• Bulletin of Food Technology
• /
• v.17 no.1
• /
• pp.69-78
• /
• 2004

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2021.10a
• /
• pp.226-226
• /
• 2021

• Journal of Plant Biotechnology
• /
• v.36 no.2
• /
• pp.106-114
• /
• 2009

Genetic components introduced into approved GM crops are a key subject for safety assessment and provide a basis for the development of detection methods for GM crops. In order to understand the genetic components in approved GM crops comprehensively, we screened the genetic vector maps of GM crops that had been approved for commercialization around the world. A total of 64 varieties from 5 major GM crop species (maize, canola, cotton, soybean, and tomato) were subjected to analysis. The genetic components included genes, promoters, terminators, and selection marker. This survey may be useful for researchers who develop GM crops and methods for detecting GM crops.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.350-350
• /
• 2017

The study was conducted to evaluate differences of growth characteristics of rice cultivated in two different regions (Cheonan and Jeonju). It focused on nutritional composition and anti-nutritional factors of rice straw produced from 21 rice varieties including GM rice (Iksan 483). The range of general nutrition ingredient is that crude was 0.97 ~ 3.2 %, carbohydrate was 67.45 ~ 80.01 %, crude protein was 1.46 ~ 4.81 %, crude ash was 6.52 ~ 18.96 %, crude fiber was 25.77 ~ 40.02 %, NDF was 51.84 ~ 67.77 %, ADF was 27.11 ~ 40.44 %, calcium was 0.49 ~ 5.18 mg/g and phosphorous was 0.26 ~ 2.77 mg/g. The general nutritional contents of GM rice were included above range. The range of phytic acid of rice straws cultivated in Cheonan and Jeonju was 0 ~ 0.056 mg/ml and 0 ~ 0.059 mg/ml, respectively. The phytic acid content of GM was 0.033 mg/ml, which was in the range of the content of rice straw in Cheonan and Jeonju. The range of trypsin inhibitor of rice straws cultivated in Cheonan and Jeonju was 0.061 ~ 0.461 TIU/mg and 0 ~ 1.278 TIU/mg, respectively. The trypsin acid content of GM was 0.461 TIU/mg, which was in the range of the content of rice straw in Cheonan and Jeonju. In addition, we investigated microbial community from each soil sample by using metagenomics sequencing based on rRNA microbial diversity in order to inspect indirect changes of soil environment with cultivation of GM rice. Metagenomics analysis was carried out using soil samples cultivated with GM and non-GM rice for before transplanting, young panicle differentiation stage, heading stage, and ripening stage. Beta diversity of microbial community in both soil environments were calculated by using Bray-Curtis distance method and showed low value with an average of 0.24 (dissimilarity = 1). As a result, it was confirmed that the cultivation of GM does not give a significant effect on the change of microbial composition in soil. Therefore, Our study demonstrates that there is no difference in the composition of soil microorganism due to GM and non-GM rice.

• Life and Agrochemicals
• /
• v.27 no.4 s.217
• /
• pp.36-38
• /
• 2006

중국, 對일 농산물 수출기업 대상 연수 실시/ 세계 농약시장, 3년 연속 증가/ 일본, 지역특산물에 대한 농약관리법 특례 종료/ 中상해시, 오염된 밭 농산물 생산 금지/ 영국, 농약사용 규정 개정/ 美소비자단체, GM작물 철회 위한 농무성 소송/ 中운남성 중소기업, 메콩강 유역국가와 경제관계 강화

• Korean Journal of Food Science and Technology
• /
• v.36 no.4
• /
• pp.677-682
• /
• 2004

Debates on safety of genetically modified (GM) crops have led to mandatory-labeling legislation of GM foods in many countries including Korea. Effects of heat, pressure, and acid treatments on degradation of DNAs or proteins in GM soybean at levels below detection limits of qualitative PCR and lateral flow strip test (LFST) methods were examined. Results showed that genomic DNAs and proteins were degraded into fragment sizes no longer possible for detection of inserted gene depending on thermal, or thermal and pressure treatment period. Detectaability of LFST for toasted meal increased in weakly treated soybean. DNA and protein detection methods were barely effective for detection of GM ingredient after $121^{\circ}C$ and 1.5 atmospheric treatment for 20 min. These results will be useful in determining GM labeling requirements of processed foods.

