• 한국식품위생안전성학회지
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• 제38권3호
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• pp.176-183
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• 2023

홍삼의 일반적인 가공법인 물과 주정 혼합용매로 추출 여과하여 농축액으로 제조 시 증숙 등의 가열처리에 의해 가수분해되거나 중합반응이 일어나게 되어 현재 기능성이 밝혀진 30여 가지 주요 진세노사이드 외 극미량인 200여종의 진세노사이드가 검출되고 있어서, 오히려 홍삼 본래의 기능성 규명과 효능 구현을 복잡하게 만들고, 추출 횟수를 거듭할수록 떫은맛이 강해지는 등 건강기능식품으로서 부정적인 영향을 배제하기 위하여 저온(40℃ 미만)에서 1차만 추출한 홍삼농축액을 제조한 결과 수율은 고형분 66-77% 정도에서 26-30%를 나타내어 기존의 연구 결과인 40.47±0.47% 보다 현저히 적은 수율을 나타내었고, 동결건조 분말의 진세노사이드 함량분석시험을 진행한 결과 시료 18종 전체에서 진세노사이드 34종이 검출되었고, 특히 홍삼 지표성분 3종인 Rb₁, Rg₁, Rg₃ 합계는 42.48 mg/g으로 시판 홍삼제품의 환산량 9.0 mg/g과 8.7 mg/g 보다 4-5배 많았다. 본 시료는 진한 홍삼의 향취와 함께 면역력 증진 등 6가지 기능발현에 9-25%의 소량 복용으로 1일 복용량이 유의성 있게 작아서 복용의 편의성과 함께 제품의 보관, 유통에 장점이 있는 것으로 판단된다.

• Journal of Ginseng Research
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• 제20권1호
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• pp.54-59
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• 1996

Red Ginseng extracts sol'n was sterilized at 85f for 20 mins and/or stored at 4$0^{\circ}C$ for 6 months and centrifuged for 20 mins at 8,500xg in order to investigate the changes in chemical components of supernatants and the properties of precipitates. Contents of crude saponin and ginsenoside-$Rb_1$, -$Rg_1$, -Re were partially decreased during heating and storage. Starch contents were decreased from 26.81% in red ginseng extracts to 17.50-8.81% in supernatants, whereas free sugar contents were increased from 15.50% to 20.29~21.35% by heating and storage. The contents of protein and minerals in supernatants were decreased, but acidic polysaccharides and polyphenol compounds were not changed. pH values of supernatants and precipitates were decreased. The absorbances of brown color precursor and brown pigment in precipitates, detected at 285 nm and 440 nm were remarkably increased. The Overa11 data suggest that precipitates in red ginseng extracts sol'n formed during steilization and storage are provably the brown pigments resulting from Maillard reaction of amino compounds with reducing sugar which could be released from starch and protein matrix and $Cu^+$, $Ca^{2+}$ and $Fe^{3+}$ ions are implicated with the reaction incorporated.

• Journal of Ginseng Research
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• 제38권3호
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• pp.161-166
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• 2014

Ginseng is widely used for its promising healing and restorative properties as well as for its possible tonic effect in traditional medicine. Nowadays, many studies focus on purified individual ginsenoside, an important constituent in ginseng, and study its specific mechanism of action instead of whole-plant extracts on cardiovascular diseases (CVDs). Of the various ginsenosides, purified ginsenosides such as Rb1, Rg1, Rg3, Rh1, Re, and Rd are the most frequently studied. Although there are many reports on the molecular mechanisms and medical applications of ginsenosides in the treatment of CVDs, many concerns exist in their application. This review discusses current works on the countless pharmacological functions and the potential benefits of ginseng in the area of CVDs. Results: Both in vitro and in vivo results indicate that ginseng has potentially positive effects on heart disease through its various properties including antioxidation, reduced platelet adhesion, vasomotor regulation, improving lipid profiles, and influencing various ion channels. To date, approximately 40 ginsenosides have been identified, and each has a different mechanism of action owing to the differences in chemical structure. This review aims to present comprehensive information on the traditional uses, phytochemistry, and pharmacology of ginseng, especially in the control of hypertension and cardiovascular function. In addition, the review also provides an insight into the opportunities for future research and development on the biological activities of ginseng.

