• Title/Summary/Keyword: Graduate School Program

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A Study on development of oil boom computation program(OBC Program) for mechanical oil clean-up (해상 유출유의 기계적 회수를 위한 소요 오일붐 산정프로그램(OBC Program) 개발에 관한 연구)

  • Moon, Jung-Hwan;Yun, Jong-Hwui;Ha, Min-Jae;Jeon, Da-Woon
    • Journal of Navigation and Port Research
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    • v.35 no.6
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    • pp.495-500
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    • 2011
  • This paper deals with development of an oil boom computation program (OBC Program) for effective response to oil spill. Currently, programs for showing required minimum quantities of oil skimmers and temporary storage tanks have been developed. However, a program for oil boom has not developed, which causes difficulties in oil spill management. The OBC program was developed using LabVIEW 2010 for solving above problems. The OBC program has following strengths: 1. Both experts and non-experts can calculate required quantities of oil booms, 2. The program can save response time, 3. It can reduce damage to properties and marine environment in proportion to reduced response time.

An Empirical Study on Verifying the Estimated Discrimination and Parentage Test Powers of the 13 Traceability Microsatellite Markers for Commercial Pigs Produced by a Three-way Cross (3원교잡 비육돈 집단에 대한 이력추적용 13 Microsatellite Marker의 판별효율 및 혈연관계 추정효율 실증 연구)

  • Lim, Hyun-Tae;Kim, Byeong-Woo;Cho, In-Cheol;Yoo, Chae-Kyoung;Park, Moon-Sung;Park, Hee-Bok;Lee, Jae-Bong;Lee, Jung-Gyu;Jeon, Jin-Tae
    • Journal of Animal Science and Technology
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    • v.53 no.1
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    • pp.29-34
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    • 2011
  • Using the materials collected from nine farms in a three-way cross system to produce commercial pigs produced from F1 sows (Landrace $\times$ Large White) $\times$ Duroc, the power of individual discrimination and parentage of the 13 microsatellite (MS) marker set that has been suggested for individual/brand identification (traceability) was empirically tested. Initially, genotypes of the parental population ($F_1$ sows and Duroc), and commercial pigs were determined and the genotype frequency and polymorphic index were estimated using the Cervus 2.0 program. The probability of identity among genotypes of random individuals, that random half sibs and that of full sib individuals, based on the genotypes from 91 $F_1$ sows and Duroc were expected to be $4.94{\times}10^{-34}$, $8.16{\times}10^{-23}$ and $2.01{\times}10^{-08}$, respectively, using the API-CALC version 1.0 program. When commercial pigs were included, the estimates increased to $3.74{\times}10^{-35}$, $5.48{\times}10^{-25}$ and $2.96{\times}10^{-11}$, respectively. For the empirical verification of the estimated powers of individual discrimination and parentage, the parentage test was performed for 452 commercial pigs using PAPA version 2.0, and individuals with the same genotype were investigated using the Cervus version 2.0 program. Parents for all commercial pigs were successfully estimated and no identical individual was identified in the pedigree. Although the individual discriminating power was not fully verified because of the lack of individuals corresponding with the theoretical power, the 100% efficiency of parentage test was clearly confirmed. Therefore, we believe that the 13 MS marker set in conjunction with management record/information for the pig production kept in a farm/brand should be useful in the pork traceability in a brand unit.

Determination of Total Aflatoxins in Foods by Parallelism of ELISA and LC/MS/MS (ELISA-LC/MS/MS 병행에 의한 식품 중 aflatoxins 분석)

