• Plant Resources
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• 제5권1호
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• pp.74-77
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• 2002

This study was performed to investigate the effect of Leonuri Herba extract on the treatment of chromaticity and colorfastness. Leonuri Herba has been used as a Korean medicine for the treatment of woman disease. In the long history of Korea, dyeing has been applied for a means representing the grace of natural and inner esthetic consciousness of man. Vegetable dyes give us such great benefits, diversified color, but no pollution. And ramie fabric has distinctive features such as beautiful brilliance, elegance, and strong durability. So, it is regarded as a special product of Korea traditionally. These studies were carried out to treat with acetate iron, dichloride copper and alum with a mordant to ramie fabric. The ramie fabric was died with Leonuri Herba extract. The results of experiment showed as follows: First, the chromophoric degree was the highest in acetate iron but not distinction in another mordants. Seocond, the light colorfastness was the highest in non treated and dichloride copper, but alum was the lowest. Third, the discoloration was alum and dichloride copper showed first grade in washing colorfastness. Abrasion colorfastness was not significant in this test. According the previous results, Leonuri Herba has an efficiency on woman disease. So it is considered that Leonuri Herba can be applied effectively to a theraphy of climacteric disturbance.

• 대한본초학회지
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• 제25권2호
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• pp.107-116
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• 2010

Objectives : This study purposed to research the anti-cancer effects of Andrographitis Herba. Methods : By measuring the cell proliferation, apoptosis, morphology and cytokine level from the extracts, the influence on a A549 cell was compared. Results : The Andrographitis Herba decoction extract according to the concentration inhibited the proliferation and increased the apoptosis of the A549 cell. Among the various fraction extracts of the Andrographitis Herba decoction, EtOEt showed the greatest increase of the apoptosis of the A549 cell. The Andrographitis Herba decoction extract according to the concentration decreased the secretion of the TGF-$\beta$ in the A549 cell, and increased the secretion of the TNF-$\alpha$ and the IFN-$\gamma$ presenting cell population. Conclusion : It is considered that the total extract and various fraction extracts of Andrographitis Herba decoction inhibit the proliferation of A549 cells.

• 동아시아식생활학회지
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• 제5권3호
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• pp.215-221
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• 1995

This study was undertaken to investigate the effect of Taraxacum herba extract on the hepatic xanthine oxidase activity as a oxygen free radical generating enzyme in vitro and in vivo. It was observed that partial purified hepatic xanthine oxidase (type O) activity was strongly inhibited by the addition of Taraxacum herba n-butanol extract in vitro. The Km value of xanthine oxidase without affecting the Vmax value for xanthine was significantly increased by the addition of ta-dase (type O) activity was significantly inhibited by the treatment of Taraxacum gerba n-butanol ex-tract for 5days(over 40mg/kg, i.p), whereas, xanthine oxidase (type D) activity was not changed by the injection of Taracacum herba n-butanol extract. Meanwhile, liver weight / body weight(%), serum alanine aminotransferase activity and hepatic lipid peroxide content in Taraxacum herba n-buta-nol extract-treated rat were not changed. These findings led us to conclude that Taraxacum herba n-butanol extract may regulate the hepatic xanthine oxidase type O activity to prevent toxic effect of oxidative stress by the oxygen free radicals.

• 동의생리병리학회지
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• 제21권3호
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• pp.705-708
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• 2007

The objective of the study was to observe the anti-diabetic effect of Solani nigri Herba(SH). The following experimental results were derived from the measurement of the levels of glucose, GOT and GPT in the serum and weight from a group of experimental rats by way of the injection of streptozotocin as well as the oral administration of Solani nigri Herba water extracts. The glucose level in the serum significantly decreased in the Solani nigri Herba-dosed group(SH2,12.6 mg/100 g rat weight) on the 10th day. The GOT level of SHI(4.2 mg/100 g Solani nigri Herba) and SH2 in the serum slightly decreased, but no significance to compare with control group. The GPT level of SH1 and SH2 in the serum slightly decreased, but no significance to compare with control group. The weight of rat of SH1 and SH2 in the serum slightly increased, but no significance to compare with control group. In conclusion, Solani nigri Herba appeared to have significance inhibitory effects on the rise of blood sugar level in the experimentally induced diabetics.

• 한국식물병리학회지
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• 제14권2호
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• pp.184-185
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• 1998

Rhizoctonia stem rot of Saururui herba [Saururus chinensis (Lour) Baill] was observed during plantations from 1996 to 1997 in Chungbuk area. Infected plant showed damping-off and stem rot at soil line. The causal fungus of stem rot isolated from the infected plant was identified as Rhizoctonia solani according to the criteria based on the cultural and morphological characteristics. The stem rot of Saururi herba caused by Rhizoctonia solani was first described in Korea, and the name the“Rhizoctonia black stem rot”is proposed.

