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LC-MS/MS를 이용한 반려동물 사료에서 곰팡이독소 동시분석법 유효성 확인 (Validation of simultaneous mycotoxin analysis method in pet food using LC-MS/MS)

  • 최윤화;안우석;김지은;김두환
    • 한국동물위생학회지
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    • 제45권3호
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    • pp.237-242
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    • 2022
  • The simultaneous analysis of mycotoxins using LC-MS/MS, a food official analysis method, was applied with compound feed for pets with high consumer preferences. In this study, the linearity of all calibration curves showed good linearity of 0.99 or more. and both the accuracy (recovery rate) and precision (repeatability) criteria of the concentration range for each mycotoxin in the National Agricultural Products Quality Management Service's Validation and Verification Guidelines were met. And as a result of analyzing FAPAS QCM in the same way, it was assesed that the z-scores of Aflatoxin B1, Ochratoxin A, Zearalenone, and Fumonisin B1, were within ±2 range. This study showed that the application of the food official analysis method to compound feed for pets is suitable.

'질 좋은 청년창업' 창출을 위한 정부의 청년창업육성정책 패러다임 이행방안에 관한 연구 (The Study to The Transformation of Government Youth Entrepreneurship Promotion Policy Paradigm to Create 'Good Quality Youth Startup')

  • 앙영석;양수희;황보윤
    • 벤처창업연구
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    • 제7권3호
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    • pp.167-179
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    • 2012
  • 본 연구는 정부의 청년창업활성화 정책의 현황을 조사하고 이의 성과를 파악하여 문제점을 진단한 후 정부의 청년창업지원정책에 새로운 변화패러다임 방안과 모델을 제시하는 것을 목표 로 하고 있다. 이를 위해 본 연구는 크게 세 가지 연구를 수행하였다. 첫째, 정부의 청년 창업활성화정책을 조사하고 이의 목표 와 현황분석을 통해 지원정책의 구조적 문제점을 진단하는 것이다. 둘째, 선행연구를 통해 정부의 청년창업활성화 정책이 질적 성과를 창출하기 위해 변화해야 하는 업그레이드 방향과 시사점을 도출하였다. 셋째, 청년창업활성화 정책이 양적 중심의 Ver1.0 시대로부터 질적 성과를 강조하는 Ver2.0 창업정책 패러다임으로 이행할 수 있게 하는 청년 창업육성정책 이행모형을 제시하였다. 결론적으로 본 연구에서는 청년창업육성정책이 "열린(open)" 시스템으로 이행되어야 함을 강조했다. 열린 시스템은 청년창업자들과 시장이 협업할 수 있는 매칭기반 구축, 사업을 실전적으로 경험하고 충분히 검토한 후 시장과 연계한 창업을 실행하거나, 창업을 재고해보거나, 아니면 시장그룹에 참여한 선도벤처기업으로의 취업 가능한 경로를 열어주는 것이다. 따라서 이러한 창업육성정책 시스템은 청년들이 현재 처한 상황에서 사업역량을 배가해줌은 물론이고 졸업 후 다양한 선택 대안을 마련해주고 있다는 점이 큰 특징이다.

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식·약공용 농·임산물의 다환방향족탄화수소 오염도 조사 및 위해도 결정 (Contamination Investigation and Risk Characterization on the Polycyclic Aromatic Hydrocarbon of Agricultural Products Used for Food and Medicine)

  • 박영애;고숙경;조성애;정삼주;최은정;홍성초;조석주;정지헌;박주성
    • 생약학회지
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    • 제53권3호
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    • pp.170-180
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    • 2022
  • Polycyclic aromatic hydrocarbons(PAHs) contents were analyzed by measuring benz(a)anthracene(BaA), chrysene(Chr), benzo(b)fluoranthene(BbF) and benzo(a)pyrene(BaP), and the related risk characterization was conducted for 113 samples out of 14 different agricultural products used for food and medicine. Detection rate of PAHs was 90.3% as a whole, and the highest one was 80.5% for BaP. The detection rate of BaP exceeding the maximum permitted concentration of Rehmanniae Radix Preparata and Rehmanniae Radix, 5.0 ㎍/kg was 1.8%, and the detection rates of BaA, Chr and BbF were within the range of 2.7~10.6%. The highest average concentration of BaA was 3.41 ㎍/kg detected from Lycii Fructus, while those of Chr, BbF, BaP and PAH4(sum of detected BaA, Chr, BbF and BaP) were 5.00, 1.79, 2.36, 12.36 ㎍/kg, respectively, detected from Rehmanniae Radix Preparata. As for the risk characterization on PAHs, the overall MOE(Margin of Exposure) values were measured within the range of 105~107, which is unlikely to cause direct health concerns, but the worring values of MOE were measured 6.57×104 for BaP and 6.10×104 for PAH4 from Rehmanniae Radix Preparata, which may require an improvement plan to reduce BaP contents.

