• 한국자원식물학회지
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• 제24권5호
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• pp.636-641
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• 2011

본 연구는 4종의 녹비 작물, hairy vetch, Italian ryegrass, crimson clover, orchard grass 등을 도입하여 연작장해를 일으키는 토마토 시설재배지의 토양개량에 미치는 효과를 구명하였다. 각각의 녹비 작물을 시용한 후, 후 작물로서 토마토를 정식하여 토양의 물리화학성 및 토마토 건물 중 등을 조사하여 녹비작물 재배의 효과 조사하였다. 그 결과 녹비작물 시용에 따라 토양 경도가 감소되었다. Orchard grass와 Italian ryegrass는 토양습도를 증가시키는 요인으로 토양 메트릭스에서 더 많이 무효화 시킬 수 있었다. 녹비작물 시용 2개월 후, Italian ryegrass와 orchard grass 처리구에서 토양 건조 밀도가 감소를 보였으나, 대조구와 녹비구간 통계적 유의성이 인정되지 않았다. 녹비작물로서 Italian ryegrass와 hairy vetch의 시용이 토마토 생육에 영향을 주어 건물중의 증가 및 양호한 생육을 보였다. 또한 orchard grass는 상대적으로 적은 바이오메스인데도 불구하고, 토양 개량에 효과적이었다.

• 농촌의학ㆍ지역보건
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• 제26권2호
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• pp.7-14
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• 2001

증례들은 40대 남자 1명과 여자 1명, 50대 여자 2명이었다. 담배 농사는 모두 10년 이상 종사하였다. 모두 어지러움과 구역을 호소하였으며, 구토, 두통, 설사, 쇠약감 등의 증상이 있었다. 이런 증상은 담배를 집중적으로 수확하는 시기에 발생하였으며, 날씨가 덥거나 땀을 많이 흘리는 경우에 증상이 심했다. 이슬에 젖은 담배잎을 수학하면서 증상이 발현되는 경우도 있었다. 최근 2-3년 동안에 상기 증상들이 1년에 2-3차례 발생하였다. 남자 1명이 고혈압으로 치료하고 있는 것을 제외하면 병력상 특이점은 없었으며, 모두 비흡연자였다. 증례들은 약국과 병원에서 수액요법 등으로 1일 이내에 호전되었다. 우리 나라에서 담배잎농부병의 유병률, 발생률 및 위험요인을 파악하기 위한 역학조사와 병 의원 감시체계를 가동하여야 한다. 담배잎을 수확하다가 어지러움과 구토를 주소로 내원하는 많은 사람들이 실제 담배잎농부병으로 진단되지 못하고 단순한 농약중독과 고온손상에 준하여 치료받는 경우가 많을 것으로 생각한다. 담배를 재배하는 농부는 물론 의료인에 대해서도 담배잎농부병의 예방과 치료에 대한 교육을 실시하여야 한다.

• 식물조직배양학회지
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• 제28권1호
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• pp.53-58
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• 2001

제초제 저항성 오이 (Cucumis sativus L. cv Green angel)를 생산하기 위하여 배발생 현탁배양세포와 binary vector pGA-bar을 지닌 Agrobacterium tumefacians (LBA4404)를 공동배양하였다. 형질전환 벡터의 T-DNA부분에는 kanamycin에 저항성을 나타내는 neomycin phosphotrans ferase (npt II) 유전자와 phosphinothricin (PPT)에 저항성을 나타내는 phosphinothricin acetyltransferase (bar) 유전자를 지니고 있다. 48시간의 공동배양 후 배발생 캘러스는 20mg/L PPT가 함유된 성숙배지에서 배양하였다. 약 200개체의 형질전환 유식물체를 40mg/L PPT가 첨가된 호르몬이 없는 배지에서 생산하였다. 5개의 오이 형질전환 식물체의 염색체에 bar유전자가 도입되어 발현되는 것을 northern blot 분석을 통하여 확인하였다. 형질전환 오이 식물체가 토양에서 성숙되었다. 성숙한 오이 식물체는 PPT가 함유된 상업적 제초제 (Basta)를 일반적인 사용 농도 (3ml/L)처리시에도 저항성을 나타내며 생장하였다.