  • Kim, Kyeong-Yeol;Nam, Min-Ji;Nam, Bo-Ram;Ryu, Hee-Jung;Song, Jeong-Eon;Shim, Won-Bo;Lee, Soo-Hyung;Chung, Duck-Hwa
    • Journal of Environmental Health Sciences
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    • v.36 no.1
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    • pp.52-60
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    • 2010
  • High performance liquid chromatography (HPLC) and liquid chromatography mass spectrometry (LC/MS) have been widely used to quantify aflatoxins in food, but these methods are expensive, time-consuming, unsuitable for analysis of the routine screening of large sample numbers and require derivatization and high level techniques to perform. The objective of this study is to detect aflatoxins in a large number of foods by a high efficient analytical system of combined enzyme linked immunosorbent assay (ELISA) for screening and LC/MS/MS for confirmation. The samples spiked individually with aflatoxin $B_1$ (0.5 and 1.0 ng/g) and total aflatoxins (10 ng/g) were analyzed by ELISA and LC/MS/ MS, and the recoveries for ELISA and LC/MS/MS were 71.8~119.2% and 70.8~135.3%, respectively. A total of 378 samples (grains, nuts, soybean and fermented soybean foods, pepper and fermented pepper foods) were purchased from the six major cities in Korea and analyzed by ELISA-LC/MS/MS system. Twenty two (5.8%; peanut: 11, pistachio: 2, walnut: 6, almond: 1, pepper powder: 1, pepper paste: 1) out of 378 samples were screened as aflatoxin B1 positive by ELISA, but, 4 (1.1%; peanut: 2, pistachio:1, pepper powder: 1) out of the 22 samples screened were confirmed as aflatoxins positive at levels of 1.02~52.79 ng/g by LC/MS/MS. ELISA-LC/MS/MS system provides a more rapid, accurate and cost-effective method for the detection of aflatoxins in large number of samples.

Production of Monoclonal Antibody Against Listeria monocytogenes and Its Application to Immunochromatography Strip Test

  • Shim, Won-Bo;Choi, Jin-Gil;Kim, Ji-Young;Yang, Zheng-You;Lee, Kyu-Ho;Kim, Min-Gon;Ha, Sang-Do;Kim, Keun-Sung;Kim, Kwang-Yup;Kim, Cheol-Ho;Ha, Kwang-Soo;Eremin, Sergei A.;Chung, Duck-Hwa
    • Journal of Microbiology and Biotechnology
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    • v.17 no.7
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    • pp.1152-1161
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    • 2007
  • An immunochromatography (ICG) strip test based on a monoclonal antibody for the rapid detection of L. monocytogenes in meat and processed-meat samples was developed in this study. A monoclonal antibody (MAb) specific to L. monocytogenes was produced from cloned hybridoma cells (FKLM-3B12-37) and used to develop an ICG strip test. The antibody showed a stronger binding to L. monocytogenes than other Listeria species, and a weak cross-reaction to S. aureus based on an ELISA. The detection limit of the ICG strip test was $10^5\;cell/ml$. In total, 116 meat and processed-meat samples were collected and analyzed using both the ICG strip test and a PCR. The ICG strip test and PCR indicated L. monocytogenes contamination in 34 and 27 meat samples, respectively. The 7 meat samples not identified as L. monocytogenes positive by the PCR were also tested using an API kit and found to be contaminated by Listeria species. In conclusion, the ICG strip test results agreed well with those obtained using the PCR and API kit. Thus, the developed ICG has potential use as a primary screening tool for L. monocytogenes in various foods and agricultural products, generating results within 20 min without complicated steps.

Development of Direct Competitive Enzyme-Linked Immunosorbent Assay using Monoclonal Antibody (MAb) against Sulfamthazine (SMZ) and Establishment of Application Condition for Milk Sample (설파메타진에 단클론성 항체를 이용한 직접경쟁효소면역분석법의 개발과 우유 시료 적용 조건 확립)

  • Shim, Won-Bo;Mun, Chun-Sun;Kim, Jung-Sook;Choe, Ju-Mi;Kim, Ji-Hun;Park, Seon-Ja;Kang, Sung-Jo;Chung, Duck-Hwa
    • Korean Journal of Food Science and Technology
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    • v.38 no.2
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    • pp.176-182
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    • 2006
  • Sensitive and specific monoclonal antibody (MAb) was produced from hybridoma (1H11-5) obtained by fusion of myeloma cell (V653) and spleen cell isolated from mouse immunized sulfamthazine (SMZ)-HG-KLH. Direct competitive ELISA was developed for rapid detection of SMZ in milk samples using MAb against SMZ with optimized conditions between MAb and SMZ-HG-HRP conjugate, and applicable conditions for analysis of milk samples were established. Detection range of immunoassay was 0.1 to 100 ppb. Recoveries from spiked raw milk and processed milk samples averaged 82.1-120.7 and 82.1-97.1%, respectively.

