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http://dx.doi.org/10.11625/KJOA.2017.25.1.113

Aphicidal Activity of Different Fraction Extracts of Culture Filtrate of Beauveria bassiana Isolate against Aphids  

Jeong, Ga-Young (농촌진흥청 국립농업과학원)
Han, Ji-Hee (농촌진흥청 국립농업과학원)
Kim, Jeong-Jun (농촌진흥청 국립농업과학원)
Lee, Sang-Yeob (농촌진흥청 국립농업과학원)
Publication Information
Korean Journal of Organic Agriculture / v.25, no.1, 2017 , pp. 113-124 More about this Journal
Abstract
Cotton aphid (Aphis gossypii) and green peach aphid (Myzus persicae) are serious pests damaging various crops including vegetables such as pepper, cucumber, and Chinese cabbage. We conducted a study to control two aphids with secondary metabolite of entomopathogenic fungus Beauveria bassiana. A B. bassiana was already selected as a high virulence isolate against cotton aphid and green peach aphid. The culture filtrate of the isolate showed high pathogenicity against both aphids as 100% mortality against cotton aphid 3 days after treatment and 99% against green peach aphid 5 days after treatment. A different fraction extracts with $CHCl_3$ : MeOH of B. bassiana culture filtrate (30:1, 50:1, 70:1, 90:1, 100:1; v/v) through silica gel column chromatography showed different control effect to aphids. Among them, 50:1 ($CHCl_3$ : MeOH) fraction had highest mortality as 77.3% and 75.4% against A. gossypii and M. persicae, respectively. A mixture of each fraction (1:1) had no synergistic effects because control effect of every mixture was lower than only 50:1 extract; for example, mortality of 50:1 + 70:1 showed $2^{nd}$ highest as 72% of cotton aphid and 70.2% of green peach aphid and other mixtures were lower than these values. In future we will study the identification and mass production of aphicidal compound isolated from 50:1 fraction to develop stable aphid control agent.
Keywords
aphid; Beauveria bassiana; culture filtrate; entomopathogenic fungi;
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