• Journal of Life Science
• /
• v.14 no.4
• /
• pp.708-715
• /
• 2004

The lactate dehydrogenase (EC 1.1.1.27, LDH) in liver of Lempetra japonica was purified in buffer of affinity chromatography. The liver-specific $C_4$ isozyme of Gadus macrocephalus was purified by heat treatment, affinity chromatography, and DEAE-Sephacel chromatography. The liver-specific $C_4$ isozyme was eluted in a buffer containing NAD+ and was coeluted with $B_4$isozyme in plain buffer of affinity chromagraphy. Liver-specific $C_4$ isozyme in G. macrocephalus was the most thermostable, and$B_4$isozyme was more stable than $A_4$. The LDH in the fraction of pH 7.45 purified from the liver of L. iaponica by chromatofocusing was more inhibited by pyruvate than purified LDH. The optimum pH of the LDH isozyme in the liver of L. japonica was 7.5 and that of liver-specific$C_4$ isozyme was 8.5. The LDH in liver of L. japonica made complexes more with antibody against Coreoperca herzi$A_4$ and liver-specific $C_4$ than with that against eye-specific $C_4$. Therefore, the structure of the LDH in liver of L. japonica might be similarly evolved to that of subunit A and liver-specific $C_4$ isozyme in liver tissue of G. macrocephalus. The evolution rate of subunit C is faster than that of subunit A. LDH in liver of L. japonica has not one isozyme but isozymes and it was also found out to have not only subunit A and B but also subunit C.

• Clean Technology
• /
• v.21 no.4
• /
• pp.271-277
• /
• 2015

A photocatalyst which mixed by the commercialized P25-TiO₂ and a synthesized Ag_xO was used in an appropriate weight ratio to effectively produce hydrogen gas in this study. The Ag_xOs were synthesized with the conventional sol-gel method, and tetramethylammonium hydroxides were added at the synthesis process in order to stabilize the solutions, and then the solutions were heat-treated at the temperatures of -5, 25, and 50 ℃, resulted to obtain the three types of silver oxides. Physicochemical properties of the synthesized Ag_xOs were identified through X-ray diffraction analysis (XRD), scanning emission microscopy (SEM), ultraviolet-visible spectroscopy, and X-ray photoelectron spectroscopy (XPS). In the photolysis results of water/methanol (weight ratio 1:1) solution, the mixture of P25-TiO₂/Ag_xO exhibited a significantly higher hydrogen gases evolution, compared to that of pure P25-TiO₂. Additionally, the addition of H₂O₂ as an supplement oxidant and in Ag_xO synthesized at 50 ℃ improved the hydrogen production efficiency. In particular, the emitted hydrogen gases reached to 13,000 μmol during 8 hours when a mixed catalyst, Ag_xO of 0.1 g and P25-TiO₂ of 0.9 g, were used.

• Korean Journal of Materials Research
• /
• v.3 no.2
• /
• pp.158-165
• /
• 1993

Abstract To investigate the effect of underlying Si on the thermal stability of the TiS$i_2$ film, TiS$i_2$ films obtained by the solid-state reaction of the Ti film on as-deposited or on heat-treated poly-silicon and amorphous-silicon were annealed at 90$0^{\circ}C$ for various times. The poly-Si film was evaluated by XRD, SEM and TEM. The thermal stability of the TiS$i_2$ film was evaluated by measuring the sheet resistance and microstructural evolution during furnace annealing. Agglomeration of the TiSi, film occurred more on amorphous-Si than on poly-Si. The thermal stability of the TiS$i_2$ film was improved by annealing poly-Si. The Si layer crystallized from amorphous-Si has an equiaxed structure with the (111) preferred orientation whereas for as-deposited poly-Si has a columnar structure with the (110) orientation. Better thermal stability of the TiS$i_2$ film can be obtained by the higher surface energy of underlying poly-Si.

