• 제목/요약/키워드: HeLa S3

검색결과 173건 처리시간 0.028초

의료 서사와 의료 윤리 (Medical Narrative Texts and Medical Ethics)

  • 최성민
    • 대중서사연구
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    • 제26권3호
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    • pp.291-323
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    • 2020
  • 본 논문은 먼저 펠레그리노의 <의철학의 재탄생>에서 강조하는 현대적 의료 윤리의 가치 변화를 고찰하였다. 펠레그리노는 의료인의 윤리에 대한 인문학적 성찰을 강조해온 철학자이다. 그는 현대 사회의 변화에 따라 의료 윤리도 재정립되어야 한다고 주장한다. 이 논문은 펠레그리노의 주장을 바탕으로, 문학과 대중서사텍스트 속에서 문제적 상황을 주목하였다. 실제로 우리를 위해 의료 윤리가 무엇을 답변해야 하는지를 살펴보려 한 것이다. 의료인들은 철학적 딜레마를 겪거나 현실과 윤리 사이의 갈등을 겪어 왔다. 펠레그리노는 의료인은 질병과의 싸움에 앞서, 환자와의 상호작용을 통해 환자의 고통에 공감하고 그 요구에 응답해야 한다고 주장한다. 이것은 매우 당위적인 선언처럼 보이지만, 문학 작품과 대중서사텍스트 속의 의료인들은 이러한 윤리적 딜레마에 자주 노출된다. 이청준의 소설을 통해 의료인이 환자를 어떻게 '좋음'의 상태로 이끌어야하는 지를 성찰할 수 있으며, 메디컬 드라마들을 통해 대중들이 의료인에게 요구하는 윤리적 행위가 무엇인지를 포착할 수 있다. 코로나19로 전세계가 고통을 받고 있는 지금, 의료인들 또한 큰 고난에 처해 있지만, 동시에 대중의 존경을 받고 윤리적 존재로서의 가치를 높이고 있기도 하다. 의료가 일상의 서사가 된 지금, 우리에게 의료 윤리는 삶을 유지하기 위한 필요조건이 되고 있다. 본 논문을 통해, 의료 윤리의 중요성을 다시 인식하고, 의료 서사 속에서 구체화된 윤리적 문제를 성찰적으로 검토하는 의미를 얻을 수 있을 것이다.

Hericium erinaceum 균사체와 자실체 열수 추출물의 몇몇 In-Vitro 및 In-Vivo 생물활성 (Some In-Vitro and In-Vivo Biological Activities of Hot Water Extracts from Fruit Body and Cultured Mycelium of Hericium erinaceum)

  • 정재현;이광호;이신영
    • KSBB Journal
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    • 제22권1호
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    • pp.22-29
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    • 2007
  • H. erinaceum은 현재 산업적으로 매우 주목되고 있는 기능성 소재들 중의 하나이다. 본 연구에서는 식용은 물론 각종 약리효과를 나타내는 H. erinaceum의 기능성 식품의 제품화 연구 일환으로, 지금까지 체계적으로 연구된 바 없는 H. erinaceum의 액체배양을 시도하고, 생성된 균사체의 생리기능성의 변화를 자실체의 물 추출물과 비교, 조사함으로써 H. erinaceum 액체배양 생성물의 생리기능 효과를 평가, 규명하고자 하였다. H. erinaceum의 자실체 및 균사체 물 추출물은 HeLa (human cervix epitheloid carcinoma; 인간 자궁 상피암세포), Raw264.7 (mouse monocyte macrophage; 생쥐 단핵 대식세포), Jurkat (human acute T cell leukemia, 인간 급성 T 백혈병 세포), KATO3 (human gastric carcinoma 인간 위장 암세포), EL4 (mouse T lymphoma 생쥐 T 림프종 세포), LyD9 (mouse bone marrow plurypotent stem cell; 생쥐 공수유래 간세포) 등 각종 암세포주에 대해 농도 의존적으로 증식 억제 효과를 나타내었다. 자실체 추출물은 균사체 추출물보다 대부분의 암세포 증식의 억제 효과가 컸으며, 가장 높은 암세포 증식 억제효과를 보인 자실체 열수 추출물의 효과는 Raw 264.7 및 EL4 세포주를 사용한 Taxol와의 비교실험 결과, 자실체 농도 0.01$\sim$10 mg/ml에서 자실체 사용량의 1/1000에 상당하는 양의 Taxol 효과와 비슷하였다. 자실체 및 균사체 열수 추출물들은 1 mg/ml 농도에서 복강세포의 nitric oxide 생산 유도능을 나타내었으며, lipopolysaccharides 자극으로 더욱 증가하였다. 반면, 비장세포의 증식유도능 및 interleukin-2 유도능에서는 각 시료 단독으로는 증식 유도 및 interleukin-2 유도능을 나타내지 않았으나 각각 lipopolysaccharide와 concanavalin A의 첨가 및 concanavalin A 자극으로 강한 증식유도능 및 interleukin-2 생산능을 나타내었고, 그 효과는 균사체 추출물에서 자실체 보다 더욱 높았다. 또 각 시료는 골수세포에 대해서도 약간의 증식효과를 보였으며, 복강세포의 탐식능을 나타내었다.

