• Title/Summary/Keyword: Gun-Ho Song

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Analysis of Varietal Differences in Pre-harvest Sprouting of Rice using RNA-Sequencing (RNA-Sequencing을 이용한 벼 품종간 수발아 차이 분석)

  • Choi, Myoung-Goo;Lee, Hyen-Seok;Hwang, Woon-Ha;Yang, Seo-Yeong;Lee, Yun-Ho;Lee, Chung-gun;Yun, Song Joong;Jeong, Jae-Hyeok
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.65 no.4
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    • pp.274-283
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    • 2020
  • Seed dormancy is an adaptive trait in which seeds do not germinate under unfavorable environmental conditions. Low dormancy seeds are easily germinated under optimal environmental conditions, and these characteristics greatly reduce the yield and quality of crops. In the present study, we compared the pre-harvest sprouting (PHS) rate of two cultivars, Joun and Jopyeong, using the Winkler scale after heading day and temperature of the test. The PHS rate increased as the Winkler scale after heading day increased from 700℃ to 1100℃ and the temperature of the test increased. In all conditions, the PHS rate of Jopyeong was higher than that of Joun. RNA-sequencing was used to analyze the cause of the high PHS rate. We analyzed the biological metabolic processes related to the abscisic acid (ABA) metabolite pathway using the KEGG mapper with selected differentially expressed genes in PHS seeds. We found that the expression of ABA biosynthesis genes (OsNCEDs) was down-regulated and that ABA catabolic genes (OsCYP707As) was up-regulated in PHS seeds. However, the quantitative real-time PCR results showed that Joun had a higher expression of OsNCEDs than that of Jopyeong, but OsCYP707As did not yield a significant result. Joun displayed higher ABA content than that of Jopyeong not only during ripeness time but also during PHS treatment. Taken together, we provided evidence that the ABA content remaining in the seed is important to the PHS rate, which is determined by the expression level of the ABA biosynthesis gene OsNCEDs.

Therapeutic Effect of 30 Cases of Scalp and Do-ma Acupuncture Related with Governor Vessel(Dok) and Bladder Meridian(Chok-taeyang) on Acute Low Back Pain (급성(急性) 요부(腰部) 염좌(捻挫) 환자 30례(例)의 두부(頭部) 독맥경(督脈經), 족태양방광경(足太陽膀胱經) 경혈(經穴) 도마침법(倒馬鍼法) 치료효능에 대한 임상적(臨床的) 고찰(考察))

  • Kang, Min-Wan;Kim, Sung-Lae;Choi, Ka-Wan;Song, Hyong-Gun;Kim, Jeong-Ho;Yang, Ki-Young;Yim, Yun-Kyoung;Hong, Kwon-Eui;Lee, Hyeon;Kim, Young-Il
    • Journal of Acupuncture Research
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    • v.23 no.3
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    • pp.207-214
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    • 2006
  • Objectives : This study is designed in order to evaluate the therapeutic effect of Scalp Acupuncture and Do-ma Acupuncture related with Governer Vessel(Dok) and Bladder Meridian(Chok-taeyang) on acute low back pain Methods : We investigated 30 patients suffering from acute low back pain were admitted to Dunsan O.M.hospital from March. 1st 2005 to December. 30th 2005. We divided into two groups: one group was treated with Scalp acupunture, another group was treated with common acupuncture. 30 patients, were diagnosed only straightened curvature on lateral view of lumbar spine X -ray. Scalp acupunture related with Governer Vessel(Dok) and Bladder Meridian(Chok-taeyang) and Do-ma acupuncture. To estimate the efficacy of treatment that applied for two groups, we compared the visual analog scale(VAS), Oswestry disabiliby index(ODI) and Straight Leg Raising Test(SLRT) score of two groups statistically. Results : Both Scalp acupuncture related with Governer Vessel(Dok) and Bladder Meridian (Chok-taeyang) and Do-ma acupuncture therapy showed good effect on acute low back pain. It was proved by the difference between VAS, ODI and SLRT score checked before treatment and what checked after treatment on each groups. Conclusion: The therapeutic effect of Scalp Acupuncture and Do-ma Acupuncture on acute low back pain can be recommended as a useful therapy to treat acute low back pain.

