• Animal Bioscience
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• v.35 no.10
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• pp.1592-1605
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• 2022

Objective: The objective of this study was to select an effective in vitro digestion-fermentation model to estimate the effect of decreasing dietary crude protein (CP) on odor emission during pig production and to suggest potential prediction markers through in vitro and in vivo experiments. Methods: In the in vitro experiment, three diet formulations with different CP contents (170 g/kg, 150 g/kg, and 130 g/kg) but containing the same standardized ileal digestible essential amino acids (SID-EAA) were assessed. Each diet was evaluated by two different in vitro gastric-intestinal phase digestion methods (flask and dialysis), combined with fresh pig feces-ferment inoculation. Eighteen growing barrows (31.9±1.6 kg) were divided into three groups: control diet (180 g CP/kg, without SID-EAA adjustment), 170 g CP/kg diet, and 150 g CP/kg diet for 4 weeks. Results: The in vitro digestion results indicated that in vitro digestibility was affected by the gastric-intestinal phase digestion method and dietary CP level. According to the gas kinetic and digestibility results, the dialysis method showed greater distinguishability for dietary CP level adjustment. Nitrogen-related odor compounds (NH₃-N, indole, p-cresol, and skatole) were highly correlated with urease and protease activity. The feeding study indicated that both EAA-adjusted diets resulted in a lower odor emission especially in p-cresol and skatole. Both protease and urease activity in feces were also closely related to odor emissions from nitrogen metabolism compounds. Conclusion: Dialysis digestion in the gastric-intestinal phase followed by fresh fecal inoculation fermentation is suitable for in vitro diet evaluation. The enzyme activity in the fermentation and the fecal samples might provide a simple and effective estimation tool for nitrogen-related odor emission prediction in both in vitro and in vivo experiments.

• Korean Journal of Exercise Nutrition
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• v.23 no.4
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• pp.26-31
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• 2019

[Purpose] Numerous epidemiological studies have shown that it is possible to prescribe exercise for neurodegenerative disease, such as Alzheimer's disease and Parkinson's disease. However, despite the availability of diverse scientific knowledge, the effects of exercise in this regard are still unclear. Therefore, this study attempted to investigate a substance, such as black chokeberry (Aronia melanocapa L.) that could improve the ability of the treatment and enhance the benefits of exercising in neurodegenerative diseases. [Methods] The cell viability was tested with 2,3-bis[2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolim-5-carboxanilide and the cells were stained with ethidium homodimer-1 solution. The mRNA expression levels were evaluated by microarray. The active compounds of black chokeberry ethanolic extract (BCE) were analyzed by gas chromatography. The chemical shift analysis in the brain was performed using magnetic resonance spectroscopy. [Results] BCE treatment decreased hydrogen peroxide-induced L6 cell death and beta amyloid induced primary neuronal cell death. Furthermore, BCE treatment significantly reduced the mRNA levels of the inflammatory factors, such as IL-1α, Cxcl13, IL36rn, Itgb2, Epha2, Slamf8, Itgb6, Kdm6b, Acvr1, Cd6, Adora3, Cd27, Gata3, Tnfrsf25, Cd40lg, Clec10a, and Slc11a1, in the primary neuronal cells. Next, we identified 16 active compounds from BCE, including D-mannitol. In vivo, BCE (administered orally at a dosage of 50 mg/kg) significantly regulated chemical shift in the brain. [Conclusion] Our findings suggest that BCE can serve as a candidate for neurodegenerative disease therapy owing to its cyto-protective and anti-inflammatory effects. Therefore, BCE treatment is expected to prevent damage to the muscles and neurons of the athletes who continue high intensity exercise. In future studies, it would be necessary to elucidate the effects of combined BCE intake and exercise.