• 한국식품과학회지
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• 제43권4호
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• pp.413-418
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• 2011

대장균군은 유통형, 맞춤형 및 건강기능형 홍삼음료 모두에서 음성이었다. 일반세균은 유통형 홍삼음료 및 건강기능형 홍삼음료에서는 검출되지 않았으나, 맞춤형 홍삼음료에서는 58건 중 13건(22.4%)이 검출되었다. 총 100건의 제품 중 5건의 제품에서 안식향산이 검출되었다. 함량은 19.2-543.0 mg/kg이며 이 중 유통형 홍삼음료에서 검출된 1건의 제품(19.2 mg/kg)과 건강기능형 홍삼음료 1건(31.4 mg/kg)의 함량은 원료에서 유래된 것으로 판단되었다. 유통형 홍삼음료의 납 평균 함량은 62.3 ${\mu}g/kg$, 맞춤형 홍삼음료는 74.6 ${\mu}g/kg$, 건강기능형 홍삼음료는 28.8 ${\mu}g/kg$으로 나타났다. 카드뮴의 평균 함량은 유통형 1.15 ${\mu}g/kg$, 맞춤형 1.62 ${\mu}g/kg$, 건강기능형 홍삼음료는 4.18 ${\mu}g/kg$으로 조사되었다. 수은의 평균 함량은 0.10-0.18 ${\mu}g/kg$으로 조사되었다. 홍삼 건강기능식품의 지표성분인 Rg1과 Rb1함량은 유통형 및 맞춤형 홍삼음료가 0.1-23.4 mg/90 mL으로 함유되어 있었다. 하루에 3회 섭취한다고 가정하고 건강기능식품의 일일 섭취량 3-80 mg/day와 비교해보면, 이번에 조사된 37건의 유통형 홍삼음료 중에서 22건(59%), 맞춤형 홍삼음료 58건에서는 26건(45%)이 건강기능식품에 해당하는 지표성분(Rg1과 Rb1)을 함유하고 있는 것을 알 수 있었다. 그러나 홍삼음료들 간에 서로 홍삼성분의 함량차이가 크고 건강기능식품 섭취기준(3-80 mg/day) 양에 미치지 못한 47건 중 1회 분량 90 mL 기준 홍삼성분이 0.2 mg 이하로 함유되어 있는 제품도 20 건이나 되는 것으로 나타났다.

• Journal of Ginseng Research
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• 제44권2호
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• pp.291-299
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• 2020

Background: Ginseng (G) and Ligustrum lucidum Ait (LLA) are core traditional Chinese medicines in treating myelosuppression formula. The present study was designed to profile effect of G and LLA herb pair (G-LLA) on myelosuppressed mice. Methods: The mice myelosuppression model was established by intraperitoneal (i.p.) injection of cyclophosphamide (Cy). Hematopoietic function of bone marrow was measured by hemopoietic progenitor cell culture and peripheral blood count, and serum hemopoietic factors were tested by enzyme-linked immunosorbent assay. Bone marrow cell cycle was performed by flow cytometry. HPLC was used to measure 20 potential chemical components related to myelosuppression, including ginsenoside Rg₁, Re, Rb₁, Rc, Rb₂, Rb₃, Rd, Rk₃, Rh₄, 20 (S)-Rg₃, 20 (R)-Rg₃, Rk₁, Rg₅, salidroside, and so on. Results: G, LLA, and G-LLA improved the amount of peripheral blood cells and bone marrow cells of myelosuppressed mice (P < 0.01). They significantly increased the colony quantity of colony-forming unit-granulocyte macrophage, burst-forming unit-erythroid, colony-forming unit-erythroid, and colony-forming unit-megakaryocyte and amount of G₂/M and S phase cells (P < 0.01). They also significantly decreased the amount of hematopoiesis-related cytokines (P < 0.01). The content of chemical components in G-LLA changed, and the change of rare saponin was the most obvious. Conclusion: These results show that G-LLA herb pair might produce synergistic or complementary compatibility effects on bone marrow suppression after chemotherapy. It suggests that the substance basis of G-LLA for treating bone marrow suppression may be effective chemical components.

