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http://dx.doi.org/10.5806/AST.2017.30.3.146

Ginsenosides analysis in the crude saponin fraction extracted from Korean red ginseng, and its efficacious analysis against acute pulmonary inflammation in mice  

Lee, Seung Min (Department of Industrial Plant Science & Technology, Chungbuk National University)
Lim, Heung Bin (Department of Industrial Plant Science & Technology, Chungbuk National University)
Publication Information
Analytical Science and Technology / v.30, no.3, 2017 , pp. 146-153 More about this Journal
Abstract
In this study, we isolated ginseng crude saponin (GCS) from Korean red ginseng (KRG) and determined the ginsenoside content in it to investigate the physiological and pathological effects of GCS on acute pulmonary inflammation induced by intratracheal instillation of cigarette smoke condensates (CSC) and lipopolysaccharide (LPS) solution in BALB/c mice. GCS was orally administered at doses of 10 mg/kg and 25 mg/kg for 3 weeks. The recovery rate of GCS from KRG was 6.5 % and total ginsenosides from GCS was 1.13 %, and the content of Rb1 was the highest among them. Total inflammatory cells in the lung homogenates and bronchoalveolar lavage fluid (BALF) increased following intratracheal administration of CSC and LPS. However, GCS administration impaired this increase. Furthermore, it inhibited the increase in leukocytes in the blood, considerably decreased neutrophils in BALF, and declined infiltration of inflammatory cells and deposition of collagen in the tracheal and alveolar tissue. In this study, GCS was found to have a protective effect against acute pulmonary inflammation and it may be beneficial in preventing various respiratory diseases.
Keywords
ginsenosides analysis; crude ginseng saponin; pulmonary inflammation; pathophysiological analysis;
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1 T. K. Yun, J. Kor. Med. Sci., 16(Suppl), S3 (2001).   DOI
2 M. Hong, Y. H. Lee, S. Kim, C. S. Bang, J. H. Yoon, G. H. Baik, D. J. Kim, and M. J. Kim. J. Ginseng Res., 40(3), 203-210 (2016).   DOI
3 S. I. Chung, M. Y. Kang, and S. C. Lee, Prev. Nutr. Food Sci., 21(1), 24-30 (2016).   DOI
4 J. G. Park, Y. J. Son, A. Aravinthan, J. H. Kim, and J. Y. Cho, J. Ginseng Res., 40(4), 431-436 (2016).   DOI
5 H. U. Lee, E. A. Bae, M. J. Han, N. J. Kim, and D. H. Kim, Liver Int., 25(5), 1069-1073 (2005).   DOI
6 D. H. Kim, J. H. Chung, J. S. Yoon, Y. M. Ha, S. Bae, E. K. Lee, and H. Y. Chung, J. Ginseng Res., 37(1), 54-60 (2013).   DOI
7 J. H. Kim and J. S. Choi, Physiol. Res., 65(6), 1031-1037 (2016).
8 J. Bousquet, J. Kiley, E. D. Bateman, G. Viegi, A. A. Cruz, N. Khaltaev, and G. W. Canonica, Eur. Respir. J., 36(5), 995-1001 (2010).   DOI
9 H. Oh and Y. E Lee, Osong Public. Health Res. Perspect., 7(6), 385-393 (2016).   DOI
10 J. Lee. J. H. Lee, J. A. Kim, and C. K. Rhee, Int. J. Chron. Obstruct Pulmon Dis., 12, 27-33 (2016).   DOI
11 J. Vestbo, S. S. Hurd, A. G. Aqusti, P. W. Jones, C. Vogelmeier, A. Anzueto, P. J. Barnes, L. M. Fabbri, F. J. Martinez, M. Nishimura, R. A. Stockley, D. D. Sin, and R. Rodriquez-Roisin, Am. J. Respir Crit. Care Med., 187(4), 347-365 (2013).   DOI
12 M. Maskey-Warzechowska, P. Neiman-Gryz, K. Osinka, P. Lis, K. Malesa, K. Gorska, and R. Krenke, Adv. Exp. Med. Biol., 944, 73-78 (2017).
