• Title/Summary/Keyword: Heo Jong

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Cellular protective effect of Ecklonia cava extract on ultra-fine dust (PM2.5)-induced cytoxicity (초미세먼지(PM2.5)로 유도된 in vitro 세포 독성에 대한 감태(Ecklonia cava) 추출물의 보호 효과)

  • Park, Seon Kyeong;Kang, Jin Yong;Kim, Jong Min;Yoo, Seul Ki;Han, Hye Ju;Shin, Eun Jin;Heo, Ho Jin
    • Korean Journal of Food Science and Technology
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    • v.51 no.5
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    • pp.503-508
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    • 2019
  • To evaluate the protective effect of Ecklonia cava on ultra-fine dust ($PM_{2.5}$)-induced cytotoxicity, we investigated the in vitro antioxidant activity and cell viability after exposure to $PM_{2.5}$. E. cava was extracted using water and 80% ethanol, and antioxidant activity was determined using the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS)/2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and lipid peroxidation inhibition assays. The 80% ethanol extract showed relatively higher antioxidant activity than the water extract. The cell protective effects were determined by measuring the intracellular reactive oxygen species (ROS) content and viability of nasal epithelial (RPMI-2650), lung epithelial (A549), and brain neuroblastoma (MC-IXC) cells. Results showed that the 80% ethanol extract inhibited ROS production more than the water extract. In contrast, both extracts showed similar effects on cell viability in the $PM_{2.5}$-induced cell death assay. Thus, Ecklonia cava may act as an effective resource for preventing $PM_{2.5}$-induced cytotoxicity in nasal, lung, and brain cells.

Analysis of Anti-Tumor Activity of Flammulina velutipes Extract on B16 cells (B16 흑색종에 대한 팽이버섯 추출물의 항암활성 비교)

  • Lee, Si-Rim;Nam, Dong-Yun;Lee, Hyun-Jin;Park, Chul-Hong;Heo, Jin-Chul;Kim, Jong-Guk;Lee, Jin-Man;Lee, Chang-Yun;Park, Hee-Ju;Lee, Sang-Han
    • Food Science and Preservation
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    • v.16 no.4
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    • pp.599-603
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    • 2009
  • To evaluate the anti-tumor activity of Flammulina velutipes extract, we used an in vitro wound-healing assay, and an in vivo approach using a mouse melanoma model. Wound-healing activity in B16 cells was affected by the extract in a dose-dependent manner, indicating that the extract had anti-metastatic activity. The extract also exhibited strong anti-tumor activity against lung cancer when B16 cells were injected into mouse veins together with B16 melanoma cells. The results indicatethat the Flammulina velutipes extract decreased B16 cancer cell growth by inhibition of cell migration both in vitro and in vivo.

State Indicator of Water Quality for Surface Water and Groundwater in Agriculture

  • Kim, Min-Kyeong;Jung, Goo-Bok;Ro, An-Sung;Choi, Seung-Chul;Choi, Won-Il;Kim, Eun-Jeong;Lee, Jong-Eun;Yun, Yeo-Uk;Kim, Kab-Cheol;Ko, Do-Young;Kim, Byeong-Ho;Kim, Hyeon-Ji;Park, Sang-Jo;Lee, Seong-Tae;Heo, Jae-Young;Yang, Sang-Ho;Kang, Seong-Soo
    • Korean Journal of Soil Science and Fertilizer
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    • v.48 no.5
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    • pp.509-514
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    • 2015
  • Indicators of environmental conditions qualitatively and quantitatively describe the state of the environment and natural resources, and the OECD (Organization for Economic Cooperation and Development) has initiated and suggested AEIs (Agri-Environmental Indicators) to assess trends over time of the effects of agriculture on the environment and the effectiveness and efficiency of agricultural and environmental policy measures since 1990's. This study aims to develop the state indicators of water quality for agricultural water, surface and groundwater, to evaluate the environmental impacts of agricultural activities and policies by qualifying the environmental levels of a nation. Status indicators were calculated according to the agricultural water quality standards of OECD and Korea, and their trends were analyzed over time. Particularly, nitrate ($NO_3{^-}$) status indicators of ground water in 2013 were significantly lower than the ones in 2000. Overall, the water quality indicators of surface water in 2013 were higher than the ones in 2012, except for pH and DO. The groundwater quality indicators in 2013 were lower than those of previous years. The optimal management indicators were calculated to assess agricultural surface water and groundwater quality. The findings of this study indicated that the state indicators could play a significant role to establish policies and procedures for managing and conserving water resources. This study also discussed water pollution caused by agricultural and industrial effluents.

