• Maxillofacial Plastic and Reconstructive Surgery
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• v.30 no.2
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• pp.205-212
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• 2008

Genetic variation in the human genome occurs on various levels; from the single nucleotide polymorphism to large, microscopically visible chromosome anomalies. It can be present in many forms, including variable number of tandem repeat (VNTRs; e.g., mini- and microsatellites), presence/absence of transposable elements (e.g., Alu elements), single nucleotide polymorphisms, and structural alterations (e.g., copy number variation, segmental duplication, inversion, translocation). Until recently SNPs were thought to be the main source of genetic and phenotypic human variation. However, the use of methods such as array comparative genomic hybridization (array CGH) and fluorescence in situ hybridization (FISH) have revealed the presence of copy number variations(CNVs) ranging from kilobases (kb) to megabases (Mb) in the human genome. There is great interest in the possibility that CNVs playa role in the etiology of common disease such as HIV-1/AIDS, diabetes, autoimmune disease, heart disease and cancer. The discovery of widespread copy number variation in human provides insights into genetic variability among populations and provides a foundation for studies of the contribution of CNVs to evolution and disease.

• Toxicological Research
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• v.34 no.3
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• pp.221-229
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• 2018

Tenofovir nanoparticles are novel therapeutic intervention in human immunodeficiency virus (HIV) infection reaching the virus in their sanctuary sites. However, there has been no systemic toxicity testing of this formulation despite global concerns on the safety of nano drugs. Therefore, this study was designed to investigate the toxicity of Tenofovir nanoparticle (NTDF) on the liver and kidney using an animal model. Fifteen adult male Sprague-Dawley (SD) rats maintained at the animal house of the biomedical resources unit of the University of KwaZulu-Natal were weighed and divided into three groups. Control animals (A) were administered with normal saline (NS). The therapeutic doses of Tenofovir (TDF) and nanoparticles of Tenofovir (NTDF) were administered to group B and C and observed for signs of stress for four weeks after which animals were weighed and sacrificed. Liver and kidney were removed and fixed in formal saline, processed and stained using H/E, PAS and MT stains for light microscopy. Serum was obtained for renal function test (RFT) and liver function test (LFT). Cellular measurements and capturing were done using ImageJ and Leica software 2.0. Data were analysed using graph pad 6, p values < 0.05 were significant. We observed no signs of behavioural toxicity and no mortality during this study, however, in the kidneys, we reported mild morphological perturbations widening of Bowman's space, and vacuolations in glomerulus and tubules of TDF and NTDF animals. Also, there was a significant elevation of glycogen deposition in NTDF and TDF animals when compared with control. In the liver, there were mild histological changes with widening of sinusoidal spaces, vacuolations in hepatocytes and elevation of glycogen deposition in TDF and NTDF administered animals. In addition to this, there were no significant differences in stereological measurements and cell count, LFT, RFT, weight changes and organo-somatic index between treatment groups and control. In conclusion, NTDF and TDF in therapeutic doses can lead to mild hepatic and renal histological damage. Further studies are needed to understand the precise genetic mechanism.

• Parasites, Hosts and Diseases
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• v.50 no.3
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• pp.199-205
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• 2012

Toxoplasmic encephalitis is caused by reactivation of bradyzoites to rapidly dividing tachyzoites of the apicomplexan parasite Toxoplasma gondii in immunocompromised hosts. Diagnosis of this life-threatening disease is problematic, because it is difficult to discriminate between these 2 stages. Toxoplasma PCR assays using gDNA as a template have been unable to discriminate between an increase or decrease in SAG1 and BAG1 expression between the active tachyzoite stage and the latent bradyzoite stage. In the present study, real-time RT-PCR assay was used to detect the expression of bradyzoite (BAG1)- and tachyzoite-specific genes (SAG1) during bradyzoite/tachyzoite stage conversion in mice infected with T. gondii Tehran strain after dexamethasone sodium phosphate (DXM) administration. The conversion reaction was observed in the lungs and brain tissues of experimental mice, indicated by SAG1 expression at day 6 after DXM administration, and continued until day 14. Bradyzoites were also detected in both organs throughout the study; however, it decreased at day 14 significantly. It is suggested that during the reactivation period, bradyzoites not only escape from the cysts and reinvade neighboring cells as tachyzoites, but also converted to new bradyzoites. In summary, the real-time RT-PCR assay provided a reliable, fast, and quantitative way of detecting T. gondii reactivation in an animal model. Thus, this method may be useful for diagnosing stage conversion in clinical specimens of immunocompromised patients (HIV or transplant patients) for early identification of tachyzoite-bradyzoite stage conversion.