• 대한본초학회지
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• 제23권2호
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• pp.1-8
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• 2008

Objectives : The purpose of this study was to evaluate on the antimicrobial effect on the periodontal pathogens and anti-inflammatory effect of Artemisiae Iwayomogii Herba. Artemisiae Iwayomogii Herba has been used for treating as Artemisiae Capilaris Herba in Korea. Methods : Artemisiae Iwayomogii Herba was prepared by extracting medicinal herb with water. We investigated antimicrobial activity by the minimun inhibitory concentration (MIC) test. We also investigated inhibition of IL-$1{\beta}$-induced collagenase-l(MMP-l), stromelysin-1(MMP-3), interleukin-6 gene expression in human gingival fibroblasts. Results : The antimicrobial effect of Artemisiae Iwayomogii Herba was evaluated with MIC against periodontopathogens; Porphyromonas gingivalis 2561, W50, A7A1-28, 9-14K-1, Prevotella intermedia28, and Actinobacillus actinomycetemcomitans Y4, MICs of Artemisiae Iwayomogii Herba were 0.156 mg/ml, 0.625 mg/ml, 0.313 mg/ml, 1.25 mg/ml, 10 mg/ml and 10 mg/ml. The anti-inflammatory effect of Artemisiae Iwayomogii Herba was evaluated with Influence of herbs on the IL-$1{\beta}$-induced expression of MMP-1, MMP-3, interleukin-6, IL-$1{\beta}$ increased MMP-1, MMP-3, interleukin-6 mRNA levels. Artemisiae Iwayomogii Herba significantly inhibited IL-$1{\beta}$-induced MMP-1, MMP-3, interleukin-6 gene expressions in a dose-dependent manner. Conclusions : These results suggested that Artemisiae Iwayomogii Herba might reduce the excessive proteolytic capacity of the gingival fibroblast during inflammation and could be developed a new drug in periodontitis.

• 동의생리병리학회지
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• 제20권4호
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• pp.936-941
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• 2006

To prevent human body injury from oxidative stress, antioxidants are very important and many research about antioxidants are generally being conducted. Hydrogen peroxide$(H_20_2)$ that is one of vitality oxygen species has been seen that cause various diseases, DNA damage and gene change. We have already known that the inhibition effect of Zizania latifolia Radix, Rhizoma on apoptosis induced by $H_2O_2$ in Neuro2A cell. And the purpose of this study was that we made a comparative study on the inhibition effect of apoptosis in Neuro2A cell on the region of Zizania latifolia(Radix, Rhizoma, Herba). Neuro2A cells were cultivated in RPMI(GibcoBRL) with 5% FBS and treated with $H_2O_2$ and Zizania latifolia(Radix, Rhizoma, Herba). Separately we measured the cell viability and analyzed DNA fragmentation. Activity of PARP, Cytochrome C, caspase-9, caspase-3, p53, p21, Bax and Bcl-2 in the cell was examined by using western blot. The results obtained were as Follows: The cell viability in all of Zizania latifolia (Radix, Rhizoma, Herba) treatment (60ug/m1<) decreased significantly compared with that of none treatment(p<0.001). Zizania latifolia Radix increased cell viability was most effective of three regions. But we had no significant difference among three regions. All of Zizania latifolia (Radix, Rhizoma, Herba) increased cell viability about twice as much as that being injury by $H_2O_2$,(Zizania Latifolia (Radix, nhizoma, Herba) 20ug/m1, $H_2O_2$ 200uM, p<0.001). DNA fragmentation developed by $H_2O_2$, but was not developed in all of Zizania latifolia (Radix, Rhizoma, Herba) treatment. PARP, Cytochrome C, caspase-9 and caspase-3 activated all by $H_2O_2$ but were not activated in all of Zizania latifolia (Radix, Rhizoma, Herba) treatment. P53, P2l and Bax activated by $H_2O_2$, and Bcl-2 got into inactivation. But the opposite results appeared in all of Zizania latifolia (Radix, Rhizoma, Herba) treatment. In conclusion, these results suggest that all of Zizania latifolia (Radix, Rhizoma, Herba) inhibit the development of DNA fragmentation and apoptosis by $H_2O_2$and the antioridant action of all of Zizania latifolia (Radix, Rhizoma, Herba) is effective.

