• Journal of the Korean Chemical Society
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• v.31 no.5
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• pp.452-456
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• 1987

Acidic strength of benzenesulfonic acid (HBs) and it's derivatives, p-toluenesulfonic acid(HTs), p-chlorobenzenesulfonic acid(HCs) and m-nitrobenzenesulfonic acid(HNs), were measured in the anhydrous acetic acid medium by spectrophotometry. p-naphtholbenzein (PNB) was used as an indicator base and the ionization constants of HTs, HBs, HCs and HNs were $3.5{\times}10^2,\;4.1{\times}10^2,\;19.3{\times}10^2\;and\;50{\times}10^2$, respectively, at 20.0${\pm}$0.1$^{\circ}$C.

• Journal of Microbiology and Biotechnology
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• v.26 no.2
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• pp.213-225
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• 2016

To reduce attrition in drug development, it is crucial to consider the development and implementation of translational phenotypic assays as well as decipher diverse molecular mechanisms of action for new molecular entities. High-throughput fluorescence and confocal microscopes with advanced analysis software have simplified the simultaneous identification and quantification of various cellular processes through what is now referred to as high-content screening (HCS). HCS permits automated identification of modifiers of accessible and biologically relevant targets and can thus be used to detect gene interactions or identify toxic pathways of drug candidates to improve drug discovery and development processes. In this review, we summarize several HCS-compatible, biochemical, and molecular biology-driven assays, including immunohistochemistry, RNAi, reporter gene assay, CRISPR-Cas9 system, and protein-protein interactions to assess a variety of cellular processes, including proliferation, morphological changes, protein expression, localization, post-translational modifications, and protein-protein interactions. These cell-based assay methods can be applied to not only 2D cell culture but also 3D cell culture systems in a high-throughput manner.

• Journal of the Korean housing association
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• v.19 no.5
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• pp.93-102
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• 2008

In Korea, a movement for forming intentional communities is gathering people's interests to improve individualistic living environment, and to create a humanistic lifestyle. However, it is difficult to say if its management is successful or not, because intentional community is just in the experimental stage in Korea. The purpose of this study is to identify actual condition of residents' participation in forming process, shared activities in their daily lives, residents' regulation, common facilities and its management in order to offer basic information for revitalization of intentional communities in Korea. 7 intentional communities including eco-friendly villages, religious communities and a cooperative housing community were collected as the study objects. Upon analysis, those communities were divided into two groups according to their purpose of establishment; "HC (Housing-life focused Community includes cooperative housing community and eco-friendly community)" and "IC (Ideology focused Community includes ideology community and religious community)" in order to identify difference in residents' participation between the two groups. In-dept interviews with representatives of 7 intentional communities by a structured questionnaire were used as study method. The findings of this study are as follows; In general, more active residents' participation is identified in ICs than in HCs. There is no common house, which is considered as essential in intentional community, in HCs, while it was facilitated in all ICs. Role of leader seems more important in ICs than in HCs. About the ownership of housing and land, private owned is common in HCs, while community owned is common in ICs. Shared activities and residents' regulations are evidently less in most HCs than in ICs. Furthermore, in order to run a community sustainable, it is crucial to encourage sense of community among residents, and developing common house and activity programs. Common house design, which can promote proactive residents' participation in shared activity should be studied fitted to Korean circumstances. Above all, proactive participation in the shared activities is one of the most important factors in intentional community.

• Clinical and Experimental Reproductive Medicine
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• v.25 no.1
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• pp.9-16
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• 1998

This study was performed to investigate the stimulating effect on oocyte maturation and cumulus cell expansion in TC199 media by human cord serum (HCS) supplementation. Immature mouse oocyte cumulus complexes (OCCs) were cultured in TC199 media supplemented with bovine serum albumin (BSA), HCS and human chorionic gonadotropin (hCG) instead of luteinizing hormone (LH) respectively, and the expression of cumulus expansion and oocyte maturation were observed. After 4hr and 24hr culture with or without OCCs, media containing 0.4% BSA, 10% HCS and 10 IV hCG respectively were collected and analyzed for changing concentrations of estradiol $(E_2)$, progesterone $(P_4)$, testosterone (T), and $PGF_{2\alpha}$. There were no elevation of $E_2$, T, and $PGF_{2\alpha}$ by OCCs culture, but minute elevation of $P_4$ level by 24hr OCCs culture in hCG supplementation (p=0.048). The stimulating pattern of cumulus expansion of OCCs by HCS and hCG supplementation was similar to our previously report using Ham's F-10 media, however oocyte maturation rates after 24hr OCCs culture in all media were increased by $20\sim30%$ compared to Ham's F-10 media. These results suggest that LH in HCS induce cumulus expansion probably by $P_4$ secretion of OCCs, and TC199 is efficient media for immature mouse oocyte maturation.