마리 알랭 쿠튀리에가 주창한 성미술(L'Art Sacré) 운동의 건축적 의미와 특성 고찰 (A Study on the Architectural Meaning and Characteristics of L'Art Sacré Movement advocated by Marie Alain Couturie)

  • 반상철;김홍기
    • 한국산학기술학회논문지
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    • 제17권12호
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    • pp.518-531
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    • 2016
  • 본 연구는 현대교회 건축 디자인에 많은 영향을 끼친 도미니크 수도회 신부 마리 알랭 쿠튀리에(Marie Alain Couturie)가 주창한 성미술(L'Art Sacre) 운동의 추진과정과 건축적 특성을 고찰하는데 목적이 있다. 이를 위해 성미술 운동의 전개과정을 분석한 후, 이를 토대로 성미술 운동 속에 내재해 있는 건축적 담론, 특히 쿠튀리에가 주창한 건축개념의 특성이 무엇인가를 분석하고자 하였다. 1차적으로 1936년부터 1954년 사이에 성미술 운동의 기관지 라르 사크레지에 기고 된 쿠튀리에의 글 속에 나타난 언설을 분석하고, 2차적으로 성미술 건축 프로젝트의 현지답사를 통해 성미술 운동의 실체를 파악하였다. 성미술 운동의 대표적인 건축물인 아시성당과 로사리오성당, 롱샹교회, 라투렛수도원, 로스코채플 등 5개의 프로젝트를 분석대상으로 하였다. 본 연구의 결과 쿠튀리에의 건축개념의 특성은 '시적'(Poetic) 분위기의 창출에 기초하고 있음을 파악할 수 있었으며, 재료의 즉물성과 순수함은 모더니즘기의 추상적 사유와 연결되어 있음을 규명할 수 있었다. 쿠튀리에 신부는 르 코르뷔지에를 성미술 운동을 구현할 수 있는 가장 적합한 건축가로 인식하였으며, 이러한 사유는 롱샹 성당과 라투렛 수도원을 탄생시키는 직접적 동인이 되었음을 파악할 수 있었다. 본 연구에서는 20세기 중반 교회건축의 혁신을 일으켰던 성미술 운동의 전개과정과 쿠튀리에 신부가 펼친 건축개념의 분석을 통해 현대교회건축의 공간이미지 구성과 디자인에 지침과 교훈이 될 것으로 사료된다.

주단계(朱丹溪)의 생애(生涯)와 의학사상(醫學思想)에 관한 연구(硏究) (The life and medical idea of Chu, Dan-Gae.(朱 丹溪))

