• Journal of Korean Medicine for Obesity Research
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• v.23 no.2
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• pp.69-77
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• 2023

Objectives: Atriplex gmelinii C. A. Meyer is a halophyte belonging to the Chenopodiaceae family, and its young leaves and stems are used as fodder for livestock. The aim of the present study was to investigate the effects of A. gmelinii extract and its solvent fractions on lipid accumulation during adipogenesis of 3T3-L1 preadipocytes. Methods: The samples of A. gmelinii were separately extracted using methylene chloride and methanol. Subsequently, they were combined to formulate the initial extract, which was then partitioned based on polarity to prepare solvent fractions. Oil Red O staining was employed to measure lipid accumulation during the differentiation of 3T3-L1 preadipocytes. To verify cytotoxicity in 3T3-L1 cells, MTT assays were conducted. The expression levels of transcription factors in 3T3-L1 preadipocytes were measured through Western blotting analysis. Results: At 50 ㎍/mL, treatment of A. gmelinii extract and its solvent fractions during the differentiation of 3T3-L1 preadipocytes significantly diminished lipid accumulation with no noteworthy cytotoxicity on cell viability. Additionally, when investigating the biochemical pathways that underlie the prevention of lipid accumulation using solvent fractions, it was found that the n-BuOH and n-hexane fractions significantly decreased the expression of key transcription factors involved in the generation of fat, such as peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer binding protein α (C/EBPα), and sterol regulatory element-binding protein-1c (SREBP1c). Conclusions: These findings indicate that A. gmelinii can effectively reduce the accumulation of fat in 3T3-L1 adipocytes, making it a potentially valuable material for mitigating and preventing obesity.

• Journal of Marine Bioscience and Biotechnology
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• v.16 no.1
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• pp.1-18
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• 2024

Coastal dunes must be conserved. Their native halophytes support coastal geography while their symbiotic microorganisms help vegetation thrive. The Goraebul coast has the largest, well-conserved dune system on the East Sea of the Korean Peninsula due to a climax mixed halophyte (C. soldanella, C. kobomugi, and E. mollis) vegetation support. This study identified rhizobacteria and their diversity in mixed halophyte communities unique to Goraebul. Five phyla, 12 genera, and 21 species were identified based on 16S rDNA sequences from 65 isolates. The phylum Bacillota, class Bacillota, order Bacillales, and family Bacillaceae were identified, with Bacillus as the dominant genus (46.15%). The richness and Shannon's diversity were higher at the species than at the genus level due to the dominance of Bacillus; however, various Bacillus species (7) were identified. Therefore, the climax mixed vegetation adapted to the Goraebul coast may exert natural selection pressure in favor of the common characteristics of Bacillus. However, despite this advantage, the Shannon equitability (0.86), Simpson (0.08), and Shannon diversity (2.79) indexes indicate a stable rhizosphere cluster and the climax mixed vegetation is affected by symbiotic relationships between healthy rhizosphere microbiota.

• Korean Journal of Veterinary Research
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• v.46 no.4
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• pp.323-326
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• 2006

Suaeda (S.) asparagoides $M_{IQ}$, one of the halophyte groups, has been used as a folk remedy for digestive disturbances in Korea. However, its pharmacological activity on gastrointestinal motility has not been reported yet. In this study, the effects of this halophyte extracts with various solvent fractions (ethanol, hexane, chloroform, ethyl acetate, butanol, and water) on mice ileal spontaneous motility was examined. All solvent fractions at the concentration of $100{\mu}g/ml$ showed inhibitory actions on spontaneous motility of ileum with the potency order of water > 70% ethanol > hexane ${\gg}$ chloroform ${\geq}$ butanol ${\geq}$ ethyl acetate, respectively. In addition, the water fraction of extracts from S. asparagoides $M_{IQ}$ (WFSA) dose-dependently ($1-100{\mu}g/ml$) inhibited the amplitude of spontaneous phasic contraction and area under the contractile curve (AUC). The inhibitory effect of water fraction at the concentration of $10{\mu}g/ml$ was not affected by tetrodotoxin (TTX), $Na^+$ channel blocker ($1{\mu}M$), and $N^w$-nitro-L-arginine Methyl Ester (L-NAME), nitric oxide synthase inhibitor ($100{\mu}M$). However, cyclopiazonic acid (CPA, $10{\mu}M$), inhibitor of sarcoplasmic reticulum $Ca^{2+}$-ATPase, almost blocked the inhibitory effects of WFSA ($10{\mu}g/ml$) on the spontaneous phasic contraction of mouse ileum. But, CPA did not inhibit the lowering basal tone effects of WFSA. The result of this study showed that various extracts of S. asparagoides $M_{IQ}$ induce inhibitory effects on spontaneous contraction of mice ileal segments. More over, the polar solvent fractions were shown to be more potent than non-polar solvent fractions. The effects of S. asparagoides $M_{IQ}$ extracts are not mediated by nerve or nitric oxide. The inhibitory effects of WFSA at least partially mediated by sarcoplasmic reticulum $Ca^{2+}$-ATPase. However, further study is required to determine the exact pharmacological mechanisms of this halophyte on its gastrointestinal motility inhibitory effects.

