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http://dx.doi.org/10.4062/biomolther.2015.021

Pro-Apoptotic Activity of 4-Isopropyl-2-(1-Phenylethyl) Aniline Isolated from Cordyceps bassiana  

Kim, Mi Seon (Department of Genetic Engineering, Sungkyunkwan University)
Lee, Yunmi (Department of Chemistry, Kwangwoon University)
Sung, Gi-Ho (Institute for Bio-Medical Convergence, International St. Mary's Hospital and College of Medicine, Catholic Kwandong University)
Kim, Ji Hye (Department of Genetic Engineering, Sungkyunkwan University)
Park, Jae Gwang (Department of Genetic Engineering, Sungkyunkwan University)
Kim, Han Gyung (Department of Genetic Engineering, Sungkyunkwan University)
Baek, Kwang Soo (Department of Genetic Engineering, Sungkyunkwan University)
Cho, Jae Han (Mushroom Research Division, Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA)
Han, Jaegu (Mushroom Research Division, Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA)
Lee, Kang-Hyo (Mushroom Research Division, Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, RDA)
Hong, Sungyoul (Department of Genetic Engineering, Sungkyunkwan University)
Kim, Jong-Hoon (Department of Veterinary Physiology, College of Veterinary Medicine, Biosafety Research Institute, Chonbuk National University)
Cho, Jae Youl (Department of Genetic Engineering, Sungkyunkwan University)
Publication Information
Biomolecules & Therapeutics / v.23, no.4, 2015 , pp. 367-373 More about this Journal
Abstract
Cordyceps species including Cordyceps bassiana are a notable anti-cancer dietary supplement. Previously, we identified several compounds with anti-cancer activity from the butanol fraction (Cb-BF) of Cordyceps bassiana. To expand the structural value of Cb-BF-derived anti-cancer drugs, we employed various chemical moieties to produce a novel Cb-BF-derived chemical derivative, KTH-13-amine-monophenyl [4-isopropyl-2-(1-phenylethyl) aniline (KTH-13-AMP)], which we tested for anti-cancer activity. KTH-13-AMP suppressed the proliferation of MDA-MB-231, HeLa, and C6 glioma cells. KTH-13-AMP also dose-dependently induced morphological changes in C6 glioma cells and time-dependently increased the level of early apoptotic cells stained with annexin V-FITC. Furthermore, the levels of the active full-length forms of caspase-3 and caspase-9 were increased. In contrast, the levels of total forms of caspases-3, caspase-8, caspase-9, and Bcl-2 were decreased in KTH-13-AMP treated-cells. We also confirmed that the phosphorylation of STAT3, Src, and PI3K/p85, which is linked to cell survival, was diminished by treatment with KTH-13-AMP. Therefore, these results strongly suggest that this compound can be used to guide the development of an anti-cancer drug or serve as a lead compound in forming another strong anti-proliferative agent.
Keywords
Cordyceps bassiana; KTH-13-amine-monophenyl; Anti-cancer activity; Proliferation; Apoptosis; Cell survival;
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Times Cited By KSCI : 6  (Citation Analysis)
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