• Journal of Korean society of Dental Hygiene
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• v.10 no.6
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• pp.1107-1119
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• 2010

Objectivs : The purpose of this study is to provide basic data for the use of oral hygiene devices and to improve oral health care continuously by encouraging the use of oral hygiene devices. Methods : The survey was carried out by questionnaire research targeting 395 respondents in metropolitan area in June-August 2009. The collected data is analyzed by statistics program SPSS version 12.0. 1. General characteristics of subjects are analyzed by percentage and descriptive statistics. 2. Recognition about how to use oral hygiene devices, usage of oral hygiene devices, recognition of dental brush usage, Effect after using oral hygiene devices are analyzed by mean difference test(t, F verification). 3. Effect after using oral hygiene devices, relation of oral hygiene devices usage with subjective oral health status, oral hygiene devices usage and participation in education are analyzed by Correlation, Regression analysis. Results : 1. Investigation shows that most oral health devices are rarely used except toothbrush(4.57) in case of the use of oral hygiene devices. 2. In the case of awareness of oral hygiene devices usage, almost everyone knows how to use dental brushes(MEAN 4.16) the most. And the next ones are like these-tooth picks(MEAN 3.38), dental floss(MEAN 3.28), mouth rinse(MEAN 3.24). And they don't know how to use the other oral hygiene devices usage. 3. respondents who use dental floss(${\beta}$=.238, p<.001), interdental brush(${\beta}$=.242, p<.001) and water pik(${\beta}$ =.180, p<.05) recognize that the result and effect after using them are more positive than the others in different case. 4. Respondents who use toothbrush(${\beta}$=.119), dental floss(${\beta}$=.120) and interdental brush(${\beta}$=.136). frequently evaluate their subjective oral health status positively in case of frequency of using oral hygiene devices and subjective oral health status(p<.05). 5. Respondents who use toothbrush and interdental brush properly and frequently, who are older than the others show more concern about participating in education of "How to use oral hygiene devices" than the others(p<.05). Conclusions : In conclusion, to prevent oral disease, the opportunity of participating in education about correct knowledge and proper way should be expanded. And we should contribute to oral health improvement through developing, executing and informing systematic, popular oral health education with active one.

• Korean Journal of Health Education and Promotion
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• v.11 no.1
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• pp.43-56
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• 1994

Although the officially confirmed incidence of the acquired immune deficiency syndrome(AIDS) has remained low thus far in Korea, the progression of the pandemic has been rapid, concerned about an eventual increase in transmission. Until a vaccine or cure is found, public information and education remain an important weapon against AIDS. For the year 2000, the current WHO projection is that there will be a cumulative total of 40 million HIV infections in man, woman and children, of which more than 90％ will be in the developing countries. The incerased mortality among adults and children has raised concerns that AIDS may become so devastaing as to reverse positive population growth rates and wipe out entire population. In order to plan for the rising incidence of patient with AIDS, an assessment of AIDS-related cognition and behavior, attitude of the recognized high-risk groups is necessary. Considering that undergraduates have sexuality active and more oriented toward sexual concerns than other ages. Adolescents today are more sexually involved than in the past: The media, including films, music, and television, reinforce sexual concerns by preseating images of highly sexual adolescents. Incidence of both petting and sexual intercourse in higher and occurs at earlier ages, they are important reservoir of AIDS infection. Hence, it has become important to emymerats and describe the knowledge, attitude and behavior of them, I want.. This information will eventually guide the direction of change in public policies and education programs to meet the challenges of AIDS. The objective of this study were to identity Knowledge, Attitude, Experience of Sex and AIDS in Korea. The data were collected by survey at Korean Federation for AIDS Affairs, INC. and J university, K junior college. After October in 1993, surveyed with 507 student during their's college days in Seoul. The questionnaire was designed to provide information on sex and AIDS-related knowledge and attitudes and experience. AIDS-related knowledge was measured by using 26 questions on mode of transmission and AIDS-related attitudes were measured by 7 questions, AIDS-related experience was measured by 5 questions. There were the main dependent variables in our study, In conclusion, students during their university day need to develop AIDS-preventive education programs. Clearly, the many AIDS-prevention educational effort need to be guided by well trained health agents. Financial supports from all sectors (i.e., public and private) of our society are required to achieve that end. Further researches on AIDS-related Knowledge and Attitude and behaviors of undergraduates are needed for the development of comprehensive AIDS prevention programs.

