• The Korean Journal of Ecology
• /
• v.16 no.1
• /
• pp.39-50
• /
• 1993

The geographical variation of the germination response and the seedling growth of the seed populations of hemistepta lyrata bunge distributed in the southern area of korean peninsula (seoul, yongwol, andong, $Ch^{\prime}angwon, \;Sunch^{\prime}on$)was investigated. Five populations were divided into two principal groups according to the phases of their seed germination. The first group consisting of the seoul and yongwol population was 62% and 64%, respectively, in the final germination percentage of 32%, 24% and 28%, respectively, were Andong, $Ch^{\prime}angwon\;and\;Sunch^{\prime}on$ populations. The seed populations of hemistepta lyrata bunge which hardly germination. the germination of seoul and yongwol populations located at the higher latitudes took plase synchronously in early autumn. On the other hand, andong, $Ch^{\prime}angwon\;and\;Sunch^{\prime}on$ populations located at the lower latitudes showed a tendency to germinata asynchronously in late autumn. the speed of the seedling growth of the populations located at the higher latitudes was greater than that of the populations at the lower latitudes. Therefore the geographical variation in the germination response and the seedling growth of the seed populations of hemistepta lyrate bunge appeared to be an important ecological strategy to maintain their existence in the extreme environmental variations.

• Herbal Formula Science
• /
• v.27 no.1
• /
• pp.7-16
• /
• 2019

Objectives : Hemistepta lyrata Bunge (Bunge) has been used for treating wound, hemorrhage, fever in Korean traditional medicine. Present study investigated anti-inflammatory effect of H. lyrata chloroform extract (HLE) and its molecular mechanism involved. Methods : To assess anti-inflammatory effect of HLE, production of nitric oxide (NO) and expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and pro-inflammatory cytokines were measured on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Moreover, nuclear factor-${\kappa}B$ (NF-${\kappa}B$) signaling pathway was examined to elucidate its molecular mechanism. Results : Pretreatment of HLE inhibited NO production in a concentration dependent manner. HLE also decreased expression of iNOS and COX-2, and alleviated expressions of pro-inflammatory cytokines in LPS-stimulated RAW 264.7 cells. Moreover, HLE pretreatment inhibited phosphorylation of inhibitory-${\kappa}B{\alpha}$ and p65. Conclusions : These results suggest that HLE exhibits anti-inflammatory effect via inhibition of NF-${\kappa}B$.

• Herbal Formula Science
• /
• v.28 no.3
• /
• pp.255-269
• /
• 2020

Objectives : Hemistepta lyrata Bunge (Bunge) is a wild herb that has been used for managing fever and wound in Korean Traditional Medicine. The present study explored the effects of H. lyrata extract on liver X receptor (LXR) α-dependent lipogenic genes in hepatocyte-derived cells. Methods : After HepG2 cells or Huh7 cells were pre-treated with 1-10 ㎍/mL of H. lyrata extract or its fractionated extract for 0.5 h, the cells were subsequently exposed to LXR ligand for 6-24 h. Cell viability, LXR response element (LXRE)-driven luciferase activity, sterol regulatory element binding protein-response element (SREBP-RE)-driven luciferase activity, SREBP-1c expression, and mRNA levels of LXRα and its-dependent target genes were determined. In addition, LC-MS/MS analysis was conducted to explore major compounds in H. lyrata-chloroform fractionated extract #4 (HL-CF4). Results : Of various H. lyrata extracts tested, chloroform extract and its fractionated extract #4, HL-CF4, significantly decreased T0901317-mediated SREBP-1c expression. In addition, HL-CF4 significantly reduced LXRE atransactivation and LXRα mRNA expression without any cytotoxicity. Moreover, HL-CF4 prevented the SREBP-RE-driven luciferase activity and mRNA levels of fatty acid synthase and stearoyl-CoA desaturase-1 induced by T0901317. Results from LC-MS/MS analysis at positive/negative mode indicated that HL-CF4 contained several compounds showing m/z 197.1176 (C₁₁H₁₇O₃), 693.2913/227.1069 (C₃₈H₄₅O₁₂/C₁₅H₁₅O₂), 203.1797 (C₁₅H₂₃), 181.1225 (C₁₁H₁₇O₂), 591.2957 (C₃₅H₄₃O₈), 379.1040 (C₁₈H₁₉O₉), 409.1509 (C₂₀H₂₅O₉), 309.1348 (C₁₆H₂₁O₆), 391.1404 (C₂₀H₂₃O₈), and 669.2924/389.1248 (C₃₆H₄₅O₁₂/C₂₀H₂₁O₈). Conclusion : Based on its inhibition of the LXRα-dependent signaling pathway, H. lyrata chloroform extract and HL-CF4 have prophylactic potentials for managing non-alcoholic fatty liver.

