• 환경영향평가
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• 제30권3호
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• pp.164-174
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• 2021

개체수와 종수에 기초한 기존 생물다양성 평가와 달리, 계통적 다양성 평가는 계통유전적 다양성 및 생태적 다양성도 평가할 수 있다. 본 연구에서는 서울특별시 철새보호구역으로 지정되어 다년간의 생태모니터링이 용이한 한강의 주요 하천인 중랑천, 청계천 및 안양천의 지난 9년간의 겨울철 조류상 장기 모니터링 결과를 이용하여 기존의 다양성 평가와 계통적 다양성 평가를 각각 수행한 뒤 각 결과가 내포하는 정보에 대해 고찰했다. 분석결과 중랑천과 안양천은 전반적으로 조류 개체수가 시계열적으로 감소한 반면 청계천은 개체수 변동이 없었다. 종 풍부도는 청계천에서 시간에 따라 소폭 상승한 반면, 중랑천과 안양천은 연도별로 차이가 없었다. 기존 종 다양도는 안양천을 제외한 중랑천과 청계천에서 시간에 따라 증가했는데, 계통적 종 다양도는 청계천에서만 증가하는 추세를 보였다. 이러한 생물다양성 평가 지수의 변동은 각 조사구역 내에서 발생한 공사 등의 인위적 요인에 의한 것으로 판단되며, 종 다양도와 계통적 종 다양도는 같은 결과를 반영하지 않는다는 것을 보여준다. 따라서 본 연구는 생물다양성 평가에 있어 유전 및 생태적 관점과 같은 다양한 시각에서 고려할 필요가 있음을 시사한다.

• Asian-Australasian Journal of Animal Sciences
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• 제32권2호
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• pp.176-182
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• 2019

Objective: This study was conducted to investigate basic information on genetic structure and characteristics of Limousin population in Hungary. Obtained results will be taken into consideration when adopting the new breeding strategy by the Association of Hungarian Limousin and Blonde d'Aquitaine Breeders (AHLBB). Methods: Genetic diversity and phylogenetic relationship of 3,443 Limousin cattle from 16 different herds were investigated by performing genotyping using 18 microsatellite markers. Amplified DNA was genotyped using an automated genetic analyzer. Results: Mean of effective alleles ($n_e$) of the populations was 3.77. Population C had the lowest number of effective alleles (3.01) and the lowest inbreeding coefficient ($F_{IS}$) value (-0.15). Principal component analysis of estimated genetic distance ($F_{ST}$) values (p<0.000) revealed two herds (C and E) distinct from the majority of other Limousin herds. The pairwise $F_{ST}$ values of population C compared to the others (0.066 to 0.120) fell into the range of moderate genetic distance: 0.050 to 0.150, while population E displayed also moderate genetic distance ($F_{ST}$ values in range 0.052 to 0.064) but only to six populations (G, H, J, L, N, and P). $F_{ST(C-E)}$ was 0.148, all other pairs -excluding C and E herds- displayed low genetic distance ($F_{ST}$<0.049). Population D, F, I, J, K, L, N, O, and P carried private alleles, which alleles belonged to 1.1% of the individuals. Most probable number of clusters (K) were 2 and 7 determined by Structure and BAPS software. Conclusion: This study showed useful genetic diversity and phylogenetic relationship data that can be utilized for the development of a new breeding strategy by AHLBB. The results presented could also contribute to the proper selection of animals for further whole genome scan studies of Limousins.