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A Establishment of Visual Component Elements and Investigation Analysis for a House-Gates in Rural Villages (농촌마을 주택대문의 경관구성요소 설정 및 실태파악 - 충남 청양군을 중심으로 -)

  • Lee, Gyeong-Jin;Cho, Soung-Ho;Song, Byeong-Hwa
    • Journal of Korean Society of Rural Planning
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    • v.13 no.3
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    • pp.83-90
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    • 2007
  • The purpose of main entrance was to protect the house with the wall and working for the house-gate where people come in and go out. On the other hand, the type of main entrance is changed variously depended on the materials for house and method of construction. Eleven villages in the Chungyang-Gun where the environment of rural villages is well maintained was chosen and researched to make data. These data of visual landscape elements were analysed by using the SPSS 12.0 for Windows. Relations of the visual component elements were analysed by the analysis for frequency and analysis for crosstab. From the above research we could conclude below results. Through review of the pre-researching and researching literatures, 11 types of visual component elements were selected such as the types of the gate, the looking through degree of the gate, the material of the gate, the color of the gate, the plan of the house, the material of the roof, a material of the wall, a color of the wall, the form of the penetration, the area to put, the area of a site, the year of the constructing a building. For the types of the gate, the gate from without the roof has been the most popular since it appeared 41.5%. For the looking through degree of the gate, a complete blockade form has been the most popular since it appeared 63.2%. For the material of the gate, the iron plate has been the most popular since it appeared 32.1%. For the color of the gate, color has been the most popular since it appeared 68.4%. For the plan of the house, 'ㄷ' form has beneath most popular since it appeared 38.3%. For the material of the roof. The Zinc has been the most popular since it appeared 51.9%. For a material of the wall, A cement mortar has been the most popular since it appeared 47.7%. For a color of the wall, Without tile color has hem the most popular since it appeared 67.0%. For the form of the penetration. The plain form has been the most popular since it appeared 54.0%. For tile area of a site, $100m^2{\sim}200m^2$ has been the most popular since it appeared 39.0%. The results of this study can provide to use of indicates four rural-housing reform. From now on, the results look forward to offering the meaning directions for the improvement of rural house gates.

Environmental Impact Assessment of Agricultural Systems Using the Life Cycle Assessment (전과정평가 도입을 통한 농업환경영향 평가)

  • Shim, Kyo-Moon;Jeong, Ji-Sun;So, Kyu-Ho;Lim, Song-Tak;Roh, Kee-An;Kim, Gun-Yeob;Jeong, Hyun-Cheol;Lee, Deog-Bae
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.2
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    • pp.237-241
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    • 2010
  • Many policies have been implemented to mitigate the greenhouse gases in atmosphere overall of sectors. With considering the distinct characteristics of the food security, agricultural sector is no exception to this situation. To this regard, total amount of carbon which is emitted through all of the agricultural production process is calculated, and being based on this result, the demand for the introduction of agricultural production system with low carbon has been rising. Case studies on the application of life cycle assessment (LCA) technique to agricultural sector are found in many countries. For example, life cycle inventory (LCI) data bases of crop, farm infrastructure, fertilizer, farm machinery, and etc., have been constructed and provided by Ecoinvent (Swiss centre for life cycle inventories) of Swiss. In Japan, Top-down typed LCA methodology for agriculture is developed based on the inter-industry analysis, and is evaluated according to the productive method of crop. On the other hand, environmental impact assessment of agricultural system using LCA in Korea is just in the beginning stages. So it is required to assess environmental impact on agricultural fertilizer and pesticide, and to develop their flow modeling, and methodology of LCA of agricultural sector. Environmental impact assessment on agricultural materials, machinery, and infrastructure will also be carried out.