• Korean Journal of Breeding Science
• /
• v.42 no.5
• /
• pp.540-546
• /
• 2010

This study was conducted to understand the role of soybean pathogenesis-related 10 (GmPR10) gene in salt tolerance and to develop salt-tolerant rice using GmPR10 cDNA. GmPR10 transgene was expressed constitutively in the shoot and root of the $T_1$ transgenic rice plants. Interestingly, however, the levels of the transgene expression were increased temporally up to over four- to five-fold in the shoot and root by 125 mM NaCl treatment, peaking at six hours after the treatment and decreasing thereafter. Electrolyte leakage of leaf cells under 125 mM NaCl treatment was lower in all the transgenic lines than in the control variety, Dongjin-byeo. Ability of seedlings to recover from 125 mM NaCl treatment for two weeks was higher in the transgenic plants than in the control plants. These results demonstrated that GmPR10 had function to increase cell integrity and promote growth under the saline stress imposed by NaCl. The transgenic line GmPR10-3 which showed highest ability to recover from the saline stress could be used as a potential source for salt tolerance in rice breeding programs.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.48 no.2
• /
• pp.89-95
• /
• 2003

The study of this experiment is focused on labor saving of healthy rice seedling production using PSF (polypropylene spunbonded fabrics) as thermal protection material. Several factors such as different compositions of nursery soil and PSF materials were tested to produce healthy rice seedlings. The inner thermal protection material in PE film (polyethylene film) showed $0.9-1.7^{\circ}C$ higher than that of PSF 40-100 $\textrm{gm}^{-2}$. The light transmittance-ratio also showed similar trends. It is considered that the appropriate PSF material density was 40 gm$\textrm{gm}^{-2}$ in accordance with economic values and healthy rice seedlings. Plant height and dry weight according to various nursery soil showed the rang-es of 8.5-14.2cm and 5.5-10.0mg, respectively. In composition of nursery soil, artificial soil combined with paddy soil was effective in producing healthy seedling for rice seedling production. The total sugar content also showed the difference between PSF 40, 60 $\textrm{gm}^{-2}$ PE film (0.43-0.52mg FW $\textrm{g}^{-1}$) and PSF 80, 100 $\textrm{gm}^{-2}$ (0.28-0.35mg FW $\textrm{g}^{-1}$) and it showed the same tendency among varieties as well as various nursery soil. These results demonstrate that PSF 40 $\textrm{gm}^{-2}$ economically affordable, and can be recommended as thermal protection material for producing good healthy rice seedling.

• Korean Journal of Food Science and Technology
• /
• v.36 no.5
• /
• pp.828-832
• /
• 2004

Enzyme-linked immune sorbent assay (ELISA) was applied to quantify soy protein in fresh soybean curd (bean curd) produced by combination of genetically modified (GM) and genetically not modified (non-GM) soybeans. Antibodies against 113 and 24 kDa proteins, which appeared only in non-GM bean curd (specific band), and in both non-GM and GM bean curds (non-specific band) based on SDS-PAGE results, were prepared by immunization to rabbit. Through ELISA using either antibody, GM bean curd protein content was determined at dilution rates of $10^{-1}-10^{-6}$. Standard curve showing relationship between ELISA optical density and non-GM protein content was produced using antibody against 113 kDa protein at protein dilution between $10^{-7}\;to\;10^{-6}$, highly antigen content-dependent dilution. Bean curd prepared by random combinations of GM and non-GM soybeans were analyzed by ELISA, and standard curve was produced. Results reveal non-GM protein content of bean curd could be quantified with higher than 93% accuracy.

• Korean Journal of Medicinal Crop Science
• /
• v.12 no.1
• /
• pp.24-30
• /
• 2004

The immune activities of Ganoderma lucidum Mycelium added garlic extracts (GAM), Ganoderma lucidum Mycelium (GM), garlic extracts (GS) and standard $({\beta}-glucan)$ were compared. GAM enhanced the growth of human immune T cell up to $1.25{\sim}1.46$ times, compared to control group. GAM showed relatively lower cytotoxicity in using normal human lung cell, while GAM showed the most potent inhibitory effect on the human lung carcinoma, compared to GM and GS. The selectivity of GAM was also higher than that of GM and GS. GAM increased the secretion of cytokines, IL-6 and TNF- from human B cell as well as the growth of human immune cells. It can imply that GAM has higher immune activity than GM or GS.