• 분석과학
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• 제30권3호
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• pp.146-153
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• 2017

In this study, we isolated ginseng crude saponin (GCS) from Korean red ginseng (KRG) and determined the ginsenoside content in it to investigate the physiological and pathological effects of GCS on acute pulmonary inflammation induced by intratracheal instillation of cigarette smoke condensates (CSC) and lipopolysaccharide (LPS) solution in BALB/c mice. GCS was orally administered at doses of 10 mg/kg and 25 mg/kg for 3 weeks. The recovery rate of GCS from KRG was 6.5 % and total ginsenosides from GCS was 1.13 %, and the content of Rb1 was the highest among them. Total inflammatory cells in the lung homogenates and bronchoalveolar lavage fluid (BALF) increased following intratracheal administration of CSC and LPS. However, GCS administration impaired this increase. Furthermore, it inhibited the increase in leukocytes in the blood, considerably decreased neutrophils in BALF, and declined infiltration of inflammatory cells and deposition of collagen in the tracheal and alveolar tissue. In this study, GCS was found to have a protective effect against acute pulmonary inflammation and it may be beneficial in preventing various respiratory diseases.

• Journal of Applied Biological Chemistry
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• 제64권3호
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• pp.253-261
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• 2021

본 연구에서는 식물공장을 통해 생산된 새싹인삼을 크기에 따라 분류하고 이를 지상부와 지하부로 나누어 진세노사이드 함량과 항산화 활성을 비교하였다. 지상부의 경우 총 phenolic 함량은 중간 크기인 M에서 5.16 GAE mg/g로 가장 높았으며 가장 큰 크기인 L에서 2.23 GAE mg/g으로 가장 낮은 함량을 보였다. 지하부 역시 M 크기에서 가장 높은 함량을 보였으나, 큰 차이를 보이지 않았다. 한편, 총 flavonoid 함량 역시 지상부(5.16 RE mg/g) 와 지하부(1.28 RE mg/g) 모두 M 크기에서 높은 함량을 보였다. 지상부의 주요 진세노사이드는 Re (20.33-24.15 mg/g) > Rd (11.36-27.42 mg/g) > Rg1 (4.48-5.54 mg/g) 순 있었고 지하부는 Rb1 (5.09-8.61 mg/g) > Re (4.48-5.54 mg/g) > Rc (3.11-4.11 mg/g) 순 있었다. M 크기의 경우 Re와 Rd는 각각 지상부에서 24.15 mg/g과 27.42 mg/g 및 지하부에서 5.20 mg/g과 1.43 mg으로 약 4배와 19배 높은 함량을 보였다. 지상부에는 F3 및 Rh1이 검출되었으나, 지하부에서는 검출되지 않았다. DPPH (74.95%)와 ABTS (94.47%), hydroxyl (70.39%) 라디칼 소거 활성 및 FRAP (2.169) 활성은 다른 크기들보다 M 크기에서 가장 높은 활성을 보였다.

• Molecules and Cells
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• 제21권1호
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• pp.52-62
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• 2006

In previous reports we demonstrated that ginsenosides, active ingredients of Panax ginseng, affect some subsets of voltage-dependent $Ca^{2+}$ channels in neuronal cells expressed in Xenopus laevis oocytes. However, the major component(s) of ginseng that affect cloned $Ca^{2+}$ channel subtypes such as ${\alpha}_{1C}$(L)-, ${\alpha}_{1B}$(N)-, ${\alpha}_{1A}$(P/Q)-, ${\alpha}_{1E}$(R)- and ${\alpha}_{1G}$(T) have not been identified. Here, we used the two-microelectrode voltage clamp technique to characterize the effects of ginsenosides and ginsenoside metabolites on $Ba^{2+}$ currents ($I_{Ba}$) in Xenopus oocytes expressing five different $Ca^{2+}$ channel subtypes. Exposure to ginseng total saponins (GTS) induced voltage-dependent, dose-dependent and reversible inhibition of the five channel subtypes, with particularly strong inhibition of the ${\alpha}_{1G}$-type. Of the various ginsenosides, $Rb_1$, Rc, Re, Rf, $Rg_1$, $Rg_3$, and $Rh_2$, ginsenoside $Rg_3$ also inhibited all five channel subtypes and ginsenoside $Rh_2$ had most effect on the ${\alpha}_{1C}$- and ${\alpha}_{1E}$-type $Ca^{2+}$ channels. Compound K (CK), a protopanaxadiol ginsenoside metabolite, strongly inhibited only the ${\alpha}_{1G}$-type of $Ca^{2+}$ channel, whereas M4, a protopanaxatriol ginsenoside metabolite, had almost no effect on any of the channels. $Rg_3$, $Rh_2$, and CK shifted the steady-state activation curves but not the inactivation curves in the depolarizing direction in the ${\alpha}_{1B}$- and ${\alpha}_{1A}$-types. These results reveal that $Rg_3$, $Rh_2$ and CK are the major inhibitors of $Ca^{2+}$ channels in Panax ginseng, and that they show some $Ca^{2+}$ channel selectivity.