Residual Radioactivity Investigation & Radiological Assessment for Self-disposal of Concrete Waste in Nuclear Fuel Processing Facility (콘크리트 폐기물의 자체처분을 위한 잔류방사능 조사 및 피폭선량평가)

  • Seol, Jeung-Gun;Ryu, Jae-Bong;Cho, Suk-Ju;Yoo, Sung-Hyun;Song, Jung-Ho;Baek, Hoon;Kim, Seong-Hwan;Shin, Jin-Seong;Park, Hyun-Kyoun
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.5 no.2
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    • pp.91-101
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    • 2007
  • In this study, domestic regulatory requirement was investigated for self-disposal of concrete waste from nuclear fuel processing facility. And after self-disposal as landfill or recycling/reuse, the exposure dose was evaluated by RESRAD Ver. 6.3 and RESRAD BUILD Ver.3.3 computing code for radiological assessments of the general public. Derived clearance level by the result of assessments for the exposure dose of the general public is 0.1071Bq/g (3.5% enriched uranium) for landfill and $0.05515Bq/cm^2$ (5% enriched uranium) for recycling/reuse respectively. Also, residual radioactivity of concrete waste after decontamination was investigated in this study. The result of surface activity is $0.01Bq/cm^2\;for\;{\alpha}-emitter$ and the result of radionuclide analysis for taken concrete samples from surface of concrete waste is 0.0297Bq/g for concentration of $^{238}U$, below 2w/o for enrichment of $^{235}U$ and 0.0089Bq/g for artificial contamination of $^{238}U$ respectively. Therefore, radiological hazard of concrete waste by self-disposal as landfill and recycling/reuse is below clearance level to comply with clearance criterion provided for Notice No.2001-30 of the MOST and Korea Atomic Energy Act.

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A Study on Single Dose Toxicity of Intravenous Injection of Mecasin Herbal Acupuncture

  • Lee, Seong Jin;Jeong, Ho Hyun;Lee, Jong Chul;Cha, Eun Hye;Park, Man Yong;Song, Bong Gun;Son, Il Hong;Kim, Sung Chul
    • Journal of Acupuncture Research
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    • v.33 no.1
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    • pp.1-7
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    • 2016
  • Objectives : This study was conducted to analyze the single dose toxicity of Mecasin (Gami-Jakyak Gamcho buja Decoction) herbal acupuncture administered in the vein of Sprague-Dawley rats. Methods : All experiments were performed at the Medvill, an institution licensed to conduct nonclinical studies, under the Good Laboratory Practice (GLP) regulations. Sprague-Dawley rats were chosen in this pilot study. In the experiment, Sprague-Dawley rats were divided into four groups of five male and five female animals per group. Doses of Mecasin herbal acupuncture, at 0, 500, 1,000, and 2,000 mg/kg, were given to the experimental groups, and a dose of normal saline solution, at 2 ml/kg, was administered to the control group. Mecasin herb acupuncture and normal saline were injected into the vein at once, and we observed mortality, clinical signs, weights, and gross findings for 14 days after injection. This study was conducted under the approval of the Institutional Animal Ethics Committee. Results : There is no death or abnormality in any of the four groups. All groups put on weights favorably. There are no significant gross findings in necropsy examinations. Conclusions : The above results showed that intravenous injection of 500-2,000 mg/kg of Mecasin herb acupuncture did not cause any changes in weight or, in the results of necropsy examinations, in mortalities. Therefore, the toxicity of Mecasin herb acupuncture was not confirmed, and the presumptive lethal dose of Mecasin herb acupuncture was higher than 2,000 mg/kg. The outcomes suggest that treatment with Mecasin herbal acupuncture is relatively safe. Further evaluations on this subject are needed to yield more concrete evidence.