• Journal of Environmental Science International
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• v.33 no.8
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• pp.575-581
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• 2024

Insect pests are a significant threat to stored crops and can lead to considerable economic losses and reduced crop quality. Traditional pest control methods often involve chemical treatments, which have adverse environmental and health effects. This study evaluated the effectiveness of controlling the environment using LED lighting and plant-derived odor barriers as a dual strategy for insect pest control. The storage environment was altered using LED lights that emitted specific wavelengths (580-585 nm) and by reducing other wavelengths (300-500 nm). This light environment was combined with an insect odor barrier derived from Cinnamomum verum, Illicium verum, and Artemisia annua, and their duel impact on the behavior and frequency of insect pests under real storage conditions was determined. The findings revealed significant changes in the frequencies of various insect orders, indicating differential responses to light wavelengths and odor barriers. Notably, the introduction of an anti-insect light and odor barrier environment reduced Diptera and Hemiptera frequencies, which could potentially reduce pest intrusion. The results underscore the potential use of integrated light and odor barriers as a noninvasive and environmentally friendly approach to pest management. This study identifies the specific wavelengths and odor combinations that effectively deter insect pests and contribute to the development of more efficient and sustainable pest control methods. It also highlights the importance of understanding insect behavior and frequency changes in response to novel deterrent strategies.

• Journal of Korean Society of Transportation
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• v.17 no.4
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• pp.99-110
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• 1999

With a growing awareness, transportation is the maior source of urban air pollution. Planners and policy-makers are strongly urged to care the impacts of transportation management strategies on environment. Since transport-related air Pollution can be regarded as negative externalities of transport, it is indispensable to evaluate chanties in air quality which seems to occur when proposed transport measures are implemented. In addition, transport measures should be integrated in a set of combined Paradigm regarding transport and environment. Based on the integrated Paradigm between transportation demand management(TDM) and environmental planning, the main focus is given to apply environment cost internalizing measures to the short-term congestion management Program(SCMP) in Seoul, that has been developed in 1998 by Seoul Development Institute. Three modules are analyzed: without and/or with ￦500, ￦1000 emission plus gas tax, respectively. From the empirical applications on Seoul city, one of the most exemplary findings in SCMP program is that, emission plus gas tax can be a very useful measure to reduce vehicular emissions by targeting major Pollutants differently, rather than by dealing with pollutants collectively. Further research that provides (1)showing the spatial variation of pollution levels along the intersections, (2)more developing combined Paradigm between transport, land use, and environment, (3)using environmental road capacity rather than Physical-aspects of road capacity, and (4)continuing R&D on air quality constrained TDM, can contribute significantly to applications of the real and efficient environment-constrained transportation planning.

• Korean Journal of Remote Sensing
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• v.37 no.5_2
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• pp.1295-1305
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• 2021

Satellite-derived ocean color products are required to effectively monitor clear open ocean and coastal water regions for various research fields. For this purpose, accurate correction of atmospheric effect is essential. Currently, the Geostationary Ocean Color Imager (GOCI)-II ground segment uses the reanalysis of meteorological fields such as European Centre for Medium-Range Weather Forecasts (ECMWF) or National Centers for Environmental Prediction (NCEP) to correct gas absorption by water vapor and ozone. In this process, uncertainties may occur due to the low spatiotemporal resolution of the meteorological data. In this study, we develop water vapor absorption correction model for the GK-2 combined GOCI-II atmospheric correction using Advanced Meteorological Imager (AMI) total precipitable water (TPW) information through radiative transfer model simulations. Also, we investigate the impact of the developed model on GOCI products. Overall, the errors with and without water vapor absorption correction in the top-of-atmosphere (TOA) reflectance at 620 nm and 680 nm are only 1.3% and 0.27%, indicating that there is no significant effect by the water vapor absorption model. However, the GK-2A combined water vapor absorption model has the large impacts at the 709 nm channel, as revealing error of 6 to 15% depending on the solar zenith angle and the TPW. We also found more significant impacts of the GK-2 combined water vapor absorption model on Rayleigh-corrected reflectance at all GOCI-II spectral bands. The errors generated from the TOA reflectance is greatly amplified, showing a large error of 1.46~4.98, 7.53~19.53, 0.25~0.64, 14.74~40.5, 8.2~18.56, 5.7~11.9% for from 620 nm to 865 nm, repectively, depending on the SZA. This study emphasizes the water vapor correction model can affect the accuracy and stability of ocean color products, and implies that the accuracy of GOCI-II ocean color products can be improved through fusion with GK-2A/AMI.

