• Applied Microscopy
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• v.39 no.3
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• pp.253-260
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• 2009

GM-CSF is a multipotent growth factor, which also plays an important role during the process of wound healing. rrhGM-CSF was specifically produced from rice cell culture in our laboratory (Hanson Biotech Co., Ltd, Daejeon). The rrhGMCSF contains more oligosaccharide side chains than any other types of GM-CSF. This work was taken to evaluate the influence on wound healing of rrhGM-CSF in male golden hamsters. Full thickness skin defects of 9 mm in diameter were made in the back of hamsters, and 100 ${\mu}L$ ointment containing rrhGM-CSF 50 ${\mu}g/mL$ was applied. Control groups were given ointment without rrhGM-CSF. The wound sizes were relatively reduced and skin was well regenerated in the experimental group compared with the control group. Structurally, reepithelialization and architecture of the skin following injury were well accomplished in the experimental group. And also, positive reaction of PCNA of the skin following injury was more prominent in rrhGM-CSF containing ointment treatment group. Since this type of GM-CSF has highly glycosylated side chains, the effectiveness might be retain longer and stable, regarding acceleration of wound healing in the animal model. The present study has important implications for further development of the therapeutic manipulation of wound healing using rrhGM-CSF.

• Korean Journal of Weed Science
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• v.32 no.1
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• pp.10-16
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• 2012

This study was carried out to investigate the agronomic traits, comparison of weed characteristics and possibility of gene flow in 'vitamin A enforced GM rice' and the donor plant, 'Nagdong'. The GM rice was not significantly different agronomic traits compared to the donor plant, Nagdong. Weed population changes were investigated in the cultivation of the GM rice and the donor plant, Nagdong. Dominant weed species and their dry matter did not show the difference between GM rice and the donor plant, Nagdong in macro-GM crop field. Dominant weed species with the GM rice and the donor plant, Nagdong were Monochoria vaginalis, followed by Eleocharis kuroguwai, Echinochloa crus-galli and Lindernia procumbens. The detection of gene from the GM rice was done using PCR, gene flow can't be detected by weed species. Results of this study on the agronomic traits, weed characteristics and possibility of gene flow has elucidated that GM rice might not be different from the donor plant, Nagdong.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.44 no.5
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• pp.560-564
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• 2011

Two feeding experiments were conducted to investigate the effects of dietary inclusion of genetically modified (GM) soybean and corn on the growth performance, feed utilization and body composition of juvenile abalone Haliotis discus hannai. Four isonitrogenous (31% crude protein) and isolipidic (6% crude lipid) diets (designated as nGM-soya, GM-soya, nGM-corn and GM-corn) were formulated to contain 20% non-GM (nGM) and GM soya and corn. Fifty juvenile abalone (initial body weight, 2.0 g) were distributed in each 50 L tank in a flow-through system. Each experimental diet was fed to duplicate groups of abalone to satiation once a day for 10 weeks. No effects of GM feedstuffs on survival were observed. Dietary inclusion of GM feedstuffs did not affect either growth performance or feed utilization of abalone. Body composition was not altered by the inclusion of GM feedstuffs. These results indicate that dietary inclusion of GM soybean and corn could have no effect on the growth performance and body composition of juvenile abalone. Further studies to investigate the effects of GM feedstuffs on transgenic fragment residues in ambient environments and in animals are necessary for the safe use of such ingredients in aquaculture.

• Korean Journal of Environmental Agriculture
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• v.31 no.1
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• pp.52-59
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• 2012

