Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Evaluation of Biohydrogen Production Using Various Inoculum Sources

다양한 접종원을 이용한 바이오수소 생산 평가

  • Geumhee, Kim (Department of Environmental Science and Engineering, Ewha Womans University) ;
  • Jiho, Lee (Department of Environmental Science and Engineering, Ewha Womans University) ;
  • Hyoju, Yang (Department of Environmental Science and Engineering, Ewha Womans University) ;
  • Yun-Yeong, Lee (Department of Environmental Science and Engineering, Ewha Womans University) ;
  • Yoonyong, Yang (Biological and Genetic Resources Assessment Division, National Institute of Biological Resources) ;
  • Sungho, Choi (Biological and Genetic Resources Assessment Division, National Institute of Biological Resources) ;
  • Moonsuk, Hur (Biological and Genetic Resources Assessment Division, National Institute of Biological Resources) ;
  • Byounghee, Lee (Biological and Genetic Resources Assessment Division, National Institute of Biological Resources) ;
  • Kyung-Suk, Cho (Department of Environmental Science and Engineering, Ewha Womans University)
  • 김근희 (이화여자대학교 환경공학과) ;
  • 이지호 (이화여자대학교 환경공학과) ;
  • 양효주 (이화여자대학교 환경공학과) ;
  • 이윤영 (이화여자대학교 환경공학과) ;
  • 양윤용 (국립생물자원관 유용자원분석과) ;
  • 최성호 (국립생물자원관 유용자원분석과) ;
  • 허문석 (국립생물자원관 유용자원분석과) ;
  • 이병희 (국립생물자원관 유용자원분석과) ;
  • 조경숙 (이화여자대학교 환경공학과)
  • Received : 2022.10.20
  • Accepted : 2022.11.30
  • Published : 2022.12.28
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Abstract

In this study we evaluated biohydrogen production potential as operational parameters (substrate, salt concentration, and temperature) using eight inoculum sources. While the volumetric biohydrogen production rate was significantly affected by temperature and inoculum sources, substrate and salt concentration did not have a significant effect on the biohydrogen production. Mesophilic temperature (37℃) was also found more appropriate for the hydrogen production than thermophilic temperature (50℃). Rate, while the eight inoculum sources, anaerobic digestion sludge exhibited the fastest biohydrogen production. The maximum production rate from anaerobic digestion sludge was 2,729 and 1,385 ml-H2·l-1·d-1 at mesophilic and thermophilic temperature, respectively.

본 연구에서는 토양, 담수 및 염수 퇴적물, 슬러지 등 8종의 접종원을 이용하여 배양인자(기질종류, 염농도, 배양온도)에 따른 바이오수소 생산 잠재능을 평가하고 최적조건을 도출하고자 하였다. 각 접종원 별 바이오수소 생산속도는 배양온도와 접종원 종류에 의해 유의한 영향을 받았다. 반면 기질종류와 염농도는 바이오수소 생산속도에 대체로 유의한 영향을 끼치지 않았다. 고온(50°)보다 중온(37℃)의 배양 온도가 수소 생산에 더 적합하였으며, 8종의 접종원 중에서 중에서는 혐기소화슬러지의 수소 생산능이 가장 우수하였다. 혐기소화슬러지의 최대 수소 생산속도는 중온(37℃)과 고온(50℃)에서 각각 2,729.0 및 1,384.7 ml-H2·l-1·d-1였다.

Keywords

Acknowledgement

This work was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR202221103).

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