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http://dx.doi.org/10.22246/jikm.2018.39.3.302

Effects of Agastachis Herba Extract and Lysimachiae Herba Extract on the Experimental Cellular Model of NFLDs Induced by Palmitic Acid  

Lee, Hye-in (Dept. of Internal Medicine, College of Korean Medicine, Dong-Shin University)
Kim, Young-kwang (Dept. of Internal Medicine, College of Korean Medicine, Dong-Shin University)
Lim, Hyeon-chan (Dept. of Internal Medicine, College of Korean Medicine, Dong-Shin University)
Lee, Da-eun (Dept. of Internal Medicine, College of Korean Medicine, Dong-Shin University)
Kim, Eun-ji (Dept. of Internal Medicine, College of Korean Medicine, Dong-Shin University)
Moon, Young-ho (Dept. of Internal Medicine, College of Korean Medicine, Dong-Shin University)
Publication Information
The Journal of Internal Korean Medicine / v.39, no.3, 2018 , pp. 302-312 More about this Journal
Abstract
Objectives: This study was performed to investigate the effects of two herbal medicines, Agastachis Herba and Lysimachiae Herba, on a cellular model of non-alcoholic fatty liver diseases (NFLDs). Methods: HepG2 cells were treated with palmitic acid and with various concentrations of Agastachis Herba (AH) or Lysimachiae Herba (LH) extract in water. The lipotoxicity was assessed using EZ-cytox, and the lipoapoptosis was assessed using cell death detection ELISA. Intracellular lipids were measured by oil red O staining. The efficacy of AH and LH on sterol regulatory element-binding transcription factor-1c (SREBP-1c), fatty acid synthase (FAS), and acetyl-CoA carboxylase (ACC) in HepG2 cells was measured by reverse transcription polymerase chain reaction (RT-PCR). Results: Both AH and LH extracts increased lipoapoptosis and decreased lipotoxicity and levels of SREBP-1c, ACC, and FAS (SREBP-1c, ACC, and FAS are factors in lipid synthesis). In the oil red O staining experiment, both extracts also reduced intracellular lipid accumulation; in this instance, LH's efficacy was superior to that of AH. Conclusions: According to the results, both AH and LH are likely to contribute to non-alcoholic fatty liver disease, as both interfere with lipid synthesis.
Keywords
Agastachis Herba; Lysimachiae Herba; NAFLD;
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