• Journal of Microbiology and Biotechnology
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• v.15 no.1
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• pp.29-34
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• 2005

W/O/W (water-in-oil-in-water) type multiple emulsion was applied to improve the storage stability of an antagonistic microorganism, Burkholderia gladioli. Encapsulation of microorganism into a W/O/W emulsion was conducted by using a two-step emulsification method. W/O/W emulsion was prepared by the incorporation of B. gladioli into rapeseed oil and the addition of polyglycerin polyriconolate (PGPR) and castor oil polyoxyethylene (COG 25) as the primary and secondary emulsifier, respectively. Microcrystalline cellulose was used as an emulsion stabilizer. To evaluate the usefulness of W/O/W emulsion formulation as a microbial pesticide for controlling the bacterial wilt pathogen (Ralstonia solanacearum), the storage stability and antagonistic activity of emulsion formulation were tested in vitro. The storage stability test revealed that the viability of formulated cells in emulsion was higher than that of unformulated cells in culture broth. At $4^{\circ}C$, the viabilities of formulated cells and unformulated cells at the end of 20 weeks decreased to about 2 and 5 log cycles, respectively. At $37^{\circ}C$, the viability of formulated cells decreased to only 2 log cycles at the end of storage. On the other hand, the viable cells in culture broth were not detected after 13 weeks. In activity test, formulated cells in emulsion were more effective in inhibiting the growth of pathogen than unformulated cells in culture broth. Unformulated cells completely lost their antagonistic activity during storage under similar conditions. The W/O/W multiple emulsion formulation was shown to be useful as the novel liquid formulation for biological control.

• The Korean Journal of Food And Nutrition
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• v.13 no.1
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• pp.36-44
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• 2000

Survival of pathogenic bacteris(S. aureus, L. monocytogenes, E. coli and S. typhimurium) in tryptic soy broth containing green tea water extract(GTW), green tea ethanol extract(GTE), potassium sorbate (PS) and sodium benzoate(SB) stored at various pH was evaluated. Tryptic soy broth(TSB) containing 0∼2%(w/v) of green tea extracts and preservatives adjusted to pH 5.5, 6.0, 6.5 and 7.0 was inoculated approximately 105 CFU/ml of pathogenic bacteria and incubated at 35$^{\circ}C$ for 24∼48 hours. Survival of bacteria was determined by viable cell counts of bacterial culture at each pH. Minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC) of green tea extracts and preservatives against pathogenic bacteria were derived from survival curves of each bacteria. Antibacterial activities of green tea extracts increased with increasing pH but those of preservatives decreased with increrasing pH. S. aureus was the most sensitive strain to GTW and GTE but the most resistant to PS and SB. The MICs of green tea extracts to S. aureus were 0.52∼0.98% at pH 5.5∼6.0 and non inhibitory at pH 7.0. S. typhimurium was the most resistant to green tea extracts while the most sensitive to SB. The MICs of green tea extracts to S. typhimurium were 0.46∼1.62% at pH 5.5∼6.0 and 2% of PS was bactericidal at pH 5.5. 1.0∼2.0% of GTE were bactericidal to all strains tested except L. m9oncytogenes at pH 7.0. GTE was most efficient at inactivating pathogenic bacteria, generally followed by GTW, PS and SB.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.3
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• pp.333-337
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• 2015

This experiment was carried out to figure out the growth and yield of waxy corn after cultivating the hairy vetch which was mostly used as a green manure crop. The waxy corn showed the growth and yield efficiency relative to conventional culture after the hairy vetch was returned to as the green manure and the amount of nitrogen fertilizer increased, and the LAI was similar to the conventional culture when less than 18 kg. Length of ear slightly decreased compared to 188 mm of conventional culture when the hairy vetch was used as the green manure and the amount of nitrogen fertilizer was less than 9 kg, but the length of ear increased relative to the conventional culture when the amount of applied nitrogen fertilizer was more than 18 kg. The highest 100-kernel weight was 35.4 g with the hairy vetch and 27 kg of nitrogen fertilizer treatment. The lowest 100-kernel weight was 27.4 g with the hairy vetch and 0 kg of nitrogen fertilizer treatment. Quantity with the hairy vetch and 9 kg of the nitrogen fertilizer treatment was similar the conventional culture. Suger content with the hairy vetch and more than 18 kg of the nitrogen fertilizer treatment was higher than the conventional culture. Thickness of pericarp showed no difference among treatments.