산.학.관 협력 어린이집 장학프로그램의 개발 및 적용 (A Study on the Development and Application of the Field-University-Government Cooperation Oriented Supervision Program for Child-care Centers)

  • 남미경
    • 한국보육지원학회지
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    • 제8권6호
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    • pp.193-217
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    • 2012
  • 본 연구는 어린이집 교직원의 전문성 신장을 위한 장학프로그램을 산 학 관 협력으로 계획, 운영하는 프로그램을 개발하고 적용과정을 분석함으로써 어린이집 장학의 활성화 방안을 모색하는데 자료를 제공하고자 하였다. 이를 위해 전문성 평가기준에 따라 선정된 분야별 관련 전문가를 대상으로 한 델파이 조사와 어린이집의 보육교직원을 대상으로 한 적절성 인식조사를 실시하여 프로그램 시안을 개발한 후, 중소도시에 위치한 A어린이집 원장과 교사를 대상으로 장학을 실시하였다. 연구결과, 첫째, 산 학 관 협력 어린이집 장학프로그램 시안은 장학프로그램의 내용 영역 27개 항목과 운영 영역 36개 항목, 총 63개 항목으로 구성되었으며, 적절성 인식 조사결과 장학영역, 운영을 위한 주체별 역할, 운영을 위한 상호협력적 역할에 대해서 모든 항목의 적절성이 평균 3.0이상으로 나타났다. 둘째, 산 학 관 협력 어린이집 장학프로그램의 적용은 3가지 주제를 중심으로 실시하였으며, 실시과정을 통해 참여자들은 산 학 관 협력에 대해 '협력의 이중성', '아쉬움과 한계'를 경험한 것으로 나타났다.

핵심표준기술의 기술이전사업화를 통한 기업성장 및 신산업 창출 사례연구 - 이니텍(주) ETRI 협력사례를 중심으로 - (A Case study of firm growth and new market creation through commercialization of standard technology - Collaboration between ETRI and Initech -)

  • 손익수;고영희
    • 지식경영연구
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    • 제14권5호
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    • pp.15-34
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    • 2013
  • In a turbulent environment, it is important for a firm to obtain competitiveness through acquiring new technologies and new knowledge. Many studies has shown that R&D has positive effects on innovation and performance. However, the limitation of resources and research personnel for SMEs leads to the needs of collaboration between government R&D and SMEs. The quantitative results of government R&D comes in the form of patents or publication of papers while the qualitative results comes in the form of commercialization (revenue increase or cost reduction) of the SMEs acquiring the technology through technology transfer. Korean government invested 16.9 Trillion Won in 2013 and achieved remarkable quantitative results but the qualitative results are still yet to be improved. The technology transfer rate in 2011 was 26.0% while the success rate of commercialization of the transferred technology is only 23.6%. Therefore it is meaningful to study the success factors of the technology commercialization in Korea. In this study, a case of technology commercialization between ETRI and Initech is studied. Initech was a venture firm which started in 1997 with 2 employees and after transfer of technology from ETRI in 1999, it has grown to a firm of 238 employees. In this study, Jolly's commercialization model was used to analyze the success factors of the commercialization. It was shown that four main factors that leads to the success are (1) mobilizing interest and endorsement through customer oriented R&D by ETRI, (2) mobilizing resources for demonstration through systematic technology commercialization process by ETRI, (3) mobilizing market constitutents through government standardization that leads to the reliability of the participants, and (4) mobilizing complementary assets for delivery by management strategies of Initech.

