• KSBB Journal
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• 제13권6호
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• pp.662-668
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• 1998

Viruses belonging to the Hantavirus genus cause two acute severe illness in humans, i.e., Haemorrhagic Fever with Renal Syndrome (HFRS) and Hantavirus Pulmonary Syndrome(HPS). Among them, Hantaan virus is one of the most important viruses causing HFRS. Recombinant expression vectors, pKK-NP and pET-NP, with Hantaan viral nucleocapsid gene were constructed, and used to transform Eschericia coli BL21(DE3). Stability of the vectors in the host strain, and effects of some environmental conditions on the expression of nucleocapsid gene were studied. Expression vector, pKK-NP, was very unstable, and the expression level of nucleocapsid gene was very low compared to that of pET-NP. BL21(pET-NP) produced about 100 mg of N protein per liter of culture broth. Induction time did not show any significant difference on the expression level of nucleocapsid gen and cell growth. BL21(pET-NP) culture at 35$^{\circ}C$ showed a little higher expression level than at 30$^{\circ}C$ during growth phase, but reached to the same level at stationary phase. Total expression level was proportional to supplemented glucose concentration of media up to 0.5% along with cell growth, but expression level per unit cell mass was inversely proportional to glucose concentration and maximal when glucose was not supplemented at all.

• 조명전기설비학회논문지
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• 제21권6호
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• pp.1-7
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• 2007

본 논문에서는 도로 조명용 광원으로 쓰이는 메탈핼라이드램프, 고압나트륨램프, PLS의 세 가지 광원에 대해 안개 발생시 시인성을 평가하기 위해 특정한 인공 안개 조건하에서 각 광원들에 대한 피시험자들의 평가를 분석하여 명소시에는 고압나트륨램프가 시인성이 좋고 암소시에는 PLS가 시인성이 좋다는 결과를 얻었으며 이 결과는 앞으로의 도로 조명용 광원의 선택에 있어서 도움을 줄 수 있을 것으로 기대된다.

• 대한임상검사과학회지
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• 제38권3호
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• pp.158-165
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• 2006

Although Mycobacterium tuberculosis complex strains remain responsible for the majority of diseases caused by mycobacterial infections worldwide, the increase in HIV infections has allowed for the emergence of other non-tuberculous mycobacteria as clinically significant pathogens. However, Mycobacterium species has a long period of incubation, and requires serious biochemical tests such as niacin, catalase, and nitrate test that are often tedious. The development of rapid and accurate diagnostics can aid in the early diagnosis of disease caused by Mycobacterium. The current DNA amplification and hybridization methods that have been developed target several genes for the detection of mycobacterial species such as hps65, 16S rDNA, rpoB, and dnaj. These methods produce rapid and accurate results. In this study, PCR-restriction fragment length polymorphism analysis(PCR-RFLP) based on the region of the rpoB gene was used to verify the identification of non-tuburculosis Mycobacterium species. A total of 8 mycobacterial reference strains and 13 clinical isolates were digested with restriction enzymes such as Msp I in this study. The results of using this process clearly demonstrated that all 13 specimens were identified by rpoB gene PRA method. The PCR-RFLP method based on the rpoB gene is a simple, rapid, and accurate test for the identification of Mycobacterium.

• Journal of Electrical Engineering and Technology
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• 제9권5호
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• pp.1643-1653
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• 2014

This paper presents the design and implementation of a wind-solar hybrid power system for LED street lighting and an isolated power system. The proposed system consists of photovoltaic modules, a wind generator, a storage system (battery), LED lighting, and the controller, which can manage the power and system operation. This controller has the functions of maximum power point tracking (MPPT) for the wind and solar power, effective charging/discharging for the storage system, LED dimming control for saving energy, and remote data logging for monitoring the performance and maintenance. The proposed system was analyzed in regard to the operation status of the hybrid input power and the battery voltage using a PSIM simulation. In addition, the characteristics of the proposed system's output were analyzed through experimental verification. A prototype was also developed which uses 300[W] of wind power, 200[W] of solar power, 60[W] LED lighting, and a 24[V]/80[Ah] battery. The control system principles and design scheme of the hardware and software are presented.

• 전력전자학회:학술대회논문집
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• 전력전자학회 2015년도 전력전자학술대회 논문집
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• pp.347-348
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• 2015

본 논문은 30kW, 95GHz 자이로트론 구동을 위한 $25kV_{max}$, $10mA_{max}$ 출력 사양의 Body Power Supply (BPS)와 $10V_{max}$, $10A_{max}$ 사양을 가지며 50kV 절연이 요구되는 Heater Power Supply (HPS)의 설계에 관하여 기술한다. 자이로트론 구동 효율 향상에 필요한 BPS는 높은 승압비 달성 및 절연에 대한 신뢰성 확보를 위해 8개의 분리된 코어를 사용하여 변압기를 구성하고, 각 코어간의 보상권선을 이용하는 방식을 제안하여 직렬로 구성된 8개의 고전압 정류부 간의 전압 불균형 문제를 해결 한다. HPS는 50kV 전위가 인가되는 코일 가열을 위해 사용되는 전원으로 내부 고주파 변압기를 이용하여 효율적인 절연 설계를 구현한다. 30kW, 95GHz 자이로트론 구동 시스템의 신뢰성 확보와 전원장치의 높은 전력밀도 달성을 위해 두 전원장치는 공진형 컨버터 토폴로지를 기반으로 설계하고, 시뮬레이션 및 실험을 통해 개발된 전원의 우수성을 검증한다.