• Journal of Life Science
• /
• v.8 no.2
• /
• pp.131-136
• /
• 1998

Tje dispersion and adaptation of the recently immograted plant, prickly lettuce (Lactuca serriola), were studied. The distribution of prickly lettuce was limited to south of the central districts of the Korean peninsula. The distrbution range is being diffused contimnuously by manual transportation. The 10 selected sites showed typical road side vegetation of an early successional stage; tje quadrats had 90% herbaceous cover, 5% shrub cover, and no tree cover. Dominant species were Kummerowia stipulacea, Ixeris chinensis, Ambrosia artemisiifolia, Lactuca indica, Toungia sonchifolia, Cephalonoplos segetum, Rubus parvifolius, Izeris polycdphala, Hemistepta lyrata, Cercis chinensis Artemisia capillaris. The investigated sites were divided into 4 patterns based on vegetation with high dissimilarity. The presence of mant patterns, despite high livels of differences, indicated that the characteristic prickly lettuce communities were not yet formed.

• Weed & Turfgrass Science
• /
• v.3 no.4
• /
• pp.253-261
• /
• 2014

In this study, we investigated several chemical and physical characteristics of 4 weed seed fibers; Hemistepta lyrata (HEMLY), Imperata cylindrica var. koenigii (IMPCK), Metaplexis japonica (METJA) and Typha latifolia (TYPLA). In chemical composition, there were 74 (TYPLA)-88.5% (METJA) of holocellulose, 17 (IMPCK)-24% (METJA) of lignin, 0.22 (METJA)-4.2% (IMPCK) of ash, 2.2 (HEMLY)-7.8% (IMPCK) of hot water extractives and 0.4 (IMPCK)-6.3% (TYPLA) of solvent extractives. Alpha-cellulose proportion to holocellulose was similar among weed seed fibers as 45-48%. The crystallinity index (CI) of raw seed fibers was 53.2 (TYPLA)-65.9% (HEMLY). However, CI of the chemical treated fibers (EDA fibers) was a little increased and showed 61.1 (IMPCK)-71.8% (METJA). The maximum thermal decomposition temperature (MTDT) of the raw seed fibers were 312, 321.8, 331.5 and $341.6^{\circ}C$ in METJA, TYPLA, HEMLY and IMPCK, respectively. But the MTDT of the EDA fibers were 327, 327, 341.7 and $360.0^{\circ}C$ in HEMLY, TYPLA, METJA and IMPCK, respectively. Taken together, they showed a similar or better characteristics compared to the reported or commercial natural fiber resourses. Accordingly, they seem to be practically applicable as renewable resources for a new natural fibers.

• Korean Journal of Plant Resources
• /
• v.34 no.1
• /
• pp.23-30
• /
• 2021

Hemistepta lyrata Bunge (HL) has been used as a folk remedy to treat cancer, inflammation, bleeding, hemorrhoids and fever, and leaves and young shoots have been used as famine food. Nevertheless, the biological activities and underlying mechanisms of the anti-inflammatory effects remain unclear. In this study, it was undertaken to explore the functions of the aerial part of HL as a suppressor of inflammation by using RAW 264.7 cells. As immune response parameters, the productions of as nitric oxide (NO) and prostaglandin E2 (PGE2), cytokines such tumor necrotic factor (TNF)-α and interleukin (IL)-6 were evaluated. Although the release of TNF-α remained unchanged in HL-treated RAW 264.7 cells, the productions of NO, PGE2 and IL-6 were significantly increased at concentrations with no cytotoxicity. Furthermore, HL significantly attenuated the mitogen-activated protein kinases (MAPK) pathway including decreasing the phosphorylation of the extracellular signal-regulated kinase (ERK1/2) and p38 mitogen-activated protein kinases. Collectively, this study provides evidence that HL inhibits the production of major pro-inflammatory molecules in LPS-stimulated RAW 264.7 cells via suppression of ERK and P38 MAPK signaling pathways. These findings suggest that the beneficial therapeutic effects of HL may be attributed partly to its ability to modulate immune functions in macrophages.

• Applied Biological Chemistry
• /
• v.38 no.6
• /
• pp.584-589
• /
• 1995

To select new useful plants with antibacterial activity, ninety five sample of eighty different species of wild plants were collected, and extracted with methanol. Antibacterial activity of the methanol extracts was tested against Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Vibrio parahaemolyticus. The methanol extracts from Artemisia capillaris, Hemistepta lyrata, Youngia japonica, Prunella vulgaris, Lamium amplexicaule and Juniperus chinensis was effective against all bacterial strains tested, and eight methanol extracts including Ixeris dentata, Gnaphalium affine, Chelidonium majus and Spiraea prunifolia exhibited the antibacterial activity against at least 3 bacterial strains. Methanol extracts from leaf of Syringa vulgaris, Drava nemorosa and clove of Erythronium japonicum showed a selective antibacterial activity against two gram negative bacteria, V. parahaemolyticus, and B. subtilis, respectively. With investigations on antibacterial activity against a certain bacterial strains tested, metahnol extracts from clove of Erythronium japonicum, Spiraea prunifolia, leaf and twig of Camelia japonica, and Drava nemorosa showed strongest activities against B. subtilis, S. aureus, E. coli, and V. parahaemolyticus, respectively. Nine methanol extracts based on the results were successively fractionated with n-hexane, chloroform, ethyl acetate and water portions, which were examined antibacterial activity against B. subtilis and V. parahaemolyticus. Among the all fractions tested, chloroform fractions of Hemistepta lyrata showed strongest antibacterial activity against both B. subtilis (17mm) and V. parahaemolyticus (29 mm). Chloroform fractions of Youngia japonica, n-hexane fractions of Artemisia capillaris, Iexeris dentata and Prunella vulgaris, and ethyl acetate fraction of leaf and twig of Camelia japonica showed relatively a strong antibacterial activity. On the other hand, Juniperus chinensis and Equisetum arvense was distributed to all fractions except for water fraction.