• Korean Journal of Organic Agriculture
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• v.25 no.1
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• pp.113-124
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• 2017

Cotton aphid (Aphis gossypii) and green peach aphid (Myzus persicae) are serious pests damaging various crops including vegetables such as pepper, cucumber, and Chinese cabbage. We conducted a study to control two aphids with secondary metabolite of entomopathogenic fungus Beauveria bassiana. A B. bassiana was already selected as a high virulence isolate against cotton aphid and green peach aphid. The culture filtrate of the isolate showed high pathogenicity against both aphids as 100% mortality against cotton aphid 3 days after treatment and 99% against green peach aphid 5 days after treatment. A different fraction extracts with $CHCl_3$ : MeOH of B. bassiana culture filtrate (30:1, 50:1, 70:1, 90:1, 100:1; v/v) through silica gel column chromatography showed different control effect to aphids. Among them, 50:1 ($CHCl_3$ : MeOH) fraction had highest mortality as 77.3% and 75.4% against A. gossypii and M. persicae, respectively. A mixture of each fraction (1:1) had no synergistic effects because control effect of every mixture was lower than only 50:1 extract; for example, mortality of 50:1 + 70:1 showed $2^{nd}$ highest as 72% of cotton aphid and 70.2% of green peach aphid and other mixtures were lower than these values. In future we will study the identification and mass production of aphicidal compound isolated from 50:1 fraction to develop stable aphid control agent.

• Korean Journal of Plant Tissue Culture
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• v.22 no.1
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• pp.41-46
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• 1995

Leaf mesophyll protoplasts of Dianthus superbus were cultured in MSP1 liquid medium supplemented with 0.5 mg/L BAP, 2.0 mg/L NAA and 9% mannitol. Protoplast-derived colonies were formed after 3 to 4 weeks of culture in the dark at 27$^{\circ}C$. These colonies were kept under continuous illumination (21.5 $\mu$E. m-2 sec-1) for 2 weeks and finally most of the colonies became green microcalli, about 3 mm in diameter. When green microcalli were transferred to MS solidified medium with 2.0 mg/L 2,4-D, they formed embryogenic calli after 4 week of culture. These calli were then transferred onto $N_{6}$ medium containing 0.1mg/L 2,4-D, 0.1 mg/L NAA, 2.0 mg/L kinetin and 2.0 g/L casein hydrolysate and cultured under illumination. After 5 weeks of culture the calli gave rise to multiple shoots of 10 to 15 per callus. Upon transfer onto MS medium containing 2.0 mg/L NAA, they were noted. The regenerates were successfully transplanted into potting soil.

• Journal of the Korean Applied Science and Technology
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• v.29 no.3
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• pp.503-515
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• 2012

${\alpha},{\beta}$-Dichlorosuccinic acid was synthesized through the reaction of maleic anhydride with $Cl_2$(g) and ultra violet(200~300nm) wavelength in the presence of $CCl_4$. The second reaction of N-(${\alpha},{\beta}$-dichloro)succinic acid contained glucosamine derivatives(I) was accomplished by a modification of the general acylation using excess ${\alpha},{\beta}$-dichlorosuccinic anhydride in the presence of 2% acetic acid with methanol as a solvent at elevated temperature($70^{\circ}C$). We considered organic acid derivatives were useful especially of treatment for the cultivating porphyra and skin beauty culture.

• Archives of Pharmacal Research
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• v.27 no.1
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• pp.106-110
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• 2004

The antioxidant activities of Acanthopanax senticosus stems were evaluated in $CCl_4$-intoxicated rats. The n-butanol fraction from the water extract of the stems, when pretreated orally at 200 mg/kg/day for 7 consecutive days in rats, was demonstrated to exhibit significant increases in antioxidant enzyme activities such as hepatic cytosolic superoxide dismutase, catalase and glutathione peroxidase by 30.31, 19.82 and 155%, respectively. The n-butanol fraction whereas showed a significant inhibition of serum GPT activity (65.79% inhibition) elevated with hepatic damage induced by $CCl_4$-intoxication. Eleutheroside B, a lignan component, isolated from the n-butanol fraction was found to cause a moderate free radical scavenging effection DPPH, its scavenging potency as indicated in $IC_{50}$ value, being 58.5$\mu$ M. These results suggested that the stems of A. senticosus possess not only antioxidant but also hepatoprotective activities.