• Pediatric Infection and Vaccine
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• 제9권1호
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• pp.95-99
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• 2002

저자들은 내원 1개월 전부터의 고열 복통을 주소로 전원되어 복수를 동반한 EBV 연관성 VAHS로 진단된 1례를 경험하였기에 문헌고찰과 함께 보고하는 바이다.

• Earthquakes and Structures
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• 제16권5호
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• pp.589-599
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• 2019

Conventional buckling restrained braces used in concentrically braced frames are expected to yield in both tension and compression without major degradation of capacity under severe seismic ground motions. One of the weakness points of a standard buckling restrained braced frame is the low post-yield stiffness and thus large residual deformation under moderate to severe ground motions. This phenomenon can be attributed to low post-yield stiffness of core member in a BRB. This paper introduces a multi-core buckling restrained brace. The multi-core term arises from the use of more than one core component with different steel materials, including high-performance steel (HPS-70W) and stainless steel (304L) with high strain hardening properties. Nonlinear dynamic time history analyses were conducted on variety of diagonally braced frames with different heights, in order to compare the seismic performance of regular and multi-core buckling restrained braced frames. The results exhibited that the proposed multi-core buckling restrained braces reduce inter-story and especially residual drift demands in BRBFs. In addition, the results of seismic fragility analysis designated that the probability of exceedance of residual drifts in multi-core buckling restrained braced frames is significantly lower in comparison to standard BRBFs.

• 대한간호학회지
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• 제28권4호
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• pp.1003-1012
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• 1998

The purpose of this research was to identify the effect of human potential seminars on the perceived stigma of adults with epilepsy. The research employed a quasi experimental design and unequivalent control group pre-post design. The test was conducted on 15 adults with epilepsy attending one psychiartric out-patient clinic in Masan City, Korea. The stigma questionnaire was used as a pre-test to these patients. HPS was done ten times for five weeks from May 6, to July 26, 1996. The stigma questionnaire was again given, but this time as a post test. The control group of 14 adults with epilepsy were receiving medication at the same clinic. The human potential seminars were structured by McHolland(1972) and translated by Lee, Hae Seung(1990). The stigma research tool was modified and revised to be appropriate to Korean culture. It consisted of 15 sentences. The internal consistency was 0.92 with Cronbach's α. Research results are as follows. 1) To determine the homogenity of the experimental and control groups, the pre-stigma results were used and democratic-sociologic characteristics, job characteristics and disease related variables were compared. There was no significant difference between the two groups. 2) To identify the relationship between stigma and patient characteristics a pre-test was done. The study used both Mann-Whitney U-test and ANOVA test for statistical analysis. The variables related to stigma were the reason of unemployment and age at onset of epilepsy. 3) The test results of the effect of the human potential seminars on stigma in the patients with epilepsy, showed that stigma in the experimental group was lower than in the control group. The statistical method used to determine the difference between pre and post stigma results was the Wilcoxon signed rank test. The test results were statistically significant at the one percent level. 4) As a follow-up evaluation ten more patients(66.7% of the total) were additionally tested. In order to investigate if the stigmas were different between the pre, post and follow-up, Repeated measure ANOVA was used. The test results showed that the stigma scores were statistically different between the three groups at the one percent level(F=10.06, d.f.=2, p=0.00).

• 생물환경조절학회지
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• 제7권1호
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• pp.35-41
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• 1998

완전제어형 식물공장하에서 인공광원에 따른 결구상추의 생육, 잎끝마름증과 배양액속의 무기이온 변화를 검토하고자 본 실험을 수행하였다. 인공광원에 따른 지상부 생체중과 건물중간의 유의적인 차이는 없었으나 전반적인 생육은 형광등에서 가장 낮게 나타났다. 인공광원중 생육과 램프의 경제성을 고려해 볼때, 고압나트륨등을 사용하는 것이 좋을 것으로 생각된다. 인공광원에 따른 잎끝마름증 발생시기는 정식 후 14일에 처음 발생하였으며, 발생부위는 자엽으로 부터 9∼10번째 잎이었다. 발생원인으로는 빠른 생장율과 상추의 칼륨흡수로 추정되었다. 그리고 인공광원에 따라 배양액속의 무기 성분 변화를 보면, 세처리구 모두 칼슘. 마그네슘, 구리와 아연 함량 등은 축적되는 경향을 보였으며, 전질소, 인, 철과 망간 함량등은 안정적이었다.

• International Journal of Industrial Entomology and Biomaterials
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• 제28권1호
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• pp.10-18
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• 2014

Baculoviruses base vectors come to be regarded as methods for in vivo gene delivery and transient expression to the silkworm. In the case of silkworm, B. mori, two types of baculoviruses, AcNPV (Autographa californica nuclear polyhedrosis virus) and BmNPV (Bombyx mori nuclear polyhedrosis virus), are potentially applicable as vectors. Recently, AcNPV showed promising results with some silkworm strains despite different host-specificities. We searched for a highly-permissive silkworm strain in the B. mori stocks of Kyungpook National University that could produce high levels of recombinant protein. Seventy strains were screened using the recombinant AcNPV/BmA3-Luc virus. Based on the measured luciferase activity, the strains could be divided into three groups, high-, middle-, and low-permissive strains, according to their relative recombinant protein expression levels. At 48 hours post-injection, the luciferase activity in the high-permissive strains was 500-fold greater than that of the low-permissive strains. At 72 hours post-injection, a significant elevation in luciferase activity was observed in the hemocytes of all strains. Then, based on the above results, the High Permissive Strain (HPS) S10 and the Low Permissive Strain (LPS) S39 were pick up and was carried out Dot blotting, RT-PCR and Real time PCR.