대한한방내과학회지 (The Journal of Internal Korean Medicine)

대한한방내과학회 (The Society of Internal Korean Medicine)
권호 표지
DOI QR코드

DOI QR Code

곽향과 금전초 추출물이 Palmitic acid로 유발된 비알코올성 지방간 세포 모델에 미치는 영향

Effects of Agastachis Herba Extract and Lysimachiae Herba Extract on the Experimental Cellular Model of NFLDs Induced by Palmitic Acid

  • 이혜인 (동신대학교 한의과대학 내과학교실) ;
  • 김영광 (동신대학교 한의과대학 내과학교실) ;
  • 임현찬 (동신대학교 한의과대학 내과학교실) ;
  • 이다은 (동신대학교 한의과대학 내과학교실) ;
  • 김은지 (동신대학교 한의과대학 내과학교실) ;
  • 문영호 (동신대학교 한의과대학 내과학교실)
  • Lee, Hye-in (Dept. of Internal Medicine, College of Korean Medicine, Dong-Shin University) ;
  • Kim, Young-kwang (Dept. of Internal Medicine, College of Korean Medicine, Dong-Shin University) ;
  • Lim, Hyeon-chan (Dept. of Internal Medicine, College of Korean Medicine, Dong-Shin University) ;
  • Lee, Da-eun (Dept. of Internal Medicine, College of Korean Medicine, Dong-Shin University) ;
  • Kim, Eun-ji (Dept. of Internal Medicine, College of Korean Medicine, Dong-Shin University) ;
  • Moon, Young-ho (Dept. of Internal Medicine, College of Korean Medicine, Dong-Shin University)
  • 투고 : 2018.03.28
  • 심사 : 2018.06.28
  • 발행 : 2018.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objectives: This study was performed to investigate the effects of two herbal medicines, Agastachis Herba and Lysimachiae Herba, on a cellular model of non-alcoholic fatty liver diseases (NFLDs). Methods: HepG2 cells were treated with palmitic acid and with various concentrations of Agastachis Herba (AH) or Lysimachiae Herba (LH) extract in water. The lipotoxicity was assessed using EZ-cytox, and the lipoapoptosis was assessed using cell death detection ELISA. Intracellular lipids were measured by oil red O staining. The efficacy of AH and LH on sterol regulatory element-binding transcription factor-1c (SREBP-1c), fatty acid synthase (FAS), and acetyl-CoA carboxylase (ACC) in HepG2 cells was measured by reverse transcription polymerase chain reaction (RT-PCR). Results: Both AH and LH extracts increased lipoapoptosis and decreased lipotoxicity and levels of SREBP-1c, ACC, and FAS (SREBP-1c, ACC, and FAS are factors in lipid synthesis). In the oil red O staining experiment, both extracts also reduced intracellular lipid accumulation; in this instance, LH's efficacy was superior to that of AH. Conclusions: According to the results, both AH and LH are likely to contribute to non-alcoholic fatty liver disease, as both interfere with lipid synthesis.