  • 이영원;윤창열
    • 대한한의학원전학회지
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    • 제5권
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    • pp.200-251
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    • 1992
  • As concerned the life and the medical idea of Choo, Tan-Kye(朱丹溪), which it can be summarized as follows by studying. 1. Tan-Kye(丹溪) lived in the end of the won dynasty(元代末期), When the people starved and suffered from a flood-disaster and drought. etc, also the social conditions were in disorder on account of the corrupt ion of politics. And Cheol Kang seong(浙江省), located in the south region of China, has sterile soil and the climate condition humid and heatful. So the south district peoples have very weak constitution. So We can found that his medical idea reflected the phases of the periods and the regional enviornmental situations. 2. For that reason, Tan-Kye(丹溪) rejected the prescription of the "WHa Che Gook Bang(和劑局方)" which was prevalent at that time, in which the the pungent-dried herbs were widly used ; So he persisted in the "Sang Wha Lon(相火論)" and the "Positivity is usually excedeed while the negativity deficient(陽有餘陰不足論)". Then he treated with the drugs to nourish the negativity for the prime object to be applied in the clinic. 3. Tan-Kye(丹溪) refined the follows from the natural law; Heaven is to the positivity(陽) and the Earth is defined the negativity(陰), so the heaven is to the Macro(大) and the earth, micro(小):So the Sun is to the Positivity(陽), the Moon, the Negativity(陰): as to the Sun is always full while the moon always defected too. Therefore the "positivity is always excedeed for that the negativity is deficientalways(陽有餘陰不足)". In Human body, "the negativity energy (陰精) "is hard formed-easily defected(難成易虧)". And the heat(相火) in the body can be moved easily and let the negative energy to leak out. Therefore the more the positivity excedeed, the more the negativity deficient"(陽當有餘陰常不足). 4. He made it expanded the contents of the "Heat(相火)" in the Chapter Woon Chi of the Nae Kyeong(內徑) and discribed, the Life-string of the human body is originated from the movement of the "Heat with unique energy(相火一氣)". And more in human body, it is specifically regulated by the two visceras, Liver and Kidney, and is distributed in the 'Pericardium(心包絡)' 'Tripie Warmer(三焦)' 'Gallbladder(膽)' etc. In the point of his assertion of heat(相火), it is concluded both the physiological and the pathological heat of all. 5. Tan-Kye(丹溪) grew up in the family or the Confucianism. He was instructed the Confucianism(性理學) from Heo-Kyeom(許謙), the fourth diciple of Chu-Ja(朱子), and was received the Yoo Chang Ri(劉 張 李)'s triple doctrine from the La Tae Moo(羅太無), the second disciple of Yoo Wan So(劉完素). So there are much of content of Confucianism(性理學) in his medical thedry, and his theory has succeeded the achievements of the triple study. 6. About the theory of the "positivity is usually excedeed while the negativity deficient"(陽常有餘陰常不足論) of Tan-Kye, it was asserted that the positivity is never sufficient for the vital mainspring, by Chang, Kye-Pin(張介賓) and Lee, Kyoo-Zoon(李奎晙) etc. And for the Heat theory(相火論), eventhough the scholars of postorior generations criicized all of that, there are defect of the content and unification between them. 7. The father of the "Cha Eum Pa(滋陰派), Tan-Kye(丹溪) contributed considerably to the development of the oriental medicine and to the general therapy for the various diseases(一般雜病施治). 8. there are handed down and remained twenty or more of volumes of list of his writings. Among them, except "Kyeok Chi Yeo Ron"(格致餘論), "Kuk Pang Pal Hyeu"(局方發揮), they are reorganized by posteriority. There are Cho, Do-Chin(趙道震). Cho, Ee-Teok(趙以德), Tae, Sa-Gong(戴思恭), Wang Ri(王履) and Yoo, Suk-Yeon(劉淑淵) etc as disciples of his. And Wang Ryoon(王論) and Woo Pak(虞搏) as the admirer of him.

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감초 신품종과 약전 수재 감초 추출물의 항산화 활성 및 암세포 독성 비교 연구 (Antioxidant Activity and Cytotoxicity against Human Cancer Cells of Glycyrrhiza New Varieties : A Comparison with Glycyrrhiza Official Compendia)