• Tuberculosis and Respiratory Diseases
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• v.48 no.3
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• pp.377-382
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• 2000

Idiopathic CD4+ T-lymphocytopenia is defined as a depletion of CD4+ lymphocytes below $300/mm^3$ in the absence of HIV infection or other known causes of immunodeficiency. Many infectious diseases have been reported to be associated with idiopathic CD4+ T-lymphocytopenia, and there have also been a few cases of mycobacterial infection in idiopathic CD4+ T-lymphocytopenia. Until now, it has been unclear as to whether CD4+ T-lymphocytopenia is a predisposing factor for or a consequence of the mycobacterial infection. Pulmonary alveolar proteinosis is an uncommon disease characterized by the intraalveolar deposition of amorphous granular material that stains positive with PAS, and its association with mycobacterial infection has rarely been reported. Recently, we experienced a previously healthy young man who had been diagnosed as idiopathic CD4+ T-lymphocytopenia with disseminated mycobacterium kansasii infection and pulmonary alveolar proteinosis, and report this case.

• Journal of Life Science
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• v.26 no.10
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• pp.1196-1201
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• 2016

The ovary parts of Nelumbo nucifera were extracted in 80% methanol (MeOH), and the concentrated extract was then partitioned using n-hexane, ethyl acetate (EtOAc), n-butanol (n-BuOH), and H₂O, successively. Using an octadecyl silica gel (ODS) column, silica gel (SiO₂) column chromatography, and a HPLC purification system, five compounds were isolated from the n-hexane fraction obtained from the extract of N. nucifera ovary. The chemical structures of the metabolites were determined using several spectroscopic methods, including NMR and GC/MS and MS of 1-eicosanol (1), cycloartenol (2), trans-squalene (3), pentadecanoic acid (4), and β-sitosterol (5). This study is a first attempt to isolate and identify secondary metabolites from the ovary of N. nucifera. The results indicated that the extract of N. nucifera ovary has biological effects, such as antibacterial and -tumor activity. Therefore, it could decrease the risk of HIV transmission through breastfeeding.

• Journal of Microbiology and Biotechnology
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• v.28 no.6
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• pp.849-859
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• 2018

Herpes simplex virus type 2 (HSV-2) infection has been a public health concern worldwide. It is the leading cause of genital herpes and a contributing factor to cervical cancer and human immunodeficiency virus (HIV) infection. No vaccine is available yet for the treatment of HSV-2 infection, and routinely used synthetic nucleoside analogs have led to the emergence of drug resistance. The small molecule $Retro-2^{cycl}$ has been reported to be active against several pathogens by acting on intracellular vesicle transport, which also participates in the HSV-2 lifecycle. Here, we showed that Retro-2.1, which is an optimized, more potent derivative of $Retro-2^{cycl}$, could inhibit HSV-2 infection, with 50% inhibitory concentrations of $5.58{\mu}M$ and $6.35{\mu}M$ in cytopathic effect inhibition and plaque reduction assays, respectively. The cytotoxicity of Retro-2.1 was relatively low, with a 50% cytotoxicity concentration of $116.5{\mu}M$. We also preliminarily identified that Retro-2.1 exerted the antiviral effect against HSV-2 by a dual mechanism of action on virus entry and late stages of infection. Therefore, our study for the first time demonstrated Retro-2.1 as an effective antiviral agent against HSV-2 in vitro with targets distinct from those of nucleoside analogs.

• Proceedings of The Korean Society of Health Promotion Conference
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• 2005.09a
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• pp.121-149
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• 2005