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생활폐기물처리시설 민영화 정책에 대한 지방자치단체 의사 분석 (An Analysis of Local Government's Intentions for Privatization Policy of Waste Treatment Facilities in Korea)

  • 구자건;강미연;서용칠;선종근;윤보라;김병훈
    • 환경영향평가
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    • 제18권5호
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    • pp.257-270
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    • 2009
  • The central government in Korea has pursued a policy to privatize municipal solid waste treatment facilities in order to enhance the effectiveness of public financial management in recent years. To understand the intentions of local governments on this policy, the survey with questionnaires on 37 out of 256 local governments was made in October, 2007. The results were analyzed and compared in 6 classified policy areas. The results showed that local government had enough financial status to operate the facilities with proper services and had the opinion with positive management in finance with increasing rate of prospective views. Among the facilities operated by local governments, the satisfaction levels for landfill(46.2%) and incineration(40.0%) were relatively higher. However those for recycling and food waste resource facilities were not satisfied with showing lower rate of satisfaction. The priority of privatization in consideration among them were following in order of food waste resource plants(76.2%), incinerators(63.6%), and recycling facilities(53.9%) and the most considerable reason to privatize them was due to resolve financial problems(59.7%). They thought and expected that the privatization would provide cost reduction effects in local governments and also be easier to achieve the environmental goals. To enhance such privatization, expanding more incentives by central government would be essential as shown in the investigation of this survey.

불임시술의 합병증에 관한 역학적 연구 (An Epidemiological Study on the Complications caused by the Sterilization Program)

  • 홍명선
    • 지역사회간호학회지
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    • 제7권1호
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    • pp.138-153
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    • 1996
  • Intending to offer basic information for a prospective health services in Korea, this study is to investigate the complication caused by sterilization in goverment family planning program from 1962 to 1995. The results are as follows: 1. Total number of sterilization performed during the period from 1962 to 1995 were 1.367,772 cases of male sterilization and 2,889,635 cases of female sterilization. 2. Incidence of the complication caused by sterilization operation from 1980 to 1995 were 1,883(0.20%) out of 925,801 cases in vasectomies and 15,866(0.70%) out of 2,256,020 cases in tubal sterilizations. 3. Major complications in vasectomy were epididymities of 658 cases (34.9%), vas recanalization of 326 cases(17.3%), hematoma of 266 cases(14.1%), scrotal abscess of 184 cases(9.8%), sperm granuloma of 76 cases(4.0%),and other of 373 cases(19.8%). On the other hand, in tubal sterilization, ectopic pregnancy was the most significant complication of 15,078 cases (95.0%) among 15,866 total complications, followed by pelvic inflammatory diseases of 155 cases(0.9%), peritonities of 96 cases(0.6%), ovarian & tubal bleeding of 31 cases(0.2%), intestinal perforation of 16 cases (0.1%), uterine bleeding of 14 cases(0.1%), uterine cervix laceration of 1 case (0.1%), and other of 271 cases(1.7%), while 161 pregnancies(0.1%) were terminated and 43 cases(0.3%) with normal delivery. 4. The occurrence rate of the complication for each period showed that most of the complication cases by vasectomy occurred in a year after the operation -the cases were 1,256 (66.7%). 254 cases(13.5%) occurred between the next year and the 2nd year, 138 cases (7.3%) between the 2nd year and the 3rd year, 73 cases(3.9%) between the 3rd year and the 4th year, 52 cases(2.8%) between the 4th year and the 5th year, 31 cases(1.6%) between the 5th year and the 6th year, 79 cases(4.2%) over the 6th year. Tubal sterilization indicated that the occurred complication cases in a year were 2,175 cases(13.7%), 2,113 cases(13.3%) occurred between the next year and the 2nd year, 2,082 cases(13.1%) between the 2nd year and the 3rd year, 2,049 cases (12. 9%) between the 3rd year and the 4th year, 1,819 cases(11.5%) between the 4th year and the 5th year, 621 cases(10.2%) between the 5th year and the 6th year, 4,007 cases(25.3%) over the 6th year. 5. For the cost of complication treatment, total \7,928,229,000 were paid as medical expenditure in which \609,438,000 for vasectomy and \7,318,791,000 for tubal sterilization. Accordingly per capita expenses were \345,000 for vasectomy and \467,000 for tubal sterilization. As the proportion of government sterilization program was decreased after 1988, that of private sterilization program would be increased. So it is recommended to set a guideline for the private sterilization program and to continue government sterilization program for the lower class.