• Korean Chemical Engineering Research
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• v.44 no.4
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• pp.356-362
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• 2006

The $SO_2$ reduction using CO and $H_2$ over Sn-Zr based catalysts was performed in this study. Sn-Zr based catalysts with Sn/Zr molar ratio (0/1, 1/4, 1/1, 2/1, 3/1, 1/0) were prepared by the precipitation and co-precipitation method. The effect of the temperature on the reaction characteristics of the $SO_2$ reduction with a reducing agent such as $H_2$ and CO was investigated under the conditions of space velocity of $10,000ml/g_{-cat.}h$, $([CO(or\;H_2)]/[SO_2])$ of 2.0. As a result, the activity of Sn-Zr based catalysts were higher than $SnO_2$ and $ZrO_2$. The reactivity for the $SO_2$ reduction with CO was higher than that with $H_2$, and sulfur yield in the $SO_2$ reduction by $H_2$ was higher than that by CO. The reactivity for the $SO_2$ reduction with $H_2$ was increased with the reaction temperature regardless of Sn-Zr based catalyst with a Sn/Zr molar ratio. $SnO_2-ZrO_2$ (Sn/Zr=1/4) had highest activity at $550^{\circ}C$, in the $SO_2$ reduction with $H_2$ and $SO_2$ conversion of 94.4％ and sulfur yield of 66.4％ were obtained at $550^{\circ}C$. On the other hand, in the $SO_2$ reduction by CO, the reactivity was decreased with the increase over $325^{\circ}C$. At the optimal temperature of $325^{\circ}C$, $SO_2$ conversion and sulfur yield were about 100％ and 99.5％, respectively, in the $SO_2$ reduction over $SnO_2-ZrO_2$ (Sn/Zr=3/1). Also, the $SO_2$ reduction using syngas with $CO/H_2$ ratio over $SnO_2-ZrO_2$ (Sn/Zr=2/1) was performed in order to investigate the application possibility of the simulated coal gas as the reductant in DSRP. As a result, the reactivity of the $SO_2$ reduction using syngas with $CO/H_2$ ratio was increased with increasing the CO content of syngas. Therefore, it could be known that DSRP using the simulated coal gas over Sn-Zr based catalyst is possible to be realized in IGCC system

• Journal of Plant Biotechnology
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• v.42 no.1
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• pp.60-70
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• 2015

The aim of this study was to investigate whether fourier transform infrared (FT-IR) spectroscopy can be applied to simultaneous determination of fatty acids contents in different soybean cultivars. Total 153 lines of soybean (Glycine max Merrill) were examined by FT-IR spectroscopy. Quantification of fatty acids from the soybean lines was confirmed by quantitative gas chromatography (GC) analysis. The quantitative spectral variation among different soybean lines was observed in the amide bond region ($1,700{\sim}1,500cm^{-1}$), phosphodiester groups ($1,500{\sim}1,300cm^{-1}$) and sugar region ($1,200{\sim}1,000cm^{-1}$) of FT-IR spectra. The quantitative prediction modeling of 5 individual fatty acids contents (palmitic acid, stearic acid, oleic acid, linoleic acid, linolenic acid) from soybean lines were established using partial least square regression algorithm from FT-IR spectra. In cross validation, there were high correlations ($R^2{\geq}0.97$) between predicted content of 5 individual fatty acids by PLS regression modeling from FT-IR spectra and measured content by GC. In external validation, palmitic acid ($R^2=0.8002$), oleic acid ($R^2=0.8909$) and linoleic acid ($R^2=0.815$) were predicted with good accuracy, while prediction for stearic acid ($R^2=0.4598$), linolenic acid ($R^2=0.6868$) had relatively lower accuracy. These results clearly show that FT-IR spectra combined with multivariate analysis can be used to accurately predict fatty acids contents in soybean lines. Therefore, we suggest that the PLS prediction system for fatty acid contents using FT-IR analysis could be applied as a rapid and high throughput screening tool for the breeding for modified Fatty acid composition in soybean and contribute to accelerating the conventional breeding.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.11
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• pp.1655-1661
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• 2005

