, crude ash content, ultimate ratio, crude lipid and fatty acid composition among seed sources. Calorific values ranged from $4,526.0\;cal\;g^{-1}$ to $7,377.2\;cal\;g^{-1}$ in seeds and kernels showed the highest value. Calorific values and crude ash contents were observed as significant difference among plantations and/or individuals (p>0.05). Kernel from SD-F plantation showed the highest calorific value and lower crude ash content. C content comprised 63.4%, the highest levels was detected from SD-F (64.8%). Crude lipid content in kernel observed as 54.5 g $100\;g^{-1}$ from SD-F. In contrast it was determined the lowest value from LN-JARS as 46.5 g $100\;g^{-1}$. The fatty acid composition of kernel was determined to those of oleic acid (31.3%) and linoleic acid (38.1%) from SD-F and LN-JARS. These results will be offered to useful information for breeding materials selection.

• Korean Journal of Environmental Agriculture
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• v.29 no.4
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• pp.408-416
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• 2010

To evaluate the effectiveness of AMF on the growth of horticultural crops, we compared mycorrhizal and non-mycorrhizal plants, perilla (P. frutescens Britt.), that were inoculated with AMF propagules. In the early stages of growth of perilla, compared to the AMF- perilla seedlings, in AMF+ perilla seedlings at 3 weeks after sowing, leaf length and width increased 17% and 29%, leaf area increased 28%, and shoot fresh weight increased 33%, root total length increased 1%, and chlorophyll content increased 3%. Further at 10 weeks after sowing, compared to the AMF- perilla plants, in perilla plants inoculated with AMF at the sowing and transplanting stages, leaf area increased 21% and 19%, shoot length increased 19% and 17%, root fresh weight increased 17% and 20%, and chlorophyll content increased 5.1% and 4.8%, respectively. Moreover, at 14 weeks after sowing, compared to the AMFperilla plants, in perilla plants inoculated with AMF at the sowing and transplanting stages, the number of leaves increased 16% and 20%, root fresh weight increased 16% and 17% significantly. Further, leaf fresh weight increased 9% and 11%, shoot diameter increased 4.5% and 7.3%, and chlorophyll content increased 1.5% and 2.5%, respectively. The levels of many macronutrients and micronutrients were tended to be significantly higher in AMF+ plants than in AMF- plants, supporting the association between AMF and enhanced growth of plants grown from AMF+ seedlings.

• Journal of Plant Biotechnology
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• v.37 no.4
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• pp.379-387
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• 2010

Oilseed rape (Brassica napus L.) is an important crop due to its high oil content in the seed. Recently, the demand for the improvement of crop for biodisel energy source is increased as oil prices in the world has increased dramatically. Until now, oilseed rape breeding was carried out by cross-hybridization between different varieties and related germplasms. However, like as many other crops, the application of tissue culture and gene transformation systems has been introduced into oilseed rape breeding program including the development of transgenic canola plants. In this study, we reviewed a history of tissue culture and genetic transformation research in oilseed rape plants and indicated some important aspects for the production of transgenic oilseed rape plants.

• Journal of Plant Biotechnology
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• v.37 no.4
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• pp.456-464
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• 2010

Potato is one of the most important crops in the world. Due to vegetative propagation of this crop, techniques of plant tissue culture and genetic transformation are often applied for potato researches and a lot of progress has been made in the breeding programs using these techniques during the last decades. In potato, there have been several trials to introduce GM potato varieties to the world market, but they so far failed due to the changed legislation and unwillingness of large processors to process GM potatoes. These issues are highly associated with the general acceptances of the public and other political decisions. In addition to these, there are still obstacles to overcome to achieve the development of commercial potato variety and several factors to improve horticulturally important traits. In this study, therefore, we reviewed recent advances and research status on tissue culture and genetic transformation in potato and discussed future perspective.

• Journal of People, Plants, and Environment
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• v.24 no.6
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• pp.673-684
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• 2021

Background and objective: This study seeks to investigate domestic agro-healing farm resources so that preliminary data can be obtained on the systematic provisions for agro-healing programs in order to improve service quality and to identify the quality of agro-healing policy services. Methods: For this study, owners of agro-healing facilities were asked 24 questions via an online survey. An importance-performance analysis (IPA) was conducted to identify service quality for each policy sector. Results: Respondents' satisfaction levels were low when compared to agro-healing farm owners' perceptions of the above-average importance of each sector. The results from the resource analysis of domestic agro-healing farms indicated that the main operational purposes of farms were, in descending order, experience (90.0%), healing (86.7%), and education (84.0%). With respect to the type of program that was operated, cultivating crops (horticultural therapy) was identified most frequently at 83.3%. Conclusion: As a result of this study, the resources possessed by agro-healing farms were identified. In addition, the results of the IPA analysis in terms of service quality are not as good as expected, and performance levels should be increased to improve this. The results of this study are expected to provide useful information not only to improve the quality of agro-healing services, but also to revitalize the agro-healing industry while developing a systematic agro-healing program.