Browse > Article
http://dx.doi.org/10.5352/JLS.2016.26.10.1196

Isolation and Identification of Secondary Metabolites from the Ovary of Nelumbo nucifera  

Ji, Seung-Heon (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Lee, Jae-Won (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Lee, Seung-Eun (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Lee, Young-Seob (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Kim, Geum-Soog (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Ahn, Young-Sup (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Baek, Nam-In (Department of Oriental Medicinal Materials & Processing, Kyung Hee University)
Lee, Yi (Department of Industrial Plant Science and Technology, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
Lim, Heung-Bin (Department of Industrial Plant Science and Technology, College of Agriculture, Life and Environment Sciences, Chungbuk National University)
Lee, Dae Young (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
Publication Information
Journal of Life Science / v.26, no.10, 2016 , pp. 1196-1201 More about this Journal
Abstract
The ovary parts of Nelumbo nucifera were extracted in 80% methanol (MeOH), and the concentrated extract was then partitioned using n-hexane, ethyl acetate (EtOAc), n-butanol (n-BuOH), and H2O, successively. Using an octadecyl silica gel (ODS) column, silica gel (SiO2) column chromatography, and a HPLC purification system, five compounds were isolated from the n-hexane fraction obtained from the extract of N. nucifera ovary. The chemical structures of the metabolites were determined using several spectroscopic methods, including NMR and GC/MS and MS of 1-eicosanol (1), cycloartenol (2), trans-squalene (3), pentadecanoic acid (4), and β-sitosterol (5). This study is a first attempt to isolate and identify secondary metabolites from the ovary of N. nucifera. The results indicated that the extract of N. nucifera ovary has biological effects, such as antibacterial and -tumor activity. Therefore, it could decrease the risk of HIV transmission through breastfeeding.
Keywords
Column chromatography; GC/MS; Nelumbo nucifera; NMR;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Ragara, C. Y., Tiu F. and Rideout, J. A. 2004. New cycloartenol esters from ixora coccinea. Nat. Prod. Res. 18, 319-323.   DOI
2 Sin, M. K., Kim, C. M., Ahn, D. K. and Lee, K. S. Dictionary of Chinese Materia Mediaca (in Korean), Jungdam Publishing: Seoul, Korea, 1997, p 2958.
3 Smith, T. J. 2000. Squalene: potential chemopreventive agent. Expert Opin. Investig. Drugs 9, 1841-1848.   DOI
4 Sohn, D. H., Kim, Y. C., Oh, S. H., Park, E. J., Li, X. and Lee, B. H. 2003. Hepatoprotective and free radical scavenging effects of Nelumbo nucifera. Phytomedicine 10, 165-169.   DOI
5 Villamor, E., Koulinska, I. N., Furtado, J., Baylin, A., Aboud, S., Manji, K., Campos, H. and Fawzi, W. 2007. Long-chain n-6 polyunsaturated fatty acids in breast milk decrease the risk of HIV transmission through breastfeeding. Am. J. Clin. Nutr. 86, 682-689.
6 Wu, L., Chen, Z. L., Su, Y., Wang, Q. H. and Kuang, H. X. 2015. Cycloartenol triterpenoid saponins from Cimicifuga simplex (Ranunculaceae) and their biological effects. Chin. J. Nat. Med. 13, 81-89.
7 Assmann, G., Cullen, P., Erbey, J., Ramey, D. R., Kannenberg, F. and Schulte, H. 2006. Nutr. Metab. Cardiovas. Dis. 16, 13-21.   DOI
8 Jung, H. A., Jung, Y. J., Hyun, S. K., Min, B. S., Kim, D. W., Jung, J. H. and Choi, J. S. 2010. Selective cholinesterase inhibitory activities of a new monoterpene diglycoside and other constituents from nelumbo nucifera stamens. Biol. Pharm. Bull. 33, 267-272.   DOI
9 Chang, M. S., Kim, H. Mi., Yang, W. M., Kim, D. R., Park, E. H., Ko, E. B., Choi, M. J., Kim, H. Y., Oh, J. H., Shim, K. J., Yoon, J. W. and Park, S. K. 2007. Inhibitory effects of nelumbo nucifera on tyrosinase activity and melanogenesis in clone M-3 melanocyte cells. Kor. J. Herbology 22, 87-94.
10 Chena, S., Fanga, L., Xic, H., Guanc, L., Fangd, J., Liua, Y., Wuc, B. and Li, S. 2012. Simultaneous qualitative assessment and quantitative analysis of flavonoids in various tissues of lotus (Nelumbo nucifera) using high performance liquid chromatography coupled with triple quad mass spectrometry. Anal. Chim. Acta. 724, 127-135.   DOI
11 Kamboj, A. and Saluju, A. K. 2011. Isolation of stigmasterol and β-sitosterol from petroleum ether extract of aerial parts of Ageratum conyzoides (asteraceae). Int. J. Pharm. Pharm. Sci. 3, 975-1491.
12 Lee, K. S., Kim, M. G. and Lee, K. Y. 2006. Antioxidative activity of ethanol extract from lotus (Nelumbo nucifera) leaf. J. Kor. Soc. Food Sci. Nutr. 35, 182-186.   DOI
13 Kashiwada, Y., Aoshima, A., Ikeshiro, Y., Chen, Y. P., Furukawa, H., Itoigawa, M., Fujioka, T., Mihashi., Cosentino, L. M., Morris-Natschkeg, S. L. and Lee, K. H. 2005. Anti-HIV benzylisoquinoline alkaloids and flavonoids from the leaves of Nelumbo nucifera, and structure–activity correlations with related alkaloids. Bioorgan. Med. Chem. 13, 443-448.   DOI
14 Kim, K. H., Chang, S. W., Ryu, S. Y., Choi, S. U. and Lee, K. R. 2009. Phytochemical constituents of Nelumbo nucifera". Nat. Prod. Sci. 15, 90-95.
15 Kim, M. S., Park, Y. J. and Sohn, Y. W. 2010. Antioxidative effect and melanogenesis of Nelumbo nucifera stamen extract on cultured human skin melanoma cells injured by hydrogen peroxide. Kor. J. Plant Res. 23, 145-150.
16 Kubo, I., Muroi, H. and Kubo A. 1993. Antibacterial activity of long-chain alcohols against Streptococcus mutans. J. Agric. Food Chem. 41, 2447-2450.   DOI
17 Kutitomo, J., Yoshikawas, Y., Takaka, S. Y., Imori, Y. and Isor, K. 1973. Alkaloids of Nelumbo nucifera. Phytochemistry 12, 699-701.   DOI
18 Mazumder, U. K., Malaya, G., Pramanik, G., Mujhopadhyay, R. K. and Swarnari, S. 1992. Antifertility activity of seed of Nelumbo nucifera in mice. Ind. J. Exp. Biol. 13, 533-534.
19 Mukherjee, P. K., Mukherjee, D., Maji, A. K., Rai, S. and Heinrich, M. 2009. The sacred lotus (Nelumbo nucifera) - phytochemical and therapeutic profile. J. Pharm. Pharmacol. 61, 407-422.   DOI
20 Moon, S. M., Kim, H. J. and Ham, K. S. 2003. Purification and characterization of polyphnol oxidase from lotus root(Nelumbo nucifera G.). J. Food Sci. Technol. Col. 35, 791-796.
21 Park, C. H., Hur, J. M., Song, K. S. and Park, J. C. 2007. Phenolic Compounds from the leaves of Nelumbo nucifera showing DPPH radical scavenging effect. Kor. J. Pharmacogn. 38, 263-269.