• Journal of Wetlands Research
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• v.26 no.1
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• pp.72-81
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• 2024

Korea is surrounded by the sea on three sides, salt marshes and coastal sand dunes adjacent to them are developed. This study attempted to reveal the characteristics of the coastal ecosystem by investigating and analyzing the characteristics of plant communities and flora of 571stands of 48 sites in the salt marshes and coastal sand dunes on the western and southern coasts of Korea. As a result, in the salt marshes, 39 vegetation units appeared, with Phragmites australis community having the widest area and Chenopodium virgatum community having the narrowest area. Suaeda glauca community was distributed closest to the coastline, while Suaeda maritima community extended the farthest seaward. In the coastal sand dunes, 29 vegetation units were distributed with Elymus mollis community having the widest area and Salsola komarovii community having the narrowest area. Calystegia soldanella community was showed closest to the coastline, while Rosa rugosa community extended the farthest landward. The flora of the salt marshes consisted of 6 families, 14 genera, 17 species, 1 variant, and 18 taxa (floristic regional indicator score; 16) and the coastal sand dunes consisted of 11 families, 18 genera, 20 species, 1 variant, and 21 taxa (floristic regional indicator score; 34) appeared slightly higher than that of salt marshes. This result is interpreted that halophyte that are sensitive to salinity can grow on the coast of the west-south coast of Korea because coastal sand dunes are more indirectly affected by sea than salt marshes.

• Korean Journal of Soil Science and Fertilizer
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• v.46 no.6
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• pp.426-433
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• 2013

This study was aimed to classify soil desalination area for cultivation using NDVI (Normalized difference vegetation index) of high-resolution satellite image because the soil salinity affects the change of plant community in reclaimed lands. We measured the soil salinity and NDVI at 28 sites in the Saemangeum reclaimed land in June 2013. In halophyte and non-vegetation sites, no relation was found between NDVI and soil salinity. In glycophyte sites, however, we found that the soil salinity was below 0.1% and NDVI ranged from 0.11 to 0.57 which was greater than the other sites. So, we could distinguish the glycophyte sites from the halophyte sites and non-vegetation, and classify the area that soil salinty was below 0.1%. This technique could save the time and labor to measure the soil salinity in large area for agricultural utilization.

• KSBB Journal
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• v.31 no.4
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• pp.200-207
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• 2016

In the present study, antioxidant activity of crude extract and its solvent-partitioned subfractions (n-hexane, 85% aqueous methanol, n-butanol, and water) obtained from Atriplex gmelinii was investigated using several different antioxidant assays. The tested samples possessed different antioxidant and radical-scavenging activities in different assays. n-butanol fraction showed the most potent radical-scavenging activity on reducing power while 85% aqueous methanol fraction exhibited the highest radical-scavenging activity on DPPH radicals and intracellular reactive oxygen species (ROS). On the otherhand, n-BuOH and 85% aqueous methanol revealed the similar inhibitory effect on peroxynitrite-scavenging and genomic DNA oxidation. These results suggest that the Atriplex gmelinii can be used as the valuable source for developing a natural antioxidant.