• Korean Journal of Mineralogy and Petrology
• /
• v.35 no.3
• /
• pp.199-214
• /
• 2022

The Yeongnam Massif is one of representative basement provinces in the Korean Peninsula, which has experienced high-temperature, low-pressure (HTLP) regional metamorphism and partial melting. Here we reviewed recent developments in Paleoproterozoic (1.87-1.84 Ga) hot orogenesis of the Yeongnam Massif, typified by the granulite-facies metamorphism and partial melting recorded in the HTLP rocks. In particular, spatiotemporal linkage between the metamorphic and magmatic activities, including the Sancheong-Hadong anorthositic magma as a heat source, provides a key to understand the widespread HTLP metamorphism and partial melting in the Yeongnam Massif. Crustal anatexis, resulting from the fluid-present melting and muscovite/biotite dehydration melting, has yielded various types of leucosomes and leucogranites. Zircon and monazite petrochronology, using in-situ U(-Th)-Pb data from the secondary ion mass spectrometry, indicates that the HTLP metamorphism and anatexis lasted over a period of ~15 Ma at ca. 1870-1854 Ma. In addition, a fluid influx event at ca. 1840 Ma was locally recognized by the occurrence of incipient charnockite. Taken together, the Yeongnam Massif preserves a prolonged evolutionary record of the HTLP metamorphism, partial melting, and fluid influx diagnostic for a hot orogen. Such an orogen is linked to the Paleoproterozoic orogeny widespread in the North China Craton, and most likely represents the final phase of crustal evolution in the Columbia/Nuna supercontinent.

• Tunnel and Underground Space
• /
• v.33 no.3
• /
• pp.189-207
• /
• 2023

In the present study, the thermoshearing experiment on a rough rock fracture were modeled using a three-dimensional grain-based distinct element model (GBDEM). The experiment was conducted by the Korea Institute of Construction Technology to investigate the progressive shear failure of fracture under the influence of thermal stress in a critical stress state. The numerical model employs an assembly of multiple polyhedral grains and their interfaces to represent the rock sample, and calculates the coupled thermo-mechanical behavior of the grains (blocks) and the interfaces (contacts) using 3DEC, a DEM code. The primary focus was on simulating the temperature evolution, generation of thermal stress, and shear and normal displacements of the fracture. Two fracture models, namely the mated fracture model and the unmated fracture model, were constructed based on the degree of surface matedness, and their respective behaviors were compared and analyzed. By leveraging the advantage of the DEM, the contact area between the fracture surfaces was continuously monitored during the simulation, enabling an examination of its influence on shear behavior. The numerical results demonstrated distinct differences depending on the degree of the surface matedness at the initial stage. In the mated fracture model, where the surfaces were in almost full contact, the characteristic stages of peak stress and residual stress commonly observed in shear behavior of natural rock joints were reasonably replicated, despite exhibiting discrepancies with the experimental results. The analysis of contact area variation over time confirmed that our numerical model effectively simulated the abrupt normal dilation and shear slip, stress softening phenomenon, and transition to the residual state that occur during the peak stress stage. The unmated fracture model, which closely resembled the experimental specimen, showed qualitative agreement with the experimental observations, including heat transfer characteristics, the progressive shear failure process induced by heating, and the increase in thermal stress. However, there were some mismatches between the numerical and experimental results regarding the onset of fracture slip and the magnitudes of fracture stress and displacement. This research was conducted as part of DECOVALEX-2023 Task G, and we expect the numerical model to be enhanced through continued collaboration with other research teams and validated in further studies.

• Journal of Korean Society of Occupational and Environmental Hygiene
• /
• v.33 no.2
• /
• pp.93-102
• /
• 2023