  • 김민희;강명훈;이정훈;임강현;안효진;진종식;이종현;장재기;성신;김원남
    • 대한본초학회지
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    • 제36권3호
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    • pp.15-24
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    • 2021
  • Objectives : The Glycyrrhiza new varieties, WONGAM and SINWONGAM, were developed through interspecific cross between Glycyrrhiza glabra and Glycyrrhiza uralensis by the National Institute of Horticultural and Herbal Science, Rural Development Administration in Korea. This in vitro study was undertaken to compare the antioxidant and cytotoxic effects between Glycyrrhiza new varieties (WONGAM and SINWONGAM) and official compendia (Glycyrrhiza glabra and Glycyrrhiza uralensis). Methods : Antioxidant activity was determined by DPPH (1,1-diphenyl-2-picrylhy drazyl), ABTS (2,2-azino-bis (3-rthylbenz-thiazoline-6-sulfonic acid)) diammonium salt, Nitrite radical scavenging assay, and Reducing Power assay. Cytotoxicity was determined by MTT assay and cell morphology was observed by an inverted microscope. Results : The DPPH, ABTS, Nitrite radical scavenging activities and reducing power of Glycyrrhiza glabra, Glycyrrhiza uralensis, WONGAM, and SINWONGAM were evaluated at different concentrations (0, 10, 50, 100, 500, 1000 ㎍/㎖). Glycyrrhiza glabra, Glycyrrhiza uralensis, WONGAM, and SINWONGAM showed similar dose-dependent increase in antioxidant activities. The cytotoxic effects with increasing doses of Glycyrrhiza new varieties and official compendia did not differ in HCT116, HT29, A549, MDA-MB231, PC3, ACHN, and HeLa cells. However, significant difference in cytotoxicity were observed in AGS, MCF7 and Hep3B cells by Glycyrrhiza glabra, Glycyrrhiza uralensis, WONGAM, and SINWONGAM. Conclusions : These results showed that Glycyrrhiza new varieties and official compendia acts as a potent antioxidant. Also, the finding that equivalent cytotoxic potency was observed in a cell dependent manner. Our study suggests that Glycyrrhiza new varieties may offer a wide-variety of health benefits.

찔레 영지버섯(Phellinus ribis) 추출물의 생리활성 (Physiological Activities of Phellinus ribis Extracts)

  • 송재환;이현숙;황진국;정태영;홍성렬;박기문
    • 한국식품과학회지
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    • 제35권4호
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    • pp.690-695
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    • 2003
  • Phellinus ribis(찔레 영지버섯)는 약용버섯으로 일부 항암 및 관절염 치료에 사용되고 있으나 그 활성이 전혀 알려져 있지 않아 1차적으로 40% ethanol 추출물의 생리활성을 확인하였다. 항산화 활성에 있어 nitrosamine의 원인물질인 nitrite 제거활성은 pH 1.2에서 0.5 mg/mL 이하 농도에서는 50% 이하, 그리고 1 mg/mL 처리 시 $64.0{\pm}1.6%$의 제거활성을 나타냈으며, 농도 의존적으로 증가하였다. DNA 손상 및 암, 당뇨, 간경화증, 심혈관질환 등의 원인물질에 관련하여 DPPH radicals의 제거활성은 0.5 mg/mL에서 $62.5{\pm}0.3%$, 1 mg/mL 처리 시 $91.3{\pm}0.8%$로 높게 나타났다. 2.5% linoleic acid의 자동산화에 미치는 항산화 활성을 측정한 결과 0.01 mg/mL 처리 시 p<0.05 수준에서, 0.5 및 1 mg/mL에서는 p<0.001 수준에서 유의성 있게 산화를 억제하였으며, superoxide dismutase 유사활성은 $530{\pm}81\;unit/g$으로 나타났다. 그리고, 항고혈압에 관련된 angiotensin converting enzyme 저해 활성은 $12.0{\pm}1.5%$로 그 활성이 미약하였다. Human 유래 암세포에 대한 세포독성 실험결과 폐암세포인 A549의 경우 100 mg/mL 농도에서도 16%로 나타나 세포독성이 미약하였고, 자궁암 세포인 HeLa의 경우 50 mg/mL에서 45%의 효과를, 위암 세표인 AGS에서는 5 mg/mL 처리 시 24%, 50 mg/mL에서는 76%, 그리고 간암 세포주인 SK-Hep-1의 경우 50 mg/mL에서 42%의 세포독성을 나타내었다. Salmonella typhimurium TA98 및 TA100에 대한 돌연변이원성 시험결과 찔레 영지버섯 추출물은 자연복귀 집락보다 histidine revertant colony 수가 2배 이상 증가하지 않았고, 추출물의 첨가농도를 증가시켜도 histidine revertant colony 수가 증가하지 않아 돌연변이성이 없었다.