• Journal of Information Technology Applications and Management
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• v.29 no.6
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• pp.123-134
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• 2022

War films are a general term for films that have battlefields as their main background. Although war films as a genre directly deal with combat situations, they also deal with characters or subjects related to war. War films promote patriotism and nationalism, but they also argue against war by highlighting the disastrous war. This study is based on the color theory that the meaning of film color is temporarily and infinitely generated according to the cultural differences, with Eisenstein's creative theory on film color and pathos. I wanted to clarify the pathos effect and the meaning of color green expressed in the Korean war films. In war films, colors are visualized in art forms such as symbols, similes and metaphors. In war films, color green symbolizes life. On the battlefield, the green of nature stands against the catastrophic situation. The green of ecology, which insists on the flow of life, evokes fear in ecological crises such as war, disaster and climate change. The dark green caused by a catastrophe like war warns of the destruction of life. The connotation of color is temporarily and infinitely expands according to the cultural differences. The dark green, which visualizes the battlefield of destruction, is a form and element of pathos that indicates changes in emotions such as sadness, pity, grief and despair. Pathos as an emotional appeal is a leap from the quality to the quality of the means of expression and refers to the departure from Dasein. The green color that dominates the visuals of war films is a symbol of life and functions as a pathos that makes emotional changes take a new leap. A qualitative leap through pathos means all changes that become new.

• Journal of the Korean Society of Food Culture
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• v.3 no.1
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• pp.67-76
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• 1988

With a view to making comparative study and promoting the interchange of food culture between Korea and Japan, a sensory test was given to 60 female college students (30 Koreans and as many Japanese) in order to find out differences in preference of various teas between the two nations. The findings are as follows: 1. The correlation between each specific tea and total evaluation: Significant level of high positive correlation was indicated in case of color, taste, and aftertaste by both Koreans and Japanese. In case of odor, and flavor the Koreans indicated insignificant level of nought, and the Japanese significant level of high negative correlation one. In sweetness and total evaluation the Koreans indicated positive correlation and the Japanese insignificant level of nought. 2. Comparison of preference of various teas by the Koreans and the Japanese 1) Ginseng tea A and Ginseng tea B As to Ginseng tea A and Ginseng tea B the Koreans liked odor best while the Japanese liked flavor best. In general preference the Koreans liked them better than the Japanese, and both groups indicated significant level (p <.001). 2) Black tea A: As to Black tea A the Koreans liked odor best while the Japanese taste, and in general preference the Japanese liked them better than the Koreans and both groups indicated significant level (p <.001). 3) Black tea B. As to Black tea B the Koreans liked odor best while the Japanese color, and in general preference the Japanese liked them better than the Korean and both groups indicated significant level (p <.01). 4) Coffee A. The Koreans liked odor best while the Japanese flavor, and neither of the groups indicated significant level (p <.05). 5) Coffee B. The Koreans liked color best while the Japanese flavor, and neither of the groups indicated significant level (p <.05). 6) Green tea A and Green tea B. As to Green tea A and Green tea B, the Koreans liked odor best while the Japanese taste. In general preference the Japanese liked them better than the Koreans, both indicated significant level (p <.001). 7) Malcha (a kind of traditional Green tea) Both groups liked flavor best, in general preference the Japanese liked it better than the Koreans, and both groups indicated significant level (p <.001).

• Korean Journal of Plant Resources
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• v.18 no.3
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• pp.424-432
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• 2005

Newly introduced two lines of apios (Apios americana Medikus, red-vine and green-vine) were grown in Jeju island, to clarify their growth and production characteristics as well as to develope as a new edible crops in Korea. Both lines bloomed but did not develop to pod and seed. The red-viny line showed the habit of more early growth and maturity compared with green-viny line. Fresh tuber yields per 10a harvested in late November ranged from about 500kg to 800kg as according to the lines and cultural condition. Fresh tuber yield of red-viny line was relatively greater than that of green-viny line, mainly due to their higher tuber number per plant. Among the planting dates(April 1, April 16 and May 1) of seed tubes, highest tuber yield was obtained on May 16 planting. And the stacking cultivation culture was better than non-stacking cultivation in respect of tuber yield and disease avoidance. These results indicate that apios can produce in Jeju island, and in order to extend its cultivation to farmers it will be needed to develope some cultivars with high yields as well as labor-saving cultivation methods.