• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.433-433
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• 2000

The monogenean skin parasite, Entobdella hippoglossi has commonly be found broodstock of the Atantic halibut. Heavy infestation of the parasite appears to be serious problem to the halibut farming industry. However, because the behavior of host, research on this parasite is not yet be carried out. The present study, therefore, was investigated the oncomiracidium of Entobdella hippoglossi to the basic step for solving this problem. (omitted)

• Journal of the Korean Society of Food Science and Nutrition
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• v.28 no.3
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• pp.691-697
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• 1999

The pharmacological effects of hydrocooked(110oC, 5 hours) extracts of Bastard halibut have been investigated. All of the hydrocooked extracts showed the measurable contractile effect on the isolated rat duodenum and decreased the normal blood pressure in anesthetized rat. The hydrocooked extracts also exhibited a dose dependent relaxation on the isolated rat aorta precontracted with phenylephrine. Only RM 60 fraction of these extracts had the cytotoxic effect against MCF7 cell(human breast adenocarcinoma cell line), but the other fractions showed neither antibacterial activity nor antitumor activity. Although fish extracts fed group of rat maintained their original body weight, there were no notable changes in the hematological parameters, except that the levels of high density lipoprotein was significantly increased. These results suggest that the hydrocooked extracts of bastard halibut may contain a variety of bioactive materials.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.36 no.4
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• pp.358-364
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• 2003

Gross energy intake fecal, nitrogen excretory and metabolized energy of snotted halibut juvenile, Eopsetta grigorjewi, fed on Tigriopus iaponicus (Group A) and the enriched Artemia nauplii (Group B), were quantitatively investigated and represented as balance equations of energy and material. Absorption efficiency (AE), gross conversion efficiency $(K_1)$ and net conversion efnciency $(K_2)$ were also estimated to understand the changes in efficiency by the prey Gross energy intake, fecal, nitrogen excretory, metabolized and growth energy of Group A were $2.790\pm0.247,\;0.495\pm0.046,\;0.018\pm0.003,\;0.214\pm0.047\;cal\;mg^{-1}\;day^{-1}$ and $2.063\;cal\;mg^{-1}\;day^{-1},$ respectively. The same parameters of Group B were $4.801\pm0.165,\;0.579\pm0.031,\;0.055\pm0.010,\;0.306\pm0.048\;cal\;mg^{-1}\;day^{-1}$ and $3.861\;cal\;mg^{-1}\;day^{-1},$ respectively. Energy budget of Group A and B were represented as 100 C= 7.67 M+17.7 F+0.65 U+73.94 G and 100C=6.37 M+ 12.1 F+1.15 U+80.42 G, respectively, where C, M, F, U, and G represent gross energy intake, metabolized energy, fecal energy, nitrogen excretory (non-fecal) energy and growth energy, respectively. AEs of Croup A and B were $81.52\pm1.89,\;86.79\pm0.70\%,$ respectively, with significant difference at p=0.01 level. However, $K_1\;and K_2$ of Group A and B showed no significant difference at p=0.01 level, with $74.21\pm6.57,\;80.48\pm2.76\%\;and\;91.17\pm7.26,\;92.74\pm2.69\%$ respectively. These results suggest that T. japonicus is a possible substitute for Artemia nauplii for the snotted halibut juvenile.

• Korean Journal of Ichthyology
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• v.23 no.1
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• pp.30-36
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• 2011

Age and growth of shotted halibut Eopsetta grigorjewi were estimated using right sagittal otoliths of 389 fish specimens from February 2004 to January 2005 in the East China Sea. Examination of outer margins of the otolith showed that the opaque zone was formed once a year and annual rings were formed from December to March. The age of specimens examined ranged from 3 to 5 years. Shotted halibut begin spawning in February and show a peak in March. Length and weight relationships showed no significant difference between females and males (P>0.05), and can be expressed as TW=$0.5091{\times}10^{-2}TL^{3,222}(r^2=0.92)$. Estimated von Bertalanffy growth curve was $L_t=46.58(1-e^{-0.14(1+1.32)})$.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.48 no.2
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• pp.178-186
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• 2015

Protease inhibitors (PI) of trypsin and papain as target proteases from the roe of bastard halibut Paralichthys olivaceus were fractionated out using ammonium sulfate precipitation (A), DEAE 650M anion exchange chromatography (D), and Sephacryl S-300 gel filtration (S). The recovery percentages of the fractions with the strongest inhibitory activity for each fractionation method were 13% for the A4 fraction, 21.2% for the D3 fraction, and 21.3% for the S2 fraction, with specific inhibitory activities of the fractions toward trypsin and casein of 168, 139, and 218 U/mg, respectively, while no inhibition of papain was observed. The $IC_{50}$ for the trypsin-specific substrate $N{\alpha}$-benzoyl-$\small{L}$-arginine-p-nitroanilide (BAPNA) was 0.65, 1.55, 2.26, and 2.85 mg/mL for the A4, S2, A3, and D3 fractions, respectively. These results suggest that chromatographic fractionation methods (D and S) based on the molecular mass and charge of the protein were more effective at fractionating PI than was ammonium sulfate precipitation based on protein solubility, and that the bastard halibut roe extract acts as a serine protease inhibitor. Therefore, the PI fraction from fish roe might be useful for inhibiting proteases in foodstuffs, and could constitute an alternative food-grade inhibitor for the surimi industry.