Optimum Application Level of Liquid Pig Manure for Increasing Green Manure Crop Yield (녹비생산량 극대화를 위한 돈분액비의 최적 투입량 구명)

  • Lee, Snag-Gyu;Seo, Dong-Cheol;Kang, Se-Won;Seo, Young-Jin;Choi, Ik-Won;Kim, Min-Ok;Jeon, Won-Tai;Kang, Ui-Gum;Heo, Jong-Soo;Cho, Ju-Sik
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.6
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    • pp.1103-1106
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    • 2011
  • Optimum application level of liquid pig manure for increasing Astragalus sinicus L. (ASASI) and Lolium multiflorum Lam. (LOLMU) yield was investigated. The green manure crop yield were generally high in the order of ALPM 100 ${\geqq}$ APLM 75 ${\geqq}$ APLM 50 ${\geqq}$ APLM 0 in site 1 (ASASI), and LPLM 100 ${\geqq}$ LPLM 75 > LPLM 50 ${\geqq}$ LPLM 0 in site 2 (LOLMU). The optimum application level of liquid pig manure was 100% of nitrogen fertilization level.

Broiler responses to dietary 3,4,5-trihydroxybenzoic acid and oregano extracts under Eimeria challenge conditions

  • Yu, Myunghwan;Jeon, Jong Oh;Cho, Hyun Min;Hong, Jun Seon;Kim, Yu Bin;Nawarathne, Shan Randima;Wickramasuriya, Samiru Sudharaka;Yi, Young-Joo;Lee, Hans;Wan, Vannie;Ng, Noele Kai Jing;Tan, Chuan Hao;Heo, Jung Min
    • Journal of Animal Science and Technology
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    • v.63 no.6
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    • pp.1362-1375
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    • 2021
  • This study was conducted to evaluate the efficacy of a combination 3,4,5-trihydroxybenzoic acid (THB) and oregano extracts (i.e., Carvacrol and Thymol) at intake/dietary different levels on growth performance, intestinal health indicators, immune responses and fecal oocyst shedding in broiler chickens under Eimeria challenged condition. A total of 336 one-day-old broilers were randomly assigned to one of six dietary treatments with seven replications per treatment. Dietary treatments were: i) Non-challenged bird without any dietary treatment (NCNT), ii) Challenged bird without any dietary treatment (CNT), iii) Challenged birds fed a THB diet (0.1 g/kg, THB), iv) Challenged birds fed a combination of THB and oregano extracts diet (0.1 g/kg, COM 100), and a gradual increase of combination of THB and oregano extracts likely v) 0.15 g/kg (COM 150), and 0.2 g/kg (COM 200). On day 14, all groups except for NCNT have orally challenged with a 10-fold dose of Livacox® T anticoccidial vaccine to trigger coccidiosis. The results indicated that Eimeria-challenged broilers fed COM 100 and COM 200 diets increased (p < 0.05) body weight than CNT diet on day 35. Furthermore, birds fed COM 100 and COM 200 diets increased (p < 0.05) average daily gain compared to those fed CNT diets for the entire experimental period. There is no significant (p > 0.05) in average daily feed intake, feed efficiency between NCNT and birds fed with combined THB and oregano extracts for the entire experimental period. A combination of THB and oregano extract regardless of concentration levels or THB alone reduced (p < 0.05) lesion score in ileum compared to the CNT diet for 7 days post-infection (dpi). Birds fed COM 100 diet had lower (p < 0.05) intestinal lesion scores in jejunum and caeca on 7 dpi compared to those were in the CNT diet. No (p > 0.05) difference was observed in the oocysts per gram of feces count, intestinal morphology, carcass traits and blood cytokine concentration among the infected treatments. Collectively, we conclude that birds fed with a combination of THB and oregano extracts regardless of the ratios that were used demonstrated better recovery of health after the coccidial challenge than using only THB alone.

