The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Antioxidant Activity and Cytotoxicity against Human Cancer Cells of Glycyrrhiza New Varieties : A Comparison with Glycyrrhiza Official Compendia

감초 신품종과 약전 수재 감초 추출물의 항산화 활성 및 암세포 독성 비교 연구

  • Kim, Minhee (Division of Pharmacology, College of Korean Medicine, Semyung University) ;
  • Kang, Myunghoon (Division of Pharmacology, College of Korean Medicine, Semyung University) ;
  • Lee, Jeonghoon (Herbal Crop Research Division, NIHHS, RDA) ;
  • Leem, Kang-Hyun (Division of Pharmacology, College of Korean Medicine, Semyung University) ;
  • An, Hyo-Jin (Department of Pharmacology, College of Korean Medicine, Sangji University) ;
  • Jin, Jong-Sik (Department of Oriental Medicine Resources, Jeonbuk National University) ;
  • Lee, Jong-Hyun (Department of Pharmacy, College of Pharmacy, Dongduk Women's University) ;
  • Chang, Jaeki (Crop Production & Physiology Division, NICS, RDA) ;
  • Seong, Shin (Soram Korean Medicine Hospital) ;
  • Kim, Wonnam (Cnh Center for Cancer Research)
  • 김민희 (세명대학교 한의과대학 본초방제학교실) ;
  • 강명훈 (세명대학교 한의과대학 본초방제학교실) ;
  • 이정훈 (농촌진흥청 국립원예특작과학원 약용작물과) ;
  • 임강현 (세명대학교 한의과대학 본초방제학교실) ;
  • 안효진 (상지대학교 한의과대학 약리학교실) ;
  • 진종식 (전북대학교 환경생명자원대학 한약자원학과) ;
  • 이종현 (동덕여자대학교 약학대학 약학과) ;
  • 장재기 (농촌진흥청 국립식량과학원 작물재배생리과) ;
  • 성신 (소람한방병원) ;
  • 김원남 (Cnh 암의학연구소)
  • Received : 2021.04.12
  • Accepted : 2021.05.25
  • Published : 2021.05.30
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Abstract

Objectives : The Glycyrrhiza new varieties, WONGAM and SINWONGAM, were developed through interspecific cross between Glycyrrhiza glabra and Glycyrrhiza uralensis by the National Institute of Horticultural and Herbal Science, Rural Development Administration in Korea. This in vitro study was undertaken to compare the antioxidant and cytotoxic effects between Glycyrrhiza new varieties (WONGAM and SINWONGAM) and official compendia (Glycyrrhiza glabra and Glycyrrhiza uralensis). Methods : Antioxidant activity was determined by DPPH (1,1-diphenyl-2-picrylhy drazyl), ABTS (2,2-azino-bis (3-rthylbenz-thiazoline-6-sulfonic acid)) diammonium salt, Nitrite radical scavenging assay, and Reducing Power assay. Cytotoxicity was determined by MTT assay and cell morphology was observed by an inverted microscope. Results : The DPPH, ABTS, Nitrite radical scavenging activities and reducing power of Glycyrrhiza glabra, Glycyrrhiza uralensis, WONGAM, and SINWONGAM were evaluated at different concentrations (0, 10, 50, 100, 500, 1000 ㎍/㎖). Glycyrrhiza glabra, Glycyrrhiza uralensis, WONGAM, and SINWONGAM showed similar dose-dependent increase in antioxidant activities. The cytotoxic effects with increasing doses of Glycyrrhiza new varieties and official compendia did not differ in HCT116, HT29, A549, MDA-MB231, PC3, ACHN, and HeLa cells. However, significant difference in cytotoxicity were observed in AGS, MCF7 and Hep3B cells by Glycyrrhiza glabra, Glycyrrhiza uralensis, WONGAM, and SINWONGAM. Conclusions : These results showed that Glycyrrhiza new varieties and official compendia acts as a potent antioxidant. Also, the finding that equivalent cytotoxic potency was observed in a cell dependent manner. Our study suggests that Glycyrrhiza new varieties may offer a wide-variety of health benefits.

Keywords

Acknowledgement

본 논문은 농촌진흥청 연구사업(세부과제번호: PJ01424602)의 지원에 의해 이루어진 것임.

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