13 M. Kaur and D. Singh, J. Pharmacol. Exp. Ther., 347(11), 173-180 (2013).   DOI
14 K. H. Lee, C. H. Lee, J. Jeong, A. H. Jang, and C. G. Yoo, J. Biol. Chem., 290(47), 28438-28445 (2015).   DOI
15 A. Bhowmik, T. A. Seemungal, R. J. Sapaford, and J. A. Wedzicha, JA, Thorax., 55(2), 114-120 (2000).   DOI
16 J. C. Hogg, F. Chu, S. Utokaparch, R. Woods, W. . Elliott, L. Buzatu, and P. D. Pare, New. Eng. J. Med., 350(26), 2645-2653 (2004).   DOI
17 D. Raghavan, T. Bartter, and M. Joshi, Curr. Opin. Pulm. Med., 22(2), 106-112 (2016).   DOI
18 O. Kornmann, R. Dahl, S. Centanni, A. Dogra, R. Owen, C. Lassen, and B. Kramer, Eur. Respir. J., 37(2), 273-279 (2011).   DOI
19 S. Apikoglo-Rabus, G. Yesilyaprak, and F. V. Izzettin, Respir. Med., 120, 109-115 (2016).   DOI
20 H. W. Ryu, S. U. Lee, S. Lee, H. H. Song, T. H. Son, Y. U. Kim, H. J. Yuk, H. Ro, C. K. Lee, S. T. Hong, and S. R. Oh, Cytokine, 91, 57-64 (2016).
21 H. G. Kwak and H. B. Lim, Chin. J. Nat. Med., 12(9), 641-647 (2014).   DOI
22 S. K. Kim, Y. S. Kwak, S. W. Kim, S. Y. Hwang, Y. S. Ko, and C. M. You, J. Ginseng Res., 22(3), 155-160 (1998).
23 S. Shibata, O. Tanaka, T. Ando, M. Sado, S. Tsushima, and T. Ohsawa, Chem. Pharm. Bull.(Tokyo), 14(6), 595-600 (1966).   DOI
24 L. Cooling, S. Hoffmann, D. Webb, M. Meade, C. Yamada, R. Davenport, and G. Yanik, J. Clin. Apheresis, 32(1), 35-48 (2017).   DOI
25 Y. Chen, Z. Zhao, H. Chen, E. Brand, T. Yi, M. Qin, and Z. Liang, Int. Immunopharmacol., 28(2), 1097-1101 (2017).   DOI
26 H. Lee, K. H. Jung, H. Lee, S. Park, W. Choi, and H. Bae, Int. Immunopharmacol., 28(2), 1097-1101 (2015).   DOI
27 K. Kuwano, J. Araya, H. Hara, S. Minagawa, N. Takasaka, S. Ito, K. Kobayashi, and K. Nakayama, Respir. Investig., 54(6), 397-406 (2016).   DOI
28 D. D. Sin and S. P. Man, Circulation, 107(11), 1514-1519 (2003).   DOI
29 N. Takabatake, H. Nakamura, S. Abe, S. Inoue, T. Hino, H. Saito, and H. Tomoike, Am. J. Respir. Crit. Care Med., 161(4), 1179-1184 (2000).   DOI
30 I. S. Shin, K. S. Ahn, N. R. Shin, H. J. Lee, H. W. Ryu, J. W. Kim, K. Y. Sohn, H. J. Kim, Y. H. Han, and S. R. Oh, Int. Immunopharmacol., 30, 62-68 (2016).   DOI
31 X. Wang, T. Sakuma, E. Asafu-adjaye, and G. K. Shiu, Anal. Chem., 71(8), 1579-1584 (1999).   DOI
32 P. J. Barnes, S. D. Shapiro, and R. A. Pauwels, Europ. Respir. J., 22(4), 672-688 (2003).   DOI
33 J. L. Wright and A. Churg, Histopathology, 49(1), 1-9 (2006).   DOI
34 T. Min, M. Bodas, S. Mazur, and N. Vij, J. Molec. Med., 86(6), 577-593 (2011).