Pro-Apoptotic Activity of 4-Isopropyl-2-(1-Phenylethyl) Aniline Isolated from Cordyceps bassiana

  • Kim, Mi Seon;Lee, Yunmi;Sung, Gi-Ho;Kim, Ji Hye;Park, Jae Gwang;Kim, Han Gyung;Baek, Kwang Soo;Cho, Jae Han;Han, Jaegu;Lee, Kang-Hyo;Hong, Sungyoul;Kim, Jong-Hoon;Cho, Jae Youl
    • Biomolecules & Therapeutics
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    • 제23권4호
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    • pp.367-373
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    • 2015
  • Cordyceps species including Cordyceps bassiana are a notable anti-cancer dietary supplement. Previously, we identified several compounds with anti-cancer activity from the butanol fraction (Cb-BF) of Cordyceps bassiana. To expand the structural value of Cb-BF-derived anti-cancer drugs, we employed various chemical moieties to produce a novel Cb-BF-derived chemical derivative, KTH-13-amine-monophenyl [4-isopropyl-2-(1-phenylethyl) aniline (KTH-13-AMP)], which we tested for anti-cancer activity. KTH-13-AMP suppressed the proliferation of MDA-MB-231, HeLa, and C6 glioma cells. KTH-13-AMP also dose-dependently induced morphological changes in C6 glioma cells and time-dependently increased the level of early apoptotic cells stained with annexin V-FITC. Furthermore, the levels of the active full-length forms of caspase-3 and caspase-9 were increased. In contrast, the levels of total forms of caspases-3, caspase-8, caspase-9, and Bcl-2 were decreased in KTH-13-AMP treated-cells. We also confirmed that the phosphorylation of STAT3, Src, and PI3K/p85, which is linked to cell survival, was diminished by treatment with KTH-13-AMP. Therefore, these results strongly suggest that this compound can be used to guide the development of an anti-cancer drug or serve as a lead compound in forming another strong anti-proliferative agent.

퇴원환자의 가정간호요구와 가정간호사업의 효과 분석 - 일 종합병원을 중심으로 (A Study of Home Care Needs of Patients at Discharge and Effects of Home Care -Centered on Patients Discharged from a Rural General Hospilal-)

  • 최연순;김대현;서미혜;김조자;강규숙
    • 대한간호
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    • 제31권4호
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    • pp.77-99
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    • 1992
  • The study was carried out at W. hospital, an affiliated hospital of Y university, involved a total of 163 patients who were discharged from the hospital between May 1990 und March 199J. Data collection was twice, just prior to discharge and a minimum of three months post discharge. Thirty patients who lived within a hour travel time of the hospital received home care during the three months post discharge. Nursing diagnoses and nursing interventions For these patients were analyzed in this study. The results of the study are summarized as follows : 1. Discharge needs for the subjects of the study were analyzed using Gordon's eleven Functional categories and it was found that 48.3% of the total sample had identified nursing needs. Of these, the needs most frequently identified were in the categories of sexuality, 79.3 %, health perception, 68.2 % self concept, 62.5 %, and sleep and rest 62.5 %. Looking ut j he nursing diagnosis that were made for the 30 patients receiving home care, the following diagnoses were the most frequently given; alteration in sexual pattern 79.3%, alterations in health maintenance, 72.6%, alteration in comfort, 68.0%, depression, 64.0%, noncompliance with diet therapy, 6.3.7%, alteration in self concept, 55.6%, and alteration in sleep pattern, 53%. 2. In looking at the effects of home nursing care as demonstrated by changes in the functional categories over the three month period, it was Found that of the 11 functional categories, the need level for health perception, nutrition, activity and self concept decreased slightly over the three month period. On the average sleep patterns improved, but restfulness was slightly less and bowel elimination patterns improved but satisfaction with urinary elimination was slightly less. On the other hand, role enactment, sexuality, stress management and spirituality decreased slightly. The only results that were statistically significant at the 0.05 level were improvement. in digestion and decrease in pain. No statistically significant changes were found in ability related to ADL, the total ADL Score at discharge was $19.78{\pm}8.234, and after 3 months $19.01{\pm}8.12$. Considering that a majority of the patients were over 60 years of age and that many had brain or spinal cord injuries, the fact that their ADL ability did nor deteriorate after discharge can be interpreted as related to a positive impact by the home health care nurses. Similarly there was a slight be not statistically significant decrease in the quality of life scores between the two lest times(l47.83 at discharge and 113.02 at the three month period). Again, when the chronic nature of thee problems facing these patients is considered this maintenance of quality of life can be interpreted as a positive impact by the home health care nurses. 3. One of the home care nursing activities was diagnosis. For this activity it was found that for nine functional health categories(sexuality and spirituality excepted) there were 20 nursing diagnoses. The most frequent were noncompliance, alteration in skin integrity both actual and potential, and impaired physical mobility in that order. 4. Delivery of home health care by the home health nurses included the following nursing activities; assessment, patient education, demonstration of care activities, counselling, direct care to the patient and referrals. Direct care included changing dressings, bladder irrigations, changing Foley catheters, measurement of residual urine, perineal care, position change, back care, oral hygiene, exercise and massage of motion exercises, cleansing enemas, tracheostomy suctioning and tracheostomy care, care of dentures, applications of heat and other similar nursing activities. In conclusion almost 50% of (he sample indicated a need for continued nursing care at the time of discharge and for the patients in the sample who received home care there was a slight decrease in nursing needs but while the patients had chronic and debilitation problems there was ill decrease in ADL abilities or in quality of life. Further study needs Lo be done La increase the reliability and validity of the tool that was used to measure home health care needs. It is also recommended that study by done using a randomized sampling with a control group to compare patients who receive home care with those who do not.

