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Flower Bud Chloranthy Disease Caused by Caeoma makinoi on Prunus mume

Caeoma makinoi에 의한 매실나무 꽃눈녹병의 발생 보고

  • Lee, Seung-Yeol (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Lee, Yeon-Hee (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Park, Su-Jin (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Lee, Han-Dong (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Lee, Jae-Jin (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Kang, In-Kyu (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Lee, Hyang Burm (College of Agriculture and Life Sciences, Chonnam National University) ;
  • Jung, Hee-Young (College of Agriculture and Life Sciences, Kyungpook National University)
  • 이승열 (경북대학교 농업생명과학대학) ;
  • 이연희 (경북대학교 농업생명과학대학) ;
  • 박수진 (경북대학교 농업생명과학대학) ;
  • 이한동 (경북대학교 농업생명과학대학) ;
  • 이재진 (경북대학교 농업생명과학대학) ;
  • 강인규 (경북대학교 농업생명과학대학) ;
  • 이향범 (전남대학교 농업생명과학대학) ;
  • 정희영 (경북대학교 농업생명과학대학)
  • Received : 2016.12.01
  • Accepted : 2016.12.10
  • Published : 2016.12.30

Abstract

Abnormal chloranthy was observed on Prunus mume trees grown in an orchard in Uiseong, Gyeongbuk province, Korea in 2015. Flower buds showing chloranthy were covered with numerous orange-colored sori, which were confirmed to contain spermogonia and aeciospores by stereo microscopy and light microscopy. When observed using scanning electron microscope, the aeciospores were globose, ellipsoid, or ovoid, and measured $22.8{\sim}35.6{\times}19.8{\sim}36.8{\mu}m$, with a verrucae wall ornamentation. To construct a phylogeny, the large and small subunit regions of rDNA were sequenced and compared with those of allied species by the maximum likelihood method. The isolated fungus species showed high similarities with Caeoma makinoi (Teleomorph: Blastospora smilacis), which was previously isolated from P. mume in Japan. Therefore, we initially report this fungal agent parasitic on P. mume as C. makinoi in Korea and name the disease it causes as "flower bud chloranthy disease".

2015년 5월, 경상북도 의성군 소재 매실과원에서 비정상적인 엽화증상이 매실나무에서 관찰되었다. 관찰된 엽화증상에서는 외부에 주황색의 돌기가 관찰되었고, 실체현미경으로 관찰한 결과 녹포자퇴로 확인되었다. 자세한 형태를 관찰하기 위해 광학현미경과 주사전자현미경으로 관찰한 결과, 녹포자는 구형, 타원형 또는 난형이었고, 약 $22.8{\sim}35.6{\times}19.8{\sim}36.8{\mu}m$ 크기를 가지면서 표면은 무사마귀형으로 관찰되었다. 녹포자의 정확한 동정과 계통학적 유연관계 분석을 위하여, 18S rDNA와 large subunit rDNA 영역을 증폭시킨 후 염기서열을 분석하고 계통학적 유연관계를 분석하였다. 그 결과, 매실나무의 엽화증상에서 관찰된 병원균은 일본에서 분리된 Caeoma makinoi (Teleomorph: B. smilacis) 균주와 100% 일치하는 것으로 확인되었다. 따라서 본 연구에서는 국내 매실나무에서 엽화증상을 일으키는 녹병균을 Caeoma makinoi로 보고하고, 위 병원균에 의한 매실나무 엽화증상을 매실꽃눈녹병으로 제안하고자 한다.

Keywords

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