• Journal of Microbiology and Biotechnology
• /
• v.20 no.9
• /
• pp.1339-1347
• /
• 2010

In this study, the identity and distribution of plants and the structure of their associated rhizobacterial communities were examined in an oil-contaminated site. The number of plant species that formed a community or were scattered was 24. The species living in soil highly contaminated with total petroleum hydrocarbon (TPH) (9,000-4,5000 mg/g-soil) were Cynodon dactylon, Persicaria lapathifolia, and Calystegia soldanella (a halophytic species). Among the 24 plant species, the following have been known to be effective for oil removal: C. dactylon, Digitaria sanguinalis, and Cyperus orthostachyus. Denaturing gradient gel electrophoresis (DGGE) profile analysis showed that the following pairs of plant species had highly similar (above 70%) rhizobacterial community structures: Artemisia princeps and Hemistepta lyrata; C. dactylon and P. lapathifolia; Carex kobomugi and Cardamine flexuosa; and Equisetum arvense and D. sanguinalis. The major groups of rhizobacteria were Beta-proteobacteria, Gamma-proteobacteria, Chloroflexi, Actinobacteria, and unknown. Based on DGGE analysis, P. lapathifolia, found for the first time in this study growing in the presence of high TPH, may be a good species for phytoremediation of oil-contaminated soils and in particular, C. soldanella may be useful for soils with high TPH and salt concentrations. Overall, this study suggests that the plant roots, regardless of plant species, may have a similar influence on the bacterial community structure in oil-contaminated soil.

• Biomolecules & Therapeutics
• /
• v.11 no.4
• /
• pp.245-248
• /
• 2003

The purpose of this study was to evaluate tyrosinase inhibitory activity of plant extracts, especially Korean indigenous plants, for the cosmetic use of skin whitening. When 50 plant extracts were tested, the methanol extracts of Agrimonia pilosa, Aster scaber; Dianthus sinensis, Fatsia japonica, Hemistepta lyrata, Lespedeza cuneata, Osmunda japonicum, Pyrvla japvnica, Rodgersia phodophylla and Veratrum grandiforum possessed the considerable tyrosinase inhibitory activity at 3-300 $\mu\textrm{g}$/mL. Especially, L. cuneata, aerial part of O. japonicum and V. gandiforum exhibited the strong inhibition (>50％ inhibition at 300 $\mu\textrm{g}$/mL). In particular, the methanol extract of V. grandiforum and its ethylacetate fraction showed potent inhibition ($IC_{50}$/=30 and 13$\mu\textrm{g}$/mL, respectively), while the reference compound, kojic acid, showed $IC_{50}$/ value of 26$\mu\textrm{g}$/mL. These plant extracts may be used as tyrosinase inhibitors in cosmetics.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2005.05b
• /
• pp.1274-1279
• /
• 2005

The complex vegetation and plant species distributions within riparian corridors influence plant species diversity patterns at both local and regional scales and further reflect both natural and anthropogenic disturbances. Because of these characteristics, riparian zones are often the ecosystem level component that are most sensitive to changes of the surrounding environment; they provide early indications of environmental change and can be viewed as the important source in the watershed. The objectives of this study were two concepts: first, document the composition and dominance of plant communities of riparian areas in the stream, second, compare species composition and temporal diversity between stations in riparian areas of the Ian Stream. The flora was composed to total 158 kinds of the vascular plants as 49 family, 54 genera, 145 species, 12 varieties, 1 forma When the naturalized plant were applied to the recent classification system 280 kinds, the naturalization rate was $10.8\% higher than that of mean value($10.3\%$) of the Korean mountain district. Furthermore, urbanization index (UI) was $6.1\%$. The dominant vegetation communities were distributed in the habitats of three compartments from upstream to downstream. The vegetations were included Phragmites japonica, Salix gracilistyla, S. hulteni and Robinia pseudo-acacia in the riparian area, and Persicaria sieboldii, Stellaria alsine var. undulata, Draba nemorosa var. hebecarpa, Capsella bursa-pastoris, Lepidium apetalum, Bidens frondosa, Trigonotis peduncularis and Hemistepta lyrata in the sandbank or the riparian area, and Equisetum arvense, Humulus japonicus, Persicaria perfoliata, Trifolium repens, Artemisia princeps var. orientalis, Lactuca indica var. laciniata, Avena fatua, Agropyron yesoense, Oenothera odorata, Viola mandshurica, Rumex crispus in banksides, respectively.