• 대한본초학회지
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• 제25권3호
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• pp.81-90
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• 2010

Objectives : The present study purposed to compare the effects of Melandrii Herba and Vaccariae Semen, which are used as Wang-Bul-Yu-Haeng(王不留行) with vasodilatory effect in Korea and China, respectively, on hyperlipidemia. Methods : We applied the water extract of Melandrii Herba and Vaccariae Semen to rats with hyperlipidemia induced by high-cholesterol diet and high-fructose diet and evaluated their effects. Results : While Vaccariae Semen had significant effect on the reduction of the levels of total cholesterol, LDL-cholesterol, free cholesterol, and the activation of ALT, the application of Melandrii Herba had no significant effect on the levels of total cholesterol, LDL-cholesterol, free cholesterol, neutral fat, and free fatty acid, and the activation of AST. For the rats with hypertriglyceridemia, Vaccariae Semen reduced the levels of neutral fat and total cholesterol, but did not significantly reduce the level of free fatty acid and the activation of AST and ALT. The application of Melandrii Herba did not have any significant effect on the levels of neutral fat, total cholesterol, and free fatty acid, and the activation of AST and ALT, but it increased the level of HDL cholesterol. Conclusion : Vaccariae Semen showed a possibility as a remedy for hyperlipidemia, but Melandrii Herba did not show any significant effect. Thus, based on such a difference in remedial effect between Melandrii Herba and Vaccariae Semen, they need to be distinguished in clinical application.

• 대한한방내과학회지
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• 제39권3호
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• pp.302-312
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• 2018

Objectives: This study was performed to investigate the effects of two herbal medicines, Agastachis Herba and Lysimachiae Herba, on a cellular model of non-alcoholic fatty liver diseases (NFLDs). Methods: HepG2 cells were treated with palmitic acid and with various concentrations of Agastachis Herba (AH) or Lysimachiae Herba (LH) extract in water. The lipotoxicity was assessed using EZ-cytox, and the lipoapoptosis was assessed using cell death detection ELISA. Intracellular lipids were measured by oil red O staining. The efficacy of AH and LH on sterol regulatory element-binding transcription factor-1c (SREBP-1c), fatty acid synthase (FAS), and acetyl-CoA carboxylase (ACC) in HepG2 cells was measured by reverse transcription polymerase chain reaction (RT-PCR). Results: Both AH and LH extracts increased lipoapoptosis and decreased lipotoxicity and levels of SREBP-1c, ACC, and FAS (SREBP-1c, ACC, and FAS are factors in lipid synthesis). In the oil red O staining experiment, both extracts also reduced intracellular lipid accumulation; in this instance, LH's efficacy was superior to that of AH. Conclusions: According to the results, both AH and LH are likely to contribute to non-alcoholic fatty liver disease, as both interfere with lipid synthesis.

• 한방안이비인후피부과학회지
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• 제22권2호
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• pp.19-38
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• 2009

Objective : The purpose of this study is to find out whether Herba Ephedrae, solely used, and Herbal medicine in which this was included, have inhibitory effects of Nitric Oxide(NO). Methods : We tested the inhibitory effects of Nitric Oxide(NO) with Herba Ephedrae and ten kinds of Herbal medicine combined with Herba Ephedrae(Worlbikachul-Tang, 越婢加朮湯; Mahaengkamsuk-Tang, 麻杏甘石湯; Shinbi-Tang, 神秘湯; Mahwangbujaseshin-Tang, 麻黃附子細辛湯; Euiin-Tang, 薏苡仁湯; Galgeun-Tang, 葛根湯; Mahaengeuigam-Tang, 麻杏薏甘湯; Mahwang-Tang, 麻黃湯; Socheongryong-Tang, 小靑龍湯; Gaemagakban-Tang, 桂麻各半湯) on RAW264.7 cells. Results and Conclusions : 1. We carried out MTT assay on Herba Ephedrae and those decoctions including this in order to determine whether they accommodate cotytoxicity. The results were that Worlbikachul-Tang, Mahaengkamsuk-Tang, Mahwangbujaseshin-Tang, Mahaengeuigam-Tang, Mahwang-Tang, Socheongryong-Tang and Gaemagakban-Tang showed no cytotoxicity on RAW264.7 with 0.1mg/ml and 0.5mg/ml dosages of decoctions but displayed cytotoxicity on the cell with 1mg/ml. Solely used Herba Ephedrae, Shinbi-Tang, Euiin-Tang and Galgeun-Tang exhibited cytotoxicity beyond the concentration of 0.5mg/ml. 2. Worlbikachul-Tang, Mahaengkamsuk-Tang, Shinbi-Tang, Mahwangbujaseshin-Tang, Euiin-Tang, Galgeun-Tang, Mahaengeuigam-Tang, Mahwang-Tang and Socheongryong-Tang showed inhibition of NO production but solely used Herba Ephedrae and Gaemagakban-Tang did not exhibit such reaction.