Objectives: With the evolution of direct reading sensors, it is possible to monitor several substances through telecommunication. However, there are some limitations on the use of direct reading technologies in the Occupational Safety and Health Act in South Korea, which only applies to detector tubes, noise, heat, and carbon monoxides. The number of chemicals and their amount of use have been continuously increasing in South Korea. The Ministry of Employment and Labor (MoEL) has concerns about worker's health because exposure is only covered for about 1.2% of all distributed chemicals. Using a direct reading monitor with photoionization detectors (PID-DRMs), gases and vapors chemicals can be measured. Based on the data, business owners are able to create corrective strategies, provide better working routines, and select correct respiratory equipment. PID-DRMs are less expensive and easier to handle for an owner voluntarily controlling chemicals emitted in the workplace. However, there are several limitations on using these PID-DRMs to the degree that the MoEL has not been able to select a legal monitor. The aim of this study was to review previous studies related to PID-DRMs and identify the characterization and limitation on PID-DRMs. Methods: To search for related studies on PID-DRMs, key words were used including direct reading monitors/instruments and/or photoionization detectors. Through that, four domestic and 15 international studies were reviewed. Results: Studies on PID-DRMs were conducted by chamber (enclosed, dynamic, walk-in) and in the field (experimental environment, actual environment). The concentration of PID-DRMs and charcoal tubes were compared for a single substance or mixture, or within the PID-DRMs. There was a high correlation between the two concentrations, but it did not meet the accuracy criteria (95% confidence interval, within 25%) of the NIOSH technical report (2012). In addition, differences in measured values occurred according to environmental factors (temperature, humidity) and high concentration, and concentration values tended to be underestimated due to contamination of the sensor. As a way to improve the accuracy of PID concentration, it was proposed to use correction factors, charcoal tube-based correction factors, or to calibrate the PID-DRMs in the same environment as the workplace. Conclusions: PID-DRMs can likely be used by business owners for the purpose of voluntarily managing the workplace environment, and it is expected that it will be possible to use them as legal equipment if a PID sensor can be upgraded and the limitations of the sensor (temperature, humidity, high concentration evaluation, sensor pollution) can be overcome in the near future.

• Nuclear Engineering and Technology
• /
• v.56 no.6
• /
• pp.2174-2181
• /
• 2024

In this work, Sr_0.5Zr₂(PO₄)₃-SmPO₄ dual-phase ceramics were prepared via in-situ synthesis process, which is a potential novel nuclear waste form for immobilizing the fission product ⁹⁰Sr and the trivalent actinide radionuclides in high-level waste (HLW). And the preparation technology, microstructure and chemical durability of Sr_0.5Zr₂(PO₄)₃-SmPO₄ dual-phase ceramics were systematically investigated. It was confirmed that the optimum microwave-sintering temperature (1050 ℃) and heat preservation time (1.5 h) is estimated by Archimedes method. Besides, the as-prepared samples that were consisted of strontium zirconium phosphate (SrZP) and monazite showed the remarkable densification, in which the two crystalline phases were intermixed well with each other. Meanwhile, the formation and evolution of microstructure was also consistent with the variational rule of Sr_0.5Zr₂(PO₄)₃/SmPO₄, indicating that there was not mutual reaction during the in-situ synthesis process. The PCT and MCC-1 experimental results demonstrated that the elemental normalized leaching rates of tested samples are all at a low level (LR_Sr ~10^-4 g·m^-2·d^-1, LR_Zr ~10^-8-10^-6 g·m^-2·d^-1, LR_Sm ~10^-7-10^-5 g·m^-2·d^-1 and LR_P ~10^-4 g·m^-2·d^-1). It is indicated that Sr_0.5Zr₂(PO₄)₃-SmPO₄ dual-phase ceramics possesses excellent chemical durability for HLW disposal.

• Korean Journal of Microbiology
• /
• v.47 no.1
• /
• pp.92-96
• /
• 2011

Rhodococcus sp. strain DK17 possesses three megaplasmids (380 kb pDK1, 330 kb pDK2, and 750 kb pDK3). The alkylbenzene-degrading genes (akbABCDEF) are present on pDK2 while the phthalate operons which are duplicated are present on both pDK2 (ophA'B'C'R') and pDK3 (ophABCR). DK17 with an optimal temperature of $30^{\circ}C$ showed no growth at $37^{\circ}C$. When transferred to $30^{\circ}C$, however, the $37^{\circ}C$ culture began to grow immediately, indicating that $37^{\circ}C$ is not lethal but stressful for DK17 growth. In addition, when exposed to $37^{\circ}C$ even for a short time, a part of DK17 cells lost the ability to degrade o-xylene (a model compound of alkylbenzenes). When two hundred colonies were randomly selected for colony PCR for pDK2-specific akbC, ophC', or pDK3-specific ophC, a total of 29 colonies were found to have lost at least one of the three genes. PFGE analysis clearly showed that all the mutants have different megaplasmid profiles from that of DK17 wild type, which are divided into five different cases: Type I (10 mutants, pDK2 loss and acquisition of a new ~700 kb plasmid), Type II (9 mutants, pDK2 loss), Type III (8 mutants, pDK3 loss and acquisition of a new ~400 kb plasmid), Type IV (1 mutant, pDK3 loss), and Type V (1 mutant, pDK2 and pDK3 loss and acquisition of the ~400 kb and ~700 kb plasmids). The above results showing that growth temperature changes can induce physical changes in bacterial genomes suggest that environmental changes in habitats including temperature fluctuations affect significantly the evolution of bacteria.