Improved Compressive·Flexural Performance of Hybrid Fiber-Reinforced Mortar Using Steel and Carbon Fibers (강 및 탄소 섬유를 사용한 하이브리드 섬유보강 모르타르의 압축·휨성능 향상)

  • Heo, Gwang-Hee;Park, Jong-Gun;Seo, Dong-Ju;Koh, Sung-Gon
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.25 no.5
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    • pp.48-59
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    • 2021
  • In this study, experiments were conducted to investigate the compressive·flexural performances of single fiber-reinforced mortar (FRM) using only steel fiber or carbon fiber which has different material properties as well as hybrid FRM using a mixture of steel and carbon fibers. The mortar specimens incorporated steel and carbon fibers in the mix proportions of 1+0%, 0.75+0.25%, 0.5+0.5%, 0.25+0.75% and 0+1% by volume at a total volume fraction of 1.0%. Their mechanical performance was compared and examined with a plain mortar without fiber at 28 days of age. The experiments of mortar showed that the hybrid FRM using a mixture of 0.75% steel fibers + 0.25% carbon fibers had the highest compressive and flexural strength, confirming by thus the synergistic reinforcing effect of the hybrid FRM. On the contrast, in the case of hybrid FRM using a mixture of 0.5% steel fibers + 0.5% carbon fibers witnessed the highest flexural toughness, suggesting as a result the optimal fiber mixing ratio of hybrid FRM to improve the strength and flexural toughness at the same time. Moreover, the fracture surface was observed through a scanning electron microscope (SEM) for image analysis of the FRM specimen. These results were of great help for images analysis of hybrid reinforcing fibers in cement matrix.

A Study on the Improvement of Emergency Safety System as Social Protection System - Focusing on Alone Elderly and Severely Person with Disabilities - (사회적 보호체계로서의 응급안전시스템에 대한 개선방안 연구 -독거노인·중증장애인을 중심으로-)

  • Heo, Soo Kyung;Lim, Soo Jeong;Jeong, Jong Hwa
    • 재활복지
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    • v.20 no.4
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    • pp.31-54
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    • 2016
  • The purpose of this study is to explore the improvement method of the emergency safety system as a social protection scheme for the elderly living alone with severe disabilities. The study was conducted by randomly choosing three regions in the metropolitan area (Jongno-gu, Seoul), the small-medium city (Yongin-si, Gyeonggi-do) and the rural area (Geochang-gun, Gyeongsangnam-do) among 17 cities and counties nationwide. The study method is based on the interview form of the structured questionnaires with city officials, county officials, and center operators. According to the results of the interview analysis, it was recognized that there was a need for an emergency safety system for the elderly living alone with severe disabilities. In spite of this necessity, there were problems about publicity for the discovery of victims of the blind spot in welfare, about inconvenience due to frequent malfunctions of the emergency equipment, about insufficient awareness of the security of the emergency officials and about supply and demand of manpower for 24-hour monitoring service. In order to improve the effective operation of the emergency safety system, it was the most urgent issue to formulate the legislation for responding to the crisis management of the vulnerable. A suggestion of this study is to build a safety system in which one-stop service is provided for the realization of compact welfare through the establishment of a MOU for linkage with community organizations, the full inspection of emergency equipment to solve safety equipment malfunction problems and the manpower supplement for 24-hour monitoring.

Investigation of Microbial Communities in Sulculus diversicolor supertexta Through 16S rRNA Sequencing and Antibacterial Monitoring of Harmful Strains (16S rRNA 염기서열 분석을 통한 오분자기(Sulculus diversicolor supertexta)내 미생물 군집 조사 및 인체유해 질병세균에 대한 항균활성 모니터링)

