• Journal of Life Science
• /
• v.21 no.3
• /
• pp.358-367
• /
• 2011

Soybean is known to contain various phytochemicals that are related to anti-oxidant, anti-inflammatory and anti-obesity effects in mice and humans. The anti-obesity effect of by-product extracts from soybean on the differentiation of 3T3-L1 pre-adipocytes to adipocytes was investigated by suppressing adipocyte differentiation and lipid accumulation with Oil Red-O assay and quantitative PCR. In inducing differentiation of 3T3-L1 pre-adipocytes in the presence of an adipogenic cocktail, isobutylmethylanthine (IBMX), dexamathasone, and insulin, treatment with filtrated soybean soaked water, soybean milk, and soycurd residue from soybean curd processing significantly decreased mRNA expression of obesity-related gene such as PPAR${\gamma}$, Fabp4, and Scd1, adipsin, apolipoprotein (APOE) and adiponectin (ADIPOQ) without any significant cytotoxicity. We also determined the well-known isoflavones in soybean, such as daidzein and genistein, in the by-product extracts. Taken together, we suggest that soybean by-product extract showed anti-obesity effect by suppressing adipocyte related gene expression, and that by-products collected during soybean curd processing may be a good candidate as an ingredient in health care products.

• The Korean Journal of Mycology
• /
• v.44 no.4
• /
• pp.377-381
• /
• 2016

Abnormal chloranthy was observed on Prunus mume trees grown in an orchard in Uiseong, Gyeongbuk province, Korea in 2015. Flower buds showing chloranthy were covered with numerous orange-colored sori, which were confirmed to contain spermogonia and aeciospores by stereo microscopy and light microscopy. When observed using scanning electron microscope, the aeciospores were globose, ellipsoid, or ovoid, and measured $22.8{\sim}35.6{\times}19.8{\sim}36.8{\mu}m$, with a verrucae wall ornamentation. To construct a phylogeny, the large and small subunit regions of rDNA were sequenced and compared with those of allied species by the maximum likelihood method. The isolated fungus species showed high similarities with Caeoma makinoi (Teleomorph: Blastospora smilacis), which was previously isolated from P. mume in Japan. Therefore, we initially report this fungal agent parasitic on P. mume as C. makinoi in Korea and name the disease it causes as "flower bud chloranthy disease".

• 한국균학회소식:학술대회논문집
• /
• 2014.10a
• /
• pp.34-34
• /
• 2014

Filamentous fungi of the genus Colletotrichum (teleomorph, Glomerella) are considered major plant pathogens worldwide. Cereals, legumes, vegetables, and fruit trees may be seriously affected by this pathogen (1). Colletotrichum species cause typical disease symptoms known as anthracnoses, characterized by sunken necrotic tissue, where orange conidial masses are produced. Anthracnose appears in both developing and mature plant tissues (2). We investigated disease occurrence in apple orchards from 2013 to 2014 in northern Gyeongbuk province, Korea. Typical anthracnose with advanced symptoms was observed in all apple orchards studied. Of late, static fruit spot symptoms are being observed in apple orchards. A small lesion, which does not expand further and remains static until the harvesting season, is observed at the beginning of fruit growth period. In our study, static symptoms, together with the typical symptoms, were observed on apples. The isolated fungus was tested for pathogenicity on cv. 'Fuji apple' (fully ripe fruits, unripe fruits, and cross-section of fruits) by inoculating the fruits with a conidial suspension ($10^5$ conidia/ml). In apple inoculated with typical anthracnose fungus, the anthracnose symptoms progressed, and dark lesions with salmon-colored masses of conidia were observed on fruit, which were also soft and sunken. However, in apple inoculated with fungi causing static symptoms, the size of the spots did not increase. Interestingly, the shape and size of the conidia and the shape of the appressoria of both types of fungi were found to be similar. The conidia of the two types of fungi were straight and cylindrical, with an obtuse apex. The culture and morphological characteristics of the conidia were similar to those of C. gloeosporioides (5). The conidia of C. gloeosporioides germinate and form appressoria in response to chemical signals such as host surface wax and the fruitripening hormone ethylene (3). In this study, the spores started to germinate 4 h after incubation with an ethephon suspension. Then, the germ tubes began to swell, and subsequently, differentiation into appressoria with dark thick walls was completed by 8 h. In advanced symptoms, fungal spores of virtually all the appressoria formed primary hyphae within 16 h. However, in the static-symptom fungus spores, no primary hyphae formed by 16 h. The two types of isolates exhibited different growth rates on medium containing apple pectin, Na polypectate, or glucose as the sole carbon. Static-symptom fungi had a >10% reduction in growth (apple pectin, 14.9%; Na polypectate, 27.7%; glucose, 10.4%). The fungal isolates were also genetically characterized by sequencing. ITS regions of rDNA, chitin synthase 1 (CHS1), actin (ACT), and ${\beta}$-tubulin (${\beta}t$) were amplified from isolates using primer pairs ITS 1 and ITS 4 (4), CHS-79F and CHS-354R, ACT-512F and ACT-783R, and T1 and ${\beta}t2$ (5), respectively. The resulting sequences showed 100% identity with sequences of C. gloeosporioides at KC493156, and the sequence of the ${\beta}$t gene showed 100% identity with C. gloeosporioides at JX009557.1. Therefore, sequence data from the four loci studied proves that the isolated pathogen is C. gloeosporioides. We also performed random amplified polymorphic DNA-PCR, which showed clearly differentiated subgroups of C. gloeosporioides genotypes. The clustering of these groups was highly related to the symptom types of the individual strains.