• 한국미생물·생명공학회지
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• 제44권4호
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• pp.512-521
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• 2016

제주도에서 채집된 토양시료로부터 xylanase 활성을 나타내는 균주를 분리하여 WJ-2로 명명하였다. 균주 WJ-2의 16S rRNA 유전자 염기서열을 결정하여 이를 토대로 상동성을 검색한 결과, Streptomyces 속의 균주들과 높은 염기서열 상동성을 보였다. 16S rRNA 유전자 염기서열을 토대로하는 neighbor-joining 계통수를 제작하여 Streptomyces atrovirens와 가장 높은 계통발생적 연관성이 갖고 있는 것을 밝혔다. 또한 DNA-DNA hybridization 분석을 통하여 Streptomyces atrovirens의 신규한 아종임을 증명하였다. 균주 WJ-의 게놈내 GC 농도는 73.98 mol%이었으며, 주요 세포벽 지방산으로 anteiso-$C_{15:0}$ (36.19%)을 함유하고 있었다. 균주 WJ-2의 성장 및 xylanase 생산은 배지내에 질소원으로 soytone과 탄소원으로 xylan을 첨가하였을 때 급격히 증가되는 것을 확인하였다. 액체배양액으로부터 준비된 조효소의 xylanase 활성은 pH 7.0과 $55^{\circ}C$에서 가장 높게 나타났다. Thin layer chromatography (TLC) 분석을 통하여 균주 WJ-2의 조효소는 xylan을 분해하여 최종분해산물로서 xylobiose와 xylotriose 생산하는 효소임을 확인하였다.

• 한국식품영양과학회지
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• 제46권4호
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• pp.523-528
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• 2017

본 연구는 국내 산림자생 식물 추출물 5종(밤나무, 진달래, 산수유, 복분자딸기, 갈참나무)에 대한 지방세포 분화 억제 및 활성산소종(ROS) 생성 억제 효능을 평가하였다. 지방세포 분화 중 세포 내 지방축적 및 ROS 생성량을 비교한 결과, 국내 산림 식물 5종 모두 지방축적량 및 ROS 생성량을 농도 유의적으로 감소키는 것으로 나타났다. 특히 복분자딸기 추출물을 처리한 지방세포에서 지방 축적 및 ROS 저감에 가장 크게 나타났으며 양성대조군으로 사용된 N-acetyl-L-cysteine과 유사한 결과를 나타내었다. 또한, 복분자딸기 추출물을 처리함으로써 지방세포의 분화를 조절하는 전사인자인 $PPAR{\gamma}$, $C/EBP{\alpha}$ 및 aP2 발현이 억제됨을 확인하였다. 국내 산림 식물 5종 추출물은 천연물에서 유래한 항비만 및 항산화 활성 기능성식품 소재로 활용 가능할 것으로 생각한다.

• Nutrition Research and Practice
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• 제13권4호
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• pp.302-309
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• 2019

BACKGOURND/OBJECTIVES: Vascular inflammation is an important feature in the atherosclerotic process. Recent studies report that leaves and branches of Carpinus turczaninowii (C. turczaninowii) have antioxidant capacity and exert anti-inflammatory effects. However, no study has reported the regulatory effect of C. turczaninowii extract on the arterial inflammatory response. This study therefore investigated modulation of the arterial inflammatory response after exposure to C. turczaninowii extract, using human aortic vascular smooth muscle cells (HAoSMCs). MATERIALS/METHODS: Scavenging activity of free radicals, total phenolic content (TPC), cell viability, mRNA expressions, and secreted levels of cytokines were measured in LPS-stimulated (10 ng/mL) HAoSMCs treated with the C. turczaninowii extract. RESULTS: C. turczaninowii extract contains high amounts of TPC ($225.6{\pm}21.0mg$ of gallic acid equivalents/g of the extract), as well as exerts time-and dose-dependent increases in strongly scavenged free radicals (average $14.8{\pm}1.97{\mu}g/mL$ $IC_{50}$ at 40 min). Cell viabilities after exposure to the extracts (1 and $10{\mu}g/mL$) were similar to the viability of non-treated cells. Cytokine mRNA expressions were significantly suppressed by the extracts (1 and $10{\mu}g/mL$) at 6 hours (h) after exposure. Interleukin-6 secretion was dose-dependently suppressed 2 h after incubation with the extract, at $1-10{\mu}g/mL$ in non-stimulated cells, and at 5 and $10{\mu}g/mL$ in LPS-stimulated cells. Similar patterns were also observed at 24 h after incubation with the extract (at $1-10{\mu}g/mL$ in non-stimulated cells, and at $10{\mu}g/mL$ in the LPS-stimulated cells). Soluble intracellular vascular adhesion molecules (sICAM-1) secreted from non-stimulated cells and LPS-stimulated cells were similarly suppressed in a dose-dependent manner after 24 h exposure to the extracts, but not after 2 h. In addition, sICAM-1 concentration after 24 h treatment was positively related to IL-6 levels after 2 h and 24 h exposure (r = 0.418, P = 0.003, and r = 0.524, P < 0.001, respectively). CONCLUSIONS: This study demonstrates that C. turczaninowii modulates the arterial inflammatory response, and indicates the potential to be applied as a therapeutic use for atherosclerosis.