• Journal of Dairy Science and Biotechnology
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• 제32권1호
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• pp.47-53
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• 2014

본 연구는 김치에서 분리한 L. acidophilus를 이용하여 홍삼 농축액의 진세노사이드 변화 및 폴리페놀 변화량을 확인하였고, 발효유제품 중 신선치즈를 선정하여 홍삼 발효물의 기능성 소재로의 사용 가능성을 확인하였다. 홍삼농축액3% 처리구에 L. acidophilus 유산균주를 $1.0{\times}10^8CFU/mL$로 첨가하여 $40^{\circ}C$에서 24시간 발효한 경우 유산균수는 발효 0시간째 $3.5{\times}10^8CFU/mL$에서 발효 16시간째 $3.8{\times}10^8CFU/mL$로 증가하였다가 그 후 감소하여 발효 24시간째에는 $2.2{\times}10^8CFU/mL$로 측정되었다. Ginsenoside 전환 양상은 고분자 물질로는 Rb1, Rb2, Rc, Rd, Re, Rf, Rg1이 검출되었고, 저분자 물질로는 Rg3(20S), Rg3(20R), Rh2(20S), Rh1(20R), Rh2(20S), Rh2(20R), F1, Compound K, Protopanaxadiol(20S), Protopanaxadiol(20R)이 검출되었다. 고분자 물질이 감소함에 따라 저분자 물질인 Rg3(20S) 및 Rg3(20R), protopanaxadiol(20R), F1, Compound K 등이 증가하였다. 홍삼 발효물의 총 페놀 화합물의 변화량은 에틸아세테이트 분획물 및 16% ACN 분획물에서 발효시간이 증가할수록 폴리페놀 함량이 증가하였다. 홍삼 발효물을 첨가하여 제조한 신선치즈의 저장 중 품질변화를 분석하였으며, 홍삼 발효물의 첨가농도가 높아질수록 pH는 저장기간 동안 감소하였고, 산도 및 유산균수는 증가하였다. 관능검사 결과 홍삼 발효물 1% 처리구가 대조구와 유사한 평가를 얻었으며, 이때의 사포닌 함량은 14.8 mg%, 총 페놀함량은 3.7 mg%이었다. 따라서 향후 동물모델을 통한 추가적인 효능검증이 이뤄진다면 홍삼 발효물을 이용한 기능성 강화 고부가가치 신선치즈 제조가 가능할 것으로 보인다.

• 생약학회지
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• 제16권3호
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• pp.171-174
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• 1985

Panax ginseng root has been widely used as an important drug for thousands years in China, Korea and Japan. The main effective components of ginseng have been believed to be saponins. However, ginseng cultivation is very difficult and needs many years for growth. It has already been shown that Panax ginseng callus produces a considerable amount of the same kinds of saponins as in intact plants. Various culture conditions were examined for increased production of ginseng saponins by cell culture. The saponin contents and the growth rates in two cell lines of ginseng callus were compared in static and suspension cultures, rotary and reciprocal shaking cultures. It was shown that the growth rate in rotary shaking cultures of D5-B2K-B2K callus was the highest and ginseng saponin production was most effective in reciprocal cultures of D5-B2K-B2K callus. The saponin content per fresh weight of the culture was 1.03 times higher than that of the fresh ginseng root.