KOREAN JOURNAL OF CROP SCIENCE (한국작물학회지)

The Korean Society of Crop Science (한국작물학회)
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Analysis of Varietal Differences in Pre-harvest Sprouting of Rice using RNA-Sequencing

RNA-Sequencing을 이용한 벼 품종간 수발아 차이 분석

  • Choi, Myoung-Goo (National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Hyen-Seok (National Institute of Crop Science, Rural Development Administration) ;
  • Hwang, Woon-Ha (National Institute of Crop Science, Rural Development Administration) ;
  • Yang, Seo-Yeong (National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Yun-Ho (National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Chung-gun (National Institute of Crop Science, Rural Development Administration) ;
  • Yun, Song Joong (Department of Crop Science & Biotechnology, Chonbuk National University) ;
  • Jeong, Jae-Hyeok (National Institute of Crop Science, Rural Development Administration)
  • 최명구 (농촌진흥청 국립식량과학원) ;
  • 이현석 (농촌진흥청 국립식량과학원) ;
  • 황운하 (농촌진흥청 국립식량과학원) ;
  • 양서영 (농촌진흥청 국립식량과학원) ;
  • 이윤호 (농촌진흥청 국립식량과학원) ;
  • 이충근 (농촌진흥청 국립식량과학원) ;
  • 윤성중 (전북대학교 농업생명과학대학 작물생명과학과) ;
  • 정재혁 (농촌진흥청 국립식량과학원)
  • Received : 2020.09.14
  • Accepted : 2020.11.02
  • Published : 2020.12.01
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Abstract

Seed dormancy is an adaptive trait in which seeds do not germinate under unfavorable environmental conditions. Low dormancy seeds are easily germinated under optimal environmental conditions, and these characteristics greatly reduce the yield and quality of crops. In the present study, we compared the pre-harvest sprouting (PHS) rate of two cultivars, Joun and Jopyeong, using the Winkler scale after heading day and temperature of the test. The PHS rate increased as the Winkler scale after heading day increased from 700℃ to 1100℃ and the temperature of the test increased. In all conditions, the PHS rate of Jopyeong was higher than that of Joun. RNA-sequencing was used to analyze the cause of the high PHS rate. We analyzed the biological metabolic processes related to the abscisic acid (ABA) metabolite pathway using the KEGG mapper with selected differentially expressed genes in PHS seeds. We found that the expression of ABA biosynthesis genes (OsNCEDs) was down-regulated and that ABA catabolic genes (OsCYP707As) was up-regulated in PHS seeds. However, the quantitative real-time PCR results showed that Joun had a higher expression of OsNCEDs than that of Jopyeong, but OsCYP707As did not yield a significant result. Joun displayed higher ABA content than that of Jopyeong not only during ripeness time but also during PHS treatment. Taken together, we provided evidence that the ABA content remaining in the seed is important to the PHS rate, which is determined by the expression level of the ABA biosynthesis gene OsNCEDs.

본 연구는 조평과 조운의 벼 출수 후 적산온도와 검정온도에 따른 수발아 발생 정도를 조사하고, RNA-sequencing 방법을 사용하여 수발아 발생 원인을 찾았다. 품종간 수발아성에 관여하는 생리적, 유전학적 요인을 구명하고자 수행하였으며 분석한 결과는 다음과 같다. 1. 출수 후 적산온도가 높아질수록, 검정온도가 높아질수록 수발아 처리시 수발아율이 높았고, 조운벼가 내수발아성이 강하고, 조평벼가 수발아성이 높은것으로 나타났다. 2. 수발아성이 높은 조평벼를 대상으로 한 RNA-sequencing 결과 ABA 생합성에 관여하는 OsNCEDs의 발현이 감소하고, ABA 분해에 관여하는 OsCYP707As의 발현이 증가하였다. 3. 조평과 조운의 OsNCEDs와 OsCYPY707As의 Quantitation Real-Time PCR 결과 조평보다 조운에서 OsNCEDs의 발현이 높게 나타나 수발성과 상관관계를 보였으나, OsCYP707As는 수발아성과 상관관계를 보이지 않았다. 4. 조운벼는 등숙기간중 종실내 ABA함량이 조평보다 높으며 수발아 처리시 남아있는 ABA함량이 높아 내수발아성이 상대적으로 강하게 나타났다.

Keywords

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  1. Seed Dormancy and Pre-Harvest Sprouting in Rice-An Updated Overview vol.22, pp.21, 2020, https://doi.org/10.3390/ijms222111804