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Human Immunodeficiency Virus-1 Tat 단백에 의한 인간 CD99유전자의 조절기전에 대한 연구 (Human Immunodeficiency Virus-l Tat Positively Regulates the Human CD99 Gene via DNA Demethylation)

  • 이유진;김예리;이미경;이임순
    • 미생물학회지
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    • 제44권4호
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    • pp.277-281
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    • 2008
  • HIV에 감염된 환자의 경우 다양한 종류의 암이 발생하는 것으로 알려져 있다. 이러한 암종의 높은 발생률의 원인으로, 감염에 의한 면역세포의 감소 및 결핍과 같은 간접적인 이유 뿐 아니라, HIV 바이러스 단백질의 발현이 직접적으로 병의 발생에 관여한다는 보고가 있다. 본 연구에서는 HIV 환자에서 높게 나타나는 암의 발생에 대한 기전을 이해하기 위하여 HIV-1 Tat 유전자와, 다수의 암 발생과 관련이 있는 세포막단백 CD99와의 관계를 규명하였다. 먼저 CD99의 발현에 미치는 Tat의 영향을 알아보기 위하여 HIV-1 Tat 발현 안정화 세포주를 확립하고 Tat 단백에 의한 CD99 유전자의 발현 양상 변화를 분석하였다. 실험결과 Tat의 발현에 의하여 CD99 유전자의 발현이 활성화되는 것이 관찰되었으며 이와 반대로 STAT3의 발현은 낮아졌다. CD99 프로모터는 CpG 함량이 높기 때문에 Tat 단백이 DNA 메칠화를 통해서 CD99 유전자의 발현을 조절하는지 확인하기 위하여 methylation specific PCR을 수행하였고 Tat의 발현이 높은 곳에서 특이적으로 CD99 프로모터 부위가 탈메칠화되는 것을 발견하였다. Tat 발현 세포에서만 특이적인 발현 차이를 보이는 유전자 분석을 위한 Differentially Expressed Gene keratin 17과 collagen, type IV 증가됨이 확인되었다. 위의 결과는 HIV Tat 단백이 직접 세포 단백들을 조절하여 암을 발생시킬 수 있다는 보고를 뒷받침한다.

'아유르베다'($\bar{A}yurveda$)의 의경(醫經)에 관한 연구 (A Study of The Medical Classics in the '$\bar{A}yurveda$')