키워드

참고문헌

  1. Department of Internal Medicine, Medical school of Seoul University. 2005 recent Advances in Internal Medicine. Seoul: Korean Medical book Publishing; 2005, p. 138-49.
  2. Foster C, Mistry NF, Peddi PF, Sharma S. The Washington Manual of Medical Therapeutics. 33rd edition. Seoul: Shinheung Medscience; 2012, p. 711-3.
  3. Rhee EJ. Clinical Characteristics of Non-Alcoholic Fatty Liver Disease Based on Analyses from the Kangbuk Samsung Health Study. J Korean Diabetes 2017;18(2):82-7.
  4. The Korean Association for the Study of the Liver. Non-Alcoholic Fatty Liver Disease Guidelines. 1st edition. Seoul: Jin Publishing; 2013, p. 1.
  5. Jo JH, Ju JH, Jang JW. Road Map Clinical Internal Medicine. 1st edition. Seoul: Panmun Education; 2012, p. 80.
  6. Kim W. Treatment Options in Non-alcoholic Fatty Liver Disease. Korean J Gastroenterol 2017;69(6):353-8. https://doi.org/10.4166/kjg.2017.69.6.353
  7. Department of Liver Internal Medicine, College of Korean Medicine. Liver Internal Medicine. 5th edition. Seoul: Nado Publishing; 2013, p. 102-15, 321-28.
  8. Kim SY, Kwon JN, Lee I, Hong JW, Choi JY, Park SH, et al. Research on Anti-lipogenic Effect and Underlying Mechanism of Laminaria japonica on Experimental Cellular Model of Non-alcoholic Fatty Liver Disease. J Int Korean Med 2014;35(2):175-83.
  9. Jang YS, Seo JY, Kwun MJ, Kwon JN, Lee I, Hong JW, et al. Effect of Phaseoulusangularis Seed on Experimental Cellular Model of Nonalcoholic Fatty Liver Desease. Korean J Oreintal Physiology & Pathology 2013;27(6):802-8.
  10. Han CW, Lee JH. Effects of KHchunggan-tang on the Nonalcoholic Fatty Liver Disease in Palmitate-induced Cellular Model. J Korean Oriental Med 2011;32(1):109-20.
  11. Kim IR, Kim HC, Park SJ, Park YG, Park JH, Seo BI, et al. Herbal medicine. 2nd edition. Seoul: Younglim Publishing; 2010, p. 335-6, 370-1.
  12. Kim YM, Kim MH, Yang WM. Effects of Agastacherugosa on Obesity Via Inhibition of Peroxisome Proliferator-Activated Receptor-Gamma and Reduction of Food Intake. J Korean Med Obes Res 2015;15(2):104-10. https://doi.org/10.15429/jkomor.2015.15.2.104
  13. Jang MH, Byun SH, Seo HL. Hepatoprotective effect of Teucriumveronicoides. J East-West Medicines 2015;40(1):1-12.
  14. Bae GS, Park HJ, Kim DY, Seo SW, Park KB, Kim BJ, et al. Inhibitory Effect of Extract of Teucriumveronicoides on the Production of Inflammatory Cytokines. J Korea Herbology 2008;23(3):119-25.
  15. Kim MS, Kim KO, Kim KS, Park SY, Yang SJ, Wei TS, et al. Effects of Lysimachiae Herbal extracts on Hyperlipidemic mice. J Korean Herbal Formula Study 2015;23(1)91-9. https://doi.org/10.14374/HFS.2015.23.1.091
  16. Kim OK. Antidiabetic Effect of Glechoma hederacea LINNAEUS in Streptozotocin-Induced Diabetic Rats. Korea J Pharmacogn 2004;35(4):300-8.
  17. Lee HG, Byun JS, Buyn SH, Kim SC. The Effects of Glechomalongituba on Rats Fed High Diet. J Korean Oriental Medical Prescription 2002;10(1):61-72.
  18. Choi WC. Nonalcoholic fatty liver. Inje Medical Journal 2002:23(2):217-24.
  19. Park SH, Pharmacologic Therapy for Nonalcoholic Fatty Liver Disease. Korean J Med 2014;86(4):425-9. https://doi.org/10.3904/kjm.2014.86.4.425
  20. Salah KB, Mahjoub MA, Chaumont JP, Michel L, Millet-Clerc J, Chraeif I, et al. Chemical compostion and in vitro antifungal and antioxidant activity of the essential oil and methanolic extract of teucriumsauvagei Le Houerou. Natl Prod Res 2006;20(12):1089-97. https://doi.org/10.1080/14786410600704748
  21. Esmaeili MA, Yazdanparast R. Hypoglycaemic effect of Teucriumpolium: studies with rat pancreatic islets. J Ethnopharmacol 2004;95(1):27-30. https://doi.org/10.1016/j.jep.2004.06.023
  22. Suleiman MS, Abdul-Ghani AS, Al-khalil S, Amin R. Effect of Teucriumpolium boiled leaf extract on intestinal motility and blood pressure. J Ethnopharmacol 1988;22(1):111-6. https://doi.org/10.1016/0378-8741(88)90236-X
  23. Shin DG, Kim DK, Lee JY. A Clinical Study on the Effects of Gwakhyangjunggi-san gamibag on 9 Obese Children. J Pediatrics of Korean Medicine 2001;15(1):183-94.
  24. Choi JW, Park JC, Lee CK. Biologic Activities of LysimachiaeHerba II-Analgesic and antiinflammatory effects of ethyl acetate fraction and a phenyl propanoid component. Natural Product Sciences 1997;3(2):135-40.
  25. Repa JJ, Liang G, Ou J, Bashmakov Y, Lobaccaro JM, Shimomura I, et al. Regulation of mouse sterol regulatory element-binding protein-1c gene(SREBP-1c) by oxysterol receptors, LXRalpha and LXRbeta. Genes Dev 2000;14(22):2819-30. https://doi.org/10.1101/gad.844900

피인용 문헌

  1. 비알코올성 지방간질환의 이수(利水) 약재 포함 한약 치료에 대한 최신 임상 연구 동향 - CAJ 검색을 중심으로 vol.40, pp.1, 2018, https://doi.org/10.22246/jikm.2019.40.1.38
  2. 간 실질세포주에서 니호채(泥胡菜) 분획물이 liver X receptor α 의존적 지방 생성 유전자의 발현에 미치는 효과 vol.28, pp.3, 2020, https://doi.org/10.14374/hfs.2020.28.3.255