• Title/Summary/Keyword: Ginkgo leaves

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Seasonl Variations of Biflavone Content from Ginkgo biloba Leaves (은행잎 중 Biflavone의 계절별 함량 변화에 관한 연구)

  • Chang, Soo-Kyung;Youm, Jeong-Rok;Kang, Sam- Sik
    • Korean Journal of Pharmacognosy
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    • v.24 no.1
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    • pp.54-57
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    • 1993
  • The seasonal variations of five biflavones from Ginkgo biloba leaves from May to November were investigated by a reversed phase HPLC method. The total amount of biflavones was increased with time to reach its maximum in yellow autumnal leaves. Each biflavone showed a similar tendency. It increased rapidly about 3.1-fold from May to June and thereafter gradually increased about 2.5-fold. The ratio of each biflavone content to the total amount of biflavones was in the order of as follows: isoginkgetin>sciadopitysin>bilobetin>ginkgetin>amentoflavone.

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A Study on the Correlation between Lead in Airborne Suspended Particulates and Leaves of Roadside Trees in Urban Area (대기부유분진중 Pb와 가로수잎중 Pb와의 상관성에 관한 연구)

  • 고현규;김희강;현용범
    • Journal of Korean Society for Atmospheric Environment
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    • v.2 no.3
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    • pp.11-18
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    • 1986
  • In order to evaluate the state of the environmental pollution by vehicle exhaust in Seoul area the correlation between lead in air and leaves of roadside trees has been investigated during August in 1985. The dust in the atmosphere was collected by high volume air sampler to measure the concentration of lead. On the other hand, lead as a pollution indicator was extracted from the leaves of roadside trees (Ginkgo biloba, Salix pseudo/lasiogyne, platanus occidentalis) by conventional method and their concentrations were determined. The following results were obtained : 1. Lead concentrations in the leaves of roadside trees varied with trees, for example, the average concentrations of lead in each of the leaves of Ginkgo biloba, Salix pseudo/lasiogyne and Platanus occidentalis were 20.66 ppm, 9.37 ppm, and 10.58 ppm, respectively. 2. The dust sampled along heavily traveled highways showed that lead content tended to increase with traffic volume. 3. The correlation coefficients between lead correlation in air and leaves of Gingo biloba, Salix pseudo/lasiogyne, and Platanus orientalis were 0.80, 0.85, and 0.87, respectively.

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The Effect of Plant Extracts on In-vitro Ruminal Fermentation, Methanogenesis and Methane-related Microbes in the Rumen

  • Kim, E.T.;Min, K.S.;Kim, C.H.;Moon, Y.H.;Kim, S.C.;Lee, Sung-Sill
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.4
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    • pp.517-522
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    • 2013
  • The effect on methanogens attached to the surface of rumen ciliate protozoa by the addition of plant extracts (pine needles and ginkgo leaves) was studied with particular reference to their effectiveness for decreasing methane emission. The plant extracts (pine needles and ginkgo leaves) were added to an in vitro fermentation incubated with rumen fluid. The microbial population including bacteria, ciliated-associated methanogen, four different groups of methanogens and Fibrobacter succinogenes were quantified by using the real-time PCR. Gas profiles including methane, carbon dioxide and hydrogen, and runinal fermentation characteristics were observed in vitro. The methane emission from samples with an addition of individual juices from pine needles, ginkgo leaves and 70% ethanol extract from ginko leaves was significantly lower (p<0.05, 27.1, 28.1 and 28.1 vs 34.0 ml/g DM) than that of the control, respectively. Total VFAs in samples with an addition of any of the plant extracts were significantly lower than that of the control (p<0.05) as well. The order Methanococcales and the order Methanosarcinales were not detected by using PCR in any incubated mixtures. The ciliate-associated methanogens population decreased from 25% to 49% in the plant extacts as compared to control. We speculate that the supplementation of juice from pine needles and ginkgo leaves extract (70% ethanol extract) decreased the protozoa population resulting in a reduction of methane emission in the rumen and thus inhibiting methanogenesis. The order Methanobacteriales community was affected by addition of all plant extracts and decreased to less than the control, while the order Methanomicrobiales population showed an increase to more than that of the control. The F. succinogenes, the major fibrolytic microorganism, population in all added plant extracts was increased to greater than that of the control. In conclusion, pine needles and ginkgo leaves extracts appear to have properties that decrease methanogenesis by inhibiting protozoa species and may have a potential for use as additives for ruminants.

Detection of flavonoid compounds by cell culture of Ginkgo biloba L (은행(Ginkgo biloba L.)의 세포배양에 의한 Flavonoid류의 검출)

  • 김광수;백윤웅
    • KSBB Journal
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    • v.11 no.1
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    • pp.1-7
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    • 1996
  • Calli induced from Ginkgo bilha L. were cultured to investigate optimal culture conditions and identify the possibility production of useful compounds. Calli were obtained from leaves and stems of Ginkgo biloba seedlings and embryos on WP medium supplemented with 2mg/$\ell$ NAA and 5mg/$\ell$ kinetin. Chlorophyll-ricked green callus was inducted in MS liquid medium containing 1mg/$\ell$ NAA and 0.1mg/$\ell$ kinetin under light as 3 clones selected with origin. Embryo derived callus showed the highest growth rate. Analysis for flavonoids and their precursor was performed by TLC and EMS. A specific precursor of flavonoid was identified in callus, not in natural leaves. These findings indicate that tissue culture may produce rlavonoids.

