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http://dx.doi.org/10.14374/HFS.2019.27.1.7

Anti-inflammatory effect of Hemistepta lyrata Bunge (Bunge) on LPS-induced inflammation in RAW 264.7 cells  

Kim, Jae Kwang (College of Korean Medicine, Daegu Haany University)
Park, Su Young (College of Korean Medicine, Daegu Haany University)
Choi, Hwa Young (College of Korean Medicine, Daegu Haany University)
Jang, Mi Hee (HaniBio Co., Ltd)
Jung, Dae Hwa (HaniBio Co., Ltd)
Kim, Sang Chan (College of Korean Medicine, Daegu Haany University)
Cho, Il Je (College of Korean Medicine, Daegu Haany University)
Publication Information
Herbal Formula Science / v.27, no.1, 2019 , pp. 7-16 More about this Journal
Abstract
Objectives : Hemistepta lyrata Bunge (Bunge) has been used for treating wound, hemorrhage, fever in Korean traditional medicine. Present study investigated anti-inflammatory effect of H. lyrata chloroform extract (HLE) and its molecular mechanism involved. Methods : To assess anti-inflammatory effect of HLE, production of nitric oxide (NO) and expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and pro-inflammatory cytokines were measured on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Moreover, nuclear factor-${\kappa}B$ (NF-${\kappa}B$) signaling pathway was examined to elucidate its molecular mechanism. Results : Pretreatment of HLE inhibited NO production in a concentration dependent manner. HLE also decreased expression of iNOS and COX-2, and alleviated expressions of pro-inflammatory cytokines in LPS-stimulated RAW 264.7 cells. Moreover, HLE pretreatment inhibited phosphorylation of inhibitory-${\kappa}B{\alpha}$ and p65. Conclusions : These results suggest that HLE exhibits anti-inflammatory effect via inhibition of NF-${\kappa}B$.
Keywords
Hemistepta lyrata Bunge (Bunge); RAW 264.7 cells; Inflammation; Nuclear factor-${\kappa}B$ (NF-${\kappa}B$);
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