• Preventive Nutrition and Food Science
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• v.2 no.4
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• pp.321-327
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• 1997

High Temperature-cooking conditions of cultured fishes(loach, crucian carp, bastard halibut, and jacopever) were optimized by response surface methodology(RSM), and plastein products were prepared using enzymatic hydrolysis. Four models were proposed with regard to effects of time(t), temperature(T), and water/fish meat (w/f) ratio on the amount of 0.3M TCA soluble fractions. The model coefficients were ranged from p<0.0001 for jacopever to p<0.0433 for bastared halibut. Cooking conditions for 60% hydrolysis were optimized at 1) 14$0^{\circ}C$ except for crucian carp(136$^{\circ}C$); 2) 10.08 hours(loach), 7.25 hours(crucian carp), 9.85 hours(ba-stard harlibut), and 9.37 hours(iacopever); 3) 1:1(w/f) ratio except for the crucian carp(1.1:1). When protein hydrolyzates were employed for the plastein synthesis, optimum plastein-reaction conditions were determined to be pH 9.0 with chymotrypsin for the loach and crucian carp hydrolyzates, pH 9.0 with papain for the bastard halibut hydrolyzate, and pH 11.0 with trypsin for the jacopever hydrolyzate. Plastein reaction could be performed in water at concentration up to 20%(w/f).

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.47 no.3
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• pp.194-202
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• 2011

Maturation and spawning of the Shotted halibut, Eopsetta grigorjewi was investigated based on the samples captured in South Korean waters from January 2008 to December 2009. Gonadosomatic index began to increase in December, and reached maximum between January to March. After spawning it began to decrease from May. Reproductive season was estimated to January-April, with peak in February. Fecundity was proportional to the size of the female, with the clutch size varying from 170,000 eggs in the smallest female (total length, 28.9cm) to 1,300,000 eggs in the largest (total length, 41.5cm). Size at 50% sexual maturity (TL50), determined from mature females, was 28.8cm. Annual reproductive cycles of this species could be divided into six successive stages; immature stage (May-October), nucleolus stage (November-January), yolk vesicle stage (January-February), vitellogenic and ripe stage (January-April) and spent stage (April-May).

• Journal of Fisheries and Marine Sciences Education
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• v.15 no.2
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• pp.135-144
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• 2003

1. The appearance of fisheries animals and plants of investigation areas were fishes, 20 species; algae, 9 species; molluska, 6 species; arthropod, 12 species; echinoderm, 5 species by confirmed appearance species. 2. The C.P.U.E by trap(1pcs of trap) were 3.95 species and weight of 2,098g, mainly catching species are greenling and jacopever(94.16%). The C.P.U.E by long liner(long liner 1coil, 100pcs) were 11.7species and weight of 6,418g, It occupied jacopever(35.63%) greenling(20.78%), bastard halibut(20.31%). The C.P.U.E of appearance species gill net(ipcs of gill net) were 5.05species and weight of 3,050g, It occupied jacopever(39.63%) greenling(29.83%), bastard halibut(17.47%). 3. The results of comparative analysis and natural species and stocked species, in case of bastard halibut, jacopever species were occupied species(83.33~92%), weight(86.86~96%). The stocked species were occupied species of 8.00~16.67%, weight of 4.00~13.14%. Provided the stocked projets were enacted effectively in future. The density of dwelling of stocked species were increase. Also, it will be contribute to the increase of fishermens income.

• Journal of Food Hygiene and Safety
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• v.14 no.1
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• pp.27-33
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• 1999

Phytic acid, making up 1~5% of the composition of many plant seeds and cereals, is known to form iron-chelates and inhibit lipid peroxidation. Thiobarbituric acid reactive substances (TBARS), as an indication of lipid peroxidation, were measured in beef round, chicken breast, pork loin, and halibut muscle after the meats were stored for 0, 1, 3, 5, and 7 days at various temperatures [frozen (~2$0^{\circ}C$), refrigerator (4$^{\circ}C$), and room temperature ($25^{\circ}C$)]. Phytic acid effectively inhibited lipid peroxidation in beef round, chicken breast, halibut, and pork loin muscle (p<0.05). The inhibitory effect of phytic acid was dependent on concentration, storage time, and temperature. At frozen temperature, the inhibitory effect of phytic acid was minimal, whereas at room temperature, the inhibitory effect of phytic acid was maximal, probably due to the variation of the control TBARS values. At the concentration of 10 mM, phytic acid completely inhibited lipid peroxidation in all the muscle foods by maintaining TBARS values close to the level of the controls, regardless of storage time or temperature (p<0.05). The rate of lipid peroxidation was the highest in beef round muscle, although they had a close TBARS value at 0 day. Addition of phytic acid to lipid-containing foods such as meats, fish meal pastes, and canned seafoods may prevent lipid peroxidation, resulting in improvement of the sensory quality of many foods and prolonged shelf-life.

• Journal of fish pathology
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• v.25 no.3
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• pp.243-247
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• 2012

Histopathological observations on a epidermal papilloma of wild red halibut (Hippogossoides dubius) caught at the area around Echung Island and Gogunsan Islands were done. The papilloma formed on the dorsal fin was the same color with the body on the ocular side but black on the non-ocular side. Under light microscopy, the tumor on the skin was very similar to epithelial papilloma supported by connective tissue. X-cell-like cells in the tumors were large and eosinophilic with a centrally-located, large nucleus. But the characteristic large nucleolus in the nucleus of the x-like cells was indistinct clear in this specimen because about 6-9 hours have passed after catching the fish and autolysis have occurred.