• 한국미생물·생명공학회지
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• 제50권4호
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• pp.557-562
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• 2022

본 연구에서는 토양, 담수 및 염수 퇴적물, 슬러지 등 8종의 접종원을 이용하여 배양인자(기질종류, 염농도, 배양온도)에 따른 바이오수소 생산 잠재능을 평가하고 최적조건을 도출하고자 하였다. 각 접종원 별 바이오수소 생산속도는 배양온도와 접종원 종류에 의해 유의한 영향을 받았다. 반면 기질종류와 염농도는 바이오수소 생산속도에 대체로 유의한 영향을 끼치지 않았다. 고온(50°)보다 중온(37℃)의 배양 온도가 수소 생산에 더 적합하였으며, 8종의 접종원 중에서 중에서는 혐기소화슬러지의 수소 생산능이 가장 우수하였다. 혐기소화슬러지의 최대 수소 생산속도는 중온(37℃)과 고온(50℃)에서 각각 2,729.0 및 1,384.7 ml-H₂·l^-1·d^-1였다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권17호
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• pp.7021-7027
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• 2014

Background: The problem of cancer, especially lung cancer, is very acute in Bangladesh. The present study was conducted to evaluate the risk of lung cancer among Bangladeshi people based on hereditary, socio-economic and demographic factors. Materials and Methods: This study was carried out in 208 people (patients-104, controls-104) from January 2012 to September 2013 using a structured questionnaire containing details of lung cancer risk factors including smoking, secondhand smoke, tobacco leaf intake, age, gender, family history, chronic lung diseases, radiotherapy in the chest area, diet, obesity, physical activity, alcohol consumption, occupation, education, and income. Descriptive statistics and testing of hypotheses were used for the analysis using SPSS software (version 20). Results: According to this study, lung cancer was more prevalent in males than females. Smoking was the highest risk factor (OR=9.707; RR=3.924; sensitivity=0.8872 and P<0.0001) followed by previous lung disease (asthma, tuberculosis etc.) (OR=7.095; RR=1.508; sensitivity=0.316 and P<0.0001)) for male patients. Highly cooked food (OR=2.485; RR=1.126; sensitivity=0.418 and P=0.004)) and also genetic inheritance (OR=1.93; RR=1.335; sensitivity=0.163 and P=0.138) demonstrated significant correlation with lung cancer as risk factors after these two and alcohol consumption was not prevalent. On the other hand, for female patients, tobacco leaf intake represented the highest risk (OR=2.00; RR=1.429; sensitivity= 0.667 and P=0.5603) while genetic inheritance and highly cooked food also correlate with lung cancer but not so significantly. Socioeconomic status and education level also play important roles in causing lung cancer. Some 78.5% male and 83.3% of female cancer patients were rural residents, while 58.2% lived at the margin or below the poverty line. Most male (39.8%) and female (50.0%) patients had completed only primary level education, and 27.6% male and 33.3% female patients were illiterate. Smoking was found to be more prevalent among the less educated persons. Conclusions: The results obtained in this study indicate the importance of creating awareness about lung cancer risk factors among Bangladeshi people and making appropriate access to health services for the illiterate, poor, rural people.