• 한국환경생태학회지
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• 제22권2호
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• pp.83-97
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• 2008

북한산국립공원의 현존식생 및 녹지자연도와 외래식물 분포실태를 2007년에 조사하였다. 북한산국립공원의 현존식생은 총 8개의 식물군락과 기타 지역으로 구분 되었으며, 자연림은 신갈나무군락, 신갈나무소나무군락, 낙엽활엽수림 등 5개 식물군락으로, 조림지는 잣나무림, 리기다소나무림, 아까시나무림 등 3개 산림유형으로 구분되었다. 북한산국립공원의 녹지자연도에서 가장 넓게 분포한 것은 8등급 지역이 약 92.8%였다. 북한산국립공원안에 식재된 수목류는 총 71과 212종류이었고 이 중 자생종은 37과 67종류(31.6%), 외래종은 58과 145종류(68.4%)이었다. 북한산국립공원의 자연환경 회복, 자생식물의 다양성 유지와 자연경관을 향상시키기 위해서는 외래수종에 대한 관리가 필요하다.

• 한국건축시공학회지
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• 제12권4호
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• pp.401-414
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• 2012

The adoption of Green Frame is expected to provide economic benefits, since construction costs are reduced by the in-situ production of precast concrete column and beam. The cost reduction can ultimately be realized by saving transportation costs and the overhead and profit of PC plants. The cost structure of Green Frame, which is built up using composite precast concrete members, is similar to that of a bearing-wall structure, but the difference in construction process has resulted in some cost differences for a few items. In particular, production and installation is the principal work involved in Green Frame made by precast concrete members, while form and concrete work is the principal work for a bearing-wall structure. As such, the rental time and fee for a tower crane should be compared through time analysis. To verify reliability, this study focused on developed residential projects to estimate the construction costs. Through this analysis, it was found that the costs of Green Frame were 1.57% lower than the costs of bearing-wall structure. The results of this study will help in the development of a management plan for the structural work of Green Frame.

• 아시안잔디학회지
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• 제4권1호
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• pp.5-11
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• 1990

Korean Lawn. Zoysia japonica Steud. is the type of a perennial warm-season turfgrass keeping green leaf color for 5 months from May to September in natural condition in Korea. This Studies were carried out to obtain the effects of N-P-K fertilization, regrowth capacity after mowing and chlorophyll contents on prolongation of the green leaf color period in Zoysia japonica Steud turfgrass areas. The results obtained are summarized as follows ; 1.The effects of nitrogen fertilizer application in Zoysia japonica Steud, turfgrass areas were affected on regrowth ability after mowing green color of leaf canopies and chlorophyll contents, respectively(P<.05) 2.A experimental plots which applied 51 g/m$^2$/10 weeks of N$_3$P$_1$K$_1$fertilizer, there were high significant effects on prolongation of the green leaf color period and the plots of phosphorus and potassium had additive effects. 3.Prolongation of the green leaf color period to the last of October was obviously possible by slow degrees in proportation to increment of the fertilizer. However, it is interpreted that more prolongation of the green period is impossible with N-P-K fertilization and irrigation treatments. unless the intermediate turfgrasses between $C_3$ and $C_4$plants must be found and bred.

• Journal of Forest and Environmental Science
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• 제31권2호
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• pp.136-148
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• 2015

Hyangro-bong is located on the northern side of the civilian access control line and therefore has gone through natural ecological succession without human interference. Currently, it forms a distinctive natural ecosystem in which rare wild animals and plants are various. It is located in the Baekdudaegan mountain system and plays an ecologically important role as a bridge that connects the Geumgang mountain and Seorak mountain. This study investigated the flora of the eastern parts, Forest Genetic Resources Reserve Area, of Hyangro-bong. A total of 363 vascular plants that belonged to 77 families, 206 genera, 310 species, 3 subspecies, and 47 varieties were found in the study area. Rare and endangered plants identified in this study included the critically endangered Swertia wilfordii and endangered Irisuniflora var. caricina. Furthermore, 4 vulnerable species, Hanabusaya asiatica, Codonopsis pilosula, Leontopodium japonicum, and Taxus cuspidata, were found. A total of 7 families and 14 taxa including Aconitum pseudolaeve and Clematis fusca var. coreana were confirmed as endemic plants. Naturalized plants belonging to 4 families, 11 genera, and 13 species were distributed along the Hyangro-bong forest roads.