Ozone Inhalation with 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)- 1-butanone and/or Dibutyl Phthalate Induced Cell Cycle Alterations via Wild-type p53 Instability in B6C3F1 Mice

  • Kim, Min-Young;Song, Kyung-Suk;Park, Gun-Ho;Kim, Hyun-Woo;Park, Jin-Hong;Kim, Jun-Sung;Jin, Hwa;Kook-Jong, Eu;Cho, Hyun-Sun
    • Toxicological Research
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    • v.20 no.1
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    • pp.71-82
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    • 2004
  • Changes in cell cycle control in the lungs and liver of the B6C3F1 mice (20 males per each group) exposed to ozone (0.5 ppm), 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK, 1.0 mg/kg), and dibutyl phthalate (DBP, 5,000 ppm) after 52 weeks were examined through Western, Northern blot, and immunohistochemistry based on alterations in protein expression levels of G1/S checkpoints (cyclin D1, cyclin E, and PCNA), G2/M checkpoints (cyclin B1, cyclin G, and cyclin A), negative regulators (p53, p21, GADD45, and p27), and positive regulator (mdm2). Expression levels of cyclins D1, E, G, PCNA, mutant p53, and mdm2 proteins were higher in the lungs and livers treated with combination of toxicants than in those treated with ozone only. Expression levels of the wild-type and mutant p53, p21, GADD45, p27, and mdm2 proteins and mRNAs were higher in toxicant-treated groups than those of the control. Immunohistochemical analysis revealed staining intensities of the PCNA, cyclin D1, c-myc and mdm2 protein- treated lungs and livers were stronger than those of the control group. Our results showed that combined treatment of ozone with NNK/DBP altered the cell cycle control through instability of the wild-type p53 gene. Such pivotal p53-mediated cell cycle alterations may be responsible for the toxicity observed under our experimental condition. These results may be applied to risk assessment of mixture-induced toxicity.

Core Issues and Tariff Reduction of Timber Products in Non-Agricultural Market Access on WTO/DDA in Korea (WTO/DDA협상 비농산물시장접근분야의 목재류의 주요 쟁점 및 관세 감축 영향)

  • Lee, Seong Youn;Jung, Byung-Heon;Song, Young Gun;Kim, Se Bin;Kwak, Kyung Ho
    • Journal of Korean Society of Forest Science
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    • v.97 no.4
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    • pp.408-416
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    • 2008
  • This study was analyzed the changes of the tariff reduction for the timber products according to the modalities proposed by the chairman of Non-agriculture Market Access. The resulted data would be expected to be used for establishing the strategies for the WTO/DDA negotiation of Korea. As WTO/DDA negotiation was officially resumed in the early 2007. It was necessarily required to develop a strategy how to address debating core issues raised during the negotiation since 2006. For this purpose, major core issues and agenda were arranged and analyzed in this study. In Korea, the results of the analysis show that, in the position of developed members, the applicable tariff rates after the tariff reduction of sawnwood and veneer sheet was not different from that in 2007. However, in the position of developing members, the applicable tariff rate of sawnwood and veneer sheet was increased after the tariff reduction. The unbound tariff lines, wood-based panels such as plywood and medium-density fiberboard, the applicable tariff rate after the tariff reduction was analyzed to be reduced more than 50% in the position of developed members, and therefore is determined to be considerable influenced upon the applicable tariff rates. On the other hand, in the position of developing country the tariff rates after the tariff reduction was higher than that of the applicable tariff rates in 2007. Thus no changes of the tariff reduction by a negotiation agreement was analyzed to be happened.