BACKGROUND: Cultivation of genetically modified(GM) crops rapidly has increased in the global agricultural area. Among those, herbicide resistant GM crops are reported to have occupied 89.3 million hectares in 2010. However, cultivation of GM crops in the field evoked the concern of the possibility of gene transfer from transgenic plant into soil microorganisms. In our present study, we have assessed the effects of herbicide-resistant GM Chinese cabbage on the surrounding soil microbial community. METHODS AND RESULTS: The effects of a herbicide-resistant genetically modified (GM) Chinese cabbage on the soil microbial community in its field of growth were assessed using a conventional culture technique and also culture-independent molecular methods. Three replicate field plots were planted with a single GM and four non-GM Chinese cabbages (these included a non-GM counterpart). The soils around these plants were compared using colony counting, denaturing gradient gel electrophoresis and a species diversity index assessment during the growing periods. The bacterial, fungal and actinomycetes population densities of the GM Chinese cabbage soils were found to be within the range of those of the non-GM Chinese cabbage soils. The DGGE banding patterns of the GM and non-GM soils were also similar, suggesting that the bacterial community structures were stable within a given month and were unaffected by the presence of a GM plant. The similarities of the bacterial species diversity indices were consistent with this finding. CONCLUSION: These results indicate that soil microbial communities are unaffected by the cultivation of herbicide-resistant GM Chinese cabbage within the experimental time frame.

• Journal of the Korean Society of International Agriculture
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• v.30 no.4
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• pp.347-356
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• 2018

Genetically modified (GM) crops have been increased continuously over the world and concerns about the potential risks of GM crops have also been increasing. Even though GM crops have not been cultivated commercially in Korea, it should be necessary to develop the safety assesment technology for GM crops. In this study, we investigated the influence of heading date difference on gene flow from GM to non-GM rice. In the experimental plot design, The PAC GM rice was placed in the center as a pollen donor and non-GM rice were placed in eight directions as pollen receivers. Five pollen receiver rice cultivars were Unkawng, Daebo, Saegyejinmi, Nakdong-byeo, and Ilmi which had different flowering times. A total of 266,436, 300,237, 305,223, 273,373, and 290,759 seeds were collected from Unkawng, Daebo, Saegyejinmi, Nakdong, and Ilmi, respectively, which were planted around PAC GM rice. The GM${\times}$non-GM hybrids were detected by repeated spraying of herbicide and PAT immunostrip assay. Finally, the hybrids were confirmed by PCR analysis using PAC gene specific primer. The hybrids were found in Nakdong-byeo which had the same heading date with PAC GM rice. The hybridization rate was 0.0007% at Nakdong-byeo plot. All of GM${\times}$non-GM hybrids were located within 2 m distance from the PAC GM rice zone. The physiological elements including rice heading date were found to be important factors to determine GM?rice out crossing rate with GM rice. Consideration should be taken into for many factors like the physiological elements of field heading date of rice cultivars to set up the safety management guideline for prevention of GM rice gene flow.

• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.2
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• pp.174-180
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• 2016

In this study, the effects of fermented goat milk (F-GM) on immunological activity and physical strength were examined. Splenocytes obtained from mice administered with F-GM showed increased responsinveness to mitogens, concanavalin-A (ConA), and lipopolysaccharide (LPS). Treatment with F-GM also significantly augmented production of interleukin (IL)-2 and interferon (IFN)-${\gamma}$, but not IL-4 or IL-10 from ConA-stimulated splenocytes. The activity of F-GM administration to enhance production of IL-2 and IFN-${\gamma}$ was confirmed based on mRNA expression of these cytokines by reverse transcription polymerase chain reaction. After immunization with keyhole limpet hemocyanin (KLH, 20 mg/mouse), mice administered F-GM showed significantly higher antibody titers against KLH than those of phosphate-buffered saline-treated mice, and showed the highest titer 5 weeks after KLH immunization. Analysis for determining isotypes of antibodies revealed that administration of F-GM elicited KLH-specific antibody titers of IgG1, IgG2a, and IgM. In a delayed type hypersensitivity (DTH) test carried out 7 weeks after the primary immunization, F-GM-treated mice showed a higher DTH reaction than the control mice. Furthermore, the swimming test found that administration of F-GM significantly increased swimming time. These results suggest that administration of F-GM enhances not only immune responses against antigens but also physical strength.