• The Plant Pathology Journal
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• 제21권2호
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• pp.167-171
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• 2005

We isolated the Cucumber green mottle mosaic virus(CGMMV) particles from watermelon leaves and designated as CGMMV-HY1 as a watermelon isolate and attempted to characterize the pathogenic isolate responsible for such an epidemic in watermelon and also to monitor dominant viral isolates in greenhouse. The watermelon plants infected with CGMMV generally showed mottle mosaic, mosaic, growth stunting, necrosis and deformed fruit. The reactions of indicator plants to CGMMV-HY1 were the local lesions on Nicotiana tabacum cv. White Burley, Nicotiana tabacum cv. Samsun, and Chenopodium amaranticola, and the mosaic symptoms only on Cucumis sativus, but the CGMMV-HY1 did not infect Nicotiana sylvesytis, Datura stramonium, Chenopodium quinoa, and Petunia hybrida. Purified virus particles were rod-shaped and about 300 nm long. The coat protein (CP) of purified CGMMV-HY1 was single band with molecular weight of about 16.5 kDa which was confirmed by western blot analysis probed with monoclonal antibody of CGMMV-HY1. The genomic and subgenomic RNAs of 6.4 kb and 0.75 kb were revealed by the electrophoresis on 1.2% formaldehydedenatured agarose gel. Viral and complementary CGMMV-specific primer sets were designed for spanning the genome using previously reported CGMMV sequences. A 464bp of CP gene of CGMMV-HY1 was amplified by RT-PCR and cloned into PGEM-T easy vector. The nucleotide sequence of CP gene of CGMMV-HY1 shared 98%, 99%, and 100% identities with that of CGMMV strains W, KOM, and KW respectively. Based on these results, we identified CGMMV-HY1 as a CGMMV isolate of watermelon, a member of Tobamovirus.

• Molecules and Cells
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• 제40권10호
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• pp.773-786
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• 2017

The loss of green coloration via chlorophyll (Chl) degradation typically occurs during leaf senescence. To date, many Chl catabolic enzymes have been identified and shown to interact with light harvesting complex II to form a Chl degradation complex in senescing chloroplasts; this complex might metabolically channel phototoxic Chl catabolic intermediates to prevent oxidative damage to cells. The Chl catabolic enzyme 7-hydroxymethyl Chl a reductase (HCAR) converts 7-hydroxymethyl Chl a (7-HMC a) to Chl a. The rice (Oryza sativa) genome contains a single HCAR homolog (OsHCAR), but its exact role remains unknown. Here, we show that an oshcar knockout mutant exhibits persistent green leaves during both dark-induced and natural senescence, and accumulates 7-HMC a and pheophorbide a (Pheo a) in green leaf blades. Interestingly, both rice and Arabidopsis hcar mutants exhibit severe cell death at the vegetative stage; this cell death largely occurs in a light intensity-dependent manner. In addition, 7-HMC a treatment led to the generation of singlet oxygen ($^1O_2$) in Arabidopsis and rice protoplasts in the light. Under herbicide-induced oxidative stress conditions, leaf necrosis was more severe in hcar plants than in wild type, and HCAR-overexpressing plants were more tolerant to reactive oxygen species than wild type. Therefore, in addition to functioning in the conversion of 7-HMC a to Chl a in senescent leaves, HCAR may play a critical role in protecting plants from high light-induced damage by preventing the accumulation of 7-HMC a and Pheo a in developing and mature leaves at the vegetative stage.

• Journal of Crop Science and Biotechnology
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• 제11권3호
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• pp.193-198
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• 2008

To develop a natural re-seeding technology, the optimum soil incorporation time of Chinese milk vetch(CMV) plant residues, seed persistence in soil, biomass production and subsequent rice yield were determined from 2005 to 2007 in rice with CMV green manure in southern Korea. Generally, insufficient seedling stand was regenerated with CMV incorporation to the soil at 20 and 25 days after flowering(DAF), while high regeneration of greater than 400 seedlings per $m^2$ was observed at 35 and 40 DAF. High re-seeding stand of CMV incorporated at 35 DAF or later was related with high seed viability and heavy seed weight. Appreciable number of CMV seeds remained 4 month after burial in soil and a good number of CMV seedlings regenerated from this seed bank at rice harvest time in the fall. Based on the relationships among re-seeding number of CMV plants, seed weight and seed viability, CMV plants should be incorporated into the soil 35 DAF(May 30) or later when CMV seeds were sufficiently matured. The natural re-seeding stand for the 3-year trials was stable ranging from 437 to 700 plants per $m^2$ and the biomass production was sufficient to supply nitrogen for rice growth. The use of re-seeding CMV plant can produce similar rice yield like that of rice without CMV green manure.