• 환경영향평가
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• 제14권4호
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• pp.157-164
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• 2005

Existing models that predict of Daily water supply include statistical models and neural network model. The neural network model was more effective than the statistical models. Only neural network model, which predict of Daily water supply, is focused on estimation of the operational control. Neural network model takes long learning time and gets into local minimum. This study proposes Neuro Genetic hybrid model which a combination of genetic algorithm and neural network. Hybrid model makes up for neural network's shortcomings. In this study, the amount of supply, the mean temperature and the population of the area supplied with water are use for neural network's learning patterns for prediction. RMSE(Root Mean Square Error) is used for a MOE(Measure Of Effectiveness). The comparison of the two models showed that the predicting capability of Hybrid model is more effective than that of neural network model. The proposed hybrid model is able to predict of Daily water, thus it can apply real time estimation of operational control of water works and water drain pipes. Proposed models include accidental cases such as a suspension of water supply. The maximum error rate between the estimation of the model and the actual measurement was 11.81% and the average error was lower than 1.76%. The model is expected to be a real-time estimation of the operational control of water works and water/drain pipes.

• 대한수학교육학회지:학교수학
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• 제11권3호
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• pp.431-462
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• 2009

본 연구에서는 지수의 역으로 정의되는 로그를 학습한 학생들을 대상으로 로그 개념과 성질에 대한 오류를 조사하고, 학생들 스스로 오류를 교정할 수 있도록 역사 발생적 수학 학습 지도 원리에 따른 교수-학습 모듈(module)을 개발하여 수행평가로 적용하고 그 교수학적 효과를 분석하였다. 교수-학습 모듈은 로그에 대한 수학적 분석, 역사발생적 과정에 대한 분석, 학생들의 오류에 대한 심리학적 분석을 바탕으로 로그의 정의와 기본 성질 단원을 중심으로 개발하였다. 교수-학습 모듈의 교수학적 효과는 로그의 뜻과 성질에 대한 사전 사후 지필 평가 결과의 정답률과 오류 발생률을 비교 분석하였다. 구체적으로 유의수준 .05에서 단일표본에 대한 t-검정 결과, 교수-학습 모듈을 적용한 수행평가가 짧은 시간동안 이루어졌음에도 효과가 있는 것으로 나타났다. 그리고 로그의 뜻과 성질 단원에서 나타난 5가지 오류 유형인 '기호나 용어, 개념에 대한 이해 부족으로 인한 오류(E1)', '정리나 성질에 대한 불완전한 이해로 인한 오류(E2)'와 '선행 지식의 부족으로 인한 오류(E3)', '계산 실수로 인한 오류(E4)', '풀이 과정의 중단으로 인한 오류(E5)' 중 교수-학습 모듈을 적용한 결과, E2와 E1 오류가 크게 개선되었다.

• Journal of Ecology and Environment
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• 제35권1호
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• pp.27-34
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• 2012

Typha angustifolia has ecological characteristics of clonal growth similar to Phragmites australis. The plant spreads byclonal growth and seed dispersal. In this study, for the three stands which have different settlement age at the Baksilji wetland in Korea, genetic diversity was estimated by random amplification of polymorphic DNA analysis to evaluate the change in genetic diversity of T. angustifolia during stand development in the same population. Stand (ST) 1 was the oldest and ST 4 was the youngest. ST 5 was in a small ditch out of the Baksilji. Although the ST 1, ST 2, and ST 3 did not differ significantly in vegetational or physical environment, the genetic diversity estimated according to Nei's gene diversity (h) and the Shannon index (i) increased in the order of ST 1 < ST 2 < ST 3 contrary to formative age. The genetic diversity of ST 4 was much higher than that of the other three stands. ST 4 has similar abiotic environmental conditions with slight T. angustifolia dominance, and seems to be in the early establishment stage. ST 5 differed from the other stands in vegetational and soil environments, which can result in stressful cattail conditions. Even though the ST 5 stand was not younger than the ST 4 stand, ST 5 showed the highest genetic diversity. Our results indicate that after early settlement of the T. angustifolia population, genetic diversity within the species decreased over time and that the decreasing pattern of genetic diversity within T. angustifolia stands is not likely to occur under stressful conditions.