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Development of Salt-Tolerant Transgenic Rice Using Soybean PR10 Gene  

Kim, Hyo Jin (Department of Crop Science, Chonbuk National University)
Baek, So Hyeon (Planning and Coordination Division, NICS, RDA)
Shin, Woon Chul (Department of Rice and Winter Cereal Crop, NICS, RDA)
Seo, Chun Sun (Department of Rice and Winter Cereal Crop, NICS, RDA)
Park, Myoung Ryoul (Institute of Agricultural Science and Technology)
Ko, Jae Kwon (Department of Rice and Winter Cereal Crop, NICS, RDA)
Yun, Song Joong (Department of Crop Science, Chonbuk National University)
Publication Information
Korean Journal of Breeding Science / v.42, no.5, 2010 , pp. 540-546 More about this Journal
Abstract
This study was conducted to understand the role of soybean pathogenesis-related 10 (GmPR10) gene in salt tolerance and to develop salt-tolerant rice using GmPR10 cDNA. GmPR10 transgene was expressed constitutively in the shoot and root of the $T_1$ transgenic rice plants. Interestingly, however, the levels of the transgene expression were increased temporally up to over four- to five-fold in the shoot and root by 125 mM NaCl treatment, peaking at six hours after the treatment and decreasing thereafter. Electrolyte leakage of leaf cells under 125 mM NaCl treatment was lower in all the transgenic lines than in the control variety, Dongjin-byeo. Ability of seedlings to recover from 125 mM NaCl treatment for two weeks was higher in the transgenic plants than in the control plants. These results demonstrated that GmPR10 had function to increase cell integrity and promote growth under the saline stress imposed by NaCl. The transgenic line GmPR10-3 which showed highest ability to recover from the saline stress could be used as a potential source for salt tolerance in rice breeding programs.
Keywords
Electrolyte leakage; Pathogenesis resistance; Rice; Salt tolerance; Transformation;
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