• 대한임상검사과학회지
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• 제38권1호
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• pp.72-81
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• 2006

Wee1 is a kinase regulator of the M-phase promoting factor (MPF; a complex of cdc2 and cyclin B1). The present study was undertaken to determine the role(s) of wee1 in the early stages of mouse ovarian follicles. The expression of wee1 and the correlated cell-cycle components, namely cdc2, cyclin B1, and cdc25C, were evaluated by immunohistochemistry. In addition, the expression of Tyr15-phosphorylated cdc2 (cdc2-p) was also examined to determine whether wee1 kinase phosphorylates cdc2 existed. Each component except cdc25C was found cytoplasmic in the oocytes at all stages of follicles, while cdc25C was not detected in primordial follicles. It was found primarily in ovarian somatic cells and to a small extent in granulosa cells of the growing follicles. To further confirm the expression of cell-cycle components in the primordial follicular oocytes, day1 ovaries were enzymatically and mechanically dissociated, then oocytes were isolated from somatic including pre-granulosa cells, and we confirmed that cdc2-p was expressed in oocytes of primordial follicles. From the results of the present study, we concluded wee1, without the counteracting cdc25C, would cause meiotic arrest of oocytes by the inhibitory phosphorylation of cdc2. The expression of all these proteins in the granulosa cells of growing follicles may regulate their mitosis concurrently with the growth of oocytes and follicles.

• 대한기계학회논문집
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• 제18권8호
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• pp.2139-2157
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• 1994

In order to understand the delamination between leadframe and epoxy molding compound in an electronic packaging of surface mounting type, the stress intensity factor, T-stress and J-integral in fracture mechanics are obtained. The effects of geometry, material properties and molding process temperature on the delamination are investigated taking into account the temperature dependence of the material properties, which simulates as more realistic condition. As the crack length increases the J-integral increases, which suggest that the crack propagates if it starts growing from the small size. The effects of the material properties and molding process temperature on stress intensity factor, T-stress is and J-integral are less significant than the chip size for the practical cases considered here. The T-stress is negative in all eases, which is in agreement with observation that interfacial crack is not kinked until the crack approaches the edge of the leadframe.

• 한국안전학회지
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• 제27권5호
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• pp.117-125
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• 2012

In recent years, polymer concrete based on polyester resin have been widely generalized and the research of polymer concrete have been actively pursued by the technical innovations. Polymer concrete is a composite consisting of aggregates and an organic resin binder that hardens by polymerization. Polymer concrete are stronger by a factor of three or more in compression, a factor of four to six in tension and flexural and a factor of two in impact when compared with portland cement concrete. In view of the growing use of polymer concrete, it is important to study the physical characteristics of the material, emphasizing the short term properties as well as long term mechanical behavior. If polymer concrete is to be used in flexural load-bearing application such as in beam, it is imperative to understand the deformation of the material under sustained loading conditions. This study is proposed to empirical and mechanical model of polymer concrete tension creep using long-term experimental results and mathematical development. The test results showed that proposed model has been used successfully to predict creep deformations at a stress level that was 20 percent of the ultimate strength and viscoelastic behavior of recycled-PET polymer concrete is linear of stress level up to 30 percent. It is expected that the present model allows more realistic evaluation of varying stresses in polymer concrete structures with a constant loading.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2008년도 제39회 하계학술대회
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• pp.235-236
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• 2008

Recently, the power load is growing larger and because of the environmental limitation of generation, the expansion of generation facilities are becoming more difficult. For that reason the importance of the demand-side resources come to be higher. One method of the demand-side resource, the DLC Program, has executed, and moreover, the loads which are available to be controlled are increasing. It should be considered of some kinds of power system components such as DLCs, because the fact that using the demand resources will be an important part of the power system. This paper considers the power factor of the load-bus which is shedded in the direct load control program. and then analyze the power system using flow sensitivity and voltage sensitivity. In this paper, we assumed two scenarios through the rank of the load power factor at each bus and to compare and evaluate each case, we used Power World for the simulation.