The Effect of Chinemys reevesii Gray Pharmacopuncture for Women with Knee Osteoarthritis (구판(龜板)약침이 여성의 퇴행성 슬관절염에 미치는 영향)

  • Shin, So Yeon;Seo, Dong Kyun;Kim, Shin Young;Seo, Jong Cheol;Seo, Yeon Ju;Lee, Yoon Joo;Bin, Chang Hyun;Jung, Taek Gun;Song, Chun Ho;Yoon, Hyun Min;Kim, Cheol Hong;Jang, Kyung Jeon
    • Journal of Acupuncture Research
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    • v.32 no.3
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    • pp.163-173
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    • 2015
  • Objectives : The aim of this study is to determine whether Chinemys reevesii Gray pharmacopuncture is a clinically effective treatment for women with knee osteoarthritis. Methods : Female patients with knee osteoarthritis were recruited from May $1^{st}$, 2014 to April $15^{th}$, 2015 at the Department of Acupuncture & Moxibustion Medicine of Dong-Eui University Korean Medical Hospital. Patients were randomly assigned to two groups. 4.0 ml of Chinemys reevesii Gray pharmacopuncture was injected at acupuncture points of the experimental group(n = 24) twice a week for three weeks. The control group(n = 24) received 5 % dextrose injections in order to identically stimulate the same acupuncture points of $ST_{35}$, $ST_{34}$, $SP_9$, $SP_{10}$, $GB_{34}$, $LR_8$, $EX-LE_4$, and $EX-LE_2$. The Short-Form McGill Pain Questionnaire(SF-MPQ), the Korean Western Ontario and McMaster Universities Osteoarthritis Index(KWOMAC), and the European Quality of Life-5 Dimensions(EQ-5D) were recorded to evaluate treatment efficacy at first visit, after two weeks and four weeks from the initial visit. Results : 40 patients completed the trial. The experimental group showed more significant improvement than the control group in the scores of SF-MPQ, KWOMAC, and EQ-5D. However, there was no significant difference between groups in a few of the assessment scales. Conclusions : Chinemys reevesii Gray pharmacopuncture can be an effective treatment in control of pain, improvement in function and health-related quality of life for women with knee osteoarthritis.

A Study for the Necessity of Terminology Standardization in Chuna Medicine (추나의학 용어 표준화 필요성 연구)

  • Kweon, Jeong-Ju;Kim, Min-Woo;Park, Kyung-Moo;Jang, Gun;Cho, Hyun-Cheul;Nam, Hang-Eoo;Shin, Byung-Cheul;Lim, Hyung-Ho;Song, Yun-Kyung
    • The Journal of Churna Manual Medicine for Spine and Nerves
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    • v.7 no.1
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    • pp.1-13
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    • 2012
  • Objectives: Although chuna medicine has progressed distinguishingly, yet chuna medical terminology hasn't been standardized. So there are a lot of difficulties in translating chuna related book and their meaning cannot be conveyed properly. For this reason, we could say standardization of chuna medical terminology is very essential. Purpose of our study was to develope a standard database of concept terms for chuna medicine, in addition, we considered establishing fundamental principles of chuna medical terminology. Methods: To select standard chuna medical terms, we sorted important chuna medical index words. Then we sorted those words into a group that has same meanings and united to one single term. In the meantime, we extracted index words from 26 domestic and foreign manual technique related books and sorted them out and based on these word, we translated chuna medical terms to Korean terms. In the case of chuna technique terms, we searched chuna text books for term those were wrongly used, and corrected them by suggesting fundamental principles of terminology. Results: 664 chuna words were selected as standard chuna terms and have been translated to English terms. In the process, adscititious words such as anatomical terms and title of books were exempted and selected only important words that could be used as index of chuna terms. In deciding essential elements of chuna technique terms, patient position, contact point, segmental contact point, malposition, procedure method were selected. Conclusions: Correcting chuna medical terms in a sort period could cause confusion, but in long term perspective, in the aspect of conveying the meaning clearly and education purpose, standardizing of chuna medical terminology must be done. From this study, standardization of chuna medical terms were chosen in large category, but further studies must be followed in order to standardize terms of subdivisional categories.

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