• Journal of Plant Biotechnology
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• v.36 no.3
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• pp.236-243
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• 2009

The halophyte Leymus chinensis (Trin.) is a perennial rhizome grass (tribe Gramineae) that is widely distributed throughout China, Mongolia and Siberia. This study was conducted to investigate an optimal condition for plant regeneration from mature seeds, leaf base segments, and root segments in L. chinensis. Plant growth regulators affecting embryogenic callus induction and plant regeneration were investigated by four-factor-three-level [L9 (34)] orthogonal test in this study. The effects of explants types (mature seeds, leaf base segments and root segments), callus types, medium types were examined in this study. Wild type (WT) and Jisheng No. 1 plants (JS) were used for primary callus induction. A clear explants difference was seen during callus induction; mature seeds were considered as the preferred explants; and the highest frequency of callus induction was obtained in Medium 6 using mature seeds as explants in WT. Plant regeneration ability was evaluated by frequencies of green callus forming, shooting, rooting, and shooting with roots. Effect of α-naphthalene acetic acid (NAA) on shoot regeneration was remarkable with the highest frequency of 70.8% in WT after 2-month culture. The medium with 0.2- 0.5 mg/L NAA was found to have the highest shoot induction. All regenerated shoots were successfully rooted when transferred on half-strength Murashige and Skoog (MS) basal medium. The acclimatized plantlets were grown to mature with flowering and seeds setting in green house conditions.

• The Korean Journal of Ecology
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• v.6 no.3
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• pp.167-176
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• 1983

Species composition, life form, biomass and soil properties of the halophyte communities were investigated from July to September, 1982. At the reclaimed land of Sanho-ri, sand dune of Jido, salt marsh of Suncheon Bay, and Somjin River estuary of Baealdo, species numbers were 26, 14, 13 and 7, dominant species were Salicornia herbaceae, Carex pumila and Suaeda japonica, respectively. Species composition of the 4 investigated areas was 13 families, 25 genera and 39 species, and of them, 10 families, 21 genera and 24 species were attributed to halophytes. Out of 22 life forms, the representative for Jido was $ G-D_4-R_1-3-e.t$and those for the other sites were Th-G4-R5-e. Above ground biomass of all species for Sanho-ri, Jido, Suncheon Bay and Baealdo were 441.3, 202.0, 150.7 and 353.3 g.dw/m2 and the ratios of above ground biomass halophytes to all species were 93.5, 92.7, 90.8 and 100%, respectively. The leading dominant species formed a continuum according to the salt gradiant. The similarity between Baealo and Suncheon Bay was relatively high, and Jido was quite different from the others in species composition. Aster tripolium was stenohaline and appeared at the low salt concentration, but Suaeda maritima and Suaeda asparagoides were duryhaline and occured at the relatively high salt concentration.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.15 no.2
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• pp.57-75
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• 2012

This study was carried out to investigate the flora of estuary regions, Namdaecheon, Jusucheon, and Maeupcheon, placed in the east coast of Gangwon-do. The vascular plants were summarized as 441taxa, including 90 families, 277 genera, 388 species, 2 subspecies, 35 varieties, and 6 forms. Number of species in each investigated region was 271taxa of Namdaecheon, 247taxa of Jusucheon, and 285taxa of Maeupcheon, respectively. Among the investigated 441taxa, 7 Korean endemic, 5 rare plants and 37 specially designated plants by Ministry of Environment were also included. Aquatic and hydrophyte plants were collected 41taxa, and the thinophyte and halophyte plants were investigated 22taxa. The naturalized plants were 59taxa, and the percent of naturalized plant species was estimated as 13.3%. Based on the floristic composition, Maeupcheon was estimated as well conserved estuary region among the investigated 3 rivers.

• Journal of the East Asian Society of Dietary Life
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• v.20 no.3
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• pp.452-457
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• 2010

To obtain basic information on the utilization of Suaeda (S.) asparagoides $M_{IQ}$ as a raw material in food, it's chemical components of were investigated. S. asparagoides $M_{IQ}$ from the Hwaseong-si in Korea was used after drying and grinding with powder. The total dietary fiber, insoluble dietary fiber and soluble dietary fiber contents of S. asparagoides $M_{IQ}$ were found to be 39.41%, 37.63% and 1.78%, respectively. Macromineral components of S. asparagoides $M_{IQ}$ were Na (7.6%), K (2.4%) and Mg (0.6%). High contents of microminerals such as Cu, Mn and Zn were also found. Lysine and methionine which are essential amino acids were detected with the value of 215 mg/100 g and 23.2 mg/100 g, respectively. S. asparagoides $M_{IQ}$ that was harvested in May was higher in crude protein than that harvested in September but the crude fat content of that which was harvested in September was twice as high as that harvested in May.