Identification and Quantitative Analysis of Flavonol Glycosides from Ginkgo biloba Leaves by High Performance Liquid Chromatography

고속액체크로마토그라피에 의한 은행잎중 Flavonoid Glycoside의 확인 및 정량

  • Kang, Sam-Sik (Natural Products Research institute, Seoul National University) ;
  • Kim, Ju-Sun (Natural Products Research institute, Seoul National University) ;
  • Kwak, Wie-Jong (Life Science Research Center, Sunkyong Industries) ;
  • Kim, Ki-Hyup (Life Science Research Center, Sunkyong Industries)
  • 강삼식 (서울대학교 생약연구소) ;
  • 김주선 (서울대학교 생약연구소) ;
  • 곽의종 (선경인더스트리 생활과학연구소) ;
  • 김기협 (선경인더스트리 생활과학연구소)
  • Published : 1990.06.30

Abstract

Seven flavonol glycosides from the EtOAc fraction of Ginkgo biloba leaves were identified by high performance liquid chromatography. Separation by reversed phase chromatography on $Lichrosorb^{\circledR}$ RP-18 column was achieved by isocratic elution. The content of the major acylated flavonol glycoside, kaempferol 3-0-[$6'-O-{p}-coumaroyl-{\beta}-_D-glucosyl(1{\rightarrow}2)-{\alpha}-_L-rhamnoside$] was about 8.0% and 0.55% for EtOAc fraction and MeOH extract, respectively.

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