• Economic and Environmental Geology
• /
• v.34 no.5
• /
• pp.423-435
• /
• 2001

The gold-silver vein deposits in the Mugeug mineralized area are emplaced in late Cretaceous biotite granite associated with the pull-apart type Cretaceous Eumseong basin. Mugeug mine in northern part is composed of multiple veins showing relatively high gold fineness and is characterized by sericitization, chloritization and epidotization. The ore-forming fluids were evolved by dilution and cooling mechanisms at relatively high temperature and salinity (=30$0^{\circ}C$,1~9 equiv. wt. % NaCl) and highly-evolved meteoric water ($\delta$$^{18}$ O;-1.2~3.7$\textperthousand$) and gold mineralization associated with sulfides tormed at temperatures between 260 and 22$0^{\circ}C$ and within sulfur fugacity range of 10$^{-11.5}$ ~ 10$^{-13.5}$ atm. In contrast, Geumwang, Geumbong and Taegueg mines show the low fineness values, in southern part are characterized by increasing tendency of simple and/or stockwork veins and by kaolinitization, silicificatitan, carbonatization and smectitization. These droposits formed at relatively low temperature and salinity (<23$0^{\circ}C$, <3 equiv. wt. % NaCl) from ore-forming fluids containing greater amounts of less-evolved meteoric waters ($\delta$$^{18}$ O;-5.5~4.0$\textperthousand$), and silver mineralization representing various gold-and/or silver-bearing minerals formed at temperatures between 200 and 15$0^{\circ}C$ and from sulfur fugacity range of 10$^{-15}$ ~10$^{-18}$ atm These results imply that mineralization in the Mugueg area formed at shallow-crustal level and categorize these deposits as low-sulfidation epithermal type. The genetic differences between the northern and southern parts reflect the evolution of the hydrothermal system due to a different physicochemical environment from heat source area (Mugeug mine) to marginal area (Taegeum mine) in a geothermal field.

• The Journal of the Petrological Society of Korea
• /
• v.28 no.4
• /
• pp.251-277
• /
• 2019

The Gogunsan Archipelago is composed of two island groups; the first group includes Mal-do, Myeong-do, Gwangdae-do, and Bangchuk-do islands consisting of Neoproterozoic rocks, and the second group includes Yami-do, Sinsi-do, Muneo-do, Jangja-do, and Seonyu-do islands consisting of Cretaceous rocks. The first group mainly consists of the Bangchuk formation which can be divided into two layers; the lower layer was more deformed than the upper layer. The former was intruded by mafic and felsic volcanic rocks formed in the volcanic arc tectonic setting 930-890 Ma and the latter was deposited ca. 825-800 Ma. In these islands, large scale folds with east-west fold axes were beautifully formed; the Maldo island fold was designated as natural monument and large scale beautiful chevron fold was developed on the Gwangdae-do island. In addition, there are unique zebra-shaped outcrop formed by a mixing of basic and acidic magma and Independent Gate shaped outcrop formed by coastal erosion. On the other hand, the Yami-do, Sinsi-do, Muneo-do, Jangja-do and Seonyu-do islands consist of 92-91Ma Cretaceous volcanic rocks and, in Sinsi-do island, the Nanshan formation deposited ca. 92 Ma. These Cretaceous volcanic rocks formed by melting of the continental crust by the heat supplied from the uplifting mantle due to the extension caused by a retreat of subducting ocean slab. Yami-do and Sinsi-do islands are composed of rhyolite. In Yami-do island, bands with vertical joint formed by cooling of the bottom part of the lava, are shown. In Sinsi-do island, large-scale vertical joints formed by cooling of lava flow, were developed. The Jangja-bong of Jangja-do island and Mangju-bong of Seonyu-do island are composed of brecciated rhyolite and formed a ring shaped archipelago contributing to the development of marine culture by providing natural harbor condition. They also provide beautiful views including 'Seonyu 8 views' along with other islands. As mentioned above, the Gogunsan archipelago is rich in geoheritages and associated cultural and historical resources, making it worth as a National Geopark.