• Korean Journal of Human Ecology
• /
• v.20 no.4
• /
• pp.861-869
• /
• 2011

This paper examined the frequency of skipping breakfast by middle school students for one week. It carried out the study in order to understand the ways skipping breakfast effected other habits. The subjects were 467 middle school students (219 boys, 248 girls). The results of the survey were as follows; average male students were $171.16{\pm}5.9cm$, $61.45{\pm}10.0kg$, and $20.93{\pm}2.9$ in height, weight and body mass index, while the average female students were $159.27{\pm}6.0cm$, $51.62{\pm}7.9kg$ and $20.33{\pm}2.8$. As for the reasons for skipping breakfast, 58.0% of the subjects responded that they had no time. The frequency of skipping breakfast was divided into the eating group (0~1 time) and the skipping breakfast group (2~3times/week, 4~5 times/week, or 6~7 times/week). As the frequency of skipping breakfast increased, so the rate of the subjects eating alone was higher and eating with family lower(p<.05). The dietary behavior change stage was divided into contemplation stage, preparation stage, action stage, and maintenance stage. The eating group(59.2%) was higher than the skipping breakfast group(29.1%~46.3%) in the rate of subjects maintenance stage(p<.01). Accordingly, skipping breakfast was closely connected with eating habits in their families. A dietitian needs to teach the importance of meals and life habits, so as to enhance public awareness of health and nutrition to the students. Subjects of nutrition education must even enlarge their parents as well as the students.

• Korean journal of applied entomology
• /
• v.43 no.1
• /
• pp.15-20
• /
• 2004

Leaf damage by apple leafminer, Lyonetia prunifoliella, was surveyed on Fuji apple orchards with seedling rootstock from 1992 to 2001 and with M.9 rootstock from 1999 to 2001. The damage was negligible in apple orchards with seedling rootstock as 0.15% in 1992 and 0.06% in 1993. However it sharply increased to more than 3.39% since 1994, reaching at peak damage of 6.28% to 7.78% in 1996 to 1998. Damages on apple orchards with M.9 rootstock from 1999 to 2001 were higher as 6.09% to 15.3%, compared with those with seedling rootstock in the same years. In seedling rootstock apple, the damage was highest on september but in M.9 rootstock apple, the difference was not significant. Leaf damage showed no differences between cultivars including 4 Korean cultivars and Fuji. These results may explain that increase of M.9 rootstock apple orchards is responsible for the recent outbreak of the leafminer in Korea.

• Korean journal of applied entomology
• /
• v.44 no.2
• /
• pp.151-156
• /
• 2005

The development of resistance to bifenazate (resistance ratio of egg=40.3 folds) was found in population of the twospotted spider mite, Tetranychus urticae, collected from rose greenhouses in Chilgok, Gyeongbuk Province in December 2000. This population was selected for 4 years with bifenazate treatment (over 150 times), and showed 248.8 folds increase in resistance as compared to susceptible (S) strain. Inheritance of bifenazate resistant strain (R) and cross resistance of this strain to 9 acaricides were investigated. There were differences of susceptibility in the bifenazate concentration-mortality relationships in F1 progenies obtained from reciprocal cross with the S and R strain $(R{\times}S,\;S{\times}R)$. Degrees of dominance were 0.48 and 0.94 in adult females and eggs of $R{\times}S$ and -0.85 and -0.17 in adult females and eggs of $S{\times}R$, respectively. Inheritance type in the F1 progeny of $R{\times}S$ was incomplete dominant, and F1 progeny of $S{\times}R$ was incomplete recessive. These results suggest that inheritance of bifenazate resistance is controlled by a complete dominance. The R strain exhibited cross resistance to acequinocyl and fenpyroximate in adult females, and amitraz, emamectin benzoate, fenpyroximate, milbemectin, pyridaben and spirodiclofen in eggs. However they showed negatively correlated cross-resistance to emamectin benzoate and milbemectin in adult females, and abamectin in eggs.