미국 캘리포니아 주의 건강문제로는 흡연, HIV/AIDS, 천식, 정신질환, 아동비만, 유방암, API, 건강한 생활양식 형성, 인종간의 건강격차 등을 들 수 있다. 이러한 건강문제를 해결하기 위해 캘리포니아주에서 진행되는 지역사회 중재전략은 지역사회 단체와의 협력이라는 특성을 지니고 있으며, 도시지역의 노인이나 다양한 인종들을 대상으로 하는 프로그램들의 경우는 교회를 통한 접근방법이 빈번하게 활용된다. 중재 프로그램들은 지역사회 조직모형과 역량강화의 개념을 활용하며, 문화적으로 다른 배경을 가진 인구집단을 대상으로는 이들의 요구에 맞는 프로그램을 진행하는 형태로 기존의 프로그램을 채택하는 것이 아닌 이들의 다양한 요구에 맞도록 수정된 프로그램을 제공하고 있다. 개인 간 의사소통을 활용하여 사회적 관계망의 활성화를 도모하며, 일반인이 건강지도자가 되는 교육모형도 활용하고 있다. 한국교민사회 자문위원회(Korean Community Advisory Board, KCAB)는 CDCPRC(CFCH)의 기금으로 진행되는 연구 프로젝트를 수행할 목적으로 1993년 설립되었다. 장기적인 지역사회 건강증진을 위하여 전문가와 일반인 지도자들이 지역사회에서 협력하는 방법을 활용하며 요구도 진단, 수행 평가, 건강옹호와 같은 다양한 사업을 진행한다. 회원들은 핵심 회원과 일반회원으로 구분되고 이들은 사업의 수행과 관련된 의사결정에 도움을 주게 된다. 한국교민사회 자문위원회가 수행한 프로그램은 'Health is Strength', 'Virtual Village Program'이 있으며, 현재는 'Quitting is Winning'이라는 미국거주 한국 남성을 대상으로 하는 인터넷 금연 프로그램을 수행하고 있다. 또한 캘리포니아 주 거주 한국인의 건강상태에 대한 조사를 진행하고 그 결과를 보고하는 프로젝트를 진행하고 있다. 이들이 하는 주요 업무는 지역에서 발생되는 건강문제에 대한 우선순위를 파악할 수 있도록 하여 한국교민들의 건강요구를 파악하고 사업의 우선순위를 설정하는데 도움을 주고 있다. 센터에서 진행되는 한국교민 대상 연구나 사업에 가장 효과적일 수 있는 방법에 대한 지침이나 정보를 제공한다. 또한 문화적으로 민감한 사항이나 적절성 여부에 대한 확인을 하며 연구결과를 보고하고 확산하는데 도움을 주고 있다. 캘리포니아 주는 다양한 인종들이 거주하는 지역이다. 따라서 문화적 민감성이나 문화적 대응전략, 문화적 적절성은 건강증진사업을 수행할 경우에 매우 중요한 이슈가 된다. 또한 이들은 소수민족인 경우가 많아 언어장벽으로 인하여 의료서비스 이용에 제한을 받게 된다. 따라서 보건의료전문가들은 이러한 문화적 요소들을 능숙하게 다루는 기술이 필요한데, 이러한 기술이란 문화가 다른 사람들 간에는 갈등이 존재한다는 점을 인식하고, 건강관련 믿음과 건강행동에 영향을 미치는 사회문화적 요인들을 파악하고, 대안적 해결책을 제시하며, 결과를 평가할 수 있는 능력을 의미한다. 이러한 영역에서 health care interpreter는 소수인종의 보건의료 서비스의 원활한 이용을 위하여 매우 활발한 역할을 수행하고 있다.

• Tuberculosis and Respiratory Diseases
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• v.43 no.6
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• pp.862-870
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• 1996

Background : Multidrug-resistant tuberculosis(MDR-Tb) has been increased not only in Asia but also in Western society, which may cause public health problems and reduce the efficacy of treatment of tuberculosis. In Western society HIV infection is believed to do a central role in increasing incidence of MDR tuberculosis, but MDR-Tb in Korea may be somewhat different about clinical features, underlying disorders, and prognosis. Goble et al reponed that overall treatment failure rate in MDR-Tb including resistance to isoniazid(INH) and rifampin (RFP) was 44 %. The aim of this study is to find the treatment result in Korea and the factors determining the prognosis. Methods: A retrospective study of pulmonary tuberculosis cultured M. tuberculosis from sputum or bronchial washing fluid between 1986 through 1992 was conducted in the Seoul Paik Hospital, Inje University. We reviewed clinical courses of 141 patients, who had a tuberculosis with resistance to 2 or more drugs including isoniazid(INH) and rifampin(RFP). One hundred and 4 patients of 141 patients had completed treatment and followed up for more than one year. Results: Of 104 (mean age $43.6{\pm}16.7$, M: F=63 : 41) patients with sufficient follow-up data, 73(84.6%) patients responded which is defined as negative Sputum cultures for at least 3 consecutive months. Seven patients(6.7%) had a failure in negative conversion and 9(8.7%) of the patients who initially responded relapsed. Overall treatment failure rate was 15.4%, Patients who were treated for less than 12 months had a higher relapse rate(12.3%) than 18 months(4.9%). And there was a statistically significant correlation between the relapse rate and the number of drugs to which isolates wera resistant(p<0.05). Conclusion : The treatment failure rate of MDR-Tb in Korea was lower than previous studies in western Country and the major determining factor of prognosis was the number of resistant drugs to M. tuberculosis at drug sensitivity test. For reducing the relapse rate, we recommend more than 12 months of treatment for MDR tuberculosis.