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http://dx.doi.org/10.1016/j.jgr.2019.01.001

Compatibility effects of ginseng and Ligustrum lucidum Ait herb pair on hematopoietic recovery in mice with cyclophosphamide-induced myelosuppression and its material basis  

Han, Jiahong (College of Chinese Medicinal Materials, Jilin Agricultural University)
Dai, Min (College of Chinese Medicinal Materials, Jilin Agricultural University)
Zhao, Yan (College of Chinese Medicinal Materials, Jilin Agricultural University)
Cai, Enbo (College of Chinese Medicinal Materials, Jilin Agricultural University)
Zhang, Lianxue (College of Chinese Medicinal Materials, Jilin Agricultural University)
Jia, Xiaohuan (College of Chinese Medicinal Materials, Jilin Agricultural University)
Sun, Nian (College of Chinese Medicinal Materials, Jilin Agricultural University)
Fei, Xuan (College of Chinese Medicinal Materials, Jilin Agricultural University)
Shu, Hui (College of Chinese Medicinal Materials, Jilin Agricultural University)
Publication Information
Journal of Ginseng Research / v.44, no.2, 2020 , pp. 291-299 More about this Journal
Abstract
Background: Ginseng (G) and Ligustrum lucidum Ait (LLA) are core traditional Chinese medicines in treating myelosuppression formula. The present study was designed to profile effect of G and LLA herb pair (G-LLA) on myelosuppressed mice. Methods: The mice myelosuppression model was established by intraperitoneal (i.p.) injection of cyclophosphamide (Cy). Hematopoietic function of bone marrow was measured by hemopoietic progenitor cell culture and peripheral blood count, and serum hemopoietic factors were tested by enzyme-linked immunosorbent assay. Bone marrow cell cycle was performed by flow cytometry. HPLC was used to measure 20 potential chemical components related to myelosuppression, including ginsenoside Rg1, Re, Rb1, Rc, Rb2, Rb3, Rd, Rk3, Rh4, 20 (S)-Rg3, 20 (R)-Rg3, Rk1, Rg5, salidroside, and so on. Results: G, LLA, and G-LLA improved the amount of peripheral blood cells and bone marrow cells of myelosuppressed mice (P < 0.01). They significantly increased the colony quantity of colony-forming unit-granulocyte macrophage, burst-forming unit-erythroid, colony-forming unit-erythroid, and colony-forming unit-megakaryocyte and amount of G2/M and S phase cells (P < 0.01). They also significantly decreased the amount of hematopoiesis-related cytokines (P < 0.01). The content of chemical components in G-LLA changed, and the change of rare saponin was the most obvious. Conclusion: These results show that G-LLA herb pair might produce synergistic or complementary compatibility effects on bone marrow suppression after chemotherapy. It suggests that the substance basis of G-LLA for treating bone marrow suppression may be effective chemical components.
Keywords
Chemotherapy; Ginseng; Ginsenge-Ligustrum lucidum Ait herb pair; Material basis; Myelosuppression;
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