• Journal of dental hygiene science
• /
• v.10 no.5
• /
• pp.329-334
• /
• 2010

This study was performed to identify the relationship between scaling behaviors and knowledge about scaling and oral health belief of worker, and to provide baseline data for developing industry oral health policy. The survey had conducted self-administered questionnaires by 326 workers in Gyeongsan and Yeongcheon areas in Gyeongbuk province. Their scaling behaviors, knowledge and oral health belief were measured. The results were as follow; The average score of knowledge about scaling was 6.44. The average oral health belief was 2.72. In detail, 'usefulness' was 3.24 as the highest and 'seriousness' was 2.25 as the lowest. The periodic scaling and scaling cycle were associated with 'seriousness' and 'barrier' as positive correlation(p<.05, .01). On the other hand, they were associated with knowledge and 'usefulness' as negative correlation(p<.01). Meanwhile, scaling motive showed positive correlation with 'cue of action'(p<.01), showed negative correlation with knowledge(p<.01). In the correlation between knowledge about scaling and oral health belief, knowledge showed negative correlation with 'barrier'(p<.01) and showed positive correlation with 'usefulness'(p<.01). Finally, we need to advertise and manage the importance of scaling politically and makes people do the voluntary scaling in six-month intervals in order to keep the periodontal health of worker.

• Journal of Life Science
• /
• v.20 no.5
• /
• pp.639-643
• /
• 2010

Sudden deaths have occurred in feedlot cattle with marked necro-hemorrhagic enteritis of the jejunum, ileum and colon. Suckling beef calves are the most frequently affected. Over-consumption of large amounts of milk, inadequate colostrum intake, chilling and stress are conducive to the development of enterotoxemia. Enterotoxemia caused by Clostridium perfringens type D mostly occurs following a sudden change of diet, particularly to feeds made richer in order to grow the cattle to market weight in feedlots. During July 2006, sudden deaths of cattle occurred in the Youngcheon regional area of Gyeongbuk province. There were no significant clinical signs except anorexia, depression, intermittent diarrhea and mild respiratory failure. Histological findings revealed a prominent intranuclear inclusion as well as infiltration of the globular leukocytes in various organs including the heart, kidneys, liver, spleen and lymph nodes. Spleen and lymphatic tissues showed lymphatic necrosis and a starry sky appearance. In the submucosa of the small intestines, basophilic aggregation was detected with massive infiltration of the globular leukocytes and eosinophils. Gram staining for the tissue sections containing inclusions of the small intestines revealed a positive histochemical reaction. Taken together, we suggest that Clostridium perfringens type D-induced enterotoxemia is determined to be the cause of sudden death of feedlot cattle.

• Weed & Turfgrass Science
• /
• v.4 no.2
• /
• pp.77-84
• /
• 2015

We surveyed the distribution pattern of weeds in onion, garlic, potato, and barley fields including 304 sites of Gyeongsangbuk-do. The weeds were summarized as 30 family and 125 species in onion crop field, 29 family and 101 species in garlic field, 30 family 88 species in potato field, finally 27 family and 108 species. Compositae was dominant family (26.4%, 33 species), followed by Polygonaceae (8.8%, 11 species), Cruciferae (8.8%, 11 species) in onion field. Compositae was also dominant family (24.8%, 25 species) in garlic field, it was 22.7% (20 species) in potato field, and it was also 24.1% (26 species) in barley field, respectively. Among these winter crops, major five families were occupied 61.1, 58.4, 58.0 and 57.5% in turn at barley, onion, potato and garlic fields. The PCA-covariance plot analysis for investigation of occurrence pattern of weeds by four winter crop fields revealed that the occurrence pattern of weed species in barley field was distinguished by Alopecurus aequalis var. amurensis, Stellaria alsine var. undulate and Stellaria aquatica.

• Journal of Korean Society of Forest Science
• /
• v.99 no.4
• /
• pp.597-602
• /
• 2010

The most basic matter to establish forest carbon circulation village is statistic on greenhouse gas emissions. But currently, although there is statistic on greenhouse gas emissions in the level of city or province, there is not statistic on greenhouse gas emission in village unit. According to the results, The model area is located in Seobyeok-ri, Chunyang-myeon, Bonghwa-gun, Gyeongsangbuk-do, the total $CO_2$emissions caused by energy used in the model area was $1,755tCO_2$. Heating accounts for 55% of total emissions followed by 23% for power and 22% for vehicles. The model area emitted $572tCO_2$ due to rice growing and livestock raising, accounting for approximately 24.5% of total $CO_2$ emissions. It is expected that a reduction of as much as $884tCO_2$ emissions will be made from the current $964tCO_2$ to a level of 1/12th that amount, or $80tCO_2$ by replacing heating energy currently used in the model area with wood bioenergy such as wood chips or pellets. In addition, carbon emission reduction is expected for both heating and power by replacing the power consumption in houses, buildings, and street lights with solar power.