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Identification and Quantitative Analysis of Flavonol Glycosides from Ginkgo biloba Leaves by High Performance Liquid Chromatography (고속액체크로마토그라피에 의한 은행잎중 Flavonoid Glycoside의 확인 및 정량)

  • Kang, Sam-Sik;Kim, Ju-Sun;Kwak, Wie-Jong;Kim, Ki-Hyup
    • Korean Journal of Pharmacognosy
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    • v.21 no.2
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    • pp.148-152
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    • 1990
  • Seven flavonol glycosides from the EtOAc fraction of Ginkgo biloba leaves were identified by high performance liquid chromatography. Separation by reversed phase chromatography on $Lichrosorb^{\circledR}$ RP-18 column was achieved by isocratic elution. The content of the major acylated flavonol glycoside, kaempferol 3-0-[$6'-O-{p}-coumaroyl-{\beta}-_D-glucosyl(1{\rightarrow}2)-{\alpha}-_L-rhamnoside$] was about 8.0% and 0.55% for EtOAc fraction and MeOH extract, respectively.

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Effects of Browning Reaction Products on DNA Damage (효소적 갈변 생성물의 DNA 손상에 대한 효과)

  • Lee, Ji-Eun;Kim, An-Keun
    • Korean Journal of Pharmacognosy
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    • v.31 no.2
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    • pp.240-244
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    • 2000
  • Antimutagenicity profiles of the enzymatic browning reaction products(EBRP) were investigated. The rec-assay with Bacillus subtilis strains $H17(rec^+)$ and $M45(rec^-)$ was carried out using their spores. The biological activities were evaluated for seven different enzymatic browning reaction products, which resulted from the reactions of seven polyphenols with polyphenol oxidase isolated from Ginkgo biloba leaves. In the spore $rec^-$ assay, most of the polyphenolic compounds tested were positive, whereas their enzymatic browning reaction products were tested negative. The mutagenicity of enzymic browning mixtures of the polyphenols and the enzymes obtained from Ginkgo biloba leaves showed negative results in the mutagenicity test using Bacillus subtilis strains $H17(rec^+)$ and $M45(rec^-)$. In the case where polyphenol oxidase inhibitors were added in the enzymatic reaction mixtures with polyphenols, the polyphenols showed mutagenic effect in the spore $rec^-$ assay. This suggests that the activity of polyphenol oxidase is decreased.

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Studies on the Extraction of Active Components in Ginkgo biloba Leaves by Enzyme Treatments (I) (효소처리에 의한 은행잎 중 활성성분 추출에 관한 연구( I ))

  • Kim, Bo-Young;Lee, Chang-Gurl;Hwang, Wan-Kyunn;Huh, Jae-Doo
    • Korean Journal of Pharmacognosy
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    • v.20 no.1
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    • pp.43-47
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    • 1989
  • An attempt was made to increase the yield of extraction of ginkgoflavonglycosides from leaves of Ginkgo biloba by treatments of with Cellulase C and macerating enzymes. The yield of dried extract and its contents of ginkgoflavonols, when treated only with cellulase C, were analyzed to be 1. 99% and 0. 38%, respectively. The contents of ginkgoflavonglycosides in the dried extracts were calculated to be 25. 28%. By the treatment with a mixture of three enzymes, cellulase C: cellulase NC and macerosin (1 : 1 : 2), the yield of the dried extract, ginkgoflavonols as well as their glycosides were determined to be 2. 48%, 0. 48% and 24. 16%, respectively.

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Seasonal Variations of the Flavonol Glycoside Content from Ginkgo biloba Leaves (은행잎중 Flavonol Glycoside 성분의 계절별 함량 변화에 관한 연구)

  • Kang, Gyu-Sun;Youm, Jeong-Rok;Kang, Sam-Sik
    • Korean Journal of Pharmacognosy
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    • v.24 no.1
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    • pp.47-53
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    • 1993
  • The seasonal variations of the major six flavonol glycosides(kaempferol 2,6-dirhamnosyl glucoside, quercetin 3-O-rutinoside, kaempferol 3-O-rutinoside, isorhamnetin 3-O-rutinoside, quercetin 3-O-coumaroyl glucorhamnoside and kaempferol 3-O-coumaroyl glucorhamnoside) in Ginkgo biloba leaves were investigated. The contents were determined by HPLC on reversed phase $C_{18}$ column. This result showed that the percentage of six flavonol glycosides decreased during the season from 1.57% in May to 0.39% in November. The content of each flavonol glycoside indicated a similar tendency to decrease. However, the contents of rutinosides of kaempferol, quercetin and isorhamnetin fluctuated markedly than those of coumaroyl glucorhamnosides of kaempferol and quercetin and kaempferol 2,6-dirhamnosyl glucoside.

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