• 한국자원식물학회지
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• 제34권6호
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• pp.503-509
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• 2021

Marine sources as potential treatment options for various diseases have been a subject of growing interest. However, information on the anti-inflammatory mechanism employed by Undaria pinnatifida (UP) remains limited. The present study was conducted to investigate the mechanisms of UP on the mast cell-mediated inflammatory response. To determine the pharmacological mechanism of UP in inflammatory reaction, we evaluated the effects of UP on interleukin (IL)-6, IL-8 and tumor necrosis factor (TNF)-α production and nuclear factor-κB (NF-κB) and caspase-1 activation in calcium ionophore A23187 plus phorbol 12-myristate 13-acetate-stimulated human mast cells-1 (HMC-1). The results showed that UP suppressed IL-6, IL-8 and TNF-α production in a dose-dependent manner. Moreover, UP significantly attenuated NF-kB/caspase-1 activation in stimulated HMC-1. Collectively, these findings provide experimental evidence that UP may be a useful candidate for the inflammation-related diseases treatment.

• 국제학술발표논문집
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• The 5th International Conference on Construction Engineering and Project Management
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• pp.14-20
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• 2013

Substantial research has been performed on the data standards and exchanges in the AEC/FM industry over the past several years. The growing popularity of BIM technology is based heavily upon a perception that the technology can facilitate the sharing and reuse of information during a project life-cycle. Although many researchers and practitioners are in agreement about the potential applicability and benefit of BIM in construction, it is still unclear why BIM is adopted, and what factors enhance implementation of BIM. Thus, BIM acceptance and use remains a central concern of BIM research and practice. Therefore, we propose the key factors affecting the acceptance of BIM in construction organizations using factor analysis. The key factors for BIM acceptance are identified through a literature review in TAM (Davis 1989) and related theories, and consolidated by interviews and pilot studies with professionals in construction industry. Based on the factors, a questionnaire was designed and sent out to construction organizations such as contractors, architects, and engineers in Korea. Total 148 completed questionnaires were retrieved. Using factor analysis, key factors were grouped into six dimensions. These findings will clarify what the highly prioritized factors are, and can also be used in an assessment tool for the performance of BIM utilization.

• 생명과학회지
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• 제17권10호
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• pp.1321-1329
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• 2007

사슴뿔은 동물세계에서 가장 빨리 성장하는 조직이다. 따라서 성장중인 사슴뿔은 뼈 성장을 촉진하는 인자가 풍부하게 포함된 것으로 생각된다. 이들 성장인자들 중 IGF-1은 뼈를 자라게 하는 조골세포의 대사에 중요한 역할을 한다고 알려져 있어 이를 정제하고자 하였다. IGF-1의 정제는 상대라고 불리는 신선한 사슴뿔을 유안침전, DEAE-Sepharose CL-6B 이온교환수지, CM-Sepharose CL-6B 양이온교환수지, Sephadex G-50의 순차적인 방법으로 할 수 있었다. 각 과정마다 IGF-1의 거동을 HPLC, SDS-PAGE, Dot blot, 그리고 western blot으로 분석하였다. IGF-1의 정량은 ELISA기술로 재조합 인간 IGF-1을 이용하여 계산되었으며, 최종 분별 액은 두 개의 단백질을 보였으나, Western-blot에서 작은 분자량인 12 kDa으로 최종 판명할 수 있었다. 정제된 단백질은 HPLC에서 retention 시간 8분만에 검출되었으며, 총 농도는 2910 ng/ml 이고 중량은 0.291 g 이었다.