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산증류-HPLC법과 모니어윌리암스변법을 이용한 절임류중의 이산화황 함량 분석 (Determination of Sulfur Dioxide in Pickles by Acid Distillation-HPLC Method and Monnier Williams Modified Method)

  • 정소영;김일영;김성단;장미라;장민수;한기영
    • 한국식품과학회지
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    • 제35권6호
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    • pp.1028-1032
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    • 2003
  • 절임류에 함유된 이산화황함량의 정확한 분석을 위해 산 증류후 HPLC-UV detector를 이용한 방법과 Monnier-Williams변법으로 실험해보았다. 산증류-HPLC-UV법은 절임류중의 아황산염을 산증류 시킨 후 발생하는 이산화황을 1% triethanolamine용액에 흡수시켜 이를 UV 240nm에서 정량하는 것으로서, 고정상은 음이온 교환컬럼을, 이동상으로는 1.8mM $Na_2Co_3$와 1.7mM $NaHCO_3$용액을 사용하였다. 이 방법으로 단무지에 아황산염을 첨가하여 실험 한 결과 $84.0{\sim}91.7%$의 회수율을 얻었다. 시중에 유통되는 48종의 절임류에 대해 모니어윌리암스변법과 산증류-HPLC-UV법을 이용하여 이산화황 함량을 조사해본 결과, 산증류-HPLC-UV법에 비해 모니어월리암스법에서 휘발성 산으로 인해 높은 농도를 나타내었다. 산증류-HPLC-UV법으로 분석한 절임류의 이산화황 농도는 불검출${\sim}173.05ppm$이었고, 기준인 30ppm을 초과하는 제품은 3건이었다.

A comparison of ATR-FTIR and Raman spectroscopy for the non-destructive examination of terpenoids in medicinal plants essential oils

  • Rahul Joshi;Sushma Kholiya;Himanshu Pandey;Ritu Joshi;Omia Emmanuel;Ameeta Tewari;Taehyun Kim;Byoung-Kwan Cho
    • 농업과학연구
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    • 제50권4호
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    • pp.675-696
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    • 2023
  • Terpenoids, also referred to as terpenes, are a large family of naturally occurring chemical compounds present in the essential oils extracted from medicinal plants. In this study, a nondestructive methodology was created by combining ATR-FT-IR (attenuated total reflectance-Fourier transform infrared), and Raman spectroscopy for the terpenoids assessment in medicinal plants essential oils from ten different geographical locations. Partial least squares regression (PLSR) and support vector regression (SVR) were used as machine learning methodologies. However, a deep learning based model called as one-dimensional convolutional neural network (1D CNN) were also developed for models comparison. With a correlation coefficient (R2) of 0.999 and a lowest RMSEP (root mean squared error of prediction) of 0.006% for the prediction datasets, the SVR model created for FT-IR spectral data outperformed both the PLSR and 1 D CNN models. On the other hand, for the classification of essential oils derived from plants collected from various geographical regions, the created SVM (support vector machine) classification model for Raman spectroscopic data obtained an overall classification accuracy of 0.997% which was superior than the FT-IR (0.986%) data. Based on the results we propose that FT-IR spectroscopy, when coupled with the SVR model, has a significant potential for the non-destructive identification of terpenoids in essential oils compared with destructive chemical analysis methods.

Wild Mushrooms: A Potential Source of Nutritional and Antioxidant Attributes with Acceptable Toxicity

  • Sharif, Sumaira;Shahid, Muhammad;Mushtaq, Muhammad;Akram, Sumia;Rashid, Ayoub
    • Preventive Nutrition and Food Science
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    • 제22권2호
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    • pp.124-130
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    • 2017
  • This paper describes in detail proximate composition, nutritional profile, phytochemical constituents, antioxidant activities, antimicrobial potential, and antihemolytic activity (towards human erythrocytes) of various fractions of wild Ganoderma lucidum. Proximate analysis established that wild G. lucidum comprises about $87.02{\pm}5.45%$ of moisture, and the remaining part is a rich source of proteins ($8.59{\pm}0.37%$), crude fiber ($54.21{\pm}1.2%$), and carbohydrate (35.16%) with smaller fat content (3.33 %). Similarly, phytochemical screening revealed the presence of flavonoids ($217.51{\pm}0.30mg/g$), ascorbic acid ($116{\pm}7.32mg/g$), phenolics ($360.72{\pm}34.07mg/g$), ${\beta}$-carotenes ($0.42{\pm}0.04{\mu}g/g$), and lycopene ($0.05{\pm}0.00{\mu}g/g$). Extracts of wild G. lucidum in various solvents provided first line protection against Escherichia coli and Pasteurella multocida in the order of ethyl acetate> ethanol> methanol> n-hexane> water. Furthermore, aqueous and methanolic extracts of wild G. lucidum were found to be safe towards human erythrocytes. Overall, wild mushroom (G. lucidum) was found to be a good source of dietary supplements, antimicrobial and antioxidant agents in the pursuance of its commercial utilization in food and pharmaceutical industries.