Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Comparison of ginsenoside contents and antioxidant activity according to the size of ginseng sprout has produced in a plant factory

식물공장에서 생산된 새싹인삼의 크기에 따른 진세노사이드 함량 및 항산화 활성 비교

  • Hwang, Seung Ha (Department of Food Science, Gyeongsang National University) ;
  • Kim, Su Cheol (Department of Food Science, Gyeongsang National University) ;
  • Seong, Jin A (Department of Food Science, Gyeongsang National University) ;
  • Lee, Hee Yul (Department of Food Science, Gyeongsang National University) ;
  • Cho, Du Yong (Department of Food Science, Gyeongsang National University) ;
  • Kim, Min Ju (Department of Food Science, Gyeongsang National University) ;
  • Jung, Jea Gack (Department of Food Science, Gyeongsang National University) ;
  • Jeong, Eun Hye (Department of Food Science, Gyeongsang National University) ;
  • Son, Ki-Ho (Department of Horticultural Science, Gyeongsang National University) ;
  • Cho, Kye Man (Department of Food Science, Gyeongsang National University)
  • Received : 2021.05.14
  • Accepted : 2021.07.29
  • Published : 2021.09.30
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Abstract

In this study, the ginseng sprout has produced through smart farm was classified according to its size and divided into above-ground (AG) and below-ground (BG) parts to compare ginsenoside contents and antioxidant activity. In the case of the AG part, the total phenolic contents were the highest at 5.16 mg/g in medium (M) size and the lowest at 2.23 mg/g in largest (L) size. The BG part also showed the highest content in the M size, but there was no significant difference. Also, the total flavonoid contents were also high in the M size in both the AG (5.16 mg/g) and BG (1.28 mg/g) parts. The major ginsenosides in the AG part were Re (20.33-24.15 mg/g) > Rd (11.36-27.42 mg/g) > Rg1 (4.48-5.54 mg/g) and the main ginsenosides in the BG part were Rb1 (5.09-8.61 mg/g) > Re (4.48-5.54 mg/g) > Rc (3.11-4.11 mg/g) in orders. In the case of M size, Re and Rd were approximately 4- and 19-folds higher at 24.15 mg/g and at 27.42 mg/g in the AG part and 5.20 mg/g and 1.43 mg in the BG part, respectively. In addition, F3 and Rh1 were detected in the AG part, but not in the BG part. 2,2-diphenyl-1-picrylhydrazyl (74.95%), 2,4,6-azino-bis (3-ethylbenzothiazoline-6-sulphnoic acid) diammonium salt (94.47%), and hydroxyl (70.39%) radical scavenging activities and FRAP (2.169) assay were the highest in M size than other sizes.

본 연구에서는 식물공장을 통해 생산된 새싹인삼을 크기에 따라 분류하고 이를 지상부와 지하부로 나누어 진세노사이드 함량과 항산화 활성을 비교하였다. 지상부의 경우 총 phenolic 함량은 중간 크기인 M에서 5.16 GAE mg/g로 가장 높았으며 가장 큰 크기인 L에서 2.23 GAE mg/g으로 가장 낮은 함량을 보였다. 지하부 역시 M 크기에서 가장 높은 함량을 보였으나, 큰 차이를 보이지 않았다. 한편, 총 flavonoid 함량 역시 지상부(5.16 RE mg/g) 와 지하부(1.28 RE mg/g) 모두 M 크기에서 높은 함량을 보였다. 지상부의 주요 진세노사이드는 Re (20.33-24.15 mg/g) > Rd (11.36-27.42 mg/g) > Rg1 (4.48-5.54 mg/g) 순 있었고 지하부는 Rb1 (5.09-8.61 mg/g) > Re (4.48-5.54 mg/g) > Rc (3.11-4.11 mg/g) 순 있었다. M 크기의 경우 Re와 Rd는 각각 지상부에서 24.15 mg/g과 27.42 mg/g 및 지하부에서 5.20 mg/g과 1.43 mg으로 약 4배와 19배 높은 함량을 보였다. 지상부에는 F3 및 Rh1이 검출되었으나, 지하부에서는 검출되지 않았다. DPPH (74.95%)와 ABTS (94.47%), hydroxyl (70.39%) 라디칼 소거 활성 및 FRAP (2.169) 활성은 다른 크기들보다 M 크기에서 가장 높은 활성을 보였다.

Keywords

Acknowledgement

본 연구는 2021년 농촌진흥청 농축산물 수출확대 장애요인해소기술개발(과제번호: PJ015655)과 2018년 산학연협력 기술개발(과제번호: S266050) 연구비 지원에 의하여 연구되었습니다.

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