• 유통과학연구
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• 제13권6호
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• pp.97-104
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• 2015

Purpose - The distribution of agricultural products is changing due to recent shifts in environmental free trade. Specifically, the competitiveness of domestic agricultural products has weakened as a result of the Korea-China Financial Trade Agreement. Agricultural producers are faced with increasing difficulties and organized production centers are growing in importance daily. To overcome this crisis, agricultural producer organizations are vying for environment-friendly agricultural certifications, Good Agriculture Practices (GAP) and Hazard Analysis and Critical Control Point (HACCP). In particular, as consumer demand for higher safety grows, farmers are increasing their certification rates. Therefore, this certification system is expected to help strengthen the competitiveness of agricultural producer organizations. Research design/data/methodology - Organized production centers are classified by certification. A survey was conducted with 91 organizations using factor analysis and logistic regression analysis for the examination. The factor analysis results are as follows. Raw material procurement, education·specialization, marketing, joint business, organizing ability, business management, effectiveness, certification, and larger organizations were classified as the nine types of factors. These factors affect the organized production centers and are used in the logistic regression analysis. The purpose of such research and analysis is to suggest a direction for future production center policies. Results - The basic statistical results are as follows: analysis of the producer organizations of 91 sites, average number of members per site of 1,624, and average sales of 25,961 million won. Additionally, the average income per farmer is 175 million won, and the pooling system rate is 53.5%. The factor analysis results are as follows. Factor 1 consists of contract cultivation, ongoing shipment, selection subdivision, traceability, and major retailer management. Factor 2 consists of manual cultivation, specialty selection, education program, and R&D. Factor 3 consists of advertising, various dealers, various sales strategies, and a unified sales counter. Factor 4 consists of agricultural materials co-purchase, policy support, co-shipment, and incentives. Factor 5 consists of the co-selection and pooling system. Factor 6 consists of co-branding and operating by the organization's article. Factor 7 consists of the buy-sell ratio and rate of operation of the agriculture promotion center. Factor 8 consists of bargaining power in volume and participation rate of farmer certification. Factor 9 consists of increasing new subscribers. The logistic regression analysis results are as follows. Considering the results by type of certification, the environment-friendly agricultural certification type and the GAP certification type have a (+) influence. GAP and HACCP certification types affecting the education·specialization factor have a (+) influence. Considering the results for each type of certification, the environment-friendly agricultural certification types on the effectiveness factor have (-) influence; the HACCP certification types on the organizing ability and effectiveness factor have a (-) influence. Conclusions - Agricultural producer organizations should develop plans as follows: The organizations need to secure education for agricultural production; increase the pooling system ratio for sustainable organizational development; and, finally, expand the number of agricultural producer organizations.

• 대한기계학회논문집A
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• 제24권6호
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• pp.1565-1574
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• 2000

Surface edge crack subjected to contact stresses is analysed. A punch with corner radii is considered to press the semi-infinite plane. Partial slip problem is solved when a shear force is applied to the punch. Dislocation density function method is used to solve the present mixed mode crack problem. The crack length of positive K1 is examined, which is affected by the ratio of the flat portion to the total width of the punch. Surface traction during one cycle of the shear force is evaluated to simulate the fretting condition. The compliance change of the contact surface is also investigated during the shear cycle. It is found that the crack grows during only a part of the cycle, which may be termed as effective period of crack growing. A design method for restraining the fretting failure is discussed, from which recommendable geometry of the punch is suggested.

• 한국실내디자인학회논문집
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• 제21권2호
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• pp.141-151
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• 2012

Korea is a fast growing aging society. Growing older population causes various social issues and problems in many aspects. Housing issue is one of them and residential modifications for improving safety and independence for older residents are very important factor for Aging in Place. Under those social circumstances, the purpose of this study was to analyze the residential modification demand by the elderly on the application of ubiquitous home services. Respondents were the residents those who were over 55 years old, were living in a singe detached house and were able to use a computer. The major findings of this study were as follows: the results on current situations and demands for residential modifications showed that respondents already made mean of 4.9 home modification items and wanted to make mean of 12.4 items out of 21 residential modification items. The results on demands for the ubiquitous home services was relatively high(mean 3.82). This study can be an important information which is able to improve the quality of life by providing effective residential modifications for older population to help them ultimately safe, convenient, and independent living.