  • Kim, Min-Seon;Lee, Seung-Jong;Heo, Moon-Soo
    • Journal of Life Science
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    • v.28 no.12
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    • pp.1477-1488
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    • 2018
  • This study investigated the muscles, intestines, and gonads of Sulculus diversicolor supertexta to examine the diversity of microbial communities within examples collected from the Jeju Coast. Using different media, initial pure isolation in MA, 1% BHIA, and 1% TSA indicated that the muscles, intestines, and gonads supported more communities, respectively. In analysis of relative similarity with 16s rRNA sequencing, 190 pure colonies were isolated, and further analysis with NBLAST identified 71 species, 39 genera, 25 families, and five phyla. Homogeny with the reference strain was 91-100%. Microbial communities in S. supertexta consisted of gamma and alpha Proteobacteria (48%), Actinobacteria (32.5%), Firmicutes (16.9%), Deinococcus-Thermus (1.3%), and Bacteroides (1.3%). In all tissue, Psychrobacter cibarius in Moraxellaceae was dominant. Alteromonadaceae, Enterobacteriaceae, Pasturellaceae, Moraxellaceae, Rhodobacteraceae, Geminicoccaceae, Dietziaceae, Intrasporangiaceae, Microbacteriaceae, Micrococcaceae, Micromonosporaceae, Streptomycetaceae, Aerococcaceae, Bacillaceae, Paenibacillaceae, Planococcaceae, and Staphylcoccaceae were commonly isolated across all tissues, and Flavobacteriaceae, Corynebacteriaceae, Yesiniaceae, Vibrionaceae, Hahellaceae, Pseudomonadaceae were also identified from the intestines. In microbial monitoring of four harmful bacteria, Streptomyces albus (96%) showed antibacterial activity against all four strains, and Agrococcus baldri (99%) and Psychrobacter nivimaris (99%) presented against E. Coli and E. aerogens. In addition, some strains with low homogeny were isolated and further experiments are therefore required, for example to refine the antimicrobial substances including new strain investigations. These additional experiments would aim to establish generic resources for the microbial communities in S. Supertexta and provide basic data for applied microbiological research.

Mechanical Properties and Impact Resistance Review of Carbon Fiber Reinforced Cement Composites with Different Fiber Contents and Fiber Lengths (섬유혼입률 및 섬유길이 변화에 따른 탄소섬유 보강시멘트 복합재료의 역학적 특성과 내충격성 검토)

  • Heo, Gwang-Hee;Song, Ki-Chang;Park, Jong-Gun;Han, Yoon-Jung;Lim, Cae-Young
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.23 no.4
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    • pp.86-95
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    • 2019
  • Recently, the applications of carbon fiber have been broader than ever when it comes to such industrials as automobiles, ships, aerospace, civil engineering and architecture because of their lightweight-ness and high mechanical properties. This study analyzed mechanical properties and flexural behavior of carbon fiber reinforced cement composites(CFRC) with different fiber contents and fiber lengths, and also impact resistance by natural drop test on mortar specimens was compared and examined. In addition, contents of carbon fiber(CF) were varied by 0.5%, 1.0%, 2.0% and 3.0%. Fiber lengths was used for 6 mm and 12 mm, respectively. As a result of the test, the flow value was very disadvantageous in terms of fluidity due to the carbon fiber ball phenomenon, and the unit weight was slightly reduced. In particular, the compressive strength was decreased with increasing carbon fiber contents. On the other hand, the flexural strength was the highest with 12 mm fiber length and 2% fiber content. As the results of the impact resistance test, the specimens of plain mortar takes about 2~3 times to final fracture, while the specimens of CFRC is somewhat different depending on the increase of the fiber contents. However, when the fiber length is 12 mm and the fiber content is 2%, the impact resistance was the highest.

Development of IoT Searching System Missing Children by utilizing Open Source Hardware (오픈소스 하드웨어를 이용한 IoT 미아찾기 시스템)

  • Heo, Seong-Mu;Kim, Cha-Jong
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2016.10a
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    • pp.277-280
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    • 2016
  • Currently, systems for finding missing children are composed of using communication between a QR code and RFID chip, as the use of a smartphone. However, the current systems for finding missing children have limitations in that children can only be found if there are people in the surrounding area; there is an economic burden on parents required to purchase a smartphone for their children; along with difficulties in finding the missing children without the assistance of those in the surrounding area in critical situations such as a kidnapping, due to the limited duration of the battery life. In order to solve such problems, approaches need to be made from two perspectives: having someone in the surrounding area; and absence of anyone in the surrounding area. This thesis is centered on the development of a IoT (Internet of Things) system for finding missing children that combines two methods, namely, the method of finding missing children without a guardian in the surrounding area -within the limited space in which AP is installed by using a beacon and open source hardware being highlighted as the IoT technology - and the method of finding missing children with the smartphone application in which each individual becomes the Access Point (AP). The Main purpose is to provide accurate information of missing children's location for the 2situations and it is found that the accuracy of smartphones APP is 97.7% and security device AP is 91.1%.

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