Economic and Environmental Effect Analysis of Rhodium Recycling System (로듐 재자원화의 경제적 및 환경적 효과 분석)

  • Seong You Lee;Kayoung Shin;Doo Hwan Kim;Yong Woo Hwang;Hong-Yoon Kang;Sung Min Hong;Da-Yeon Kim
    • Resources Recycling
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    • v.32 no.3
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    • pp.45-56
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    • 2023
  • This study analyzed the economic and environmental effect of recycling rhodium used in the non-catalytic field. As an analysis methodology, economic effect analysis applied cost-benefit analysis and resource-saving effect analysis and the environmental effect analysis applied life cycle assessment (LCA). The results show that from an economic point of view, the cost-benefit ratio was 1.28, which was feasible, and the cost reduction was 237,000 won based on 1 g of rhodium recycled and the amount of rhodium recycled was 7.17 billion won in 2025. As for the environmental effect, the greenhouse gas(GHG) emissions were compared between the case of recycling based on rhodium 1 kg and the case of overseas sales. The calculation results show that based on rhodium 1 kg, greenhouse gas emissions were reduced by 99.8%, from 65 kg CO2eq./kg-Rh when recycling to 28,800 kg CO2eq. when sold overseas. The results obtained from this study could suggest that rhodium recycling is necessary in Korea where resources are scarce by analyzing the economic and environmental effect of recycling rhodium used in the non-catalytic field.

The Effects of Combined Exercise Program for Patients with Chronic Neck Pain (만성 경부통 환자를 위한 복합운동프로그램의 효과)

  • Jang, Hyun-Jeong;Kim, Hyun-Hee;Song, Chang-Ho
    • Journal of the Korean Society of Physical Medicine
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    • v.6 no.1
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    • pp.81-92
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    • 2011
  • Purpose : The purpose of this study was to compare combined exercise program group to conventional physiotherapy for patients with chronic neck pain. Methods : Participants were randomly allocated to two groups by experimental(n=17) and control(n=17). Combined exercise program group involved stretching, stability, strengthening and endurance, proprioceptive exercises along with an educational programme. Patient attended for 60min, three times a weeks. Control group was treated as conventional physiotherapy on 40 min, three times a weeks. Outcome variables included pain, disability, range of motion, fatigability at 0 and 6 weeks. Results : Pain, Disability Index, Fatigability significantly decreased(p<.05) and Range of motion improved significantly after active exercise program(p<.05). In conclusion. Combined exercise program improves pain and disability, range of motion, fatigability. Conclusion : These results suggest that combined exercise program is suitable for chronic neck pain.

Simulation Program Verification and Performance Prediction of the Multi-type Heat Pump System

  • Han, Do-Young;Park, Kwan-Jun;Lee, Han-Hong
    • International Journal of Air-Conditioning and Refrigeration
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    • v.9 no.4
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    • pp.47-54
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    • 2001
  • The simulation program which can predict major performances of the multi-type heat pump system was developed. In order to verify the simulation program, several experimental tests were performed under various recommended conditions. The experimental results show in good agreement with simulation results. The verified simulation program was used to analyze the system performance. The capacities and the COP's under the various indoor and outdoor conditions were predicted. Therefore, it may be concluded that the system simulation program developed in this study may be effectively used for the system design and the performance prediction of the multi-type heat pump system.

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THE HST COSMOS PROJECT: CONTRIBUTION FROM THE SUBARU TELESCOPE

  • TANIGUCHI YOSHIAKI;SCOVILLE N. Z.;SANDERS D. B.;MOBASHER B.;AUSSEL H.;CAPAK P.;AJIKI M.;MURAYAMA T.;MIYAZAK S.;KOMIYAMA Y.;SHIOYA Y.;NAGAO T.
    • Journal of The Korean Astronomical Society
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    • v.38 no.2
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    • pp.187-190
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    • 2005
  • The Cosmic Evolution Survey (COSMOS) is a Hubble Space Telescope (HST) treasury project. The COSMOS aims to perform a 2 square degree imaging survey of an equatorial field in I(F814W) band, using the Advanced Camera for Surveys (ACS). Such a wide field survey, combined with ground-based photometric and spectroscopic data, is essential to understand the interplay between large scale structure, evolution and formation of galaxies and dark matter. In 2004, we have obtained high-quality, broad band images of the COSMOS field (B, V, r', i', and z') using Suprime-Cam on the Subaru Telescope, and we have started our new optical multi-band program, COSMOS-21 in 2005. Here, we present a brief summary of the current status of the COSMOS project together with contributions from the Subaru Telescope. Our future Subaru program, COSMOS-21, is also discussed briefly.