  • 김기욱;박현국;서지영
    • 대한한의학원전학회지
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    • 제20권4호
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    • pp.91-117
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    • 2007
  • Through a simple study of the medical classics in the '$\bar{A}yurveda$', we have summarized them as follows. 1) Traditional Indian medicine started in the Ganges river area at about 1500 B. C. E. and traces of medical science can be found in the "Rigveda" and "Atharvaveda". 2) The "Charaka" and "$Su\acute{s}hruta$(妙聞集)", ancient texts from India, are not the work of one person, but the result of the work and errors of different doctors and philosophers. Due to the lack of historical records, the time of Charaka or $Su\acute{s}hruta$(妙聞)s' lives are not exactly known. So the completion of the "Charaka" is estimated at 1st${\sim}$2nd century C. E. in northwestern India, and the "$Su\acute{s}hruta$" is estimated to have been completed in 3rd${\sim}$4th century C. E. in central India. Also, the "Charaka" contains details on internal medicine, while the "$Su\acute{s}hruta$" contains more details on surgery by comparison. 3) '$V\bar{a}gbhata$', one of the revered Vriddha Trayi(triad of the ancients, 三醫聖) of the '$\bar{A}yurveda$', lived and worked in about the 7th century and wrote the "$A\d{s}\d{t}\bar{a}nga$ $A\d{s}\d{t}\bar{a}nga$ $h\d{r}daya$ $sa\d{m}hit\bar{a}$ $samhit\bar{a}$(八支集)" and "$A\d{s}\d{t}\bar{a}nga$ Sangraha $samhit\bar{a}$(八心集)", where he tried to compromise and unify the "Charaka" and "$Su\acute{s}hruta$". The "$A\d{s}\d{t}\bar{a}nga$ Sangraha $samhit\bar{a}$" was translated into Tibetan and Arabic at about the 8th${\sim}$9th century, and if we generalize the medicinal plants recorded in each the "Charaka", "$Su\acute{s}hruta$" and the "$A\d{s}\d{t}\bar{a}nga$ Sangraha $samhit\bar{a}$", there are 240, 370, 240 types each. 4) The 'Madhava' focused on one of the subjects of Indian medicine, '$Nid\bar{a}na$' ie meaning "the cause of diseases(病因論)", and in one of the copies found by Bower in 4th century C. E. we can see that it uses prescriptions from the "BuHaLaJi(布哈拉集)", "Charaka", "$Su\acute{s}hruta$". 5) According to the "Charaka", there were 8 branches of ancient medicine in India : treatment of the body(kayacikitsa), special surgery(salakya), removal of alien substances(salyapahartka), treatment of poison or mis-combined medicines(visagaravairodhikaprasamana), the study of ghosts(bhutavidya), pediatrics(kaumarabhrtya), perennial youth and long life(rasayana), and the strengthening of the essence of the body(vajikarana). 6) The '$\bar{A}yurveda$', which originated from ancient experience, was recorded in Sanskrit, which was a theorization of knowledge, and also was written in verses to make memorizing easy, and made medicine the exclusive possession of the Brahmin. The first annotations were 1060 for the "Charaka", 1200 for the "$Su\acute{s}hruta$", 1150 for the "$A\d{s}\d{t}\bar{a}nga$ Sangraha $samhit\bar{a}$", and 1100 for the "$Nid\bar{a}na$", The use of various mineral medicines in the "Charaka" or the use of mercury as internal medicine in the "$A\d{s}\d{t}\bar{a}nga$ Sangraha $samhit\bar{a}$", and the palpation of the pulse for diagnosing in the '$\bar{A}yurveda$' and 'XiZhang(西藏)' medicine are similar to TCM's pulse diagnostics. The coexistence with Arabian 'Unani' medicine, compromise with western medicine and the reactionism trend restored the '$\bar{A}yurveda$' today. 7) The "Charaka" is a book inclined to internal medicine that investigates the origin of human disease which used the dualism of the 'Samkhya', the natural philosophy of the 'Vaisesika' and the logic of the 'Nyaya' in medical theories, and its structure has 16 syllables per line, 2 lines per poem and is recorded in poetry and prose. Also, the "Charaka" can be summarized into the introduction, cause, judgement, body, sensory organs, treatment, pharmaceuticals, and end, and can be seen as a work that strongly reflects the moral code of Brahmin and Aryans. 8) In extracting bloody pus, the "Charaka" introduces a 'sharp tool' bloodletting treatment, while the "$Su\scute{s}hruta$" introduces many surgical methods such as the use of gourd dippers, horns, sucking the blood with leeches. Also the "$Su\acute{s}hruta$" has 19 chapters specializing in ophthalmology, and shows 76 types of eye diseases and their treatments. 9) Since anatomy did not develop in Indian medicine, the inner structure of the human body was not well known. The only exception is 'GuXiangXue(骨相學)' which developed from 'Atharvaveda' times and the "$A\d{s}\d{t}\bar{a}nga$ Sangraha $samhit\bar{a}$". In the "$A\d{s}\d{t}\bar{a}nga$ Sangraha $samhit\bar{a}$"'s 'ShenTiLun(身體論)' there is a thorough listing of the development of a child from pregnancy to birth. The '$\bar{A}yurveda$' is not just an ancient traditional medical system but is being called alternative medicine in the west because of its ability to supplement western medicine and, as its effects are being proved scientifically it is gaining attention worldwide. We would like to say that what we have researched is just a small fragment and a limited view, and would like to correct and supplement any insufficient parts through more research of new records.

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