• Journal of the Korean Recycled Construction Resources Institute
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• v.10 no.4
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• pp.395-401
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• 2022

In this study, the transfer length of strand was measured for three types of HCS member(H200, H320, and H400) manufactured by the pretension method. Strain gauges were attached in longitudinal direction at regular intervals on the sides of the HCS members, and the strain was measured during the cutting process of HCS. The stain at the cutting point was zero, and gradually increases in the central direction of the member, converging to a constant value after passing the transfer length. In the case of H200 members in which the strands were arranged one by one, the transfer lengths were formed within the range of the design equation (up to 762 mm). The transfer length of the H320 member and the H400 member, in which three strands were arranged, was higher than the design range (850 mm or more).

• Journal of Korea Society of Digital Industry and Information Management
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• v.5 no.2
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• pp.1-11
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• 2009

This study presents HCWS algorithm for researcher grouping on a weighted researcher network. The weights represent intensity of connections among researchers based on the number of co-authors and the number of co-authored research papers. To confirm the validity of the proposed technique, this study conducted an experimentation on about 80 research papers. As a consequence, it is proved that HCWS algorithm is able to bring about more realistic clustering compared with HCS algorithm which presents semantic relations among researchers in simple connections. In addition, it is found that HCWS algorithm can address the problems of existing HCS algorithm; researchers are disconnected since their connections are classified as weak even though they are strong, and vise versa. The technique described in this research paper can be applied to efficiently establish social networks of researchers considering relations such as collaboration histories among researchers or to create communities of researchers.

• Proceedings of the Korea Concrete Institute Conference
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• 2004.05a
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• pp.56-59
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• 2004

This paper presents the consideration of design guideline for underground HCS system, composite spancrete slab, under axial and bending force. Serviceability design requirements for continuous composite spancrete slab subjected axial force, which are allowable stress and deflection, are compared. Flexural strengths are evaluated by design guideline using strain-compatibility method. The results showed that stresses of spancrete and topping concrete, especially at the ends of beam, have much effect on design loads. Maximum service loads for tested specimens are proposed by allowable stress.

• 한국연소학회:학술대회논문집
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• 2004.06a
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• pp.173-179
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• 2004

Combustion of refuse derived fuel(RDF) and refuse plastic fuel (RPF) was carried out in a lab-scale circulating fluidized bed. Experiment was investigated cold flow visualization. RDF was made by C & tech and RPF was made by KRS. The results include distribution of temperature in the combustion chamber, and concentrations of flue gas such as $O_2$, $CO_2$, CO, $NO_x$ and HCs Micro G.C(gas chromatograph) was employed to find out concentration of He Temperature distribution was different when RDF and RPF were burnt respectably. As air ratio became increased, $CO_2$, CO, and total of HCs emissions were decreased. According to the number of carbon atom of HCs, HC were classified as five kinds of HC.

• Proceedings of the KIEE Conference
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• 2015.07a
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• pp.1415-1416
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• 2015

본 논문은 외산 HCS장비에서 획득한 세포 이미지를 기반으로 다기능 분석 소프트웨어 개발을 위한 기초 연구로서 세포의 외곽선 검출 알고리즘을 제안한다. 기존 체인코드 알고리즘의 탐색 횟수를 줄여 ROI 검출 속도 및 전체 시스템의 성능을 높이고자 하였다. 기존 알고리즘과 제안한 알고리즘을 C언어로 구현하여 실제 영상에 실험해 본 결과 정확한 세포의 ROI 검출뿐만 아니라 속도 향상의 효과를 확인하였다.

• Journal of Embryo Transfer
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• v.6 no.1
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• pp.33-40
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• 1991

In vitro developmental ability of early preimplantation monse embryos was shown to be depend on the embryonic stages, media and snpplements and their interaction(Experiment 1). The development of I-cell embryos were more promoted in the complex medinm(Ham's Fl0) than in the simple one(m-KRB), but that of 2-cell embryos showed the reverse effect. The bovine serum albumin(BSA) as a medium snpplement more promoted the development of I- and 2-cell embryos, compared with human fetal cord serum(HCS). On the other hand, the harmful effect of HCS was especially shown on the early cleavage in the embryonic development of the two stages. The effect of serum, in the respect of interaction between media and snpplements. was also more significantly appeared in m-KRB than Ham's Fl0. In the experiment 2, when the harmful effect of HCS was compared with that of fetal bovine serum(FBS), the former more promoted the development of l - and 2-cell embryos than the latter. The effect of HCS was more significantly shown in the development of I-cell than that of 2-cell embryos. Conclusively, as I- and 2-cell embryos were different in the requirements for the in vitro development. the optimal medium and supplement have to be selected for each embryonic stage. It is also respected to the better result if it take into consideration into the kinds of sera when serum is used for culture of early preimplantation embryos.