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http://dx.doi.org/10.5423/PPJ.2005.21.2.167

Isolation and Characterization of Watermelon Isolate of Cucumber green mottle mosaic virus(CGMMV-HY1) from Watermelon Plants with Severe Mottle Mosaic Symptoms  

Shim, Chang-Ki (Division of Applied Biology & Environmental Sciences, Gyeongsang National University)
Han, Ki-Soo (Division of Applied Biology & Environmental Sciences, Gyeongsang National University)
Lee, Jung-Han (Division of Applied Biology & Environmental Sciences, Gyeongsang National University)
Bae, Dong-Won (Central Laboratory Gyeongsang National University)
Kim, Dong-Kil (Research Institute of Life Science, Gyeongsang National University)
Kim, Hee-Kyu (Research Institute of Life Science, Gyeongsang National University)
Publication Information
The Plant Pathology Journal / v.21, no.2, 2005 , pp. 167-171 More about this Journal
Abstract
We isolated the Cucumber green mottle mosaic virus(CGMMV) particles from watermelon leaves and designated as CGMMV-HY1 as a watermelon isolate and attempted to characterize the pathogenic isolate responsible for such an epidemic in watermelon and also to monitor dominant viral isolates in greenhouse. The watermelon plants infected with CGMMV generally showed mottle mosaic, mosaic, growth stunting, necrosis and deformed fruit. The reactions of indicator plants to CGMMV-HY1 were the local lesions on Nicotiana tabacum cv. White Burley, Nicotiana tabacum cv. Samsun, and Chenopodium amaranticola, and the mosaic symptoms only on Cucumis sativus, but the CGMMV-HY1 did not infect Nicotiana sylvesytis, Datura stramonium, Chenopodium quinoa, and Petunia hybrida. Purified virus particles were rod-shaped and about 300 nm long. The coat protein (CP) of purified CGMMV-HY1 was single band with molecular weight of about 16.5 kDa which was confirmed by western blot analysis probed with monoclonal antibody of CGMMV-HY1. The genomic and subgenomic RNAs of 6.4 kb and 0.75 kb were revealed by the electrophoresis on 1.2% formaldehydedenatured agarose gel. Viral and complementary CGMMV-specific primer sets were designed for spanning the genome using previously reported CGMMV sequences. A 464bp of CP gene of CGMMV-HY1 was amplified by RT-PCR and cloned into PGEM-T easy vector. The nucleotide sequence of CP gene of CGMMV-HY1 shared 98%, 99%, and 100% identities with that of CGMMV strains W, KOM, and KW respectively. Based on these results, we identified CGMMV-HY1 as a CGMMV isolate of watermelon, a member of Tobamovirus.
Keywords
Cucumber green mottle mosaic virus; CGMMVHY1 isolate; coat protein; RT-PCR;
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1 Kim, S. M., Lee, J. M., Yim, K. O., Oh, M. H., Park, J. W. and Kim, K. H. 2003. Nucleotide sequences of two Korean isolates of Cucumber green mottle mosaic virus. Mol. Cell. 16:407-412
2 Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685   DOI   PUBMED   ScienceOn
3 Choi, G. S., Kim, J. H., Chung, B. N., Kim, H. R. and Choi, Y. M. 2001. Simultaneous detection of three Tobamovirus in Cucurbits by rapid immunofilter paper assay. Plant Pathol. J. 17: 106-109
4 Tan, S. H., Nishiguchi, M., Murata, M. and Motoyoshi, F. 2000. The genome structure of Kyuri green mottle mosaic Tobamovirus and its comparison with that of Cucumber green mottle mosaic Tobamovirus. Arch. Virol. 145: 1067-1079   DOI   ScienceOn
5 Yoon, J. Y., Min, B. E., Choi, S. H. and Ryu, K. H. 2001. Completion of nucleotide sequence and generation of highly infections transcripts to cucurbits from full-length cDNA clone of Kyuri green mottle mosaic virus. Arch Virol. 146:2085-2096   DOI   ScienceOn
6 Choi, J. K., Kim, H. J., Yoon, J. Y., Park, S. J., Kim, D. W. and Lee, S. Y. 1998. Detection of virus in fruit and seeds ofvegetables using RT-PCR. Korean J. Plant Pathol. 14:630-635
7 Frabki, R. I. B., Hu, J. and Palukaitis, P. 1986. Taxonomy of cucurbit-infecting obamoviruses as determined by serological and molecular hybridization analyses. Intervirology 26: 156-163   DOI   PUBMED
8 Ryu, K. H., Min, B. E., Choi, G S., Choi, S. H., Kwon, S. B., Noh, G M., Yoon, J. Y, Choi, Y. M., Jang, S. H., Lee, G P., Cho, K. H. and Park, W. M. 2000. Zucchini green mottle mosaic virus is a new Tobamovirus; comparison of its coat protein gene with that of Kyuri green mottle mosaic virus. Arch Virol. 145 :2325-2333   DOI   ScienceOn
9 Hollings, M., Komuro, Y. and Tochihara H. 1975. Cucumber Green Mottle Mosaic Virus. Descriptions of Plant Viruses. No. 154. Kew, Surrey, England; C.M.I./A.A.B
10 Inoue, C., Inoue, S., Asakoku, M. and Mitsuhata, K. 1967. Studies on Cucumber green mottle mosaic virus in Japan. Art. Res. (in Japan) 51: 175-186
11 Choi, J. G., Kang, S. G., Choi, Y. J., Jang, I. S., Suh, S. G and Chung, H. D. 2004. Viral symptoms observed on Korean native Gourd (Lagenaria siceraria) plants and their causal viruses. Kor. J. Hort. Sci. Technol. 22:7-11 (in Korean with English summary)
12 Choi, S. K., Choi, J. K., Ryu, K. H. and Park, W. M. 2002. Generation of infectious cDNA clones of a Korea strain of Tomato espermy virus. Mol. Cells. 13:52-60   PUBMED
13 Lee, S. H., Lee, Y. G, Park, J. W., Choi, H. S., Kim Y T. and Cheon J. U. 2000. Nucleotide sequence of coat protein gene of Kyuri green mottle mosaic virus isolatede from zucchini. Plant Pathol. J. 16:118-124
14 Ugaki, U., Tomiyama, M., Kakutami, T., Hidaka, S., Kiguchi, T., Nagata, R., Sato, T., Motoyoshi, F. and Nishiguchi, M. 1991. The complete nucleotide sequence of Cucumber green mottle mosaic virus (SH strain) genomic RNA. J. Gen. Virol. 71:1487-1495
15 Lee, K. W., Lee, B. C., Park, H. C. and Lee, Y. S. 1990. Occurrence of Cucumber green mottle mosaic virus Disease of Watermelon in Korea. Korean J. Plant Pathol. 6:250-255 (in Korean with English summary)
16 Ryu, K. H. and Park, W. M. 1995. Rapid detection and identification of Odontoglassum rinspot virus by polymerase chain reaction amplification. FEMS Microbiol. Lett. 133:265-269   DOI   ScienceOn