The present studies were designed to provide new information on fatty acid profiles of various muscles and adipose tissues of fattening horses in comparison with beef cattle and pigs. In the first study, the lipids were extracted respectively from subcutaneous, intermuscular adipose tissues, longissimus dorsi and biceps femoris muscles of fattening Breton horses (n = 8) with an average body weight of 1,124 kg. In the second study, the lipids were extracted from subcutaneous, intermuscular adipose tissues and longissimus dorsi muscle of fattening horses (n = 13), Japanese Black beef cattle (n = 5), Holstein steers (n = 5) and fattening pigs (n = 5). The fatty acids in the lipid samples were determined by gas chromatography after methylation by a combined base/acid methylation method. It was found that the lipids from horse subcutaneous and intermuscular adipose tissues contained more (p<0.05) polyunsaturated fatty acids (PUFA) which were mainly composed of linoleic acid (C18:2) and linolenic acid (C18:3) than those in the muscles. The weight percent of conjugated linoleic acids (CLA cis 9, trans 11) in lipids from biceps femoris muscle was 0.22%, which was higher (p<0.05) than that from the other depots. The horse lipids were higher (p<0.05) in PUFA but lower (p<0.05) in SFA and MUFA in comparison with those of the cattle and pigs. The percentage of C18:2 or C18:3 fatty acid in the horse lipids were respectively 2-8 fold or 5-18 fold higher (p<0.05) than those of the cattle and pigs. The percentages of CLA (cis 9, trans 11) in the horse lipids (0.14-0.16%) were very close to those of the pigs (0.18-0.19%) but much lower (p<0.05) than those of the Japanese Black beef cattle (0.55-0.94%) and Holstein steers (0.46-0.71%). The results indicated that the fatty acid profiles of lipids from different muscle and adipose tissues of fattening horses differed significantly. In comparison with that of the beef cattle and pigs, the horse lipids contained more C18:2 and C18:3 but less CLA.

• Horticultural Science & Technology
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• v.19 no.4
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• pp.550-554
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• 2001

'Fuyu' persimmon fruits (Diospyros kaki Thunb.) were seal-packaged in $60{\mu}m$ polyethylene (PE) film bags with various $O_2$ and $CO_2$ combinations (0% $O_2$+5% $CO_2$, 2% $O_2$+5% $CO_2$, 2% $O_2$+8% $CO_2$, 5% $O_2$+5% $CO_2$, 5% $O_2$+10% $CO_2$), and in vacuum and air. They were then stored at $-0.5^{\circ}C$ for 95 days. Changes in fruit weight, firmness, soluble solids, Hunter L and a values, $CO_2$ concentrations, and marketability were investigated at 20-day intervals. A combination of 2% $O_2$ and 5% $CO_2$ as well as the vacuum treatment were effective in maintaining firmness, soluble solids, marketability, and Hunter a value of 'Fuyu' fruits. On the other hand, the treatment of 2% $O_2$ combined with 8% $CO_2$ and vacuum treatment were effective in maintaining fresh weight and Hunter L and a values. The highest marketable quality was maintained when the fruits were vacuum-sealed, although fruits softened severely in the area where two fruits contacted with each other.

• The Korean Journal of Mycology
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• v.43 no.3
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• pp.200-205
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• 2015

Perilla frutescens var. japonica Hara was collected from farmland in Seongju-gun. Fifteen endophytic fungal strains with different colony morphologies were isolated from roots of P. frutescens. Waito-c rice seedlings were treated with the concentrated culture filtrates (CF) of endophytic fungi for observation of their plant growth-promoting activities. In the results, the CF of Y2H001 fungal strain promoted the growth of the waito-c rice seedlings. The phylogenetic tree of Y2H001 strain was analyzed by the combined sequences of the partial internal transcribed spacer region (ITS) and partial betatubulin gene. Molecular and morphological studies identified the Y2H001 strain as belonging to Aspergillus flavus. In gas chromatography mass spectrometry (GC/MS) analysis of the CF of Y2H001 strain, gibberellic acid (GA) was detected and quantified. Therefore, we describe Y2H001 strain as a new $GA_3$-producing A. flavus based on morphological, molecular characteristics and analysis of secondary metabolite.