• Journal of The Korean Society of Grassland and Forage Science
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• v.35 no.4
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• pp.303-308
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• 2015

Cultivating genetically modified (GM) crops is believed to be a practical solution to meet the increasing food demand, but GM crops are not legal in Korea mainly due to food safety issues. Even though the general public might not be ready to consume GM food, GM crops are imported and consumed as food and feed. To analyze farmers's willingness to grow GM crops for feed, a survey was conducted among crop farmers and 640 valid responses were collected by mail. In the questionnaire, the farmers were asked to select either 'yes' or 'no' if they were willing or not willing to cultivate GM rice and GM grass, respectively, under the given hypothetical income increase rate (i.e., 10%, 20%, 30%, 40%, 50%, 60%, or 70%). Logit regression was used to estimate the two dichotomous choices by explanatory variables including hypothetical income increase rate. The results show that farmers are willing to cultivate GM rice and grass when their income is expected to increase by 47% and 43%, respectively.

• Korean journal of applied entomology
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• v.56 no.4
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• pp.331-338
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• 2017

In the present study, we investigated the effects of two herbicide-resistant genetically modified rice (GM rice) varieties, Milyang 204 and Iksan 483, recently developed in Korea on non-target insects and a spider. No difference in host preferences of the English grain aphid Sitobion avenae and the brown planthopper Nilaparvata lugens were observed between GM rice and non-GM rice. Wolf spider Pirata subpiraticus, feeding on N. lugens reared on GM rice or non-GM rice, revealed no significant difference in body weight. P. subpiraticus, fed with N. lugens reared on Milyang 204, showed survival rates similar to that in P. subpiraticus fed with N. lugens reared on non-GM rice. However, P. subpiraticus feeding on N. lugens reared on Iksan 483 demonstrated significantly lower survival rates than that in P. subpiraticus feeding on N. lugens reared on Milyang 204 or non-GM rice. In addition, when larvae of the western honeybee Apis mellifera were supplied with Iksan 483 pollen, a significantly longer pupal period occurred, as compared with that of A. mellifera supplied with pollen of Milyang 204 or non-GM rice. As GM rice has negative effects on P. subpiraticus, which is an important predator in agricultural ecosystems, and on A. mellifera, which plays important roles in pollination and honey production, additional studies on risk assessment of GM rice should be conducted before releasing newly developed herbicide-resistant GM rice to the agricultural environment.

• Proceedings of the Botanical Society of Korea Conference
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• 1985.08b
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• pp.23-33
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• 1985

Plant cell culture is emerging to express bioactive foreign proteins because it has several advantages in that it is safe, economical, genetically stable and eukaryotic expression system comparing with other expression systems. However several limitations such as slow growth rate, low expression level and lack of well established down stream process need to be answered. As a preliminary approach to produce the immunologically interested molecules through the plant cell culture, we tested if granulocyte-macrophage colony stimulating factors (GM-CSFs) from both murine (mGM-CSF) and human (hGM-CSF) are produced as a biologically active form through plant cell culture. The murine and human GM-CSF genes were cloned into the plant expression vector, pBI121, and Ti-plasmid mediated transformation of tobacco leaves was conducted using Agrobacterium tumefaciens harboring both recombinant GM-CSF (rGM-CSF) genes. Cell suspension culture was established from the leaf-derived calli of transgenic tobacco plant. Northern blot analysis indicated the expression of the introduced mGM-CSF gene in both transgenic plant and cell suspension cultures. In addition, the biological activities of both murine and human GM-CSF from plant cell culture were confirmed by measuring the proliferation of the GM-CSF dependent FDC-PI and TF-1 cells, respectively.

• Journal of Plant Biotechnology
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• v.36 no.3
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• pp.261-267
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• 2009

The coexistence policy of GM and non-GM crops is still on the debates in EU since “the recommendation on guidelines for the development of national strategies and best practices to ensure the coexistence of GM crops with conventional and organic farming” has been reported in 2003. The major issues are maximum tolerance level of GMO admixture and minimum isolation distances of GM fields with others including conventional, organic and seeds production. Majority of member states in EU proposed that the tolerance level of GMO admixture must be more strictly controlled, in particular in the fields for organic crops and seeds production. To this end, it was proposed that minimum isolation distances to segregate GM crops from fields with organic crops and seeds production need to be further extended than those of conventional crops since cross pollination with other crops adjacent GM fields is known as the most prevalent source for GMO contamination. In these circumstances, it is strongly suggested that the current legislations need to be revised including the minimum isolation distances of fields for each species before field cultivation for a commercial GM crop is approved for the first time in South Korea.